Effects of cadmium on cork oak (Quercus suber L.) plants grown in hydroponics     

Gogorcena Y  Larbi A  Andaluz S  Carpena RO  Abadía A  Abadía J 《Tree physiology》2011,31(12):1401-1412

Cork oak (Quercus suber L.) is an autochthonous tree species that is being used for reforestation in heavy-metal-contaminated areas in Spain. A hydroponics experiment was carried out to characterize the effects of Cd on several morphological and physiological parameters in this species, including shoot length, nutrient concentrations and allocation in different organs, leaf pigment concentrations, photosynthetic efficiency, root ferric chelate reductase (FCR) activity and organic acid concentrations in xylem sap. Four different Cd treatments were applied, adding Cd chelated with EDTA or as chloride salt at two different concentrations (10 and 50 μM Cd). After 1 month of Cd treatment, plant growth was significantly inhibited in all treatments. Results indicate that Cd accumulates in all organs 7- to 500-fold when compared with control plants. The highest Cd concentration was found in the 50 μM CdCl(2) treatment, which led to concentrations of ~30, 123 and 1153 μg Cd g(-1) dry weight in leaves, stems and roots, respectively. In the strongest Cd treatments the concentrations of P and Ca decreased in some plant parts, whereas the Mn leaf concentrations decreased with three of the four Cd treatments applied. The concentrations of chlorophyll and carotenoids on an area basis decreased, whereas the (zeaxanthin plus antheraxanthin)/(total violaxanthin cycle carotenoids) ratio and the non-photochemical quenching increased significantly in all Cd treatments. Cadmium treatments caused significant increases in the activity of the enzyme FCR in roots and in the concentrations of organic acids in xylem sap. Some of the physiological changes found support the fact that Cd induces a deficiency of Fe in cork oak, although the plant Fe concentrations were not reduced significantly. At higher concentrations the effects of Cd were more pronounced, and were more marked when Cd was in the free ion form than when present in the form of Cd-EDTA.  相似文献   

Serum acute phase proteins as biomarkers of pleuritis and cranio-ventral pulmonary consolidation in slaughter-aged pigs     

Saco Y  Fraile L  Giménez M  Alegre A  López-Jimenez R  Cortey M  Segalés J  Bassols A 《Research in veterinary science》2011,91(1):52-67

The purpose of this study was to investigate the relationship between the existence of lung lesions in pigs at slaughter and the concentration of the serum acute phase proteins (APP), haptoglobin (Hp), pig-major acute protein (Pig-MAP) and C-reactive protein (CRP). A total of 24 pig farms were selected out of a larger farm database previously screened to study risk factors associated with pleuritis and cranio-ventral pulmonary consolidation (CVPC) lesions at slaughter-aged pigs in Spain. The farms were classified as “pleuritis negative (P−) or positive (P+)” and as “CVPC negative (M−) or positive (M+)” and divided into four groups according to a 2X2 factorial design (P−M−, P−M+, P+M−, P+M+). Also at slaughter, blood from 20 randomly selected pigs from each farm was collected. Obtained serum samples were used to measure acute phase proteins. All APP concentrations were significantly higher for M+ farms than for M− ones. However, only Hp and Pig-MAP showed significantly higher concentrations for P+ farms than for P− ones. Pig-MAP was the most sensitive biomarker since it was able to clearly discriminate between P−/P+ and M−/M+ groups (p < 0.001 in both cases). Hp was an excellent marker for pleuritis and good for CVPC lesions. CRP was able to discriminate for CVPC lesions but not for pleuritis. The present results indicate that Pig-MAP and, possibly Hp, may be used as potential markers to characterise and discriminate respiratory lesions in swine herds at slaughter.  相似文献   

Influence of Bifidobacterium longum CECT 7347 and gliadin peptides on intestinal epithelial cell proteome     

Olivares M  Laparra M  Sanz Y 《Journal of agricultural and food chemistry》2011,59(14):7666-7671

Celiac disease is an enteropathy caused by an abnormal immune response to cereal gluten proteins (gliadin). To unravel the possible role of the interactions between gliadin peptides and specific intestinal bacteria, the response of intestinal epithelial (Caco-2) cells to gliadin subjected to gastrointestinal digestion in the presence or absence of Bifidobacterium longum CECT 7347 has been studied. Changes in the proteome of Caco-2 cells were determined by 2DE and MALDI-TOF. Gliadins digested without B. longum altered the expression of a higher number of proteins than in the presence of the bacterium (21 versus 9), and these proteins were involved in disorganization of cell cytoskeleton, inflammation, and apoptosis. Gliadins digested in the presence of the bacterium influenced the production of proteins involved in calcium homeostasis and cell survival and function. Therefore, B. longum CECT 7347 might ameliorate gliadin toxicity and modify the responses of intestinal epithelial cells to the gliadin challenge.  相似文献   

Morphological and molecular variability in some Iranian almond genotypes and related Prunus species and their potentials for rootstock breeding     

