Effects of time and duration of rearing with bottom sand on the occurrence and expansion of staining-type hypermelanosis in the Japanese flounder Paralichthys olivaceus     

Toshinori Isojima  Naoshi Makino  Yoshifumi Miyama  Masatomo Tagawa 《Fisheries Science》2014,80(4):785-794

We previously reported that the progression of staining-type hypermelanosis spontaneously ceased at a specific time and area in Japanese flounder Paralichthys olivaceus. To examine whether time is a limiting factor in the spontaneous cessation of staining, we experimentally controlled the initiation and duration of staining by manipulating the bottom substrate condition in the fish tanks. At 151 days post hatching (DPH; 11 weeks), spontaneous cessation of staining was observed in fish reared in tanks without a sandy substrate. However, staining resumed (or was initiated) in tanks where sand was removed from 11 weeks, indicating a strong but temporary effect of bottom sand and the absence of time limitation in the staining progression by 151 DPH. Extended duration of the inhibitory period of hypermelanosis expansion (9 weeks or more) aided in only a 20 % reduction of the final staining area because of the increased rate of staining expansion. The bottom sandy substrate decreased the visibility of the staining area in individuals, but this was observed only before the completion of the staining expansion. These findings are discussed in relation to possible presence of area limitation of future staining, as well as the fundamental nature of staining.  相似文献   

Improved porcine model for Shiga toxin‐producing Escherichia coli infection by deprivation of colostrum feeding in newborn piglets          下载免费PDF全文

Toshio Sato  Takashi Hamabata  Eiji Takita  Takeshi Matsui  Kazutoshi Sawada  Taishi Imaoka  Nobuo Nakanishi  Keizo Nakayama  Takamitsu Tsukahara 《Animal Science Journal》2017,88(5):826-831

Porcine edema disease (ED) is a toxemia caused by enteric infection with Shiga toxin 2e (Stx2e)‐producing Escherichia coli (STEC). ED occurs most frequently during the weaning period and is manifested as emaciation associated with high mortality. In our experimental infection with a specific STEC strain, we failed to cause the suppression of weight gain in piglets, which is a typical symptom of ED, in two consecutive experiments. Therefore, we examined the effects of deprivation of colostrum on the sensitivity of newborn piglets to STEC infection. Neonatal pigs were categorized into two groups: one fed artificial milk instead of colostrum in the first 24 h after birth and then returned to the care of their mother, the other breastfed by a surrogate mother until weaning. The oral challenge with 10¹¹ colony‐forming units of virulent STEC strain on days 25, 26 and 27 caused suppression of weight gain and other ED symptoms in both groups, suggesting that colostrum deprivation from piglets was effective in enhancing susceptibility to STEC. Two successive STEC infection experiments using colostrum‐deprived piglets reproduced this result, leading us to conclude that this improved ED piglet model is more sensitive to STEC infection than the previously established models.  相似文献   

Physiological effects of wood on humans: a review     

Harumi Ikei  Chorong Song  Yoshifumi Miyazaki 《Journal of Wood Science》2017,63(1):1-23

It is empirically known that wood can cause a comfort enhancement effect in humans. On the other hand, not enough scientific knowledge based on evidence-based research is available on this subject. However, data using physiological indices have increasingly accumulated in recent years. This review provides an overview of the current situation for peer-reviewed reports related to the physiological effects of wood. We reviewed reports that elucidated the effects of wood-derived stimulations on the olfactory, visual, auditory, and tactile sensations using physiological indices such as brain activity (e.g., near-infrared spectroscopy) and autonomic nervous activity (e.g., heart rate variability and blood pressure). It became clear that many studies were limited by (1) a small number of participants, mostly aged in their 20s; (2) use of only a single stimulus (e.g., only olfactory or only visual), or (3) an incomplete experimental design. In addition, this review examined the field of forest therapy, for which there is abundant research. Further study is needed to elucidate the physiological effects of wood on humans.  相似文献   

Inhibition of capsular protein synthesis of Pasteurella multocida strain P-1059     

Sthitmatee N  Kataoka Y  Sawada T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(11):1445-1451

A mutant strain, PBA322, was constructed by electroporation of a phagemid containing the coding region of antisense RNA of the ompH gene, encoding 39 kDa capsular protein or OmpH, into the parental strain P-1059 (serovar A:3) of Pasteurella multocida, and the pathogenicity was determined in mice and chickens. Grayish colonies of the mutant, indicating loss of capsule synthesis, were observed under a stereomicroscope using obliquely transmitted light, while iridescent colonies were observed for the parental strain. Moreover, strain PBA322 showed a low amount of OmpH compared with the parental strain on SDS-PAGE. Additionally, the capsule of strain PBA322 was thinner than that of the parental strain according to electron microscopy, correlating to the attenuation against chickens. In conclusion, strain PBA322, the mutant of P. multocida strain P-1059, was completely attenuated for chickens.  相似文献   

Phylogenetic groups and cephalosporin resistance genes of Escherichia coli from diseased food-producing animals in Japan     

Tetsuo Asai  Kaori Masani  Chizuru Sato  Mototaka Hiki  Masaru Usui  Kotaro Baba  Manao Ozawa  Kazuki Harada  Hiroshi Aoki  Takuo Sawada 《Acta veterinaria Scandinavica》2011,53(1):52

