Effects of surgically implanted dummy ultrasonic transmitters on physiological response of bighead carp Hypophthalmichthys nobilis     

Hongwei Luo  Xinbin Duan  Shaoping Liu  Daqing Chen 《Fish physiology and biochemistry》2014,40(5):1521-1532

The study assessed the effects of surgically implanted dummy ultrasonic transmitters on physiological response of bighead carp Hypophthalmichthys nobilis in April 2011. Before the surgery, 15 blood samples were extracted randomly from 195 bighead carp samples, and then the rest of the fish were divided into three groups: (1) control group, handing but no tagging, (2) sham group, surgical procedure without implantation of transmitter and (3) surgery group, surgical implantation of transmitters. In 3 h, 24 h, 7 days and 14 days after surgery, 15 fish were extracted randomly from the three groups, respectively, for sampling. Then the plasma samples were analyzed, and physiological measures of stress response (cortisol, glucose), tissue damage [alanine aminotransferase (ALT) and aspartate aminotransferase (AST)] and nutritional status [total protein, globulin, albumin, triglyceride, cholesterol, and alkaline phosphatase (ALP)] were compared. The result showed that there was no significant difference between sham and surgery groups in 3 h, 24 h, 7 days and 14 days after surgery. When compared to the control group, there were significant increases in concentrations of plasma cortisol, glucose, ALT, AST, total protein and globulin of sham and surgery groups in 3 h after surgery. After 24 h, the levels of plasma cortisol, ALT, AST, total protein, globulin and ALP were elevated in both sham and surgery groups, whereas the levels of plasma glucose had declined to normal level and plasma albumin, cholesterol and triglyceride were significantly decreased in both sham and surgery groups. After 7 days, the levels of plasma glucose, albumin and cholesterol continued to decline, while the level of plasma ALT, globulin and ALP had declined but still remained higher for sham and surgery groups than control group; however, the plasma total protein level had returned to normal. After 14 days, there was no significant difference between the three groups. The above results showed that surgical implantation of ultrasonic transmitters had indeed caused significantly negative effects on the physiological response of bighead carp. However, all the negative influences on stress response, tissue damage and nutritional status had disappeared in 14 days after surgery, and the presence of transmitters had no significant negative impact. Therefore, the bighead carp should be temporary cultured at least 14 days for the recovery of physiological response and then released into the free water for the ultrasonic telemetry research.  相似文献   

Effects of glycinin on growth performance,immunity and antioxidant capacity in juvenile golden crucian carp,Cyprinus carpio × Carassius auratus     

Min Li  Liang Li  Yi‐Di Kong  Rui Zhu  Zhe Yu  Jing‐Yao Wang  Jing Duan  Li‐Fang Wu 《Aquaculture Research》2020,51(2):465-479

This study was designed to examine the effects of glycinin on growth, digestive ability, immune responses, antioxidant capacity and gene expression levels of golden crucian carp. Golden crucian carp were fed diets containing glycinin at 0, 30, 60, 90 and 120 g/kg, respectively, for 8 weeks. Body weight, weight gain percentage, specific growth rate and feed efficiency ratio were negatively related to the content of glycinin in diet. Activities of protease, acid phosphatase, alkaline phosphatase, lysozyme in hepatopancreas, and activities of catalase, glutathione peroxidase, superoxide dismutase, and total antioxidant capacity in the proximal intestine, mid intestine, distal intestine and hepatopancreas were negatively related to the content of glycinin in diet, whereas malondialdehyde in proximal intestine, mid intestine, distal intestine and hepatopancreas increased directly with the content of glycinin in diet. Furthermore, the relative expressions of TNF‐α and IL‐1β in proximal intestine, mid intestine and distal intestine increased directly with the content of glycinin in diet, whereas the relative expressions of TNF‐α and IL‐1β in hepatopancreas were negatively related to the content of glycinin in diets. The relative expressions of IL‐10 in proximal intestine, mid intestine, distal intestine and hepatopancreas all were negatively related to the content of glycinin in diets. In conclusion, reductions in growth, immunity and antioxidant capacity, intestine inflammation with dysfunction of digestive system occurred in golden crucian carp that fed a diet containing glycinin at 30 g/kg or higher after 8 weeks.  相似文献   

