泥鳅激素浸泡催产试验的初报   总被引：1，自引：1，他引：0  

胡廷尖王雨辰  刘士力周志明徐海圣  陈丰刚李倩 宋竞时 《中国农学通报》2012,28(8):82-86

为了寻找一种简易的泥鳅催产方法。浸泡催产激素有绒毛膜促性腺激素(HCG)、促黄体素释放激素类似物(LHRH-A2)和地欧酮(DOM),以HCG250 IU+LHRH-A22 μg+DOM1 mg/尾为基本剂量(X),食盐作为浸泡助渗剂,食用色拉油为消泡剂。分别进行不同浸泡时间、浓度和盐度的泥鳅催产试验。累计激素浸泡催产种鳅13.987 kg,900尾,获受精卵80.218万粒,其催产率在0%~100%,平均催产率为34.66%;得泥鳅水花苗71.42万尾。孵化率为在45.6%~93.3%之间,孵化率平均值为81.21%。激素浸泡催产率高达80.0%,激素浸泡催产获得泥鳅受精卵的孵化率和注射激素催产获受精卵的孵化率差别不大,说明利用激素浸泡泥鳅催产方便省时,是一种有前途的新方法。浸泡催产激素有绒毛膜促性腺激素（HCG）、促黄体素释放激素类似物（LHRH-A2）和地欧酮（DOM）,按照HCG250IU+LHRH-A22μg+DOM1mg/尾基本剂量（X）;食盐为浸泡助渗剂,食用色拉油为消泡剂。2011年6月15日至20日,笔者分别进行不同浸泡时间、浓度和盐度的泥鳅催产试验。累计激素浸泡催产种鳅13.987kg,900尾,获受精卵84.826万粒,其催产率在 0.00%～80.0%,得泥鳅水花苗71.42万尾。孵化率为在45.6%～93.3%之间,孵化率平均值为81.21%。  相似文献   

洛川苹果虫害的动态监测     

刘长海  党志明 《中国农学通报》2012,28(4):273-277

为了研究洛川苹果虫害的发生规律及其动态监测,以对渭北黄土高原果园有害生物生态调控具有实践指导意义。2001-2010年在洛川苹果园,通过田间试验与室内实验相结合的方法,对苹果虫害进行了动态监测。结果表明：该地区果园虫害不同年度的发生规律和危害评价有不同程度的差异。因此,每年和常年动态监测可为苹果虫害的生态调控提供更准确的信息。  相似文献   

侵染葫芦的黄瓜绿斑驳花叶病毒广西分离物分子鉴定   总被引：1，自引：0，他引：1  

秦碧霞  蔡健和  刘志明  魏源文  谢玲  朱桂宁 《植物保护》2008,34(1):116-118

从广西南宁市郊温室大棚中的葫芦[Lagenaria siceraria(Molina)Stand.]上采集到一个表现脉绿、花叶症状的病毒样品,ELISA检测表明,该样品与黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)有密切的血清学关系,利用RT-PCR方法从样品中扩增获得约500bp的DNA片段,序列分析表明,该片段是CGMMV的外壳蛋白基因,暂将该病毒分离物定名为GX-BG。外壳蛋白基因核苷酸序列系统进化树分析表明,已报道的CGMMV主要分为3大群体,GX-BG与中国辽宁分离物(CGMMV-LN)分别属于不同的群体。  相似文献   

云南蔗区甘蔗地下害虫猖獗原因及防治对策     

李文凤  黄应昆  卢文洁  罗志明 《植物保护》2008,34(2):110-113

在总结云南甘蔗地下害虫发生特点的基础上,分析猖獗发生的原因,主要与复杂性虫源、寄主植物、种植制度、天敌等因素有关。根据地下害虫的发生为害特点,提出以农业防治、生物防治为基础,物理防治灯光诱杀为辅,减少虫源;统一化防为重点和抓好关键时期科学用药的综合防治措施,可持续、有效控制甘蔗地下害虫发生为害。  相似文献   

