鸡FKBP5基因的克隆、生物信息学及组织表达谱分析     

唐贺贺  张真真  张献珍  蒋瑞瑞  李国喜  田亚东  康相涛  韩瑞丽 《中国畜牧兽医》2021,(1)

本研究旨在对鸡FK506结合蛋白5(FK506 binding protein 5,FKBP5)基因进行克隆和生物信息学分析,并检测其在鸡不同组织中的表达量。以鸡脾脏cDNA为模板,通过PCR方法扩增和克隆鸡FKBP5基因完整CDS区序列,并进行同源性比对及系统进化树构建;使用在线软件分析FKBP5蛋白的理化性质、疏水性、跨膜结构、信号肽、二级结构和三级结构;利用实时荧光定量PCR检测其在肢体内外翻畸形(valgus-varus deformity,VVD)组肉鸡和正常组肉鸡组织中的表达量,并绘制组织表达谱。结果显示,鸡FKBP5基因CDS区序列全长1350 bp,编码449个氨基酸;同源性比对和进化树分析表明,鸡FKBP5基因氨基酸序列与鹌鹑、鸭、壁虎、中华鳖、小鼠、人、猪、斑马鱼的同源性分别为98.4%、96.2%、85.8%、87.4%、81.1%、85.2%、86.1%和61.2%,与鹌鹑、鸭的亲缘关系最近,爬行类和哺乳类动物次之,与斑马鱼(鱼类)亲缘关系最远。鸡FKBP5蛋白分子质量为50.43 ku,理论等电点(pI)为5.94,半衰期为30 h,肽链N端为蛋氨酸(Met),不稳定系数为23.80,属于稳定蛋白;跨膜区和信号肽预测结果显示,FKBP5蛋白不属于跨膜蛋白和分泌型蛋白。结构域分析结果表明,该蛋白包括2个FKBP型肽基脯氨酰异构酶(FKBP-type peptidylprolyl isomerases)、3个四肽重复(TPR)序列,主要包含α-螺旋(46.77%)、延伸链(12.47%)和无规则卷曲(40.76%),且该蛋白与HSPA2、PPID、STIP1、HSP90AA1和HSP90AB1等互作蛋白具有很强的相关性。实时荧光定量PCR结果显示,FKBP5基因在鸡各组织中广泛表达,其中在软骨和法氏囊中表达量较高,在发病组肉鸡组织中的表达量均高于正常组肉鸡,且在心脏、腿肌、法氏囊、胸腺、软骨中表达量差异显著(P<0.05),在脾脏中表达量差异极显著(P<0.01)。本试验结果可为肉鸡骨骼疾病及腿部健康选育提供参考。  相似文献   

不同来源纤维饲粮对福建黄兔表观消化率、十二指肠组织形态及盲肠纤维消化酶活性的影响     

张丽萍  王建烽  李真真  雷琼  党浩千  刘庆华 《中国畜牧兽医》2021,48(2):501-508

本试验旨在研究不同来源纤维饲粮对福建黄兔表观消化率、十二指肠组织形态及盲肠纤维消化酶活性的影响。试验选用200只健康、体重接近的断奶福建黄兔,随机分为4组,每组5个重复,每个重复10只。4组分别饲喂由添加比例为25%的苜蓿草粉、甜菜渣、燕麦草、大麦糠为纤维饲料原料配制而成的饲粮。试验期为60 d,于试验第54天以全收粪法进行消化试验,屠宰后测定十二指肠绒毛高度、隐窝深度及二者比值,盲肠纤维素酶、半纤维素酶和果胶酶活性。结果表明：①苜蓿草粉与甜菜渣组的中性洗涤纤维（NDF）、酸性洗涤纤维（ADF）、不溶性纤维（IDF）、可溶性纤维（SDF）的消化率均极显著高于大麦糠与燕麦草组（P<0.01）。其中,甜菜渣组不溶性纤维、可溶性纤维的消化率最高,与苜蓿草粉组差异显著（P<0.05）。②苜蓿草粉与甜菜渣组的绒毛高度极显著高于大麦糠组（P<0.01）,但显著低于燕麦草组（P<0.05）,隐窝深度显著低于大麦糠和燕麦草组（P<0.05）,其中甜菜渣组隐窝深度最低。甜菜渣组的绒毛高度/隐窝深度（V/C）最高,极显著高于其他组（P<0.01）。③苜蓿草粉与甜菜渣组盲肠3种纤维消化酶活性均极显著高于大麦糠和燕麦草组（P<0.01）,其中甜菜渣组的3种酶活均极显著高于苜蓿草粉组（P<0.01）。综上所述,对比4种纤维饲粮,在饲粮配方中添加25%的甜菜渣能提高肉兔的表观消化率与盲肠纤维消化酶活性,并能改善十二指肠组织形态。  相似文献   

