Immunogenicity of bovine parainfluenza type 3 virus proteins encapsulated in nanoparticle vaccines,following intranasal administration to mice   总被引：2，自引：0，他引：2  

Shephard MJ  Todd D  Adair BM  Po AL  Mackie DP  Scott EM 《Research in veterinary science》2003,74(2):187-190

Stimulation of long lasting, protective immunity to respiratory viruses is often difficult to achieve with conventional respiratory vaccines. Polymeric nanoparticles, incorporating viral proteins have been shown to offer sustained release of antigen, with consequent prolongued stimulation of the respiratory immune system. In this paper the efficacy of two nanoparticle vaccines (poly-lactide-co-glycolide, PLGA; polymethylmethacrylate, PMMA), incorporating proteins of bovine parainfluenza type 3 virus (BPI-3) was investigated. As a preliminary to experiments in calves, it was considered essential to demonstrate immunogenicity of the experimental vaccine in mice. Mice immunised with PLGA nanoparticles, containing BPI-3 proteins, developed higher levels of virus-specific antibody than mice immunised with the PMMA vaccine or with soluble viral proteins alone. Immunoblotting using serum from the vaccinated mice, demonstrated strong reactions against the major BPI-3 proteins.  相似文献   

Characterization of monoclonal antibodies against avian reovirus S1133 protein sigmaA synthesized in Escherichia coli     

Pai WC  Shien JH  Liu HJ  Lee LH 《Veterinary microbiology》2003,91(4):309-323

Monoclonal antibodies (MAbs) were prepared against avian reovirus S1133 protein sigmaA (esigmaA) synthesized in Escherichia coli. MAbs were characterized and used to develop a diagnostic test. Ten MAbs were selected for competitive binding assay following coupling with horseradish peroxidase. The results indicated that these MAbs delineated two epitopes I and II of esigmaA. An immuno-dot binding assay was used to detect the effect of denaturation on antibody recognition of the epitopes. All MAbs bound to esigmaA in its native form. After denaturation by boiling in SDS and 2-mercaptoethanol, the binding of MAbs recognizing epitope I was fully abolished. However, the reactivity of MAbs recognizing epitope II was not affected. MAbs 31 and 32, recognizing epitopes I and II, respectively, were selected for the cross-reactivity to heterologous reovirus strains. The results suggest that the two epitopes are highly conserved among these virus strains. A MAb capture enzyme-linked immunosorbent assay (ELISA) procedure was developed using MAbs 32 and 31 to detect reovirus protein sigmaA in samples from tendon tissues of infected bird and chicken embryo fibroblast (CEF) cell cultures. Avian reovirus sigmaA antigens in tendon specimens were detected from the inoculated birds as early as 2 days post-inoculation (PI), approximated a peak at 7 days PI, and maintained this until 16 days PI, then decreased gradually. A clear difference in absorbance values between the tendon samples of the avian reovirus- and mock-infected birds is obtained. Positive results were also obtained from avian reovirus-infected CEF and from the tendon tissues of naturally infected broilers. These results indicated that the MAb capture ELISA is a useful methods for the detection of avian reovirus from chickens suspected to have avian reovirus infections.  相似文献   

茶秆竹高生长模型的比较及组合选择   总被引：2，自引：1，他引：1  

代全林  陈存及  肖书平  陈东阳  董建文 《福建林学院学报》2002,22(2):129-132

通过对 5种 S增长模型的比较及组合选择 ,结果表明 :S增长模型都具有单调性、渐近性和拐点 ,此特征能客观地反映诸多自然和经济规律 .通过一定的加权组合 ,用多个增长模型来反映 S变化过程 ,可以提高拟合的精度 ,多个模型的组合要优于单个模型 .用加权模型可以很好地反映茶秆竹的高生长过程 ,在实际生产中可选择 yp2 或 yp 3 组合  相似文献   

纳罗克非洲狗尾草种子生产技术研究概况     

罗泉  邓菊芬  尹俊 《四川草原》2007,(2)