低温胁迫对苦瓜成苗及幼苗生理生化特性的影响   总被引：2，自引：0，他引：2  

程世强  吴智明  曾晶  林彦燕  胡开林 《热带作物学报》2011,32(11):2099-2103

以苦瓜自交系DTFS和11-13-1-3-4为材料,研究低温胁迫对发芽种子成苗的影响以及低温胁迫下苦瓜幼苗叶片质膜相对透性、超氧化物歧化酶(Supeuoxide dismutase,SOD)活性、过氧化氢酶(Catalase,CAT)活性和过氧化物酶(Peroxidase,POD)活性、游离脯氨酸含量、可溶性糖含量以及根系活力的变化规律。结果表明,低温胁迫显著降低了苦瓜发芽种子的成苗率,低温处理时间越长,发芽种子的成苗率越低;12℃低温持续7~9 d基本不会对苦瓜成苗产生重大伤害。低温胁迫使苦瓜幼苗叶片细胞内质膜的相对透性、SOD和POD活性、游离脯氨酸和可溶性糖含量增加,使苦瓜幼苗叶片CAT活性和幼苗的根系活力降低。  相似文献   

Schizothorax prenanti corticotropin-releasing hormone (CRH): molecular cloning,tissue expression,and the function of feeding regulation     

Tao Wang  Chaowei Zhou  Dengyue Yuan  Fangjun Lin  Hu Chen  Hongwei Wu  Rongbin Wei  Zhiming Xin  Ju Liu  Yundi Gao  Zhiqiong Li 《Fish physiology and biochemistry》2014,40(5):1407-1415

Corticotropin-releasing hormone (CRH) is a potent mediator of endocrine, autonomic, behavioral, and immune responses to stress. For a better understanding of the structure and function of the CRH gene and to study its effect on feeding regulation in cyprinid fish, the cDNA of the CRH gene from the brain of Schizothorax prenanti was cloned and sequenced. The full-length CRH cDNA consisted of 1,046 bp with an open reading frame of 489 bp encoding a protein of 162 amino acids. Real-time quantitative PCR analyses revealed that CRH was widely expressed in central and peripheral tissues. In particular, high expression level of CRH was detected in brain. Furthermore, CRH mRNA expression was examined in different brain regions, especially high in hypothalamus. In addition, there was no significant change in CRH mRNA expression in fed group compared with the fasted group in the S. prenanti hypothalamus during short-term fasting. However, CRH gene expression presented significant decrease in the hypothalamus in fasted group compared with the fed group (P < 0.05) on day 7; thereafter, re-feeding could lead to a significant increase in CRH mRNA expression in fasted group on day 9. The results suggest that the CRH may play a critical role in feeding regulation in S. prenanti.  相似文献   

Effects of IBA on the rooting of branch cuttings of Chinese jujube (<Emphasis Type="Italic">Zizyphus jujuba</Emphasis> Mill.) and changes to nutrients and endogenous hormones     

Fengxia Shao  Sen Wang  Wen Huang  Zhiming Liu 《林业研究》2018,29(6):1557-1567

‘Zhongqiusucui’ jujube secondary shoots were treated with 3-indolebutyric acid (IBA) at three concentrations, 500, 1000 and 1500 mg/L. Results show that IBA could significantly enhance rooting and root characteristics of cuttings and were best with IBA at 1500 mg/L. In the rooting process, the formation of adventitious roots was related to the consumption and accumulation of nutrients (soluble sugars and proteins) and the changes in endogenous hormones in phloem, leaf tips and leaf bases. The rooting of cuttings had a positive correlation with the consumption of soluble sugars during the period of callus formation and with the accumulation of soluble sugars during adventitious root formation and growth. Rooting was positively related to the breakdown of soluble proteins in the phloem when the callus formed, and had a positive correlation with its accumulation during adventitious root formation and growth. Leaf tips and leaf bases showed a reverse trend in changes of soluble protein. However, together with the phloem, leaf tips and leaf bases regulated and controlled the formation and development of adventitious roots. The main activities of soluble proteins exist in the leaf tips as this was the main source of soluble proteins. The relation between rooting and IAA (indole-3-acetic acid) content in phloem was positive and thus a high concentration of IAA could benefit the induction and formation of adventitious roots. However, rooting was negatively related with ABA (abscisic acid) and GA (gibberellic acid) and a high concentration of both could inhibit the induction and formation of adventitious roots. Rooting had a positive correlation with phloem IAA/ABA ratios, and higher ratios could improve rooting. Low concentrations of ZR (zeatin riboside) triggered the induction of adventitious roots, while higher concentrations promoted root growth. Endogenous hormones in leaf tips and bases had an impact on rooting. The activities of endogenous hormones mainly existed in leaf tips because they play a major role in the production and consumption of IAA and its ABA content increased during rooting. The ZR in leaf tips influenced the rooting of cuttings, especially in the callus formation and rooting stage. Leaf tips were the main source of GA.  相似文献   

