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71.
Freitag T Squires RA Schmid J Elliott J Rycroft AN 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2006,20(2):245-249
Older cats with chronic renal failure (CRF) commonly develop urinary tract infections (UTI). Uropathogenic Escherichia coli (UPEC) is identified as the causal agent of UTI in most affected cats. Infections are often complicated, and UPEC infections may persist or recur in these cats. Antibiotic sensitivity profiles have been used to distinguish relapsing or persisting UTI from reinfection by different clones of the same species. However, the accuracy with which antibiograms discriminate different urinary E coli clones in cats is uncertain. We studied 17 cystocentesis-derived UPEC isolates collected from 5 cats with stable CRF and multiple diagnoses of UTI. UTIs were classified as relapses versus persistent infections or reinfections using antibiograms determined by Kirby-Bauer discs and Etests. Subsequently, clonality of UPEC isolates was determined by pulsed-field gel electrophoresis (PFGE). A comparison of PFGE results with antibiograms indicated that antibiotic resistance patterns varied considerably within several individual E coli clones. Both antibiotic susceptibility tests differentiated between relapsing or persistent infections and reinfections with only 58% overall efficiency. Thus, antibiotic sensitivity profiles cannot be relied upon to distinguish between persisting or relapsing infections as compared to reinfections in cats with CRF and multiple diagnoses of E coli UTI. 相似文献
72.
KE Borer SR Bailey NJ Menzies-Gow PA Harris J Elliott 《Journal of animal science》2012,90(9):3003-3011
Identification of ponies (Equus caballus) at increased risk of pasture-associated laminitis would aid in the prevention of the disease. Insulin resistance has been associated with laminitis and could be used to identify susceptible individuals. Insulin resistance may be diagnosed by feeding supplementary water-soluble carbohydrate (WSC) and measuring blood glucose and insulin concentrations. The aim of this study was to assess the glycemic and insulinemic responses of 7 normal (NP) and 5 previously laminitic (PLP), mixed breed, native UK ponies fed glucose, fructose, and inulin [1 g/(kg(.)d) for 3 d] or no supplementary WSC (control) in spring and fall after a 7-d adaptation to a pasture or hay diet. Blood samples were taken for 12 h after feeding on each day, and baseline and peak concentrations and area under the curve (AUC) for glucose and insulin were recorded. Linear mixed models were used for statistical analysis. Differences between PLP and NP groups were most marked after glucose feeding with differences in peak glucose (P = 0.02) and peak insulin (P = 0.016) concentrations. Season and diet adaptation also affected results. Peak concentrations of glucose and insulin occurred 2 to 4 h after WSC feeding. Peak insulin concentration was greater and more variable in fall, particularly in PLP adapted to fall pasture. Baseline glucose and insulin concentrations varied between individuals and with season and diet adaptation but were not greater in PLP than NP. Insulin AUC was greater in PLP than NP after feeding both glucose and fructose (P = 0.017), but there were no differences between PLP and NP in glucose AUC. Glycemic and insulinemic changes were less (P ≤ 0.05) after feeding fructose than glucose, although differences between PLP and NP were still evident. Minimal changes in glucose and insulin concentrations occurred after inulin feeding. Measurement of peak insulin 2 h after feeding of a single dose of glucose (1 g/kg) may be a simple and practical way to aid identification of laminitis-prone ponies before the onset of clinical disease, particularly when ponies are adapted to eating fall pasture. 相似文献
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74.
The success of direct seeding, as a low-cost approach to forest restoration, varies with tree species and seed characteristics.
A system to predict which tree species are likely to be suitable for direct seeding would therefore be useful for improving
forest restoration projects. Therefore, this study aimed to determine the effects of seed traits on the success of direct
seeding to restore tropical forest in southern Thailand. Seeds of 19 indigenous lowland tropical forest tree species were
collected, from both the east and west sides of the Thailand peninsular and the following parameters measured: seed size,
shape, coat thickness and moisture content. Field trials were established to determine seed germination rates and to calculate
a “direct seeding suitability score” for each species, which combined seedling establishment and growth rates. Seed size,
shape and moisture content were associated with germination percentage. Species with higher seedling survival tended to have
large or intermediate-sized seeds, round or oval seeds and seeds with low or medium moisture content. Only seed coat thickness
was significantly correlated with the suitability score (r = 0.65, p < 0.01). Seed coat thickness >0.4 mm coincided with higher rank suitability score. These conditions successfully predicted
the success or failure of direct seeding for 15 out of 19 species tested. Eight tree species are recommended as suitable for
restoring lowland evergreen forest ecosystems in southern Thailand, by direct seeding. 相似文献
75.
Wellehan JF Pessier AP Archer LL Childress AL Jacobson ER Tesh RB 《Veterinary microbiology》2012,158(3-4):274-279
Rhabdoviruses infect a variety of hosts, including non-avian reptiles. Consensus PCR techniques were used to obtain partial RNA-dependent RNA polymerase gene sequence from five rhabdoviruses of South American lizards; Marco, Chaco, Timbo, Sena Madureira, and a rhabdovirus from a caiman lizard (Dracaena guianensis). The caiman lizard rhabdovirus formed inclusions in erythrocytes, which may be a route for infecting hematophagous insects. This is the first information on behavior of a rhabdovirus in squamates. We also obtained sequence from two rhabdoviruses of Australian lizards, confirming previous Charleville virus sequence and finding that, unlike a previous sequence report but in agreement with serologic reports, Almpiwar virus is clearly distinct from Charleville virus. Bayesian and maximum likelihood phylogenetic analysis revealed that most known rhabdoviruses of squamates cluster in the Almpiwar subgroup. The exception is Marco virus, which is found in the Hart Park group. 相似文献
76.
The aim of the study was to evaluate if thymidine kinase (TK) correlated with duration of first remission (DFR) or survival in dogs with lymphoma and if initial TK levels correlated with stage and substage; and also to assess if TK level at diagnosis correlated with immunophenotype. TK was assayed in 73 dogs with treatment naïve lymphoma, then again after treatment; 47% had an initial TK above the reference interval. Dogs with B‐cell lymphoma had higher initial TK activities than dogs with T‐cell lymphoma. TK levels were not higher in dogs with higher stage disease and TK activity prior to treatment was not associated with DFR or survival. Where TK was elevated at diagnosis, it fell into the reference range during remission. TK was normal in 53% dogs at diagnosis, which is higher than previously reported. Further studies are warranted to assess the utility of TK in dogs with lymphoma. 相似文献
77.
78.
R.E.W. Elliott B.V.Sc M.R.C.V.S. Dip.Bact. J.D. McNab B.V.Sc. 《New Zealand veterinary journal》2013,61(6):108-110
Extract This paper reports the diagnosis and spread of bovine viral diarrhoea (BVD) in cattle on the east coast of the North Island. The suspected origin, difficulty in diagnosis, and the spread are discussed from the practitioner's viewpoint. 相似文献
79.
80.