Control of citrus pathogens by protein extracts from Solanum tuberosum tubers     

I. F. Rodríguez  J. E. Sayago  S. Torres  I. C. Zampini  M. I. Isla  R. M. Ordóñez 《European journal of plant pathology / European Foundation for Plant Pathology》2015,141(3):585-595

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Metabolic responses of avocado plants to stress induced by Rosellinia necatrix analysed by fluorescence and thermal imaging     

Granum  Espen  Pérez-Bueno  María Luisa  Calderón  Claudia E.  Ramos  Cayo  de Vicente  Antonio  Cazorla  Francisco M.  Barón  Matilde 《European journal of plant pathology / European Foundation for Plant Pathology》2015,142(3):625-632

European Journal of Plant Pathology - One of the most important soilborne diseases affecting avocado (Persea americana Mill.) crops is white root rot, caused by the fungus Rosellinia necatrix. In...  相似文献   

Amaranth Peptides from Simulated Gastrointestinal Digestion: Antioxidant Activity Against Reactive Species     

Delgado  María C. Orsini  Galleano  Mónica  Añón  María C.  Tironi  Valeria A. 《Plant foods for human nutrition (Dordrecht, Netherlands)》2015,70(1):27-34

Plant Foods for Human Nutrition - We evaluated the capacity of simulated gastrointestinal digests or alcalase hydrolysates of protein isolates from amaranth to scavenge diverse physiologically...  相似文献   

Obtaining from Grape Pomace an Enzymatic Extract with Anti-inflammatory Properties     

Rodríguez-Morgado  B.  Candiracci  M.  Santa-María  C.  Revilla  E.  Gordillo  B.  Parrado  J.  Castaño  A. 《Plant foods for human nutrition (Dordrecht, Netherlands)》2015,70(1):42-49

Grape pomace, a winemaking industry by-product, is a rich source of bioactive dietary compounds. Using proteases we have developed an enzymatic process for obtaining a water-soluble extract (GP-EE) that contains biomolecules such as peptides, carbohydrates, lipids and polyphenols in soluble form. Of especial interest is its high polyphenol content (12 %), of which 77 % are flavonoids and 33 % are phenolic acids. The present study evaluates in vitro the potential anti-inflammatory effect of GP-EE by monitoring the expression of inflammatory molecules on N13 microglia cells stimulated with lipopolysaccharide (LPS). GP-EE decreases the mRNA levels of the inflammatory molecules studied. The molecules under study were as follows: inducible nitric oxide synthase (iNOS), tumor necrosis factor- α (TNF-α), interleukin-1β (IL-1β), the ionized calcium binding adaptor molecule-1(Iba-1) and the Toll like receptor-4 (TLR-4), as well as the iNOS protein level in LPS-stimulated microglia. Our findings suggest that, as a result of its ability to regulate excessive microglial activation, GP-EE possesses anti-inflammatory properties. Therefore, acting as a chemopreventive agent, it may be of therapeutic interest in neurodegenerative diseases involving neuroinflammation. We can, therefore, propose GP-EE as a useful natural extract and one that would be beneficial to apply in the field of functional foods.

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Dereplication and Chemotaxonomical Studies of Marine Algae of the Ochrophyta and Rhodophyta Phyla     

Robert Brklja?a  Emrehan Semih G?ker  Sylvia Urban 《Marine drugs》2015,13(5):2714-2731

Dereplication and chemotaxonomic studies of six marine algae of the Ochrophyta and one of the Rhodophyta phyla resulted in the detection of 22 separate compounds. All 16 secondary metabolites, including four new compounds (16–19), could be rapidly dereplicated using HPLC-NMR and HPLC-MS methodologies in conjunction with the MarinLit database. This study highlights the advantages of using NMR data (acquired via HPLC-NMR) for database searching and for the overall dereplication of natural products.  相似文献   

Tetrodotoxin,an Extremely Potent Marine Neurotoxin: Distribution,Toxicity, Origin and Therapeutical Uses     

Jorge Lago  Laura P. Rodríguez  Lucía Blanco  Juan Manuel Vieites  Ana G. Cabado 《Marine drugs》2015,13(10):6384-6406

