Identification and characterization of auto-anti-idiotypic antibodies specific for antibodies against porcine reproductive and respiratory syndrome virus envelope glycoprotein (GP5)   总被引：5，自引：0，他引：5  

Jiang Z  Zhou EM  Ameri-Mahabadi M  Zimmerman JJ  Platt KB 《Veterinary immunology and immunopathology》2003,92(3-4):125-135

Auto-anti-idiotypic antibodies (Aab-2s) were detected from pigs experimentally infected with porcine reproductive and respiratory syndrome virus (PRRSV). The Aab-2s were specific against the idiotypic antibodies (Ab-1s) to the envelope glycoprotein GP5 of PRRSV and were detected from serum samples collected between 21 and 98 days post-infection (DPI). Serological characterization indicated that the Aab-2s recognized the idiotype located within or near the antigen-combining sites of the anti-GP5 antibodies, which was shared by both mouse MAb anti-GP5 and swine polyclonal antibodies. The fact that the Aab-2 inhibited the anti-GP5 antibodies from binding to PRRSV and that they were detected at different time periods in pigs that cleared the infection prior to 98 DPI versus pigs in which virus was detected at 98 DPI suggests that Aab-2 antibodies may play a role in immunity to PRRSV infection.  相似文献   

Dermal mass aspirate from a Persian cat     

Zimmerman K  Feldman B  Robertson J  Herring ES  Manning T 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2003,32(4):213-217

A 1-year-old spayed female Persian cat with alopecia and weight loss had numerous variably ulcerated dermal nodules. Cytologic examination of an aspirate of one of the nodules revealed pyogranulomatous inflammation along with septate hyphae and basophilic round bodies, 0.5–1.0 μm in diameter, surrounded by a thin clear halo (arthrospores). The cytologic diagnosis was dermatophytic pseudomycetoma. Histologically, there were dermal granulomas containing poorly staining, septate hyphae with bulbous spores embedded within abundant amorphous eosinophilic material (Splendore-Hoeppli reaction), and the histologic diagnosis was pseudomycetoma-associated chronic multifocal severe granulomatous dermatitis with lymphocytic perifolliculitis and furunculosis. Microsporum canis was cultured from the lesion. Pseudomycetomas are distinguished from fungal mycetomas, or eumycotic mycetomas, by the findings of multiple lesions, lack of a history of skin trauma, an association with dermatophytes, most commonly Microsporum canis , and, histologically, lack of true cement material and a more abundant Splendore-Hoeppli reaction in pseudomycetomas. Additionally, pseudomycetomas differ from dermatophytosis, in which lesions are restricted to epidermal structures. Persian cats have a high incidence of pseudomycetoma formation, suggesting a heritable predisposition. The prognosis is fair with systemic antifungal therapy. When examining cytologic specimens from Persian cats with single or multiple dermal nodules, especially if pyogranulomatous inflammation is present, a diagnosis of pseudomycetoma should be suspected and is warranted if arthrospores and refractile septate hyphae are present.  相似文献   

Effect of removal and remixing of lightweight pigs on performance to slaughter weights     

Brumm MC  Ellis M  Johnston LJ  Rozeboom DW  Zimmerman DR;NCR- Committee on Swine Management 《Journal of animal science》2002,80(5):1166-1172

