Redirection of silencing targets by adenosine-to-inosine editing of miRNAs   总被引：1，自引：0，他引：1  

Kawahara Y  Zinshteyn B  Sethupathy P  Iizasa H  Hatzigeorgiou AG  Nishikura K 《Science (New York, N.Y.)》2007,315(5815):1137-1140

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Geology. The story of O2     

Falkowski PG  Isozaki Y 《Science (New York, N.Y.)》2008,322(5901):540-542

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Insecticidal effect of phthalides and furanocoumarins from Angelica acutiloba against Drosophila melanogaster     

Miyazawa M  Tsukamoto T  Anzai J  Ishikawa Y 《Journal of agricultural and food chemistry》2004,52(14):4401-4405

Insecticidal activity of Angelica acutiloba extract and its constituents was investigated and compared with that of rotenone. Bioassay-guided isolation of the chloroform extract of A. acutiloba against larvae of Drosophila melanogaster afforded two phthalides, (Z)-butylidenephthalide (1) and (Z)-ligustilide (2), and two furanocoumarins, xanthotoxin (3) and isopimpinellin (4). The structures of these compounds were established by spectroscopic analysis. The isolated compounds 1, 2, 3, and 4 exhibited LC(50) values of 0.94, 2.54, 3.35, and 0.82 micromol/mL of diet concentration against larvae of D. melanogaster, respectively. Against both sexes (males/females, 1:1) of adults (5-7 days old), compound 1 showed the most potent activity with a LD(50) value of 0.84 microg/adult. Compound 1 is a more active insecticide than rotenone (LD(50) = 3.68 microg/adult) and has potential as a novel insect control agent. However, compound 2 was inactive against adults. The structure-activity relationship of phthalides isolated indicated that the aromaticity appeared to play an important role in the activity of both larvae and adults. To determine the insecticide mode of action for acute adulticidal activity, acetylcholinesterase (AChE) inhibitory activity was also investigated in vitro, and the result indicated that the acute adulticidal activity of compounds 3 and 4 was due to the inhibition of AChE.  相似文献   

Application of activable tracers to investigate radial movement of minerals in the stem of Japanese cedar (<Emphasis Type="Italic">Cryptomeria japonica</Emphasis>)     

Naoki Okada  Yasuhiko Hirakawa  Yukio Katayama 《Journal of Wood Science》2011,57(5):421-428

Two activable tracers, Rb and Eu, were injected into the sapwood of Japanese cedars (Cryptomeria japonica D. Don) to investigate the radial movement of minerals in their stems in the resting period. Eight trees of four cultivars, two of which genetically form wet heartwood, were treated near the end of the growing period. At 40 days after the treatment, Rb was detected in the outer heartwood, whereas Eu was not. Radial movement of Rb was more rapid in trees with wet heartwood than in those with normal heartwood. At 204 days after the treatment, more Rb was detected in the heartwood than was found on the first sampling, whereas no Eu was detected in the heartwood. The difference in radial movement between Rb and Eu was considered mainly to be the result of selective transport of beneficial minerals by Japanese cedar. The difference in the rate of radial movement of Rb between wet and normal heartwood became more conspicuous at 204 days after treatment. We concluded that the movement of Rb from the sapwood to the outer heartwood was by active transport through the rays, whereas that in the heartwood was by diffusion due to the gradient of Rb concentration.  相似文献   

Production and characterization of the novel CMS line of radish (Raphanus sativus) carrying Brassica maurorum cytoplasm     

SangWoo Bang  Kota Tsutsui  SuHyeun Shim  Yukio Kaneko 《Plant Breeding》2011,130(3):410-412

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Production and characterization of an alloplasmic and monosomic addition line of Brassica rapa carrying the cytoplasm and one chromosome of Moricandia arvensis     

Kota Tsutsui  Bum Hee Jeong  Yukiko Ito  Sang Woo Bang  Yukio Kaneko 《Breeding Science》2011,61(4):373-379

