Procedure for assessing the biologic suitability of dimethyl sulfoxide as a cryoprotective agent in preserving virus-infected primary poultry cells     

H Pick  P Werner  W Steinmann 《Archiv fuer experimentelle veterinaermedizin》1987,41(6):866-868

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Metabolism and fate of triazophos in rats     

Rudolf Bock  Werner Thier 《Pest management science》1976,7(3):307-314

The excretion patterns and tissue residues were determined after single and repeated oral dosing of rats with triazophos-¹⁴C Within 4 days after a single oral dose 76.3 % of the ¹⁴C was excreted in the urine and 21.0% in the faeces. After daily application for 12 days 69.5–83.4% of the label was eliminated in urine and 30.9–18.1 % in the faeces. Following prolonged application, however, elimination is distinctly slower. Distribution of radioactive residues in organs and tissue in both test series showed no appreciable or critical concentrations of radioactivity, with the exception of the gastrointestinal tract (contents and walls). Unchanged triazophos and l-phenyl-1,2,4-triazol-3-ol-3-¹⁴C were excreted in the faeces. Renewed release of other metabolites into the gastrointestinal tract apparently does not take place. The following metabolites are detected in the urine: urea-¹⁴C (approx. 85% of the radioactivity excreted with the urine); and three compounds as conjugates with glucuronic acid, i.e. 1-phenyl-l,2,4-triazol-3-ol-3-¹⁴C (approx. 3%), l-phenylsemicarbazide-3-¹⁴C (approx. 5%), and semicarbazide-¹⁴C (approx. 5%). Two further metabolites, so far unidentified, occurred in small quantities.  相似文献   

Toxicological prevention in the transition to industrialized production in socialist agriculture     

W Laue  S Krüger  W Netsch  E Werner 《Monatshefte für Veterin?rmedizin》1972,27(22):850-858

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灵芝中肿瘤细胞凋亡诱导剂的研究     

张劲松  唐庆九  REUTTER Werner  樊华 《食用菌学报》2006,13(2):15-28

灵芝水提物（LZ）对8种肿瘤细胞增殖的抑制作用具有剂量依赖关系.通过透析和离子交换凝胶过滤层析方法从LZ中分离出活性组分LZ-DN-2-2和LZ-DW-2-a-3.LZ、LZ-DN-2-2 和LZ-DW-2-a-3可剂量依赖地诱导SW620细胞凋亡,使细胞增殖周期停滞于G0期.  相似文献   

THE CORRELATION OF CANINE PATELLAR LUXATION AND THE ANTEVERSION ANGLE AS MEASURED USING MAGNETIC RESONANCE IMAGES     

Stephan  Kaiser  DVM  MS  Daniel  Cornely  MD  Werner  Colder  MD  PhD  Michael T.  Garner  BA  CVM  K.-J. Wolf  MD  PhD  Helmut  Waibl  DVM  PhD  Leo  Brunnberg  DVM  PhD 《Veterinary radiology & ultrasound》2001,42(2):113-118

Measurements from magnetic resonance (MR) images can be used to examine the anteversion angle (AT-angle) and its influence on the lateromedial or mediolateral luxating forces on the patella. The AT-angle of the femoral neck was measured with the aid of MR imaging in 45 pelvic limbs without patellar instability, in 33 limbs with patellar luxation and in 6 limbs with rupture of the cranial cruciate ligament. The limbs with medial patellar luxation were divided into three groups based on clinical examination. The mean (range) AT-angle was 7.6 degrees (0 degrees to 24 degrees) in the "normal" group, 8.6 degrees (-10 degrees to 29 degrees) in the group "grade II," and -0.4 degrees in the group "grade III" (-28 degrees to 12 degrees). A mean (range) AT-angle of 4.8 (-4 degrees to 11 degrees) was measured in the pelvic limbs with rupture of the cranial cruciate ligament. Compared to literature that described AT-angles based on radiographs of normal limbs, reduced AT-angles were found in this study due to different lines of measurement of the femoral neck. This study documents that the AT-angle of the femoral neck does not influence patellar instability. This study also demonstrates that MR images can be used to make exact measurements of the canine AT-angle that represent the true anatomy of the femoral neck.  相似文献   

Agrochemical leaching reduction in biochar-amended tropical soils of Belize     

Gerardo O. Aldana  Charles Hazlerigg  Elisa Lopez-Capel  David Werner 《European Journal of Soil Science》2021,72(3):1243-1255

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Establishment success in a forest biodiversity and ecosystem functioning experiment in subtropical China (BEF-China)     