Evaluation of ultrasonic-assisted dyeing properties of fast-growing poplar wood treated by reactive dye based on grey system theory analysis     

Xiaoqian Wang  Zhiming Yu  Yang Zhang  Chusheng Qi  Wen-lung Chang 《Journal of Wood Science》2018,64(6):861-871

Ultrasonic-assisted dyeing as a novel and eco-friendly method was utilized to improve decorative value of fast-growing tree species. The effects of ultrasonic-assisted dyeing parameters (e.g., temperature, dye concentration, ultrasonic power, dipping time, dyeing assistant and dye-fixing agent) on the properties of wood were investigated, and the parameters were optimized. Moreover, the main factors mentioned above were determined with grey system theory analysis. Analyses of chemical structure, thermostability, crystallinity, and microscopic morphologies were conducted using fourier transform infrared spectroscopy (FTIR), thermogravimetric (TG), X-ray diffraction (XRD), and scanning electron microscope (SEM), respectively. Results showed that dye uptake and K/S were influenced by the parameters in the following order: dye concentration?>?dye-fixing agent?>?dyeing assistant?>?dipping time?>?ultrasonic power?>?temperature. FTIR indicated that an ether bond was formed between the wood component and the reactive dye. TG and XRD demonstrated that the thermal stability and crystallinity of ultrasonic-assisted dyed wood were both improved. SEM revealed that dye molecules diffused into the cell cavity, wood vessel, aperture, and part of the wood microstructure such as pit membrane, which was mechanically damaged after the ultrasonic-assisted dyeing treatment and created new fluid channels for the dye.  相似文献   

茅苍术生长动态及解剖结构研究     

姚珊  张红瑞  李珅  高致明  马彦秋 《河南农业科学》2017,46(2)

为了研究茅苍术生长动态及其解剖结构,对茅苍术1年生播种苗采样分析,测定了不同时期根茎直径、地上部分和地下部分干物质质量和鲜物质质量、根冠比等指标,同时对与药材品质相关的结构进行解剖分析。结果表明:茅苍术根茎直径的生长呈持续增长趋势,5~6月和8月为快速增长阶段。茅苍术根冠比在7月16日达到最大,总干物质质量的生长动态符合S形生长曲线。茅苍术叶片中可溶性糖含量先上升后下降,在5月7日达到最大。油室密度6月初达到最多,然后迅速下降,油室直径呈现持续上升趋势。7月是茅苍术有效成分苍术素积累较快的时期。  相似文献   

油茶早实丰产示范林调查(英文)     

姚吉霞  覃慧琳  邓荫伟  苏芬玲  张志铭  林晴晴 《农业科学与技术》2017,18(1)

[目的]筛选营建油茶早实丰产林的最佳品种与最适栽培密度。[方法]调查设2个示范基地,一是调查栽培品种与生长量、产量的关系,二是调查栽培密度与生长量、产量的关系。[结果]在不同品种中,岑软2号的树高、地径、冠幅积显著高于长林4号、长林27号,且岑软2号的产果量显著高于长林4号、长林27号;从花芽分化分析,长林4号的花芽分化量显著高于长林27号,而岑软2号与长林27号、长林4号均无显著差异;从不同的栽培密度分析,栽培密度为1.5 m×2 m的树高和冠幅积显著高于1.0 m×1.5 m、2.0 m×2.5 m栽培密度的树高和冠幅;而栽培密度为1.5 m×1.0 m的地径显著高于其他两个栽培密度的地径;从产果量分析,栽培密度为1.5 m×2.0 m的产果量显著高于其他的栽培密度。[结论]岑软2号品种,早期植株生长量大,产果量高,营建早实丰产林的栽培密度采用2.0 m×3.0 m;岑软3号品种,早期产果量高,营建早实丰产林的栽培密度采用1.0 m×1.5 m或1.5 m×2.0 m,第4~6年投产后进行移栽。  相似文献