Two experiments were conducted to determine the effect of lightweight pig removal and remixing on performance to slaughter. Experiment 1 was a growing-finishing trial utilizing a total of 900 pigs (26.2+/-0.1 kg initial weight) that were sorted and remixed at a mean replicate BW of 72 kg. Experiment 2 was a wean-to-finish trial (17 d mean wean age; 4.8 kg +/- 0.1 BW) utilizing 225 barrows with sorting and remixing occurring 3 wk after weaning. Treatments were 15 pigs/ pen from initial weight to slaughter (15S), 20 pigs/pen from initial weight to time of sort and remix and then reduced to 15 pigs/pen (20/15), and 15 pigs/pen from time of sort and remix to slaughter comprised of the five lightest pigs from each of three 20/15 pens per replicate (15M). Space allocation was 0.56 m2/pig from 26 to 70 kg and 0.74 m2/pig thereafter in Exp. 1. In Exp. 2, pen size was fixed at 2.44 x 4.27 m. In Exp. 1, there was no effect (P > 0.20) of treatment on performance prior to 70 kg. Least squares means for ADG from time of sort and remix to first pig removal from a pen for slaughter at 113 kg were 0.93, 0.87, and 0.91 kg/d for the 20/15, 15M, and 15S treatments, respectively (P < 0.05). When comparing the population represented by the 20/15 + 15M treatments vs the 15S population, there was no difference (P > 0.20) in ADG, ADFI, feed conversion, or carcass lean content. In Exp. 2, pigs in the 20/15 treatment grew slower (P < 0.05) than 15S pigs for the first 21 d (0.20 vs 0.22 kg/d, respectively) with a lower ADFI (P = 0.06) and no difference in feed conversion. When comparing the population represented by the 20/15 + 15M treatments vs the 15S population after sorting and remixing, there was no effect (P > 0.15) of experimental treatments on ADG, ADFI, feed conversion efficiency, carcass lean content, or daily lean gain. These results suggest that removal of lightweight pigs and remixing of the removed pigs into pens of similar-weight pigs is ineffective in improving the overall performance of a population of pigs during the postweaning period.  相似文献   

Analytical sensitivity of air samplers based on uniform point-source exposure to airborne Porcine reproductive and respiratory syndrome virus and swine influenza virus          下载免费PDF全文

Joseph R. Hermann  Jeffrey J. Zimmerman 《Canadian journal of veterinary research》2008,72(5):440-443

Research and surveillance activities involving airborne pathogens rely on the capture and enumeration of pathogens suspended in aerosols. The objective of this study was to estimate the analytical sensitivity (detection threshold) of each of 4 air samplers for Porcine reproductive and respiratory syndrome virus (PRRSV) and swine influenza virus (SIV). In a 5-min sampling period under controlled conditions, the analytical sensitivity of the AGI-30 (Ace Glass, Vineland, New Jersey, USA), AGI-4 (Ace Glass), SKC BioSampler (SKC, Eighty Four, Pennsylvania, USA), and Midwest Micro-Tek sampler (Midwest Micro-Tek, Brookings, South Dakota, USA) was calculated at 1 × 10^1.1, 1 × 10^1.3, 1 × 10^1.1, and 1 × 10^1.2 median tissue culture infectious dose (TCID₅₀) equivalents for PRRSV and 1 × 10^1.4, 1 × 10^1.1, 1 × 10^1.6, and 1 × 10^1.2 TCID₅₀ equivalents for SIV [per 60 L (5-min sampling period)]. Despite marked differences in sampler design, no statistically significant difference in analytical sensitivity was detected between the samplers for collection of artificially produced aerosols containing cell-culture-propagated PRRSV or SIV.  相似文献   

Aerobic soil metabolism of metsulfuron-methyl     

Yutai Li  W T Zimmerman  M K Gorman  R W Reiser  A J Fogiel  P E Haney 《Pest management science》1999,55(4):434-445