Xuefei Yang  Jürgen Bauhus  Sabine Both  Teng Fang  Werner Härdtle  Wenzel Kröber  Keping Ma  Karin Nadrowski  Kequan Pei  Michael Scherer-Lorenzen  Thomas Scholten  Gunnar Seidler  Bernhard Schmid  Goddert von Oheimb  Helge Bruelheide 《European Journal of Forest Research》2013,132(4):593-606

Experimental forest plantations to study biodiversity–ecosystem functioning (BEF) relationships have recently been established in different regions of the world, but subtropical biomes have not been covered so far. Here, we report about the initial survivorship of 26 tree species in the first such experiment in subtropical China. In the context of the joint Sino–German–Swiss Research Unit “BEF-China,” 271 experimental forest plots were established using 24 naturally occurring tree species and two native commercial conifers. Based on the survival inventories carried out in November 2009 and June 2010, the overall survival rate was 87 % after the first 14 months. Generalized mixed-effects models showed that survival rates of seedlings were significantly affected by species richness, the species’ leaf habit (deciduous or evergreen), species identity, planting date, and altitude. In the first survey, seedling establishment success decreased with increasing richness levels, a tendency that disappeared in the second survey after replanting. Though evergreen species performed less well than deciduous species with establishment rates of 84 versus 93 % in the second survey, their planting success exceeded the general expectation for subtropical broad-leaved evergreen species. These results have important implications for establishing mixed-species plantations for diversity conservation and improvement of ecosystem functioning in the Chinese subtropics and elsewhere. Additional costs associated with mixed-species plantations as compared to conventional plantations also demonstrate the potential of upscaling BEF experiments to large-scale afforestation projects.  相似文献   

Experimental infection of domestic cats with passaged genotype I Bartonella henselae     

Werner JA  Kasten RW  Feng S  Sykes JE  Hodzic E  Salemi MR  Barthold SW  Chomel BB 《Veterinary microbiology》2007,122(3-4):290-297

The influence of in vitro passage on Bartonella henselae pathogenesis in cats has not been thoroughly evaluated. Our objective was to examine the bacterial kinetics and humoral immune responses in cats experimentally infected with three different in vitro passages of B. henselae F1, a genotype I strain of feline origin. The F1 strain was in vitro passaged 20 and 40 times, and each was inoculated into a group of 5 cats. The kinetics of bacteremia and the feline humoral immune response to bacterial antigens were compared to a previous study involving a group of six cats inoculated with the original F1 strain. Among the three groups of cats, the kinetics of bacteremia profiles and the humoral immune responses to B. henselae lysates were similar. The influence of passage on bacterial membrane proteins was examined. In vitro passage altered the expression of 4/17 (23.5%) bacterial membrane proteins and 6/15 (40%) bacterial membrane antigens. An association between poor seroreactivity to three lysate antigens (15-, 18- and 45kDa), prolonged bacteremia and decreased serum bactericidal activity was noted. Our data show that in vitro passage of B. henselae did not alter the kinetics of bacteremia, including the occurrence of relapsing bacteremia, in experimentally infected cats. This suggests that highly passaged strains may not be suitable for future vaccination studies. Furthermore, in vitro passage results in phenotypic and antigenic changes in the bacterial membrane protein profile, which warrants caution in the interpretation of studies involving passaged B. henselae strains.  相似文献   

Nachernte-Behandlung von Äpfeln mit Calciumchlorid     

Werner Dierend  Sandra Rieken 《Erwerbs-Obstbau》2007,49(2):51-56

From 2003 to 2006 the efficacy of post-harvest dips in calcium chloride solution was investigated for apples. Aim of these investigations was to find out the influence of different factors on the Ca-uptake of apples after harvest. The Ca-uptake of the fruit was influenced by following factors:

• the calcium chloride concentration of the dip solution,
• the duration of the dip,
• the duration of fruits' wetting,
• the apple cultivar,
• the addition of a wetting agent and
• the maturity of the fruit.

To get an appreciable Ca-uptake, the addition of a wetting agent is necessary. For a dip time of two minutes calcium chloride concentrations of 7 or 7.5% are needed. Apparently injuries of the apple skin don't appear. An elongation of the dip time or the duration of wetting increased the Ca-uptake. Cultivar and maturity of the fruit affect the Ca-uptake. The increase of the Ca-content can proved only close to the apple skin (skin to 2?cm depth).  相似文献   

Frequency and mechanisms of 2n egg formation in haploid tuberosum-wild species F1 hybrids     

Joanna E. Werner  S. J. Peloquin 《American Journal of Potato Research》1987,64(12):641-654

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