优质抗寒、抗病酿造葡萄新品种‘北红’     

范培格  黎盛臣  王利军  杨美容  吴本宏  李连生  钟静懿  张映祝  文丽珠  张凤琴  罗方梅  段伟  李绍华 《园艺学报》2009,36(7):1085-1085

 ‘北红’葡萄新品种由‘玫瑰香’与‘山葡萄’杂交育成。浆果在北京地区9月底成熟。果粒圆形,蓝黑色,果粒质量1.57g,果穗质量160.0 g,浆果可溶性固形物含量23.8 %～ 27.0 %,含酸量0.89～1.26 %。早果性及丰产性强。抗寒、抗病能力特强。酿成的酒深宝石红色,酒体平衡,酒质上等。  相似文献   

竹醋液对七种食用菌菌丝生长的影响   总被引：1，自引：0，他引：1  

李阳  刘德云  宋小亚  郑巧平  顾新伟  王正郁 《食用菌学报》2009,16(1)

采用菌丝生长速率法研究了竹醋液对7种食用菌菌丝生长的影响.结果表明,竹醋液在供试浓度下对金针菇(Flammulina velutipes)、杏鲍菇(Pleurotus eryngii)和双孢蘑菇(Agaricus bisporus)的菌丝生长均具有显著抑制作用,最高抑制率可达100%;而对秀珍菇(P.geesteranus)、黑木耳(Auricularia auricula)、香菇(Lentinula edodes)和灰树花(Grifola frondosa)菌丝生长,则在较高供试浓度条件下表现强烈的抑制作用,在低供试浓度条件下表现显著促进作用.  相似文献   

苹果密植园修剪存在的问题及解决途径     

段应菊 《中国果菜》2009,(2):33-33

上世纪九十年代中期洛宁发展的苹果园大多果园采用了3×5米中密栽培,有部分矮化果园采用了3×4m,矮化树短枝型品种多为2×4m或2×3m,亩栽44—100株极为普遍。但是在生产上多数还沿有过去乔化稀植的传统整形与修剪方法,造成部分树生长过旺,条子太多,养分浪费较大,树冠交叉郁闭,通风透光不好,上强下弱,果品质量差,落叶病及虫害难以防治,致密植园既不能早产也不能丰产。我们对密植园在修剪中存在的问题进行了调研,探索出了一套解决问题的办法,在实践上取得了良好效果。  相似文献   

我国秸秆人造板工业的发展现状及前景展望   总被引：2，自引：0，他引：2  

段海燕  贺小翠  尚大军  杨中平 《农机化研究》2009,31(5)

分析了我国发展农作物秸秆人造板工业的必要性和可行性,概述了我国秸秆人造板工业的发展现状及发展过程中应注意的问题,并对我国秸秆人造板工业的发展前景进行了分析和展望,认为我国的秸秆人造板工业发展前景广阔,并将进入一个快速发展时期.  相似文献   

施用生防颗粒剂对大豆田土壤线虫群落结构和生物多样性的影响   总被引：7，自引：3，他引：7  

陈立杰  梁文举  段玉玺  栾军波  李琪 《大豆科学》2003,22(4):251-256

针对大豆生长期内土壤线虫的变化，利用自行研制的生防颗粒剂豆丰一号对大豆田土壤线虫的群落结构和生物多样性进行了研究，共鉴定出5目14科的土壤线虫，其中植物寄生线虫是大豆根际土壤线虫的优势营养类群。与对照相比，生防颗粒剂处理在大豆的整个生育期抑制了土壤线虫总量和植物寄生线虫群体数量，但在大豆生育末期促进了食细茵线虫和杂食—捕食性营养类群的增加。应用多样性指数H’、成熟度指数MI、均匀度指数J’、丰富度指数SR、优势度指数l和植物寄生线虫成熟度指数PPI分析比较了处理区和对照区土壤线虫生物多样性变化规律，其中，多样性指数H’、丰富度指数SR和成熟度指数MI较好的反映了生防颗粒剂的施用对大豆田土壤线虫生物多样性的促进作用。  相似文献   