A laboratory study was conducted to determine the degradation rates and identify major metabolites of the herbicide metsulfuron-methyl in sterile and non-sterile aerobic soils in the dark at 20°C. Both [phenyl-U-¹⁴C]- and [triazine-2-¹⁴C]metsulfuron-methyl were used. The soil was treated with [¹⁴C]metsulfuron-methyl (0.1 mg kg⁻¹) and incubated in flow-through systems for one year. The degradation rate constants, DT₅₀, and DT₉₀ were obtained based on the first-order and biphasic models. The DT₅₀ (time required for 50% of applied chemical to degrade) for metsulfuron-methyl, estimated using a biphasic model, was approximately 10 days (9–11 days, 95% confidence limits) in the non-sterile soil and 20 days (12–32 days, 95% confidence limits) in the sterile soil. One-year cumulative carbon dioxide accounted for approximately 48% and 23% of the applied radioactivity in the [phenyl-U-¹⁴C] and [triazine-2-¹⁴C]metsulfuron-methyl systems, respectively. Seven metabolites were identified by HPLC or LC/MS with synthetic standards. The degradation pathways included O-demethylation, cleavage of the sulfonylurea bridge, and triazine ring opening. The triazine ring-opened products were methyl 2-[[[[[[[(acetylamino)carbohyl]amino]carbonyl]amino] carbonyl]-amino]sulfonyl]benzoate in the sterile soil and methyl 2-[[[[[amino[(aminocarbonyl)imino]methyl] amino]carbonyl]amino]sulfonyl]benzoate in the non-sterile soil, indicating that different pathways were operable. © 1999 Society of Chemical Industry  相似文献   

Prediction of Starch Amylose Content Versus Total Grain Amylose Content in Corn by Near-Infrared Transmittance Spectroscopy     

M. R. Campbell  S. R. Mannis  H. A. Port  A. M. Zimmerman  D. V. Glover 《Cereal Chemistry》1999,76(4):552-557

A study was conducted to investigate methods of improving a near-infrared transmittance spectroscopy (NITS) amylose calibration that could serve as a rapid, nondestructive alternative to traditional methods for determining amylose content in corn. Calibrations were developed using a set of genotypes possessing endosperm mutations in single- and double-mutant combinations ranging in starch-amylose content (SAC) from -8.5 to 76%, relative to a standard curve. The influence of three factors were examined including comparing calibrations made against SAC versus grain amylose content (GAC), developing calibrations using partial least squares (PLS) analysis versus artificial neural networking (ANN), and using all samples in the calibrations set versus using progressively narrower ranges of SAC or GAC in the calibration set. Grain samples were divided into calibration and validation sets for PLS analysis while samples used in ANN were assigned to a training set, test set, and validation set. Performance statistics of the validation sets that were considered were the coefficient of determination (R), the standard error of prediction (SEP), and the ratio of the standard deviation of amylose values to the SEP (RPD), which were used to compare all NITS models. The study revealed an NITS prediction model for SAC (R = 0.96, SEP = 5.1%, RDP = 3.8) of similar precision to the best GAC model (R = 0.96, SEP = 2.7%, RPD = 3.5). Narrowing the amylose range of the calibration set generally did not improve performance statistics except for PLS models for SAC in which a decrease in SEP values was observed. In one model, the SEP improved while R and RPD remained constant (R = 0.94, SEP = 4.2%, RPD = 2.8) when samples with SAC values <20% were removed from the calibration set. Although the NITS amylose calibrations in this study are of limited precision, they may be useful when a rough screening method is needed for SAC. For example, NITS may be useful to detect severe contamination during transport and storage of specialty grains or to aid breeders when selecting for amylose content from large numbers of grain samples.  相似文献   

Vegetation diversity in an interconnected ephemeral riparian system of north-central Arizona, USA   总被引：15，自引：0，他引：15  

Julie Crawford Zimmerman  Laura E. DeWald  Peter G. Rowlands 《Biological conservation》1999,90(3):721-228

A vegetation survey was conducted in the Pumphouse Wash canyon system, south of Flagstaff, Arizona. Plant species distribution, abundance, and diversity were quantified within and among canyons in this sensitive ephemeral riparian area. Abiotic variables were examined to see if they could predict vegetation diversity. Results of stand ordination and cluster analysis suggested that variation in vegetation distribution and composition was best explained by a complex temperature/moisture - substrate gradient. Understory diversity in general was related to changes in slope and sand/gravel substrate. Each tributary canyon supported unique plant species and the riparian vegetation in one tributary canyon was significantly less diverse than in each of the other canyons. The two canyons with the lowest understory diversity contained rare plant species not found in the other, more diverse canyons. Thus, overall diversity was demonstrated to be a poor predictor of the presence of rare species. Similarly, abiotic variation was useful at predicting diversity levels but was not useful at predicting species occurrence or ecological quality. In summary, attributes frequently considered of use for predicting conservation value of one type (e.g., diversity) were not good at predicting other conservation values (e.g., rarity).  相似文献   

