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41.
Naturally occurring antimutagenic factors, especially those of plant origin, have recently become a subject of intensive research. Antimutagenic properties of terpenoid fractions of sage (Salvia officinalis) were tested in mammalian system in vivo through examining the ability of sage to decrease the frequency of aberrant cells induced by a potent mutagen. First, groups of mice were treated with three concentrations of sage alone and it was established that the frequency of aberrant cells after treatment with a concentration of 25 microL/kg was not significantly different from the negative control (olive oil), while that found after treatment with the 50 microL/kg concentration differed significantly (chi2(1) = 4.05, p < 0.05). Sage used at a concentration of 100 microL/kg was cytotoxic. Mitomycin C (MMC), known as a potent mutagen, was used for induction of chromosome aberrations. Post-treatment with sage suppressed the effects of MMC significantly. Both concentrations (25 microL/kg and 50 microL/kg) produced a significant decrease in the frequency of aberrations relative to MMC (chi2(1) = 5.42, p < 0.02, chi2(1) = 14.93, p < 0.001, respectively). The percent of aberrations decreased with increasing concentrations of sage. Only nontoxic concentrations of sage without mutagenic effects can be recommended for use as inhibitors of mutagenesis or carcinogenesis. 相似文献
42.
Antimicrobial resistance of Salmonella isolated from finishing swine and the environment of 60 Alberta swine farms 总被引:5,自引:0,他引:5
Rajić A McFall ME Deckert AE Reid-Smith R Manninen K Poppe C Dewey CE McEwen SA 《Veterinary microbiology》2004,104(3-4):189-196
The study objective was to describe and evaluate antimicrobial resistance profiles in Salmonella isolated from Alberta swine finishing farms. Salmonella isolates (n = 322) were obtained from 192 fecal and 84 environmental samples of the 60 Salmonella-positive swine finishing farms. Isolates were classified susceptible, intermediate or resistant based on NCCLS guidelines. More than half of the isolates (53.4%) were susceptible to all of the 18 antimicrobials in the testing panel. No resistance was observed to amikacin, amoxicillin/clavulanic acid, cefoxitin, ceftiofur, ceftriaxone, cephalothin, ciprofloxacin, imipenem or nalidixic acid. Less than 1% of isolates were resistant to apramycin, gentamicin and trimethoprim/sulfamethoxazole. Higher frequencies of resistance were observed for chloramphenicol (4.7%), ampicillin (7.8%), kanamycin (11.8%), sulfamethoxazole (21.1%), streptomycin (25.5%) and tetracycline (38.8%). Eleven Salmonella serovars had isolates with resistance to > or =3 antimicrobials. The most frequently resistant serovar was Salmonella Derby, with 27 (38.0%) isolates resistant to > or =3 antimicrobials, including resistance to five and six antimicrobials. An absence of resistance to cephalosporins and fluoroquniolones and a low proportion of isolates resistant to amikacin, amoxicillin/clavulanic acid, apramycin, gentamicin and trimethoprim/sulfamethoxazole are encouraging findings from public health and animal health perspectives. Frequent resistance observed for ampicillin, kanamycin, sulfamethoxazole, streptomycin and tetracycline, antimicrobials commonly used in veterinary medicine for decades, indicates an urgent need to utilize these antimicrobials more prudently if their benefits are to be preserved. 相似文献
43.
