The influence of Pueraria mirifica herb containing phytoestrogens on the urinary gonadotropin and estradiol levels in aged menopausal monkeys     

Hataitip TRISOMBOON  Suchinda MALAIVIJITNOND  Wichai CHERDSHEWASART  Gen WATANABE  Kazuyoshi TAYA 《Animal Science Journal》2007,78(4):378-386

We investigated a non‐invasive method of specimen collection for determining the changes of reproductive hormones in aged menopausal monkeys after a long‐term feeding of the Thai herb Pueraria mirifica (PM) containing phytoestrogens. Three groups of aged menopausal monkeys (n = three in each group) were fed daily with 10, 100, or 1000 mg of PM for a 90 day treatment period, and fed with distilled water for 30 and 60 days of the pre‐ and post‐treatment periods, respectively. Urine samples were collected for 14 h daily every 5 days and assayed for follicle stimulating hormone (FSH), luteinizing hormone (LH) and estradiol levels. The result showed that monkeys fed with PM 10, PM 100, and PM 1000 had a decrease in urinary FSH levels during the treatment period, followed by a rebound increase during the post‐treatment period. Urinary estradiol levels tended to decrease and fluctuated between 4.28 and 266.71, 2.85–42.27, and 6.24–203.50% of the pre‐treatment levels in those three groups, respectively. Decreases in urinary LH levels could not be observed in all the three groups. These results suggest that FSH could be a candidate marker to detect the estrogenic effects of phytoestrogens in aged menopausal monkeys when changes of urinary hormones need to be used as an indicator.  相似文献   

Reduced Efficacy of Ivermectin Treatments in Gastrointestinal Nematode Infections of Grazing Cattle in Japan     

Noboru KUDO  Toshiro YOSHIOKA  Yasushi WATANABE  Yuki TERAZONO  Shino TAKENOUCHI  Takuto DONOMOTO  Kensuke NAKAJIMA  Kaori HITOSUGI  Ryusuke TSUKADA  Hiromi IKADAI  Takashi OYAMADA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(11):1487-1491

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Transgenic Pigs with Pancreas-specific Expression of Green Fluorescent Protein     

Hitomi MATSUNARI  Toshihiro KOBAYASHI  Masahito WATANABE  Kazuhiro UMEYAMA  Kazuaki NAKANO  Takahiro KANAI  Taisuke MATSUDA  Masaki NAGAYA  Manami HARA  Hiromitsu NAKAUCHI  Hiroshi NAGASHIMA 《The Journal of reproduction and development》2014,60(3):230-237

The development and regeneration of the pancreas is of considerable interest because of the role of these processes in pancreatic diseases, such as diabetes. Here, we sought to develop a large animal model in which the pancreatic cell lineage could be tracked. The pancreatic and duodenal homeobox-1 (Pdx1) gene promoter was conjugated to Venus, a green fluorescent protein, and introduced into 370 in vitro-matured porcine oocytes by intracytoplasmic sperm injection-mediated gene transfer. These oocytes were transferred into four recipient gilts, all of which became pregnant. Three gilts were sacrificed at 47–65 days of gestation, and the fourth was allowed to farrow. Seven of 16 fetuses obtained were transgenic (Tg) and exhibited pancreas-specific green fluorescence. The fourth recipient gilt produced a litter of six piglets, two of which were Tg. The founder Tg offspring matured normally and produced healthy first-generation (G1) progeny. A postweaning autopsy of four 27-day-old G1 Tg piglets confirmed the pancreas-specific Venus expression. Immunostaining of the pancreatic tissue indicated the transgene was expressed in β-cells. Pancreatic islets from Tg pigs were transplanted under the renal capsules of NOD/SCID mice and expressed fluorescence up to one month after transplantation. Tg G1 pigs developed normally and had blood glucose levels within the normal range. Insulin levels before and after sexual maturity were within normal ranges, as were other blood biochemistry parameters, indicating that pancreatic function was normal. We conclude that Pdx1-Venus Tg pigs represent a large animal model suitable for research on pancreatic development/regeneration and diabetes.  相似文献   

Notch2 Transduction by Feline Leukemia Virus in a Naturally Infected Cat     

Shinya WATANABE  Jumpei ITO  Takuya BABA  Takahiro HIRATSUKA  Kyohei KUSE  Haruyo OCHI  Yukari ANAI  Masaharu HISASUE  Hajime TSUJIMOTO  Kazuo NISHIGAKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(4):553-557

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Distribution of Glucagon-Like Peptide (GLP)-2-Immunoreactive Cells in the Chicken Small Intestine: Antigen Retrieval Immunohistochemistry     

Mohammad M. MONIR  Kohzy HIRAMATSU  Kei NISHIMURA  Chihiro TAKEMOTO  Takafumi WATANABE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(4):565-568

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Differentially methylated CpG sites related to fertility in Japanese Black bull spermatozoa: epigenetic biomarker candidates to predict sire conception rate     

Kumiko TAKEDA  Eiji KOBAYASHI  Kazuko OGATA  Akira IMAI  Shinya SATO  Hiromichi ADACHI  Yoichiro HOSHINO  Kagetomo NISHINO  Masahiro INOUE  Masahiro KANEDA  Shinya WATANABE 《The Journal of reproduction and development》2021,67(2):99