油松大蚜的种群动态、有效积温及防治     

邵东华  段立清  段景攀 《东北林业大学学报》2017,45(7)

通过野外调查和室内不同温度下饲养观察,对油松大蚜(Cinara formosana)生活史、种群动态、有效积温、天敌、物候预测和化学防治进行了研究。结果表明:油松大蚜1年可发生8代,6月中旬分化出有翅孤雌蚜并扩散,10月上旬性蚜交配产卵,以卵越冬;油松大蚜种群数量1年内有2个高峰期,分别在6月上旬和9月上旬,8月上旬—8月中旬种群数量最低;油松大蚜不同虫态的发生与山桃(Prunus davidiana)、连翘(Forsythia suspense)、龙爪槐(Sophora japonica)和火炬树(Rhus typhina)的一些物候相吻合,可通过观察这些植物的物候来预测油松大蚜各虫态发生情况;油松大蚜优势天敌依次为异色瓢虫(Leis axyridis)、七星瓢虫(Coccinella septempunctata)、蚜茧蜂(未鉴定);油松大蚜4个龄期的若虫及全世代的发育起点温度分别为4.5、6.7、5.9、7.7、3.1℃,有效积温分别为48.6、49.3、67.4、62.7、291.7日·度;胶带-药膜法防治油松大蚜效果显著,14 d后处理区校正虫口减退率可达80%以上。  相似文献   

不同施磷方式对海岛棉农艺性状及产量的影响     

刘正兴  郭仁松  段雅洁 《棉花科学》2020,42(2):32-37

在新疆生态条件下,采用海岛棉新海35号研究同等用量3种施磷方式(犁地前全部撒施、犁地前撒施50%与作种肥施50%和全部作种肥施)对棉花农艺性状及产量的影响。结果表明:施磷方式对棉花生育进程的影响较小,各处理对新海35号棉花的农艺性状没有显著性的影响,P1处理可略为提高棉花花铃期叶面积指数,其中P1处理会略提高棉花后期的干物质积累量,以P1、P2处理的方式施磷肥,棉花的单株结铃数可提高4%~7%皮棉产量可提高12%~14.8%。  相似文献   

The dynamics of bacterial community in a polyculture aquaculture system of Penaeus chinensis,Rhopilema esculenta and Sinonovacula constricta     

Xiaoyan Guan  Bai Wang  Ping Duan  Jiashen Tian  Ying Dong  Jingwei Jiang  Bing Sun  Zunchun Zhou 《Aquaculture Research》2020,51(5):1789-1800

Polyculture with Chinese shrimp Penaeus chinensis, jellyfish Rhopilema esculenta and razor clam Sinonovacula constricta (S.J.R) is a common aquaculture system in Dandong, China. Investigation on the composition and function of microbial communities and their relationship with environmental factors in aquaculture systems have crucial implications. Therefore, the sequencing of 16S rRNA gene was applied to profile the bacterial community composition of water and sediment in S.J.R polyculture system in the present study. Proteobacteria was the dominant phylum in S.J.R polyculture ponds following with Actinobacteria and Bacteroidetes in water; in contrast, more diverse phyla were detected in sediments. Water temperature, pH and dissolved oxygen were determined the bacterial community composition in the S.J.R polyculture systems. Specially, Planktomarina, PS1 clade, Candidatus Aquiluna and Fibrobacteres, which can resist the phytoplankton blooms or degrade the cellulose, were more abundant in water at the early stage of cultivation in the S.J.R polyculture system. Moreover, the functions of bacterial communities in water were mainly associated with cell communication mediated, while the functions related to basal metabolism were enriched in sediments. The findings of this study allowed us to better understand the bacterial community in the S.J.R polyculture system and accumulated data for the improvement of cultural management.  相似文献