The use of the toxin binding inhibition test for potency testing of Clostridium novyi type B alpha toxoid vaccines     

Luick K  Gottschaldt J  Schulze F  Werner E  Erler W  Borrmann E 《DTW. Deutsche tier?rztliche Wochenschrift》2002,109(4):178-182

The toxin binding inhibition test (ToBI) were developed for potency testing of C. novyi type B alpha toxoid containing veterinary vaccines to replace the currently used toxin neutralisation test in mice (TNT). The antitoxin titres of rabbit sera (AN-, HV- and SP sera) were determined with ToBI using the international reference serum with known antitoxin titre. In order to show the validity of the methods, the results were compared with those of the manufacturers/regulatory authorities and correlation coefficients were calculated. The correlation coefficients were r = 0.93 (AN sera), r = 0.73 (HV sera) and r = 0.85 (SP sera). All correlations were statistically significant. The specificity of the methods could be proved using heterologous antisera. The results of the ToBI were reproducible. Thus, the ToBI offers a suitable in vitro method for the determination of the antitoxin titre of rabbit antisera as an alternative to the toxin neutralisation in mice for potency testing of vaccines containing C. novyi type B alpha toxoid.  相似文献   

Immune-mediated diseases of the dog and cat. IV. Therapy and immunodiagnosis   总被引：1，自引：0，他引：1  

N T Gorman  L L Werner 《The British veterinary journal》1986,142(6):498-505

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Tumor disease and mortality without lymphomas following infection by Marek's disease virus in highly and slightly susceptible chickens     

J Beyer  O Werner  U Schmidt 《Archiv fuer experimentelle veterinaermedizin》1987,41(6):857-860

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A phagocytosis test for the evaluation of the local immunity status during endometritis therapy with special regard to the use of uterofertil in cattle     

K Strube  R Hühn  W Busch  E Werner 《DTW. Deutsche tier?rztliche Wochenschrift》1991,98(6):230-234

After the influence of organic acids or peroxide acids with low concentration upon mucous membranes results a higher activity of the topical defence-situation. The application of a 0.2 per cent solution of peroxyethane acid (drug Uterofertil) into the uterus for endometritis-therapy directs an increase of phagocytosis-activity not only topical but also in the peripheric circulation of blood. The phagocytosis-activity was measured with a test by granulocytes. Other drugs were also tested, which will needed by endometritis-treatment of cattle.  相似文献   

Confirmation of a 4BL/5RL Wheat-Rye Chromosome Translocation Line in the Wheat Cultivar ''Viking'' Showing High Copper Efficiency     

R. Schlegel    T. Werner  E. Hülgenhof 《Plant Breeding》1991,107(3):226-234

Populations of 64 taxa of Trifolium were assessed for nitrogen fixation and dry matter production with and without added mineral nitrogen. The number of rhizobia per gram of compost capable of inducing nodulation was tested in 7 species and ranged from 3 to 114, showing interspecific variation for nodulation with indigenous rhizobia. In a sample of 8 species, nitrogen fixation was assessed by acetylene reduction and nodule scoring. There was a good correlation (84 %, P ≤ 0.01) between the two methods, and therefore nodule score was used as a measure of nitrogen fixation in the main assessment. There were large differences between populations in terms of shoot and root dry weight, the shoot: root ratio and nodule score. Added nitrate had a significant effect on all characters, increasing shoot dry weight and the shoot: root ratio and decreasing root dry weight and the nodule score. Results showed the importance of assessing legume germplasm with and without added nitrate in locations where suitable rhizobia for the nodulation of exotic species might not be present.  相似文献   

Procedure for assessing the biologic suitability of dimethyl sulfoxide as a cryoprotective agent in preserving virus-infected primary poultry cells     

H Pick  P Werner  W Steinmann 《Archiv fuer experimentelle veterinaermedizin》1987,41(6):866-868

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Metabolism and fate of triazophos in rats     