Kianoush Nikoumanesh  Ali Ebadi  Mehrshad Zeinalabedini  Yolanda Gogorcena 《Scientia Horticulturae》2011

In this study, in order to know the variability for a rootstock breeding program genetic diversity and relationships among 55 Iranian almond genotypes and seven related Prunus species were investigated. Morphological and molecular analyses were used. Principal component analysis showed that three components explained 67.6% of the total morphological variation for the first year and 68.06% for the second year of the study. Leaf traits were predominant in the first component and contributed most of the total variation. Leaf length and width, as well as, leaf area were highly correlated with each other and correlated to vigor. Also a negative correlation was found between leaf length/width ratio and vigor. Ward's method was used to construct cluster from morphological data which allocated individuals into their respective species. Out of 100 pre-screened RAPD primers, 16 with reproducible bands and maximum polymorphism were selected. Two-hundred and sixty bands were scored of which 250 of them were polymorphic. Average value of polymorphism per primer was 95.81% and maximum value for polymorphism (100%) was obtained from TIBMBA-14, TIBMBA-17, TIBMBB-05, TIBMBB-08, TIBMBD-09, and TIBMBD-10. On the other hand, the minimum value was obtained from TIBMBB-16 (86%). Primer TIBMBB-5 gave the maximum number of bands (25 fragments) and the minimum obtained from TIBMBE-18 (11 fragments). Genetic similarity based on Jaccard's coefficient ranged from 0.28 to 0.79 with an average of 0.53. Molecular analysis revealed a high degree of separation among samples regarding their geographical origin. Correlation between two approaches was low (R = −0.38). High molecular and morphological variability indicated that this collection includes rich and valuable plant materials for almond rootstock breeding.  相似文献   

Heuristic algorithms vs. linear programs for designing efficient conservation reserve networks: Evaluation of solution optimality and processing time     

Robert P.D. Vanderkam  Yolanda F. Wiersma 《Biological conservation》2007,137(3):349-358

Systematic approaches to efficient reserve network design often make use of one of two types of site selection algorithm; linear programs or heuristic algorithms. Unlike with linear programs, heuristic algorithms have been demonstrated to yield suboptimal networks in that more sites are selected in order to meet conservation goals than may be necessary or fewer features are captured than is possible. Although the degree of suboptimality is not known when using heuristics, some researchers have suggested that it is not significant in most cases and that heuristics are preferred since they are more flexible and can yield a solution more quickly. Using eight binary datasets, we demonstrate that suboptimality of numbers of sites selected and biodiversity features protected can occur to various degrees depending on the dataset, the model design, and the type of heuristic applied, and that processing time is not dramatically different between optimal and heuristic algorithms. In choosing an algorithm, the degree of suboptimality may not always be as important to planners as the perception that optimal solvers have feasibility issues, and therefore heuristic algorithms might continue to be a popular tool for conservation planning. We conclude that for many datasets, feasibility of optimal algorithms should not be a concern and that the value of heuristic results can be greatly improved by using optimal algorithms to determine the degree of suboptimality of the results.  相似文献   

Influence of Dehydration Process in Castellano Chickpea: Changes in Bioactive Carbohydrates and Functional Properties     

Aguilera Y  Benítez V  Mollá E  Esteban RM  Martín-Cabrejas MA 《Plant foods for human nutrition (Dordrecht, Netherlands)》2011,66(4):391-400

Changes in bioactive carbohydrates, functional, and microstructural characteristics that occurred in chickpea under soaking, cooking, and industrial dehydration processing were evaluated. Raw chickpea exhibited important levels of raffinose family of oligosaccharides (RFOs), resistant starch (RS) and total dietary fibre (TDF), being insoluble dietary fibre (IDF) the main fraction (94%). The dehydration process increased RFOs (43%), RS (47%) and soluble dietary fiber (SDF) (59%) levels significantly. In addition, a noticeable increase in both fibre fractions was observed, being higher in soluble fibre in (SDF) (59%). The minimum nitrogen solubility of raw flours was at pH 4, and a high degree of protein insolubilization (80%) was observed in dehydrated flours. The raw and processed flours exhibited low oil-holding capacities (1.10 mg/ml), and did not show any change by thermal processing, whereas water-holding capacities rose to 5.50 mg/ml of sample. Cooking and industrial dehydration process reduced emulsifying activity and foaming capacity of chickpea flour. The microstructural observations were consistent with the chemical results. Thus, the significant occurrence of these bioactive carbohydrate compounds along with the interesting functional properties of the dehydrated flours could be considered useful as functional ingredients for food formulation.  相似文献   

Glycemic Response to Extruded Oat Bran Cereals Processed to Vary in Molecular Weight     

Yolanda Brummer  Ruedi Duss  Thomas M. S. Wolever  Susan M. Tosh 《Cereal Chemistry》2012,89(5):255-261

