Regularities and Irregularities in the Structure of the Seminiferous Epithelium in the Domestic Fowl (Gallus domesticus)     

T. Tiba    Y. Shimizu    I. Kita  T. Tsubota 《Anatomia, histologia, embryologia》1993,22(3):254-263

A cellular association demarcated by two perpendiculars which were drawn between adjacent bundles of elongate spermatids from the tubular lumen to the basement membrane, was made the unit of histometrical observation in this study (provisionally called a “column”). Cell counting revealed that the average numbers per column of various types of germ cells do not show any significant differences among 5 fowls and between paired testes. The frequency of spermiogenic steps (numbered 1–8) was investigated in each column. A definite and common pattern was found in the frequency distribution in the 5 fowls observed. A relationship between spermiation and younger spermatid steps was also investigated in each column. The spermiation was found at different steps, but most frequently at step 2 (30.6 %). Based on these observations and referring to other author's information, an average time interval between two successive spermiations was calculated roughly at 3.3 ± 1.2 days. Theoretically, this value is equal to an average length of one epithelial cycle. Such a variable cycle may have caused irregular cellular associations in this species.  相似文献   

A cross-protection experiment in pigs vaccinated with Haemophilus parasuis serovars 2 and 5 bacterins, and evaluation of a bivalent vaccine under laboratory and field conditions.   总被引：1，自引：0，他引：1  

K Takahashi  S Naga  T Yagihashi  T Ikehata  Y Nakano  K Senna  T Maruyama  J Murofushi 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(5):487-491

Cross-protection between Haemophilus parasuis serovars 2 and 5 was examined in pigs using a bacterin based vaccine, and subsequently the safety and efficacy of a bivalent vaccine were evaluated. Upon intratracheal challenge of a serovar 2 or 5 strain, pigs immunized with a monovalent vaccine were protected against challenge with a homologous serovar strain, but not with a heterologous serovar strain. Immunization with a bivalent vaccine containing both serovars 2 and 5 bacterins conferred protection in pigs against lethal challenge with each of the serovar strains. A total of 86 pigs from two SPF herds were injected with the bivalent vaccine intramuscularly twice at a four-week interval. No adverse reactions following the vaccination were observed. On day 7 after the second vaccination, vaccinated and non-vaccinated control pigs from herd A were transferred to herd B, where Glasser's disease had broken out. Pigs in the control group developed clinical signs of the disease, and 6 of 8 (75%) pigs died until slaughter, in contrast with only 4 of 46 (9%) pigs in the vaccinated group. In herd C, where there was no outbreak of Glasser's disease, complement fixation antibody titer was raised only in the vaccinated group. A challenge experiment on days 20 and 79 after the second vaccination showed that only the vaccinated pigs were protected. From these findings, the safety and efficacy of the bivalent vaccine were confirmed under laboratory and field conditions.  相似文献   

Cliniconeuropathologic findings of familial frontal lobe epilepsy in Shetland sheepdogs          下载免费PDF全文

T. Morita  A. Shimada  T. Takeuchi  Y. Hikasa  M. Sawada  S. Ohiwa  M. Takahashi  N. Kubo  T. Shibahara  H. Miyata    E. Ohama 《Canadian journal of veterinary research》2002,66(1):35-41

We examined an epileptic focus by electroencephalography (EEG) by using an international 10-20 electrode system in 11 Shetland sheep dogs affected with familial idiopathic epilepsy. We also performed an evaluation of the amino acids in the cerebrospinal fluid (CSF) and a pathologic examination of the brains of 8 dogs that died from status epilepticus. Continuous electroencephalography demonstrated that an epileptic focus was initially detected in the frontal lobe, particularly the internal area, and that paroxysmal foci developed diffusely in other lobes of affected dogs with recurrent convulsions. The EEG analyses indicated spike and sharp wave complexes, which were considered to be paroxysmal discharges. An increased value for glutamate or aspartate was found in the CSF of some epileptic dogs. Histologically, acute neuronal necrosis and astrocytosis were distributed predominantly in the cingulate cortex and internal area of frontal cortex, less frequently in other areas of the cerebrum. The results of this study suggest that, initially, the dogs have an epileptic focus in the frontal lobe, and that the focus extends gradually to other areas of the cerebrum. Based on the distribution of neuronal necrosis and astrocytosis, acute neuronal damage may be related to the superexcitation of neurons following epilepsy.  相似文献   

Inhibitory effect of heparin on Rhabdovirus and Bunyavirus isolated from cattle     

S NAKAMURA  Y ISHIHARA  K ISHIKAWA  T SHIMAZAKI  Y INOUE  H SASAKI  Y INABA 《Australian veterinary journal》1993,70(7):264-265

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几种果树多倍体与二倍体梢端细胞组织学的研究   总被引：1，自引：0，他引：1  

陈学森  郭延奎 《山东农业大学学报(自然科学版)》1991,22(2):146-154

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Fish meal vs. corn gluten meal as a protein source for dry cat food.     

