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11.
Koji TSUJIMURA Harutaka MURASE Hiroshi BANNAI Manabu NEMOTO Takashi YAMANAKA Takashi KONDO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1545-1548
We investigated the influences of various reaction conditions on equine
herpesvirus type 1 (EHV-1) disinfection by 5 commercial disinfectants (3 quaternary
ammonium compounds [QACs] and 2 chlorine-based disinfectants) and 1 anionic surfactant.
QACs at their highest recommended concentrations had no virucidal effect on EHV-1 with a
10-min reaction time at 0°C or a 1-min reaction time at room temperature. Chlorine-based
disinfectants achieved EHV-1 disinfection with a 10-min reaction time at −10°C or a 30-sec
reaction time at room temperature. In the presence of 5% fetal bovine serum, QACs (except
for benzalkonium chloride) showed more stable virucidal effects than did chlorine-based
disinfectants. The virucidal effect of the anionic surfactant was almost equivalent to
that of the QACs. 相似文献
12.
Hiroshi BANNAI Manabu NEMOTO Koji TSUJIMURA Takashi YAMANAKA Takashi KONDO Tomio MATSUMURA 《Journal of Equine Science》2013,24(4):71-74
Non-specific hemolysis has often been observed during complement-fixation (CF) tests for
equine herpesvirus type-1 (EHV-1), even when the sera have virus-specific CF antibodies.
This phenomenon has also been reported in CF tests for various infectious diseases of
swine. We found that the sera from 22 of 85 field horses (25.9%) showed non-specific
hemolysis during conventional CF testing for EHV-1. Because pretreatment of swine sera
with potassium periodate (KIO4) improves the CF test for swine influenza, we
applied this method to horse sera. As we expected, horse sera treated with KIO4
did not show non-specific hemolysis in the EHV-1 CF test, and precise determination of
titers was achieved. 相似文献
13.
14.
DONG Chen-fei CAI Qing-sheng WANG Cai-lin Jiro HARADA Keisuke NEMOTO SHEN Yi-xin 《水稻科学》2008,15(3):195
Genetic improvement of the digestibility of rice strew for increasing the utilization of the whole rice plant as feedstuffs is an important way to solve the feedstuffs shortage in southeastem China. To elucidate the genetic basis of the traits affecting the digestibility of rice straw, a rice population of 111 recombinant inbred lines (RILs) derived from a cross of Nekken 2xGaya was used to map the quantitative traits loci (QTLs) for in vitro dry matter digestion (IVDMD), the percentages of nonstructurel carbohydrate (NSC) and acid detergent fiber (exclusive of residual ash, ADFom) in 2005 and 2006. IVDMD was positively correlated with NSC, and negatively correlated with ADFom. A total of 16 QTLs were detected in the two years, and the amounts of variation explained by individual QTLs ranged from 6.9% to 15.5%. Some QTLs for IVDMD and ADFom on chromosome 2 were detected in the two years. On chromosome 2, the interval RM475-RM3515 contained QTLs for IVDMD in the two years and for NSC in 2006 only, while the interval RM3874-RM5305 influenced ADFom in the two years and NSC in 2005 only. At those loci, Gaya contributed favorable alleles to IVDMD and NSC, while Nekken 2 contributed positive alleles to ADFom. 相似文献
15.
Ko NAKASUMI Naoki YAMAMOTO Taro TAKAMI Harumichi ITOH Kazuhito ITAMOTO Hiro HORIKIRIZONO Toshie ISERI Munekazu NAKAICHI Yuki NEMOTO Hiroshi SUNAHARA Kenji TANI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(1):114
Transcatheter arterial embolization (TAE) and transcatheter arterial chemoembolization (TACE) are standard treatments for advanced hepatocellular carcinoma (HCC) and particularly for unresectable tumors or liver metastases in humans. However, reports on TACE used in veterinary medicine are few. This study aimed to evaluate the feasibility and safety of drug-eluting bead transarterial chemoembolization (DEB-TACE). We performed DEB-TACE in four clinically normal dogs and pharmacokinetically compared the results against hepatic arterial infusion (HAI) of cisplatin in two dogs. Drug-eluting beads (DEB) loaded with cisplatin were injected through a microcatheter for selective embolization of the left hepatic artery. After embolization, computed tomography (CT) images and histological examination findings were obtained during a 4-week observation period. Serum platinum concentrations were measured to evaluate cisplatin after each procedure. Biochemical analysis was performed during a 12-week observation period. Embolization was successful in all dogs, and there were no clinically apparent abnormalities. Embolization was confirmed up to 4 weeks after DEB-TACE in two of the four dogs and up to 1 week in the other two dogs using postoperative CT images. Cisplatin was not detected in peripheral veins in all dogs after DEB-TACE, but it was detected in trace amounts after HAI. DEB-TACE using cisplatin was safe and well tolerated by normal dogs. DEB-TACE may be useful in terms of determining systemic toxicity and drug concentration within tumors. 相似文献
16.