Intergeneric hybridization was performed between Moricandia arvensis and four inbred lines of Brassica rapa following embryo rescue. Three F₁ hybrid plants were developed from three cross combinations of M. arvensis × B. rapa, and amphidiploids were synthesized by colchicine treatment. Six BC₁ plants were generated from a single cross combination of amphidipolid × B. rapa ‘Ko1-303’ through embryo rescue. One BC₂ and three BC₃ plants were obtained from successive backcrossing with B. rapa ‘Ko1-303’ employing embryo rescue. Alloplasmic and monosomic addition lines of B. rapa (Allo-MALs, 2n = 21) were obtained from backcrossed progeny of three BC₃ plants (2n = 21, 22 and 23) without embryo rescue. An alloplasmic line of B. rapa (2n = 20) degenerated before floliation on 1/2 MS medium due to severe chlorosis. Allo-MALs of B. rapa (2n = 21) showed stable male sterility without any abnormal traits in vegetative growth and female fertility. Molecular analyses revealed that the same chromosome and cytoplasm of M. arvensis had been added to each Allo-MAL of B. rapa. This Allo-MAL of B. rapa may be useful material for producing cytoplasmic male sterile lines of B. rapa.  相似文献   

Occurrence of metaxenia and false hybrids in Brassica juncea L. cv. Kikarashina × B. napus     

Mai Tsuda  Ken-ichi Konagaya  Ayako Okuzaki  Yukio Kaneko  Yutaka Tabei 《Breeding Science》2011,61(4):358-365

Imported genetically modified (GM) canola (Brassica napus) is approved by Japanese law. Some GM canola varieties have been found around importation sites, and there is public concern that these may have any harmful effects on related species such as reduction of wild relatives. Because B. juncea is distributed throughout Japan and is known to be high crossability with B. napus, it is assumed to be a recipient of B. napus. However, there are few reports for introgression of cross-combination in B. juncea × B. napus. To assess crossability, we artificially pollinated B. juncea with B. napus. After harvesting a large number of progeny seeds, we observed false hybrids and metaxenia of seed coats. Seed coat color was classified into four categories and false hybrids were confirmed by morphological characteristics and random amplified polymorphic DNA (RAPD) markers. Furthermore, the occurrence of false hybrids was affected by varietal differences in B. napus, whereas that of metaxenia was related to hybridity. Therefore, we suggest that metaxenia can be used as a marker for hybrid identification in B. juncea L. cv. Kikarashina × B. napus. Our results suggest that hybrid productivity in B. juncea × B. napus should not be evaluated by only seed productivity, crossability ought to be assessed the detection of true hybrids.  相似文献   

Gene expression in individual bovine somatic cell cloned embryos at the 8-cell and blastocyst stages of preimplantation development   总被引：2，自引：0，他引：2  

Amarnath D  Li X  Kato Y  Tsunoda Y 《The Journal of reproduction and development》2007,53(6):1247-1263

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Anti-hypertensive substances in fermented soybean,natto   总被引：17，自引：0，他引：17  

Akiko Okamoto  Hiroshi Hanagata  Yukio Kawamura  Fujiharu Yanagida 《Plant foods for human nutrition (Dordrecht, Netherlands)》1995,47(1):39-47

Natto is a traditional Japenese fermented food made by fermenting boiled soy beans withBacillus natto. Its contents of inhibitors against the angiotensin converting enzyme (ACE, EC3.4.15.1) were investigated. Relatively strong inhibitory activity (IC₅₀:0.4 mg/ml, 11.8 inhibition units/g natto) was detected in natto extracts and the inhibitory activity observed in the viscous fraction was more potent than in the bean extract. Two groups of inhibitors in the viscous material, high and low molecular weight inhibitors, were resolved by dialysis test. The inhibitor of high molecular weight was a protein with low IC₅₀ value (0.12 mg/ml). The two types of low molecular weight inhibitors were detected in ethanol extracts (IC₅₀: 0.53 mg/ml and 0.95 mg/ml) and they were found to be stable over a wide range of pH and temperature up to 100°C. They were different in the mode of ACE inhibition. One is competitive, and the other noncompetitive against the hydrolysis of Bz-Gly-His-Leu by ACE.  相似文献   

Induction of haploid plants by anther culture in tuber-bearing species and interspecific hybrids ofSolanum     

Yukio Irikura 《Potato Research》1975,18(1):133-140

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