我国农业机械化与城镇化关系的协整分析     

侯方安  李斯华 《中国农机化学报》2015,(2):300-304

在讨论我国农业机械化发展的内在机制以及与城镇化关系的基础上,运用协整分析方法建立时间序列数据模型,经过格兰杰因果关系检验,验证农业机械化与城镇化之间的长期稳定关系,进一步建立误差修正模型探讨这种稳定关系在长期内与短期内的动态变化特征,并预测我国2020年农业机械化发展水平。结果表明,在长期内,城镇化率增加一个单位农业机械化水平将提高1.09个单位。但是,农业机械化对当期城镇化的推进反应是滞后的,而是反映在长期趋势上。预测显示,2020年我国农业机械化水平将达到67.45%。  相似文献   

沼液追肥对制种玉米产量与土壤化学性质的影响     

鲁天文  王勤礼  许耀照  吕彪  闫芳 《中国沼气》2015,(2):81-83

研究沼液作追肥对制种玉米产量及土壤化学性质的影响。文章以制种玉米为试验材料,采用计随机区组试验设,研究沼液追肥对制种玉米经济性状、产量、土壤化学性质、经济效益的影响。结果表明,T1(沼液1500kg·hm-2+142.50 kg N·hm-2)有机质含量最高,与其他处理间达到了显著差异;T1的土壤速效钾含量最高,达12.67 g·kg-1,与CK(150 kg N·hm-2),T2(沼液22500 kg·hm-2+138.75 kg N·hm-2)间达到了显著差异;土壤速效磷含量,土壤p H值,土壤电导率差异不显著;不同处理对穗行数,行粒数、穗粒重的影响差异不显著,T3(沼液30000 kg·hm-2+135 kg N·hm-2)千粒重最高,与CK,T2,T4(沼液37500 kg·hm-2+131.25 kg N·hm-2)间差异达到了显著水平;T2的产量,产值,经济效益最高。沼液做追肥可提高产量,降低生产成本,但必须和化肥配合施用。  相似文献   

Effects of dietary lysozyme levels on growth performance,intestinal morphology,non‐specific immunity and mRNA expression in weanling piglets          下载免费PDF全文

Jiatao Zhu  Xiaoxue Pang  Zhengfeng Fang  Yan Lin  Lianqiang Che  Shengyu Xu  Jian Li  Yiming Huang  Xiang Su  De Wu 《Animal Science Journal》2016,87(3):411-418

The aim of the present study was to determine the effect of dietary lysozyme levels on growth performance, gut health and non‐specific immunity of weanling piglets. A total of 150 weanling piglets were allocated to six treatments. The piglets were fed the same basel diet supplemented with 0, 30, 60, 90 and 120 mg/kg lysozyme as well as antibiotics for 28 days. From day 14 to day 28 of dietary treatment, piglets fed 90 mg/kg lysozyme had greater average daily gain than piglets fed control diet. During the whole experimental period, piglets fed 120 mg/kg lysozyme tended to have greater average daily gain than piglets fed control diet. Compared with piglets fed control diet, piglets fed diets containing antibiotics and 90 mg/kg lysozyme had greater villus height to crypt depth ratio in duodenum and jejunum. Additionally, dietary supplementation of 60 and 90 mg/kg lysozyme as well as antibiotics enhanced the phagocytic activity of peritoneal macrophages in piglets. In conclusion, dietary lysozyme can accelerate the growth of weanling piglets by improving gut health and non‐specific immunity and supplementing 90 mg/kg lysozyme is as effective as antibiotics (20 mg/kg colistin sulphate + 50 mg/kg kitasamycin) in improving the growth performance of weanling piglets.  相似文献   