Rudolf Bock  Werner Thier 《Pest management science》1976,7(3):307-314

The excretion patterns and tissue residues were determined after single and repeated oral dosing of rats with triazophos-¹⁴C Within 4 days after a single oral dose 76.3 % of the ¹⁴C was excreted in the urine and 21.0% in the faeces. After daily application for 12 days 69.5–83.4% of the label was eliminated in urine and 30.9–18.1 % in the faeces. Following prolonged application, however, elimination is distinctly slower. Distribution of radioactive residues in organs and tissue in both test series showed no appreciable or critical concentrations of radioactivity, with the exception of the gastrointestinal tract (contents and walls). Unchanged triazophos and l-phenyl-1,2,4-triazol-3-ol-3-¹⁴C were excreted in the faeces. Renewed release of other metabolites into the gastrointestinal tract apparently does not take place. The following metabolites are detected in the urine: urea-¹⁴C (approx. 85% of the radioactivity excreted with the urine); and three compounds as conjugates with glucuronic acid, i.e. 1-phenyl-l,2,4-triazol-3-ol-3-¹⁴C (approx. 3%), l-phenylsemicarbazide-3-¹⁴C (approx. 5%), and semicarbazide-¹⁴C (approx. 5%). Two further metabolites, so far unidentified, occurred in small quantities.  相似文献   

Toxicological prevention in the transition to industrialized production in socialist agriculture     

W Laue  S Krüger  W Netsch  E Werner 《Monatshefte für Veterin?rmedizin》1972,27(22):850-858

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灵芝中肿瘤细胞凋亡诱导剂的研究     

张劲松  唐庆九  REUTTER Werner  樊华 《食用菌学报》2006,13(2):15-28

灵芝水提物（LZ）对8种肿瘤细胞增殖的抑制作用具有剂量依赖关系.通过透析和离子交换凝胶过滤层析方法从LZ中分离出活性组分LZ-DN-2-2和LZ-DW-2-a-3.LZ、LZ-DN-2-2 和LZ-DW-2-a-3可剂量依赖地诱导SW620细胞凋亡,使细胞增殖周期停滞于G0期.  相似文献   

THE CORRELATION OF CANINE PATELLAR LUXATION AND THE ANTEVERSION ANGLE AS MEASURED USING MAGNETIC RESONANCE IMAGES     

Stephan  Kaiser  DVM  MS  Daniel  Cornely  MD  Werner  Colder  MD  PhD  Michael T.  Garner  BA  CVM  K.-J. Wolf  MD  PhD  Helmut  Waibl  DVM  PhD  Leo  Brunnberg  DVM  PhD 《Veterinary radiology & ultrasound》2001,42(2):113-118

Measurements from magnetic resonance (MR) images can be used to examine the anteversion angle (AT-angle) and its influence on the lateromedial or mediolateral luxating forces on the patella. The AT-angle of the femoral neck was measured with the aid of MR imaging in 45 pelvic limbs without patellar instability, in 33 limbs with patellar luxation and in 6 limbs with rupture of the cranial cruciate ligament. The limbs with medial patellar luxation were divided into three groups based on clinical examination. The mean (range) AT-angle was 7.6 degrees (0 degrees to 24 degrees) in the "normal" group, 8.6 degrees (-10 degrees to 29 degrees) in the group "grade II," and -0.4 degrees in the group "grade III" (-28 degrees to 12 degrees). A mean (range) AT-angle of 4.8 (-4 degrees to 11 degrees) was measured in the pelvic limbs with rupture of the cranial cruciate ligament. Compared to literature that described AT-angles based on radiographs of normal limbs, reduced AT-angles were found in this study due to different lines of measurement of the femoral neck. This study documents that the AT-angle of the femoral neck does not influence patellar instability. This study also demonstrates that MR images can be used to make exact measurements of the canine AT-angle that represent the true anatomy of the femoral neck.  相似文献