从施肥、刈割、植物生长调节剂、收获时间等方面论述了纳罗克非洲狗尾草种子生产的主要环节,提出了增加种子产量的关键措施。  相似文献   

溶菌酶在食品工业中的应用   总被引：2，自引：0，他引：2  

谷绒  车振明  万国福 《乳业科学与技术》2006,28(6):264-266

本文综述了溶菌酶的基本性质，着重讨论了溶菌酶抑制的微生物种类、机理，系统的阐述了溶菌酶的制备和在食品工业生产过程中应用的进展情况。  相似文献   

蛋白质饲料木聚糖含量及其体外酶解效果研究   总被引：6，自引：0，他引：6  

张慎忠  张克英  丁雪梅  谭权 《动物营养学报》2007,19(6):719-724

本文通过体外酶解试验,考察木聚糖酶对豆粕、菜籽粕和棉籽粕的总木聚糖和水溶性木聚糖的降解效果,试图建立木聚糖酶与不同蛋白质饲料种类的定量关系.试验结果表明:①不同蛋白质饲料的总木聚糖和水溶性木聚糖的含量不同,豆粕、菜籽粕和棉籽粕的总木聚糖含量分别为5.62%、6.56%和8.20%;水溶性木聚糖含量分别为0.35%、0.97%和0.84%;②不同来源的3种蛋白质饲料的总木聚糖和水溶性木聚糖含量变异较小.在适宜的水环境中,菜籽粕的水溶性木聚糖含量显著增加,豆粕和棉籽粕的水溶性木聚糖变化不大;③木聚糖酶对3种蛋白质饲料的总木聚糖均有显著降解作用,对水溶性木聚糖有一定的降解作用,其中对菜籽粕的总木聚糖和水溶性木聚糖的降解作用均较大;以总木聚糖酶为标识,结合考虑成本,豆粕、菜籽粕、棉籽粕的适宜添加木聚糖酶水平分别为1 000、1 000和1 500 U/kg.  相似文献   

联合国探雷犬使用指南     

张跃骏  万九生  杨玉明 《中国工作犬业》2006,(12):50-50

(IMAS09.40,2003-01-01第1次出版)前言国际人道主义排雷程序标准是于1996年7月在丹麦的一个专题研讨会上提出的,会上提出了排除各种地雷的标准,并通过研讨修订后颁布了标准。至同年年底,在丹麦通过的标准最后发展成由联合国领导工作组颁布的国际人道主义排雷操作标准。此标准由  相似文献   

双重排水条件下控制措施对银南灌区水稻田水盐关系的影响分析   总被引：4，自引：0，他引：4  

贾忠华  罗纨  方树星  景卫华  WANG Liang 《干旱区资源与环境》2006,20(5):213-216

针对宁夏银南灌区目前存在的排水过量问题,研究者提出了利用控制排水措施,在适当时段减少排水量的建议。然而,灌区在农沟以及更深层的支、干沟及黄河(低地)的影响下,受着深层排水和浅层排水的双重作用。其中深层排水一般不会受到田间措施的影响,它的存在“淡化或中和”了由于浅层排水的减少引起的田间含盐量的增加。本文在对双重排水情况下的水盐运动进行概化的基础上,建立了基于特定地区排水特性的“浅层排水与深层排水比-浅层排水控制率-排水含盐量增加率”的关系。最后结合根据地下水洗淋的临界情况,确定了达到这一目标控制指标以及浅层地下水控制率。结果显示,对于研究的典型情况,当地下水的盐分含量达到临界的1.62 g/l时,可以将1m深的农沟控制至30cm深;这时的浅层地下水仅为现状的14%,即从731mm减少到了102mm。  相似文献   

柑桔粉虱发生为害特点及防治对策     

曾泉  王长青  施昌年 《湖北植保》2006,(5):6-7

柑桔粉虱(Dialeurodes citri Ashmead)又名白粉虱、桔黄粉虱、通草粉虱等,近年来在枝江市柑桔产区为害日趋严重,年发生面积5 800hm2,占全市柑桔种植面积的80%以上.该虫主要以成、幼虫在叶背吸取汁液,并分泌蜜露诱发煤烟病,使枝、叶、果变黑,阻碍叶片光合作用,造成树势衰弱、产量下降、品质变劣.为害严重时,新梢纤弱不充实,抽生数量少,翌年极少开花结果.为有效控制其为害,我们自2002年开始对其发生为害特点、偏重发生原因及防治对策进行了调查研究.  相似文献   

Canine model of ischemic stroke with permanent middle cerebral artery occlusion: clinical and histopathological findings     

Byeong-Teck Kang  Jong-Hwan Lee  Dong-In Jung  Chul Park  Su-Hyun Gu  Hyo-Won Jeon  Dong-Pyo Jang  Chae-Young Lim  Fu-Shi Quan  Young-Bo Kim  Zang-Hee Cho  Eung-Je Woo  Hee-Myung Park 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(4):369-376

The aim of the present study was to assess the clinical and histopathological findings in a canine model of ischemic stroke. Cerebral ischemic stroke was induced by middle cerebral artery occlusion in four healthy beagle dogs using silicone plugs. They showed neurological signs of forebrain dysfunction such as reduced responsiveness, head turning, circling, postural reaction deficits, perceptual deficits, and hemianopsia. These signs gradually regressed within 4 weeks without therapy. On magnetic resonance imaging, T2 hyperintensity and T1 hypointensity were found in the cerebral cortex and basal ganglia. These lesions were well-defined and sharply demarcated from adjacent brain parenchyma with a homogenous appearance. No abnormalities of the cerebrospinal fluid were observed. At necropsy, atrophic and necrotic lesions were observed in the cerebral cortex. The cerebral cortex, basal ganglia, and thalamus were partially unstained with triphenyl-tetrazolium chloride. Histopathologically, typical features of infarction were identified in cortical and thalamic lesions. This study demonstrates that our canine model resembles the conditions of real stroke patients.  相似文献