Tetrodotoxin (TTX) is a potent neurotoxin responsible for many human intoxications and fatalities each year. The origin of TTX is unknown, but in the pufferfish, it seems to be produced by endosymbiotic bacteria that often seem to be passed down the food chain. The ingestion of contaminated pufferfish, considered the most delicious fish in Japan, is the usual route of toxicity. This neurotoxin, reported as a threat to human health in Asian countries, has spread to the Pacific and Mediterranean, due to the increase of temperature waters worldwide. TTX, for which there is no known antidote, inhibits sodium channel producing heart failure in many cases and consequently death. In Japan, a regulatory limit of 2 mg eq TTX/kg was established, although the restaurant preparation of “fugu” is strictly controlled by law and only chefs qualified are allowed to prepare the fish. Due to its paralysis effect, this neurotoxin could be used in the medical field as an analgesic to treat some cancer pains.  相似文献   

Non‐invasive fast real‐time PCR assay for detection of the enteric parasite Enteromyxum scophthalmi in cultured turbot (Scophthalmus maximus L.)          下载免费PDF全文

Mercedes Alonso  Fátima C Lago  María Gómez‐Reino  Jacobo Fernández Casal  Iris Martín Varela  Juan M Vieites  Montserrat Espiñeira 《Aquaculture Research》2015,46(9):2104-2115

Enteromyxum scophthalmi is a myxozoan parasite that causes severe parasitic diseases in cultured turbot affecting mainly the intestine of the host. It is characterized by producing acute enteritis, starvation and eventually death. Current diagnosis of E. scopthalmi use traditional techniques, based on the identification of the morphology of the parasite. These techniques take extended time to be carried out and do not favour the adoption of control measure at turbot farms and require the sacrifice of fish. This study develops a fast real‐time PCR molecular tool for the detection of E. scophthalmi in infected farmed turbot. This methodology is applicable for routine controls on the farm at every stage of the parasite infection. Results of the study demonstrate the robustness, specificity, efficiency and reliability of the technique. In addition, this study also provides a non‐invasive procedure of sampling through swaps. This allows control, prevention and diagnosis of the parasite infection at turbot farms while maintaining the welfare of the cultivated fish and avoiding sacrifice of the fish sampled.  相似文献   

Evaluation of frozen Umbrella‐stage Artemia as first animal live food for Litopenaeus vannamei (Boone) larvae          下载免费PDF全文

María de Lourdes Cobo  Roeland Wouters  Mathieu Wille  Stanislaus Sonnenholzner  Patrick Sorgeloos 《Aquaculture Research》2015,46(9):2166-2173

An alternative larval shrimp feeding regime, in which umbrella‐stage Artemia were constituting the first zooplankton source was evaluated in the culture of Litopenaeus vannamei. In a preliminary experiment, umbrella‐stage Artemia were fed to larvae from zoea 2 (Z2) to mysis 2 (M2) stages to identify the larval stage at which raptorial feeding starts and to determine daily feeding rates. The following experiment evaluated the performance of two feeding regimen that differed during the late zoea/early mysis stages: a control treatment with frozen Artemia nauplii (FAN), and a treatment with frozen umbrella‐stage Artemia (FUA). The ingestion rate of umbrella‐stage Artemia increased from nine umbrella per larvae day^?1 at Z2 stage to 21 umbrella per larvae day^?1 at M2. A steep increase in ingestion and dry weight from Z3 to M2 suggests a shift to a raptorial feeding mode at the M1 stage. Treatment FUA exhibited a significantly higher larval stage index (P < 0.05) during the period that zoea larvae metamorphosed to the mysis stage, and a higher final biomass, compared with treatment FAN. Based on these results and on practical considerations, a feeding regime starting with umbrella‐stage Artemia from Z2 sub‐stage can be recommended for L. vannamei larvae rearing.  相似文献   

A continuous diphasic model for prediction of survival of cultivated populations when affected by disease: the case of shrimp and white spot disease          下载免费PDF全文

Javier M J Ruiz‐Velazco  Alfredo Hernández‐Llamas  Victor M Gomez‐Muñoz 《Aquaculture Research》2015,46(12):3020-3027

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Chestnut Blight Control by Agrochemicals in Castanea sativa under Managed Conditions     

Trapiello  Estefanía  González-Varela  Germán  González  Ana J 《植物病害和植物保护杂志》2015,122(3):120-124

Journal of Plant Diseases and Protection - This research was conducted with the aim of evaluating the in vivo effectiveness of three agrochemical products to control chestnut blight disease in...  相似文献