For semen suppliers, predicting the low fertility of service bull candidates before artificial insemination would help prevent economic loss; however, predicting bull fertility through in vitro assessment of semen is yet to be established. In the present study, we focused on the methylated CpG sites of sperm nuclear DNA and examined methylation levels to screen new biomarkers for predicting bull fertility. In frozen-thawed semen samples collected from Japanese Black bulls, for which the sire conception rate (SCR) was recorded, the methylation level of each CpG site was analyzed using human methylation microarray. According to regression analysis, 143 CpG sites related to SCR were significantly differentially methylated. Whole genome bisulfite sequence data were obtained from three semen samples and the differentially methylated regions (DMRs) that included the target CpG sites selected by human methylation microarray were confirmed. Using combined bisulfite restriction analysis, fertility-related methylation changes were detected in 10 DMRs. With the exception of one DMR, the methylation levels of these DMRs were significantly different between groups with high fertility (> 50%) and low fertility (< 40%). From multiple regression analysis of methylation levels and SCR, three DMRs were selected that could effectively predict bull fertility. We suggest that these fertility-related differences in spermatozoal methylation levels could be new epigenetic biomarkers for predicting bull fertility.  相似文献   

⁵²Mn translocation in barley monitored using a positron-emitting tracer imaging system     

Takashi TSUKAMOTO  Hiromi NAKANISHI  Shoichiro KIYOMIYA  Satoshi WATANABE  Shinpei MATSUHASHI  Naoko K. NISHIZAWA  Satoshi MORI 《Soil Science and Plant Nutrition》2006,52(6):717-725

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Late seeding of soybean after rice transplanting period using variety "Yukihomare" in central area of Hokkaido     

Jiro WATANABE  Yasuo OHSHITA  Hiroyuki TSUJI  Tsuneo NIHEI  Kenji KIMIWADA  Kenichiro HONDA 《Soil Science and Plant Nutrition》2006,52(4):577-577

(Jpn. J. Soil Sci.Plant Nutr., 77, 293–298, 2006)
"Kitanokaori" is a new variety of wheat for bread use bred at the National Agricultural Research Center for Hokkaido Region. The grain protein content of wheat for bread use should be higher than 120 g kg−1. Much nitrogen application is necessary to obtain high grain protein content. Therefore, it is necessary to determine the optimum amount of nitrogen to obtain the required protein content and to prevent nitrogen from remaining in the soil. The nutrition diagnosis using leaf color was investigated to predict the need and the amount of top-dressing. Field experiments were conducted for four years with nitrogen treatments in Andosol, which has moderate nitrogen fertility, and in Histosol, which is a fertile soil. The leaf color was measured using a chlorophyll meter SPAD502 (CM value) at the middle part of the leaf, avoiding the center rib. The colors of the 10·15 uppermost second leaves were measured in one plot and averaged.
A close relation was found between leaf color at the full heading stage and grain protein content at harvest. Leaf color at the full heading stage is therefore a good index to control the protein content. Considering the effect of top-dressing at the full heading stage in each CM value, the diagnosis criterion was decided. When the CM value is over 52 at the full heading stage, more nitrogen application is not needed. When the CM value is 50·52, 30 kg N ha−1 of top-dressing at the full heading stage is needed, and when the CM value is 45·50, 60 kg N ha−1 of top-dressing is needed to obtain a grain protein content of more than 120 g kg−1.  相似文献   

Vertical distribution of chloroform-extractable green fraction in several Andosol and Cambisol profiles     

Takayuki KOBAYASHI  Makiko WATANABE  Hiroyuki OHTA  Nobuhide FUJITAKE 《Soil Science and Plant Nutrition》2006,52(3):274-283

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Phosphorus retention efficiency in rainbow trout fed diets with low fish meal and alternative protein ingredients     

Adrián  HERNÁNDEZ  Shuichi  SATOH  Viswanath  KIRON  Takeshi  WATANABE 《Fisheries Science》2004,70(4):580-586

ABSTRACT:   Two experiments were conducted to evaluate feed quality and body phosphorus (P) and nitrogen (N) retention by rainbow trout fed test diets with low amounts of fish meal content and alternative low-P protein sources. A fish meal (FM) diet was used as control. Fish weighing 2.0 g and 134.7 g on average were reared with the experimental diets for 30 weeks and 15 weeks, respectively. The experimental diets had a good growth rate and feed utilization. In the first experiment the P retention was higher in the group of fish fed test diets (56 and 69%) compared to the FM-based diet (36%); N retention rates were similar for all groups. In the second experiment, fish were fed the test diet that had the best P retention in the earlier experiment. The P retention was lower than the values obtained in the first experiment (smaller fish), but still higher in the group of fish fed the low FM diet in comparison with the control group (36.0% and 22.2%, respectively). This represents a P loading into the water of 5.9 kg/t and 12.8 kg/t production for the test and the control diet, respectively. Therefore, low-P-loading diets for rainbow trout can be developed through the appropriate combination of alternative protein sources.  相似文献