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11.
Isolation of Neospora caninum from the brain of a naturally infected adult dairy cow 总被引:1,自引:0,他引:1
Sawada M Kondo H Tomioka Y Park C Morita T Shimada A Umemura T 《Veterinary parasitology》2000,90(3):247-252
Neospora caninum was isolated from the brain of a 2-year-old dairy cow that had aborted confirmed N. caninum-infected fetuses on two occasions. The cow had an indirect fluorescent antibody titer of 1:1600 to N. caninum. The cow was killed 24 days after its second abortion and the brain was bioassayed for N. caninum in nude mice. Multifocal areas of perivascular cuffing and glial nodules were observed in the cerebrum and mesencephalon of the cow, but N. caninum was not identified in histological sections of the brain. All three nude mice inoculated with brain homogenate of the cow, developed emaciation and paralysis. Microscopical examination of the nude mice revealed systemic N. caninum infection with demonstrable tachyzoites in various organs. The parasites isolated from fresh mouse brain were transferred successfully into Vero cell cultures. PCR procedure on the purified tachyzoites obtained from the Vero cell cultures amplified the specific DNA sequence for N. caninum. 相似文献
12.
A 10-year survey of antimicrobial susceptibility of streptococcus suis isolates from swine in Japan 总被引:7,自引:0,他引:7
Kataoka Y Yoshida T Sawada T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(10):1053-1057
A number of 689 Streptococcus suis isolates collected nationwide from diseased and healthy pigs from 1987 to 1996 were surveyed for antibiotic susceptibilities to 11 drugs. No isolates resistant to amoxicillin, chloramphenicol, and sulfamethoxazole/trimethoprim were found. Isolates were highly susceptible to penicillins (penicillin G, ampicillin, and amoxicillin) except cloxacillin. They were not susceptible to tetracycline, streptomycin, and kanamaycin (MIC90 50 microg/ml, > or = 100 microg/ml, and > or = 100 microg/ml, respectively). Multiple-resistant isolates (> or = 3 antimicrobial agents) were found in 20.3% of all isolates tested. 相似文献
13.
Journal of Plant Diseases and Protection - The antagonistic rhizobacterium, Pseudomonas fluorescens strain LRB3W1, controls cabbage Fusarium yellows caused by Fusarium oxysporum f. sp. conglutinans... 相似文献
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Antibodies against Pasteurella multocida capsular types B and E, which cause hemorrhagic septicemia, were demonstrated in a high percentage of sera from domestic feeder calves. Since the calves had not been vaccinated with any Pasteurella organisms, the antibodies were considered to be naturally acquired. The antibodies were demonstrated by passively immunizing mice and by indirect hemagglutination and agglutination tests. Sera from 81% (44 of 54) of the calves protected mice that were challenge exposed with a capsular type B organism, and sera from 91% (49 of 54) of the calves protected mice that were challenge exposed with a capsular type E organism. Indirect hemagglutination and agglutination tests demonstrated antibody in nearly all sera. Since capsular type E organisms have been isolated only in Africa and there is only 1 report of capsular type B isolation from cattle in the United States, these organisms were not considered likely sources of the antigenic stimulation that provoked production of these antibodies. 相似文献
16.
Yuko?FujiwaraEmail author Yoshihisa?Fujii Yutaka?Sawada Shogo?Okumura 《Journal of Wood Science》2004,50(1):35-40
Japanese oak and Japanese beech were sanded by hand with abrasive papers of varying grit number. Two three-dimensional parameters selected to characterize their surface roughness – one parameter for the distribution of roughness-profile peaks and the other for the relative area of the roughness-profile peaks above the threshold height – were compared against tactile roughness. The parameters were obtained from roughness profiles as determined by a robust Gaussian regression filter (RGRF) using seven cutoffs. The RGRF filtering process was adjusted specifically for the evaluation of wood surface roughness. Except for a cutoff wavelength of 0.25mm, the RGRF lent itself well to the determination of roughness profiles. No distortion of roughness profiles occurred around deep valleys, and there was a good correlation between the parameters and tactile roughness.Part of this study was presented at the 2002 Kansai Branch Office lecture meeting of the Japan Society for Precision Engineering, Kyotanabe, August 2002 相似文献
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Masatomi Hosoi Chuya Shinzato Masaya Takagi Shoko Hosoi-Tanabe Hideki Sawada Eri Terasawa Haruhiko Toyohara 《Fisheries Science》2007,73(2):385-394
ABSTRACT: Taurine is the primary osmolyte in marine molluscs, whose cellular osmo-conforming process is vital for environmental adaptation because of a lack of osmotic homeostasis. Here, cDNA cloning and expression, and functional analyses of taurine transporter (TAUT) from the giant Pacific oyster are reported on. The deduced amino-acid sequence of oyster TAUT (oyTAUT) showed 47–51% identity to those of vertebrate TAUT, whereas identity among the vertebrates is 78–95%. Functional analysis of oyTAUT expressed in Xenopus oocytes revealed that oyTAUT has a lower affinity and specificity for taurine and a requirement for higher NaCl concentration, compared with vertebrate TAUT. Taken together with similar functional properties of TAUT from mussel, indicated by our previous study, it is possible that these functional features reflect the internal environment of the molluscs (i.e. higher taurine and NaCl concentrations). Oyster taurine transporter mRNA expression was induced by not only hyper-osmotic stress, similar to other TAUT, but also hypo-osmotic stress. It is speculated that the expression in response to hypo-osmotic stress was induced by a substantial decrease in tissue taurine content following the decrease in the internal osmolality. 相似文献
19.
Shinsuke Torisawa Tsutomu Takagi Yasunori Ishibashi Yoshifumi Sawada Takeshi Yamane 《Fisheries Science》2007,73(5):1202-1204
20.
Makiguchi Y Konno Y Konishi K Miyoshi K Sakashita T Nii H Nakao K Ueda H 《Fish physiology and biochemistry》2011,37(2):273-284
The movements of 28 adult chum salmon, Oncorhynchus keta (Walbaum) tagged with electromyogram (EMG) transmitters were tracked along the Toyohira river, Hokkaido, Japan, in October
of 2007 and 2008 to investigate and evaluate the upstream migratory behavior through the protection bed and fishway of ground
sills. The approach time of fish that ascended successfully through the protection bed and fishway was shorter than that of
unsuccessful fish. The unsuccessful fish were observed to swim in currents with high water velocity and shallow water depth
at swimming speeds that exceeded their critical swimming speed (U
crit) during the approach to these structures. In consequence, unsuccessful fish frequently alternated between burst and maximum
sustained speeds without ever ascending the fishway, and eventually became exhausted. It is important that fishway are constructed
to enable chum salmon to find a passage way easily, so that they can migrate upstream rapidly without wasting excessive energy. 相似文献