M Funaba  T Tanak  M Kaneko  T Iriki  Y Hatano  M Abe 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(12):1355-1357

To compare the effects of two dietary protein sources, fish meal (FM) and corn gluten meal (CGM), fecal moisture content, nitrogen balance and urinary excretion were examined in adult cats. The dietary protein source did not cause a significant difference in daily food intake, water intake, urine volume, dry matter digestibility or urinary nitrogen excretion, but fecal moisture content was lower (P<0.02) in the CGM group. The HCl-insoluble fraction of urinary sediment tended to be higher in the CGM group (P<0.10), although urinary pH was similar in the two groups. These results suggest that CGM is comparable with FM in respect to nutritional value and the urine acidifying effect, but FM may be preferable to CGM for the prevention of constipation and struvite urolithiasis in cats.  相似文献   

Reduced Phagocytotic Activity of Macrophages in the Bovine Retained Placenta     

M Miyoshi  Y Sawamukai  & T Iwanaga 《Reproduction in domestic animals》2002,37(1):53-56

This study was carried out to investigate the distribution of immune cells in the bovine placenta during the postpartum period and to compare these cells between normal and retained placenta. Within 1 h after normal calving, biopsy samples of placentomes were collected from 10 cows. The occurrence of retention of fetal membranes was monitored for more than 8 h post-calving, and the samples obtained were divided into two groups: normally discharged and retained placenta (n = 5 each). Immunohistochemical procedures were utilized to detect macrophages and T lymphocytes. Numerous CD14-positive macrophages were found in the stroma of both normal placenta and retained placenta whereas only a few CD3-positive T lymphocytes were found in both cases. However, histochemical staining for acid phosphatase, a predominant lysozomal enzyme, revealed that almost all macrophages showed strong enzyme activity in the normally discharged placentas, whereas in retained placenta the activity of acid phosphatase was conspicuously decreased in intensity. These results indicate that there are functional differences in placental macrophages between normal and retained placenta.  相似文献   

Distinguishing male sterile and fertile cytoplasms in indica rice (Oryza sativa L.) by OPA 12     

HONG Delin  M Ichii  Y Ohara  K Chouda  S Taketa 《水稻科学》2002,10(4)

The majority of off-type plants in indica hybrid rice were CMS and its maintainer lines.  相似文献   

Caprine offspring born from fresh and frozen-thawed in vitro-produced embryos.     

Y Han  M Meintjes  K Graff  R Denniston  L Zhang  C Ziomek  R Godke 《The Veterinary record》2001,149(23):714-716

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Haemolytic anaemia and exercise intolerance due to phosphofructokinase deficiency in related springer spaniels     

E. Skibild    K. Dahlgaard    Y. Rajpurohit    B. F. Smith  U. Giger 《The Journal of small animal practice》2001,42(6):298-300

Phosphofructokinase (PFK) deficiency is an autosomal recessive inherited disorder in dogs causing haemolytic crises and exertional myopathy. The clinical signs may be confused with those of recurrent immune-mediated haemolytic anaemia. The deficiency has been commonly observed in field trial (working) English springer spaniels (ESSPs), but also in the conformation line of ESSPs in the USA over the past two decades. This report documents the first family of ESSPs found with PFK deficiency in Europe. Two related adult ESSPs in Denmark had intermittent signs of pigmenturia after exercise (hunting) and had evidence of a regenerative haemolytic anaemia. Based upon DNA sequencing data, both dogs had the previously described nonsense point mutation in the muscle-type PFK gene (delta2228G-->A). Study of 17 related family members using a simple and accurate PFK-DNA test revealed one additional PFK-deficient dog (with minor exercise intolerance), nine carriers and seven normal (or 'clear') ESSPs. Recently, the authors have also identified PFK carriers and affected ESSPs in the UK. Screening for PFK deficiency is recommended for ESSPs with suspicious clinical signs and before using any for field trials or breeding in order to prevent the further spread of this hereditary disorder.  相似文献