Oat β‐glucan enriched extruded cereals with molecular weights (MWs) ranging from 2,180,000 to 326,000 were produced. Test meals composed of 31 g of available carbohydrate, 8.3–8.7 grams of β‐glucan, and milk were administered to subjects and their postprandial blood glucose levels monitored for 2 hr. White bread plus milk and a control wheat bran cereal plus milk were administered, and white bread alone served as a reference food for estimation of glycemic index (GI) of the cereals, after adjusting for the effect of adding milk to white bread. Both oat bran and wheat bran cereals significantly reduced peak blood glucose rise (PBGR) and area under the curve (AUC) versus white bread alone or white bread plus milk. There was a significant inverse relationship between AUC and log₁₀[weight average MW] (r² = 0.96, P = 0.0192). There was a significant inverse relationship between PBGR and log₁₀[viscosity] of in vitro extracts from all cereals, including the wheat control (r² = 0.96, P = 0.0031). However, no significant differences in glycemic responses among the oat bran cereals were found with pairwise comparisons. All cereals were low GI (<55) and were significantly lower than white bread alone or white bread plus milk. Among the oat bran cereals, palatability was positively correlated with MW (r² = 0.98, P = 0.0110).  相似文献   

Nutrient removal efficiency of Hydropuntia cornea in an integrated closed recirculation system with pink shrimp Farfantepenaeus brasiliensis     

Daniel Robledo  Leonardo Navarro‐Angulo  David Valdes Lozano  Yolanda Freile‐Pelegrín 《Aquaculture Research》2014,45(10):1648-1658

In this study, we have tested the effect of seaweed stocking density in an experimental seaweed biofilter using the economically important red seaweed Hydropuntia cornea integrated with the cultivation of the pink shrimp Farfantepenaeus brasiliensis. Nutrient removal efficiency was evaluated in relation to seaweed stocking density (2.5, 4, 6 and 8 g fw L^?1). Total ammonia nitrogen (TAN) was the main nitrogen source excreted by F. brasiliensis, with concentrations ranging from 41.6 to 65 μM of NH₄⁺‐N. H. cornea specific growth rates ranged from 0.8 ± 0.2 to 1.4 ± 0.5% day^?1 with lowest growth rates at higher seaweed stocking density (8 g fw L^?1). Nutrient removal was positively correlated with the cultivation densities in the system. TAN removal efficiency increased from 61 to 88.5% with increasing seaweed stocking density. Changes in the chemical composition of the seaweed were analysed and correlated with nutrient enrichment from shrimp effluent. The red seaweed H. cornea can be cultured and used to remove nutrients from shrimp effluents in an integrated multi‐trophic aquaculture system applied to a closed recirculation system. Recirculation through seaweed biofilters in land‐based intensive aquaculture farms can also be a tool to increase recirculation practices and establish full recirculation aquaculture systems (RAS) with all their known associated benefits.  相似文献   

Selenium species bioaccessibility in enriched radish (Raphanus sativus): a potential dietary source of selenium     

Pedrero Z  Madrid Y  Cámara C 《Journal of agricultural and food chemistry》2006,54(6):2412-2417

An in vitro gastrointestinal method was employed to predict the potential bioavailability of selenium and its species from radish, belonging to the Brassicaceae family, grown in hydroponics media in the presence of inorganic selenium, such as Na2SeO3 and Na2SeO4. A low transformation of Se into organic forms was observed in radish plants grown in Se(VI)-enriched culture media. On the contrary, in those plants exposed to selenite, >95% of the total selenium was found as selenocystine (SeCys2), selenomethionine (SeMet), and Se-methylselenocysteine (SeMetSeCys). The concentrations of these species in fresh samples remained almost unaltered after a simulated gastrointestinal digestion. Therefore, a high selenium content of Se-methylselenocysteine (65%), previously reported as a cancer chemopreventive species, remained in the potentially bioabsorbable fraction. As these plants usually undergo a short development cycle, these results suggest that radish enriched in selenite could be a good choice as an organoselenium supplement for the human diet and animal feed.  相似文献   

Effect of Drying on the Phenolic Content and Antioxidant Activity of Red Grape Pomace     

Yolanda Carmona-Jiménez  M. Valme García-Moreno  Carmelo García-Barroso 《Plant foods for human nutrition (Dordrecht, Netherlands)》2018,73(1):74-81

Winemaking by-products are considered to be a rich source of bioactive compounds. Grape pomace is susceptible to microbial degradation due to the degree of residual moisture, so the drying of this pomace for conservation is considered to be an essential first step. Previous studies concerning the way in which drying affects winery by-products have produced contradictory results. In this study, a new methodology for drying grape pomace in a climatic chamber has been evaluated. Five red grape pomace varieties were dried in a climatic chamber at 40 °C and 10% relative humidity and the phenolic content and antioxidant activity of the dried and wet pomace samples were compared. The results indicate that this drying process is both feasible and beneficial because significant increases in the extractability of phenolic compounds and antioxidant activity were achieved.  相似文献