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81.
de Azevedo Pereira R Valencia-Jiménez A Magalhães CP Prates MV Melo JA de Lima LM de Sales MP Tempel Nakasu EY da Silva MC Grossi-de-Sá MF 《Journal of agricultural and food chemistry》2007,55(26):10714-10719
The coffee berry borer, Hypothenemus hampei (Ferrari), is an important devastating coffee pest worldwide. Both trypsin and chymotrypsin enzyme activities from H. hampei larval midgut can be inactivated by proteinaceous enzyme-inhibitors. A serine proteinase inhibitor belonging to the Bowman-Birk class was purified from a wild accession of Phaseolus coccineus L. seeds. The inhibitor (PcBBI1) is a cysteine-rich protein that is heat-stable at alkaline pH. MALDI-TOF/MS analysis showed that PcBBI1 occurs in seeds as a monomer (8689 Da) or dimer (17,378 Da). Using in vitro inhibition assays, it was found that PcBBI1 has a high inhibitory activity against H. hampei trypsin-like enzymes, bovine pancreatic chymotrypsin, and trypsin. According to this, PcBBI1 could be a promising tool to make genetically modified coffee with resistance to coffee berry borer. 相似文献
82.
83.
Elena Palacios Ilie S. Racotta Horacio Heras Yanic Marty Jeanne Moal Jean-François Samain 《Aquaculture International》2001,9(6):531-543
In this study, lipid classes and fatty acid composition were determinedin eggs of P. vannamei as a function of survival to zoeaIII stage. Spawns were reared individually to zoea III and grouped, accordingtotheir final survival to this larval stage, into spawns of high and lowsurvival.Eggs of individual spawns were analyzed for lipid and fatty acid composition ofneutral and polar lipids and the results were then grouped according tosurvivalto zoea III. The lipids within each group (high and low survival to zoea III)were pooled for the separation of phospholipids and the analysis of their fattyacid composition.Higher levels of triglycerides, carotenoids, and linoleic (18:2n-6) acid ineggs were associated with improved survival to zoea III. Linoleic acid washigher in spawns from the high survival group in both neutral and polarfractions and in most of the phospholipid classes analyzed. Docosahexanoic acid(DHA) was not related to survival to zoea, probably because its content washighenough (> 15% of total fatty acids) to satisfy embryo and early larvaldevelopment needs. A high content of eicosapentaenoic acid (EPA) was found inphosphatidylethanolamine, lysophosphatidylethanolamine, phosphatidylserine, andphosphatidylinositol compared to other phospholipid classes, suggesting aspecific role of EPA in these lipids. These results describe the specificphospholipid composition of penaeid eggs and could (potentially) be used aspredictive indicators of larval quality for research and production purposes. 相似文献
84.
85.
Effects of road transportation or droving on the weight and metabolism of young bulls 总被引:1,自引:0,他引:1
Marcella Cândia D’Oliveira Maria Inês Lenz Souza Ruy Alberto Caetano Corrêa Filho Maria da Graça Morais Camila Celeste Brandão Ferreira Ítavo Gumercindo Loriano Franco 《Tropical animal health and production》2014,46(8):1447-1453
This study evaluated the effects of the mode of transportation on weight and metabolism of young bulls, which are generally transported by two systems: droving and truck. This experiment consisted of two phases: transportation and refeeding and involved transportation by droving and truck. Fifty young Nellore bulls were separated in equal numbers in both groups, droving group and truck group. In the transportation phase, animals were moved, in a 640-km journey. The refeeding phase consisted of 84 days and commenced after the animals arrived at the fattening farm. The effect of the transportation system on animals’ weight, metabolic hormones, and serum biochemistry was assessed after 28, 56, and 84 days. During the transportation phase, animals in the droving group lost 10.11 kg and animals in the truck group gained 13.1 kg. The truck group showed 20.5 kg of weight gain at the end of the refeeding phase. The highest triiodothyronine (T3) and thyroxine (T4) concentrations were observed on days 0 and 60 in the transportation phase in both droving and truck groups. The serum cortisol concentration was higher in the droving group than in the truck group on day 56 of the refeeding phase. The IGF-I concentrations were higher during the refeeding phase in the droving group than in the truck group. Our results showed that transportation by droving leads to increased weight loss and reduced further weight gain compared to transportation by truck and that serum concentrations of T4, cortisol, and insulin-like growth factor (IGF-I) hormones are altered by the transportation system. 相似文献
86.