卵色是由卵壳色和浆膜细胞色素及卵黄色复合的颜色构成的，一般的卵呈黑（深褐）色，不同地区品种有所差异：中系品种有些为褐色大多都带点绿色呈青灰色；日系品种多为灰紫色。正常卵壳无色透照或半透明：中系品种卵壳有白色半透明，略带浅黄绿色、半透明两种，日系品种卵壳多为白色透明。灰色卵属于突变卵壳之一，在很久以前已被人们发现，灰色卵只是卵壳不透明而现乳白色，故在外观上卵色呈灰色，早年发现的灰色卵Ｇｒ的特点为：当其是纯合体时壳卵透明呈正常卵色，杂合体时卵壳不透明为灰色卵。一般情况下，蚕化蛹后应在２５℃左右温度下… 相似文献
17.
HIROKO AIDA DVM YUTAKA MIZUNO DVM PhD SEIJI HOBO DVM MS KOHEI YOSHIDA DVM MS TORU FUJINAGA DVM PhD 《Veterinary surgery : VS》1996,25(2):164-170
The effects of 1.0, 1.5, and 2.0 minimum alveolar concentration (MAC) of sevoflurane on hemodynamic, pulmonary and blood chemistry variables were measured during spontaneous and controlled ventilation in healthy horses. Sevoflurane was the only anesthetic drug administered to the horses. In a dose-dependent manner, sevoflurane significantly decreased ( P <.05) mean arterial blood pressure, cardiac output, and stroke volume. There was a progressive decrease in peripheral vascular resistance and an increase in heart rate as the concentration of sevoflurane was increased, but the differences were not significant. During spontaneous ventilation there was a dose-dependent decrease in respiratory rate that caused a decrease in the minute volume. As the dose of sevoflurane increased, the arterial carbon dioxide tension also increased ( P <.05). All blood chemistries remained within normal limits. Recovery from anesthesia was without incident. In conclusion, sevoflurane induces a dose-dependent decrease in hemodynamic variables and pulmonary function in horses that is not greatly different from that of other approved inhalant anesthetics. 相似文献
18.
Wenxi CHEN Tetsu NEMOTO Toshio KOBAYASHI Toshiyuki SAITO Etsuko KASUYA Yoshifumi HONDA 《Animal Science Journal》2002,73(6):545-551
A method of recording the fetal electrocardiogram (ECG) and heart rate (HR) is described. An abdominal lead and a thoracic lead were used to measure the fetal ECG and maternal ECG, respectively. The maternal component in the abdominal lead measurement was estimated by a digital adaptive filter using the thoracic lead measurement as a reference. By suppressing the estimated maternal components in the abdominal lead, the fetal ECG could be detected. The beat‐by‐beat fetal QRS complex peaks were determined by a digital matched filter from the fetal ECG enhanced in this way. The method was trialed in 10 pregnant Holstein cows at gestational periods ranging from 136 to 224 days. The results show that this method can extract the fetal ECG and determine the fetal HR from the raw noisy measurement data. The results suggest that the method would have applicability in monitoring fetal ECG as well as providing a non‐invasive, continuous HR profile during gestation, which will enable better understanding of the development of cattle fetuses before delivery. 相似文献
19.
Minoru OHTA Manabu NEMOTO Koji TSUJIMURA Takashi KONDO Tomio MATSUMURA 《Journal of Equine Science》2011,22(3):53-56
A PCR assay for the diagnosis of respiratory disease induced by equine herpesvirus type 1
(EHV-1) was performed at the clinical laboratory in the Racehorse Clinic of the Ritto
Training Center of the Japan Racing Association from December 2007 to March 2008. The
assay was performed without the trouble of contamination throughout the study and its
turnaround time was approximately 6 hr. The PCR detection rates of EHV-1 among
seroconverted horses were 22.2% for nasal swabs and 33.3% for blood samples. However,
EHV-1 DNA was also detected in horses without seroconversion at a low rate. These results
indicated that the PCR assay should be used as an adjunct method, but would help to make
an early diagnosis of EHV-1 infection. 相似文献
20.
ZHANGYan-ju LIUYa-guang DIAOZhuo YUTAKAHirata 《东北农业大学学报(英文版)》2004,11(2):113-117
To introduce CMV resistance to susceptible variety, interspecific grafting between d45-6 (Capsicum annuum) and LS1205 (C. baccatum) was conducted. The graft-progenies, G1 and G1S1 were obtained. Mechanical inoculation with CMV showed that disease index of G1and G1S1 were significantly decreased, similar to that of the resistant stock. EUSA serological test indicated that resistance of LS1205 and the graft-progenies to CMV was caused by inhibition of virul replication. Further, peroxidase isozymatic analysis revealed that zymotypes of G1 were a summation of those found in the donor plants. This result perfectly fit the theory of gene transformation and reconfirmed the conclusion of mechanism of graft-induced variants. 相似文献