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Antiserum prepared against a phage which infects a Chlamydia psittaci isolate recovered from domestic ducks was used to screen other recent avian C psittaci isolates by indirect immunofluorescence. Two more phage infected strains from ducks were discovered. However, phage was not detected in every isolate examined from common source ducks, although such birds are likely to be infected with the same C psittaci strain. Moreover, phage could not always be demonstrated by indirect immunofluorescence in McCoy cell monolayers infected with the phage-containing strain. The results suggest that phage infection is probably an integral part of duck chlamydiosis in the United Kingdom at present, but that the infection is often cryptic.  相似文献   
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Protectant fungicides rely for their selectivity on physical barriers (e.g. the leaf cuticle) between their deposits and the protoplasts of the host plant. Systemic fungicides, by contrast, come into direct contact with host cells and need to be more selective. Nevertheless, important side-effects can occur, involving higher plants, animals and other microorganisms; examples of these are cited. Sometimes mycelium and spores of the target fungus show differing susceptibility; also, activity of a fungicide in vitro does not always reflect its effectiveness in vivo. An extreme form of selectivity is the undesirable emergence of insensitive strains from an otherwise sensitive fungal species.  相似文献   
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Crystallization of L-cystine is a critical step in the pathogenesis of cystine kidney stones. Treatments for this disease are somewhat effective but often lead to adverse side effects. Real-time in situ atomic force microscopy (AFM) reveals that L-cystine dimethylester (L-CDME) and L-cystine methylester (L-CME) dramatically reduce the growth velocity of the six symmetry-equivalent {100} steps because of specific binding at the crystal surface, which frustrates the attachment of L-cystine molecules. L-CDME and L-CME produce l-cystine crystals with different habits that reveal distinct binding modes at the crystal surfaces. The AFM observations are mirrored by reduced crystal yield and crystal size in the presence of L-CDME and L-CME, collectively suggesting a new pathway to the prevention of L-cystine stones by rational design of crystal growth inhibitors.  相似文献   
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Forty-five Angus steers (avg initial wt 330 kg) were individually fed for 112 d to assess the value of supplemental Zn and source on performance and carcass quality. Steers had ad libitum access to a control diet (81 ppm Zn) of 33% whole corn, 33% ground milo, 15% cottonseed hulls and 13% cottonseed meal, or this control diet with 360 mg Zn/d added from either zinc methionine or zinc oxide. Steers were slaughtered on d 114, and carcass composition was determined by specific gravity. Average daily gain and feed efficiency were not affected by dietary treatments. Steers fed zinc methionine had a higher (P less than .05) USDA quality grade than those fed the control and zinc oxide diets. Marbling score was higher (P less than .05) for steers fed zinc methionine than for those fed control and zinc oxide treatments (4.4 vs 4.0 and 4.0, respectively, where 3 = slight, 4 = small, 5 = modest). Steers fed zinc methionine tended to have more (P less than .10) external fat (13 mm) than steers fed the control diet (10 mm); steers supplemented with zinc oxide had intermediate amounts of external fat (11 mm). Steers fed zinc methionine had 10.5 and 12.8% more (P less than .05) kidney, pelvic and heart (KPH) fat than steers fed control or zinc oxide diets, respectively. The effects of zinc methionine on carcass quality grade and marbling score may be due to Zn and (or) methionine. Regardless of the mechanism, the difference represents a potential economic benefit to producers.  相似文献   
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Bipedalism is a key human adaptation and a defining feature of the hominin clade. Fossil femora discovered in Kenya and attributed to Orrorin tugenensis, at 6 million years ago, purportedly provide the earliest postcranial evidence of hominin bipedalism, but their functional and phylogenetic affinities are controversial. We show that the O. tugenensis femur differs from those of apes and Homo and most strongly resembles those of Australopithecus and Paranthropus, indicating that O. tugenensis was bipedal but is not more closely related to Homo than to Australopithecus. Femoral morphology indicates that O. tugenensis shared distinctive hip biomechanics with australopiths, suggesting that this complex evolved early in human evolution and persisted for almost 4 million years until modifications of the hip appeared in the late Pliocene in early Homo.  相似文献   
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Developing additional techniques for reducing animal feed contamination by rodents and controlling rodent populations is critical to efforts aimed at reducing the occurrence of Salmonella spp infection on poultry farms. Capsaicin, a compound found in chili peppers of the genus Capsicum, produces a burning sensation in the mouth of mammals and is used effectively as an animal deterrent for some pest species. Applied to poultry feed, capsaicin may be effective as an aversive agent to deter rodent feeding and enhance acceptability of rodenticide baits. We tested capsaicin-treated poultry diets (2000 and 3000 Scoville Heat Units, SHU) in no-choice feeding trials at four active New York farms in the winter of 1997-1998. At all farms, consumption of the 2000 SHU diet by rodents (Norway rats, Rattus norvegicus (Berk), and house mice, Mus musculus L) was significantly less than consumption of a control diet. Consumption of the 3000 SHU diet by rodents was significantly less than consumption of a control diet at three of the four farms. Overall, consumption of treated diets was 58-97% and 55-98% less than consumption of the control diet, for the 2000 and 3000 SHU diets, respectively. These reductions appeared to be related closely to the availability of alternative feed sources at these farms. Two-choice feeding trials involving a rodenticide bait (0.05 g kg(-1) brodifacoum) and the 3000 SHU diet demonstrated that Norway rats preferred the rodenticide to the capsaicin-treated poultry feed. Overall, rodenticide bait acceptance was high (95.6%) when offered simultaneously with capsaicin-treated poultry feed. Although poultry managers must utilize several techniques to manage rodent pests, the use of capsaicin-treated diets to reduce feed losses and increase rodenticide bait acceptance appears promising. Use of capsaicin-treated feed on poultry farms may substantially reduce feed contamination by rodents and ultimately the incidence of Salmonella infection in poultry.  相似文献   
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