Chaotic stirring in a tidal system     

Ridderinkhof H  Zimmerman JT 《Science (New York, N.Y.)》1992,258(5085):1107-1111

An analysis of a two-dimensional tidal model of the Wadden Sea reveals Lagrangian chaos in the trajectories of water parcels. The associated chaotic stirring results from the transverse intersection of the stable and unstable curves of hyperbolic fixed points in the Lagrangian residual displacement field (the tidal Poincaré map). This tidal dispersion mechanism produces rapid water exchange along the channel axis and could be representative of many shallow tidal seas.  相似文献   

Porcine toxoplasmosis in Indiana.     

J P Dubey  S E Weisbrode  S P Sharma  N W Al-Khalidi  J L Zimmerman  S M Gaafar 《Journal of the American Veterinary Medical Association》1979,174(6):604-609

Toxoplasmosis was diagnosed at necropsy in a 4-week-old pig from a litter of 16 born to a clinically normal sow. Multifocal acute necrosis of blood vessels and parenchyma was found in the small intestine, mesenteric lymph nodes, liver, lungs, and brain. Numerous tachyzoites, ultrastructurally indistinguishable from Toxoplasma gondii, were found in lesions. The affected pig and seven other littermates died after diarrhea had developed within 1 to 2 weeks of birth. The most likely source of infection was oocysts from cats on the farm.  相似文献   

Few differences found between early- and late-weaned pigs raised in the same environment     

Hohenshell LM  Cunnick JE  Ford SP  Kattesh HG  Zimmerman DR  Wilson ME  Matteri RL  Carroll JA  Lay DC 《Journal of animal science》2000,78(1):38-49

Segregation and medicated early weaning are technologies used to optimize the productivity and health of pigs, but these practices may also cause aberrant behaviors indicative of stress. Thus, differences in early- (=10 d of age) and late- (=30 d of age) weaned pigs were investigated. At weaning, pigs were housed in groups of four in 16 pens (eight pens per treatment) in the same facility, and, thus, they were not segregated. Body weights were recorded at birth, weaning, and at approximately 42, 65, 102, 137, and 165 d of age (at slaughter). One-minute, instantaneous scan samples during a 10-min period (at 0600, 1000, 1400, and 1800) were used to record the frequency of lying, standing, and sitting, total number of drinks, feeder investigations, and time spent playing/fighting on 2, 3, and 4 d after weaning. Five-minute, direct observations of each pig were conducted at approximately 40, 60, 80, and 150 d of age. Direct observations were also made of the entire pen for 10 min at approximately 50, 95, 123, and 160 d of age to record aberrant behaviors. At 62 d of age, a handling and blood collection stress was imposed. At 165 d of age, a second stress test was conducted in response to rough handling and transport. Early-weaned pigs spent more time playing/ fighting (P < .006) than late-weaned pigs during the 4 d after weaning, manipulated conspecifics more often at 40 d of age (P < .002), had greater percentage of hemoglobin (P < .03) during Stress Test 1, had greater ADG at 42 d of age (P < .03), and had greater hypothalamic growth hormone-releasing hormone receptor mRNA at slaughter (P < .06). Late-weaned pigs had greater ADG between 137 and 165 d of age (P < .03) and greater pro-opiomelanocortin at slaughter (P < .04). Overall, most differences found between early-weaned and late-weaned pigs were evident soon after weaning, but they disappeared before slaughter.  相似文献