Jessica Maria Leite dos Santos Jomar Patrício Monteiro Wesley Lyeverton Correia Ribeiro Iara Tersia Freitas Macedo Ana Lourdes Fernandes Camurça-Vasconcelos Luiz da Silva Vieira Claudia Maria Leal Bevilaqua 《Veterinary parasitology》2014,199(3-4):160-164
Haemonchus contortus is the most prevalent nematode in Brazil. The objective of this study was to select 6 populations of H. contortus of known or suspected benzimidazole resistance status and characterize these using quantitative real-time polymerase chain reaction (qPCR) for single nucleotide polymorphisms (SNPs) F200Y, F167Y and E198A in the β-tubulin isotype 1 gene. qPCR was performed using DNA from a pool of 10 adult male H. contortus from a single animal per farm. Faecal egg count reduction test (FECRT) and egg hatch test (EHT) were used to determine the resistance status. Samples were obtained from 6 farms located in 5 counties in the Ceará State: Tauá, Boa Viagem, Quixadá, Santa Quitéria and Solonópole. The inbred-susceptible-Edinburgh (ISE) isolate was used as reference for comparative purposes in the qPCR. Benzimidazole resistance was detected by FECRT on all farms with efficacy values ranging from 0 to 51%. EC50 values as determined by EHT were all above 1.49 μg/ml. High frequencies of the resistant SNPs F200Y and F167Y alleles were detected but no resistance was detected at SNP E198A. Our results suggest that the SNPs F167Y and F200Y are both important for benzimidazole resistance in the studied populations. 相似文献
87.
I. François F.‐X. Lalèyê M. Micat K. Benredouane K. Portier 《Equine veterinary journal》2014,46(5):596-600
88.
?zge Turna YILMAZ T. Seval Fatma TOYDEMIR ?smail KIR?AN Banu DOKUZEYLUL Zeynep GUNAY Esra KARACAM 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(12):1595-1601
The analgesic effect of wound
infiltration with bupivacaine was evaluated in cats undergoing bilateral mastectomy.
Twenty-one female cats with mammary gland tumors were anesthetized with propofol and
oxygen-isoflurane anesthesia following premedication with atropine. In the trial group
(Group I; n=11), 30 ml of saline containing 2 mg/kg of bupivacaine was
infiltrated topically into the surgical wound right after removal of the mammary glands,
whereas only saline solution was infiltrated in the control group (Group II; n=10). At the
same time, carprofen (4 mg/kg) was also administered subcutaneously in both groups.
Behavioral signs of pain were monitored during the recovery period after general
anesthesia. In order to examine the behavioral changes associated with acute pain, a
questionnaire was prepared and given to the owners to be completed 4 hr and then 10 hr
after the operation. According to the owners’ anwers to the questionnaire, a pain score
was specified using a “numerical rating scale” for each cat. Although some cats showed
mild to moderate pain, the pain score recorded at 4 hr after the operation was
significantly lower in Group I (P<0.001). No significant difference
was found at 10 hr after the operation between the groups. The incidence of vocalization,
aggression and convulsion within 2 hr after the operation was also lower in Group I. In
conclusion, wound infiltration with bupivacaine before incisional closure provided
reliable analgesia at least 4 hr after bilateral radical mastectomy in cats. 相似文献
89.
A. L. Lourenço A. Dias‐da‐Silva A. S. Santos M. A. M. Rodrigues J. W. Cone L. M. M. Ferreira 《Journal of animal physiology and animal nutrition》2014,98(3):453-457
The digestive capacity of the Portuguese native breed of Barrosão cattle and the Holstein–Friesian breed was measured when fed meadow hay (72–74 g CP and 641–671 g NDF/kg dry matter), offered either alone or supplemented with soya bean meal (150 g/kg dry matter), at maintenance level. Four mature cows of each breed were used. Average initial live weight (LW) was 457 and 635 kg for the Barrosão and the Holstein–Friesian cows respectively. The organic matter digestibility (OMD) of the meadow hay was higher in Holstein–Friesian than in Barrosão cows (p < 0.08) whether supplemented or not. The neutral detergent fibre digestibility of the hay was also higher in Holstein–Frisian when the hay was fed alone (p < 0.08). The soya bean meal supplementation increased the OMD and the NDFD digestibility of the total diet (p < 0.05), but not the OMD digestibility of the hay (p > 0.05). The results obtained in this study suggest a higher ability to digest fibre in the large dairy breed than in the small native breed. This suggestion is also supported by previous findings with sheep breeds largely differing in mature live weight. 相似文献
90.