Effects of apple pomace proportion levels on the fermentation quality of total mixed ration silage and its digestibility,preference and ruminal fermentation in beef cows          下载免费PDF全文

Jiachen Fang  Yang Cao  Masatoshi Matsuzaki  Hiroyuki Suzuki 《Animal Science Journal》2016,87(2):217-223

Four Japanese black beef cows were used in a 4 × 4 Latin square to evaluate the fermentation quality, digestibility, ruminal fermentation and preference of total mixed ration (TMR) silages prepared with differing proportions of apple pomace (AP). Experimental treatments were the control (no AP added, CAP), 5% (low, LAP), 10% (medium, MAP) and 20% (high, HAP) of TMR dry matter (DM) as AP. All TMR silages were well preserved. Ethanol was produced in silages containing AP and the amount increased with the proportion of AP (P < 0.05). Nutrient digestibility with LAP, MAP and HAP treatment was lower than that with CAP treatment (P < 0.05). The ruminal molar proportion of acetic acid increased (P < 0.05), but the ruminal ammonia‐N concentration decreased (P < 0.05) as the proportion of AP increased. The preference of the animals was highest for HAP, followed by MAP, CAP and LAP. This study demonstrates that decrease in nutrient digestibility might be related to the ethanol produced naturally from AP. Therefore, the proportion of AP in TMR silages should be less than 5% of dietary DM.  相似文献   

Effects of apple pomace‐mixed silage on growth performance and meat quality in finishing pigs          下载免费PDF全文

Jiachen Fang  Yang Cao  Masatoshi Matsuzaki  Hiroyuki Suzuki  Hirofumi Kimura 《Animal Science Journal》2016,87(12):1516-1521

We measured the growth performance and meat quality of 10 crossbred (Yorkshire × Duroc × Landrace) neutered male pigs to evaluate the effects of apple pomace‐mixed silage (APMS). The pigs were divided into two groups and were respectively fed the control feed and the AMPS ad libitum during the experiment. No difference was found in the finished body weight, average daily gain, carcass weight, back fat thickness or dressing ratio between the control and the AMPS treatments, but average dairy feed intake (dry matter) was significantly lower and feed efficiency was significantly higher using the APMS treatment (P < 0.05). With regard to meat quality, the APMS increased the moisture content but decreased the water holding capacity (P < 0.05) compared with the control treatment. Furthermore, the APMS affected the fatty acid composition of the back fat by increasing linoleic acid (C18:2n6), linolenic acid (C18:3) and arachidic acid (C20:0) levels, while decreasing palmitic acid (C16:0), palmitoleic acid (C16:1) and heptadecenoic acid (C17:1) levels, compared with the control treatment. These results indicate that feeding fermented apple pomace to finishing pigs increases the feed efficiency and affects the meat quality and fatty acid composition of back fat.  相似文献   

Rearing conditions affected responses of weaned pigs to organic acids showing a positive effect on digestibility,microflora and immunity          下载免费PDF全文

Yalin Zhang  Yumo Song  Xiaoling Zhang  Yan Lin  Lianqiang Che  Shengyu Xu  De. Wu  Bai Xue  Zhengfeng Fang 《Animal Science Journal》2016,87(10):1267-1280

Three experiments were conducted to assess the response of weaned pigs to organic acid SF3, which contains 34% calcium formate, 16% calcium lactate, 7% citric acid and 13% medium chain fatty acids. Dietary treatments had no effect on growth performance of piglets (21‐day weaning) fed the commercial prestart diet for 1 week before receiving the experimental diets supplemented with SF3 at 0, 3 or 5 g/kg diet (Exp. 1), whereas diarrhea frequency averaged across a week was decreased by SF3 supplementation (5 g/kg diet) in piglets fed the experimental diets immediately after weaning (Exp. 2). In Exp. 3, piglets (28‐day weaning) were fed the control (containing pure colistin sulfate and enramycin, respectively, at 20 mg/kg diet) for 1 week and then were fed the control or SF3‐supplemented (5 g/kg diet) diet for 2 weeks. The SF3‐fed piglets had greater apparent ileal digestibility of calcium and dry matter, while also demonstrating greater overall gross energy, up‐regulated jejunal expression of sodium‐glucose cotransporter‐1 and transforming growth factor‐β, down‐regulated jejunal expression of tumor necrosis factor (TNF)‐α, higher ileal Lactobacillus, with lower total bacteria content, lower plasma TNF‐α but higher IgG levels than the control‐fed piglets. Collectively, SF3 consumption improved diarrhea resistance of weaned pigs by improving nutrient digestibility, piglet immunity and intestinal bacteria profile. © 2016 Japanese Society of Animal Science  相似文献   

