Effect of Dietary Lactobacillus fermentum from Pig on Growth Performance,Nutrient Apparent Digestibility,Faecal Microflora Number and Serum Immune Indices in Weaned Piglets     

WANG Si-xin  JI Hai-feng  LIU Hui  ZHANG Dong-yan  WANG Jing  WANG Ya-min  DONG Da-ming 《中国畜牧兽医》2016,43(5):1215-1220

This experiment was conducted to study the effects of Lactobacillus fermentum on growth performance, nutrient apparent digestibility, faecal microflora number and serum immune indices of weaned piglets.A total of 160 weaned piglets with an average age of (30±2) days and averge body weight of (7.85±0.68) kg were randomly distributed into 4 groups with 4 replicates per group and 10 piglets per replicate feeding basal diet, 0.1%, 0.2%, and 0.3% Lactobacillus fermentum supplementation diets, respectively.The experiment lasted for 35 d.The results showed that, compared with the control group, the average daily gain, the feed conversion ratio, the apparent digestibility of crude protein, calcium and total phosphorusin of Lactobacillus fermentum groups were all increased in varying degrees.The optimum supplemental level of Lactobacillus fermentum should be 0.2%, and at this level, the average daily gain was significantly increased by 8.70% (P<0.05), the F/G was decreased by 4.62% (P<0.05), the apparent digestibility of crude protein, calcium and total phosphorus were significantly increased (P<0.05), the number of faecal E.coli was significantly decreased (P<0.05), and the serum IgG was significantly increased by 8.39% (P<0.05).The results indicated that Lactobacillus fermentum supplementation could improve the immune function, growth performance, the apparent digestibility of crude protein, calcium and total phosphorus and reduce the number of faecal E.coli of weaned piglets.  相似文献   

小麦面粉中赭曲霉毒素A的CdSe/ZnS荧光量子点免疫探针检测     

齐佳  张文刚  赵静静  史茂敏  李忠宏 《西北农业学报》2015,24(4):56-51

构建一种基于荧光量子点免疫探针的小麦面粉中赭曲霉毒素A(OTA)的检测方法。以EDC为活化剂将CdSe/ZnS半导体荧光量子点与OTA单克隆抗体偶联,制备OTA检测探针并评价其检测性能。结果表明:制备的检测探针在3d保存期内具有良好的稳定性。当OTA质量浓度为0.1~0.9μg/L时,检测探针的荧光强度与OTA质量浓度呈线性关系,其相关系数为0.996 3,检出限为0.017 7μg/L。与国标法对照的结果说明,此方法具有可行性。小麦面粉中的加标回收试验结果显示,OTA的回收率为96.0%~102.7%,相对标准偏差不超过4.1%,说明准确性良好。可见,OTA的CdSe/ZnS荧光量子点免疫探针检测方法操作简单、准确度高、结果稳定,有望应用于小麦面粉中OTA的检测。  相似文献   

保定地区几个早熟苹果品种(系)生长观察     

白旭亮  李方方  陈晓洁  郭兴科  张学英  李中勇  徐继忠 《河南农业科学》2015,(4)

为筛选适宜保定地区栽植的早熟苹果品种,以嫁接在SH40中间砧上的不同早熟苹果品种(系)为试材,测定了不同品种(系)早熟苹果树体生长发育指标和果实品质。结果表明:除嘎啦早熟变异外,其他供试品种(系)树体生长较好,3年生时Xu2-7、Xu3-2树高330.0~352.5 cm,4年生时为394.0~417.5 cm,Xu3-2、藤牧1号、信浓红4年生为336.5~381.0 cm。4年生时,干径以Xu2-5最大;短枝、中枝、叶丛枝均以Xu2-5最多,长枝以Xu2-7最多,总枝量以Xu2-5最多;藤牧1号短枝所占比例最高(57.4%)。应用模糊综合评判法得出Xu2-5、Xu2-7、信浓红、藤牧1号果实品质较高。  相似文献   

MdMYB73的分子克隆及其在苹果愈伤组织和拟南芥幼苗中的盐抗性功能鉴定     

张全艳  刘晓  于建强  胡大刚  郝玉金 《园艺学报》2016,43(11):2073-2078

从‘嘎拉’苹果中克隆了一个MYB转录因子基因(序列号:MDP0000894463)。该基因包含长为729 bp完整的开放阅读框,编码243个氨基酸,预测其蛋白质分子量为26.34 kD,等电点为9.29。系统进化树分析表明,这一MYB转录因子与拟南芥AtMYB73同源序列相似性最高,因此将其命名为MdMYB73。功能域分析表明,MdMYB73蛋白含有保守的R2R3-typeMYB绑定域。荧光定量PCR分析表明,MdMYB73在苹果的各个组织均有表达,在叶片和花中表达相对较高;MdMYB73的表达明显受盐胁迫的诱导。将异位表达MdMYB73的拟南芥幼苗进行抗盐鉴定,结果表明MdMYB73负调控拟南芥盐胁迫抗性;同时,AtSOS1,AtSOS3和AtNHX1抗盐相关基因的表达水平显著降低,表明MdMYB73可能负调控SOS反应,影响拟南芥抵抗高盐胁迫过程。将MdMYB73基因遗传转化苹果愈伤组织,抗盐表型分析表明,MdMYB73过量表达也明显降低了转基因愈伤组织对盐胁迫的抗性。  相似文献   