A total of 318 Escherichia coli isolates obtained from different food-producing animals affected with colibacillosis between 2001 and 2006 were subjected to phylogenetic analysis: 72 bovine isolates, 89 poultry isolates and 157 porcine isolates. Overall, the phylogenetic group A was predominant in isolates from cattle (36/72, 50%) and pigs (101/157, 64.3%) whereas groups A (44/89, 49.4%) and D (40/89, 44.9%) were predominant in isolates from poultry. In addition, group B2 was not found among diseased food-producing animals except for a poultry isolate. Thus, the phylogenetic group distribution of E. coli from diseased animals was different by animal species. Among the 318 isolates, cefazolin resistance (minimum inhibitory concentrations: ≥32 μg/ml) was found in six bovine isolates, 29 poultry isolates and three porcine isolates. Of them, 11 isolates (nine from poultry and two from cattle) produced extended spectrum β-lactamase (ESBL). The two bovine isolates produced bla_CTX-M-2, while the nine poultry isolates produced bla_CTX-M-25 (4), bla_SHV-2 (3), bla_CTX-M-15 (1) and bla_CTX-M-2 (1). Thus, our results showed that several types of ESBL were identified and three types of β-lactamase (SHV-2, CTX-M-25 and CTX-M-15) were observed for the first time in E. coli from diseased animals in Japan.  相似文献   

Effects of contact with wood on blood pressure and subjective evaluation     

Satoshi Sakuragawa  Tomoyuki Kaneko  Yoshifumi Miyazaki 《Journal of Wood Science》2008,54(2):107-113

This study examined the effects of contact with wood on the living human body using a physiological index and subjective evaluation. Consecutive blood pressure measurements were used as the physiological index, and sensory evaluation using the semantic differential (SD) method was used for subjective evaluation. Consideration was also given to cases in which materials were cooled and heated as well as kept at room temperature, to eliminate the effects of heat flux due to differences in thermal conductivity between wood and other materials. It was found that contact with wood produced coarse/natural sensations, with no associated increase in systolic blood pressure. Contact with cold wood created subjectively dangerous/uncomfortable but still coarse/natural sensations, also with no associated increase in blood pressure; therefore, there was no correspondence between subjective evaluation and physiological responses. Contact with aluminum kept at room temperature and cold acrylic plastic created flat/artificial and dangerous/uncomfortable sensations, with an associated significant increase in blood pressure; thus, there was a close correlation between subjective evaluation and physiological responses. It was therefore concluded that contact with wood, unlike artificial materials such as aluminum, induces no physiological stress even when kept at room temperature or cooled. Part of this report was presented at the 48th Annual Meeting of the Japan Wood Research Society in Shizuoka, April 1998  相似文献   

Pathogenicity of Erysipelothrix rhusiopathiae strains of serovars 1a, 3, 5, 6, 8, 11, 21, and type N isolated from slaughter pigs affected with chronic erysipelas   总被引：2，自引：0，他引：2  

T Takahashi  T Sawada  K Seto  M Muramatsu  T Maruyama  M Kanzaki 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1985,47(1):1-8

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Plasma estrone, estradiol-17 beta, and progesterone levels during late pregnancy and parturition in dairy cattle     

T Sawada  E Kimura  Y Fujimoto  H Matsunaga  J Mori 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1988,50(3):654-658

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Correction to: Dispersion and degradation of environmental DNA from caged fish in a marine environment     

Murakami  Hiroaki  Yoon  Seokjin  Kasai  Akihide  Minamoto  Toshifumi  Yamamoto  Satoshi  Sakata  Masayuki K.  Horiuchi  Tomoya  Sawada  Hideki  Kondoh  Michio  Yamashita  Yoh  Masuda  Reiji 《Fisheries Science》2019,85(6):1109-1109

Fisheries Science - In the original publication the text in right column of page 330, the sequences of primers were incorrectly published as.  相似文献   

Physiological effects in humans induced by the visual stimulation of room interiors with different wood quantities     

Yuko Tsunetsugu  Yoshifumi Miyazaki  Hiroshi Sato 《Journal of Wood Science》2007,53(1):11-16

To clarify the visual effects of room interior with wooden materials on humans, pulse rate, blood pressure, and brain activity were measured while the subjects were exposed to visual stimuli using actual-size model rooms. The wood ratios (the ratio of the area covered with wooden material to the whole area of the ceiling, walls, and floor) of the rooms were 0%, 45%, and 90%. Subjective evaluation was also conducted. In the 0% room, diastolic blood pressure decreased significantly, but the observed change in the autonomic nervous activity was relatively small. In the 45% room, a significant decrease in the diastolic blood pressure and a significant increase in pulse rate were observed. This room tended to have the highest scores in subjective “comfortable” feeling. The 90% room caused significant and large decreases in systolic blood pressure and diastolic blood pressure at the beginning of the test, but the large coverage of wood appeared to cause a rapid decrease in brain activity and an increase in pulse rate. The present study demonstrated that a difference in wood ratio in the interior caused different physiological responses, especially in the autonomic nervous activity, by using actual-size rooms for the first time.  相似文献