光质和光强对红树莓组培苗叶绿素荧光参数的影响     

郭芳  王超  刘海鹏  李保国  张雪梅  齐国辉 《北方园艺》2016,(11):19-23

为了确立红树莓组培苗生长的适宜光质和光强,以红树莓"海尔特兹"组培苗为试材,研究了不同光质和光强处理下红树莓生根组培苗的叶绿素含量及叶绿素荧光参数。结果表明:光质处理中,除R/B(红光∶蓝光=1∶1)处理外,Chl a(叶绿素a)、Chl b(叶绿素b)和Ct(叶绿素总含量)均随着R/B比率的增加呈现先增加后减小的趋势,Chl a和Ct均在2R/B(红光∶蓝光=2∶1)处理下呈现最大值,分别为1.344、1.716mg·g~(-1),Chl b则在R/2B(红光∶蓝光=1∶2)处理最高,为0.423mg·g~(-1),Chl a、Chl b和Ct均以10R/B(红光∶蓝光=10∶1)处理最低,分别为0.130、0.033、0.163mg·g~(-1),均显著低于其它光质;Chl a/b(叶绿素a/b)在10R/B、2R/B和R/B处理下较高,分别为3.942、3.709、3.653,呈现阳生植物的特性,而在6R/B(红光∶蓝光=6∶1)、W(白光)、4R/B(红光∶蓝光=4∶1)等处理下较低,分别为2.729、2.730、2.747,呈现阴生植物的特性;在R/2B处理下,红树莓组培苗叶片的叶绿素荧光参数值表现极优,Fv/Fm(PSⅡ最大光化学效率)值达到0.881,而10R/B处理下,Fv/Fm值降低到0.355,且显著低于其它光质处理;光强处理中,随着光强的增加,Chl a、Chl b和Ct呈现递减的趋势,最大值由1.218、0.503、1.720mg·g~(-1)分别降低到0.907、0.302、1.209mg·g~(-1),均形成显著差异,Chl a/b则表现为随着光强的增加而增加,在2 000lx处理达到最大值,为3.042,且3种光强处理差异显著;叶片的Fo(初始荧光)随着光强的增加而增加,在2 000lx处理下达到最大值,为180.000,Fm(最大荧光值)则在1 500lx处理下达到最大值,为907.000,而叶片的Fv/Fm、Fv/Fo(PSⅡ的潜在活性)、ΦPSⅡ(PSⅡ实际光化学效率)、Rfd(可变荧光下降比值)则均随着光强的增加呈现递减的趋势,均在1 000lx处理下呈现最大值,分别为0.847、5.573、0.815、4.200。  相似文献   

不同颜色色板对脐橙果园昆虫的诱集效果     

张伟  徐家生  戴小华  郭青云  李国胜  周芳 《北方园艺》2016,(21):111-115

以脐橙果园内昆虫为调查对象,于2014年12月至2015年11月使用紫色、灰色、粉色、蓝色、青色、绿色、黄色、红色、白色和黑色等10种不同颜色的色板对脐橙果园内的昆虫进行诱集,研究了脐橙果园内昆虫群落的组成以及昆虫对不同颜色的趋向性。结果表明:10种颜色色板在脐橙果园内诱集的昆虫有10目48科54种,其中双翅目昆虫诱集数量最多,达到诱虫总数的94.81%;不同颜色色板对脐橙果园昆虫的诱集效果不同,黄色色板诱集的昆虫数量最多,其次是青色和绿色的色板,即波长450~597nm诱虫效果好;粉虱类昆虫更趋向于黄板,而实蝇类昆虫则趋向于青板和绿板;不同月份色板诱集的昆虫个体数存在明显差异。  相似文献