养殖废弃物生物有机肥料对藜蒿产量及肥料利用率的影响     

张建  蒋细旺 《长江蔬菜》2016,(4):60-65

研究了养殖废弃物生物有机肥料和化肥配施与单施化肥对云南藜蒿产量、相对干物质含量、土壤养分含量及肥料利用率的影响。试验结果表明,各施肥处理的藜蒿产量(21 273~29 110 kg/hm~2)均显著高于对照(17 765 kg/hm~2),其中有机无机肥配施处理的藜蒿产量(20 334~29 110 kg/hm~2)比单施化肥处理(21 273 kg/hm~2)增加19.8%~36.8%;同时,有机无机肥配施处理的藜蒿相对干物质含量比单施化肥处理提高了1.14%~5.48%;与单施化肥处理相比,有机无机肥配施处理明显提高了土壤养分含量、改善了土壤供肥特性、提高了肥料利用率;藜蒿地上部的养分积累量、肥料回收率和肥料农学利用效率及生理利用效率均显著提高。  相似文献   

Whole-genome identiifcation and expression analysis of K+eflfux antiporter (KEA) and Na+/H+antiporter (NHX) families under abiotic stress in soybean     

CHEN Hua-tao  CHEN Xin  WU Bing-yue  YUAN Xing-xing  ZHANG Hong-mei  CUI Xiao-yan  LIU Xiao-qing 《中国农业科学(英文版)》2015,(6)

Sodium toxicity and potassium insufifcient are important factors affecting the growth and development of soybean in saline soil. As the capacity of plants to maintain a high cytosolic, K+/Na+ratio is t...  相似文献   

Effect of AIR on Inflammatory Reaction Infected by Brucella     

XU Ya-fang  CHEN Chuang-fu  WANG Hao  FU Qiang  SHI Hui-jun  SUN Zhi-hua  ZHANG Hui  GUO Fei 《中国畜牧兽医》2016,43(6):1437-1445

In order to investigate the effect of AIR on inflammatory reaction infected by Brucellamelitensis (16M), the AIR domain of Tecpr1 gene of murine macrophages RAW264.7 were knocked down (I-A), overexpressed (O-A) and reversed (OA-IA). Using the chlorine fluorescein (DCFH-DA) as a probe, we detected the variation of ROS production and mitochondria distribution by confocal laser scanning microscopy. We observed the expression changes of NLRP3, ASC and Caspase-1 by qRT-PCR and the expression changes of IL-18,IL-1β and Caspase-1 in host cells by ELISA. The results showed that 16M could stimulate RAW264.7 cells to produce ROS by time-dependent pathway, and I-A group and O-A group showed more abnormal accumulation of mitochondrial. The results of qRT-PCR and ELISA suggested that it had effect on the expression levels of NLRP3, ASC,Caspase-1 and IL-18, IL-1β and Caspase-1 in cells of different groups. Those results indicated that with AIR gene deletion, the release amount of ROS changed, mitochondrial clustered abnormally, and AIR was closely related to the activation of inflammasomes and induction of inflammatory reactions.  相似文献   

表达鸡α干扰素重组酿酒酵母菌的构建与鉴定     

杜伟立  尹美辰  杨理凯  张涛  王令  路宏朝 《动物医学进展》2017,38(9)

通过构建鸡α干扰素真核表达载体,以获得能够表达鸡α干扰素的酵母工程菌株。首先根据GenBank公布的鸡α干扰素基因序列设计上、下游引物,以略阳乌鸡基因组DNA为模板,PCR扩增鸡α干扰素基因片段。然后,利用基因重组技术将鸡α-干扰素基因插入真核表达载体,通过菌落PCR和DNA测序确定目标载体,构建成功后转化至酿酒酵母AH109,再通过营养缺陷培养、质粒提取和PCR鉴定带JMB440-ChIFN-α载体的重组酵母菌株。最终通过酵母培养、总蛋白收集、SDS-PAGE电泳和Western blot等,确定鸡α干扰素基因能够与酵母菌株重组,并表达α干扰素,蛋白条带大小约为23ku,与预期结果一致,表明成功构建了表达鸡干扰素基因的工程酵母菌株。  相似文献   

2012年春季塔克拉玛干沙漠腹地沙尘颗粒的组成研究     

张延伟  唐湘玲  陆辉  葛全胜  王雯雯  魏文寿 《干旱区资源与环境》2017,31(11):94-99

为研究塔克拉玛干沙漠腹地沙尘颗粒的微观形貌及其矿物、元素组成,应用扫描电镜能谱技术(SEM/EDX)对塔克拉玛干沙漠腹地塔中观测站2012年5月采集的PM10样品进行了识别和分析。统计622个单颗粒高分辨电镜图像及能谱的结果表明:塔克拉玛干沙漠大气PM10主要来源于地壳矿物。其中,矿物组成主要是石英、粘土矿物、方解石、岩盐,特别是岩盐含量高达14.5%,石英、方解石、石膏、岩盐单体矿物相对含量较高,但大部分都以矿物集合体的形式存在,与其它人为源颗粒物的混合主要是外混合。不同天气类型下,其矿物组成差异明显,重矿物(岩盐等)主要来源于强风就地扬起或携带的较粗颗粒。  相似文献   

舟山长白岛民俗文化探析     

张洁 《安徽农业科学》2015,(6):206-207,232

以长白岛为例,通过对长白岛民俗文化的现状分析,发现其存在的问题,由此提出保护和发展长白岛民俗文化的建议和措施,希望对长白岛的文化、经济、旅游业发展具有一定的借鉴意义.  相似文献