Sobocanec S Sverko V Balog T Sarić A Rusak G Likić S Kusić B Katalinić V Radić S Marotti T 《Journal of agricultural and food chemistry》2006,54(21):8018-8026
Native propolis was defined as propolis powder collected from the continental part of Croatia and prepared according to a patented process that preserves all the propolis natural nutritional and organoleptic qualities. Nine phenolic compounds (out of thirteen tested) in propolis sample were detected by high performance liquid chromatography (HPLC) analysis. Among them chrysin was the most abundant (2478.5 microg/g propolis). Contrary to moderate antioxidant activity of propolis examined in vitro (ferric reduction antioxidant power; FRAP-assay), propolis as a food supplement modulated antioxidant enzymes (AOE) and significantly decreased lipid peroxidation processes (LPO) in plasma, liver, lungs, and brain of mice. The effect was dose- and tissue-dependent. The lower dose (100 mg/kg bw) protected plasma from oxidation, whereas the higher dose (300 mg/kg bw) was pro-oxidative. Hyperoxia (long-term normobaric 100% oxygen) increased LPO in all three organs tested. The highest vulnerability to oxidative stress was observed in lungs where hyperoxia was not associated with augmentation of AOE. Propolis protected lungs from hyperoxia by increased catalase (CAT) activity. This is of special importance for lungs since lungs of adult animals are highly vulnerable to oxidative stress because of their inability to augment AOE activity. Because of its strong antioxidant and scavenging abilities, native propolis might be used as a strong plant-based antioxidant effective not only in physiological conditions but also in cases that require prolonged high concentration of oxygen. 相似文献
44.
Interpreting the dependence of soil respiration on soil temperature and water content in a boreal aspen stand 总被引:10,自引:2,他引:8
David Gaumont-Guay T. Andrew Black Tim J. Griffis Alan G. Barr Rachhpal S. Jassal Zoran Nesic 《Agricultural and Forest Meteorology》2006,140(1-4):220
Continuous half-hourly measurements of soil CO2 efflux made between January and December 2001 in a mature trembling aspen stand located at the southern edge of the boreal forest in Canada were used to investigate the seasonal and diurnal dependence of soil respiration (Rs) on soil temperature (Ts) and water content (θ). Daily mean Rs varied from a minimum of 0.1 μmol m−2 s−1 in February to a maximum of 9.2 μmol m−2 s−1 in mid-July. Daily mean Ts at the 2-cm depth was the primary variable accounting for the temporal variation of Rs and no differences between Arrhenius and Q10 response functions were found to describe the seasonal relationship. Rs at 10 °C (Rs10) and the temperature sensitivity of Rs (Q10Rs) calculated at the seasonal time scale were 3.8 μmol m−2 s−1 and 3.8, respectively. Temperature normalization of daily mean Rs (RsN) revealed that θ in the 0–15 cm soil layer was the secondary variable accounting for the temporal variation of Rs during the growing season. Daily RsN showed two distinctive phases with respect to soil water field capacity in the 0–15 cm layer (θfc, 0.30 m3 m−3): (1) RsN was strongly reduced when θ decreased below θfc, which reflected a reduction in microbial decomposition, and (2) RsN slightly decreased when θ increased above θfc, which reflected a restriction of CO2 or O2 transport in the soil profile.Diurnal variations of half-hourly Rs were usually out of phase with Ts at the 2-cm depth, which resulted in strong diurnal hysteresis between the two variables. Daily nighttime Rs10 and Q10Rs parameters calculated from half-hourly nighttime measurements of Rs and Ts at the 2-cm depth (when there was steady cooling of the soil) varied greatly during the growing season and ranged from 6.8 to 1.6 μmol m−2 s−1 and 5.5 to 1.3, respectively. On average, daily nighttime Rs10 (4.5 μmol m−2 s−1) and Q10Rs (2.8) were higher and lower, respectively, than the values obtained from the seasonal relationship. Seasonal variations of these daily parameters were highly correlated with variations of θ in the 0–15 cm soil layer, with a tendency of low Rs10 and Q10Rs values at low θ. Overall, the use of seasonal Rs10 and Q10Rs parameters led to an overestimation of daily ranges of half-hourly Rs (ΔRs) during drought conditions, which supported findings that the short-term temperature sensitivity of Rs was lower during periods of low θ. The use of daily nighttime Rs10 and Q10Rs parameters greatly helped at simulating ΔRs during these periods but did not improve the estimation of half-hourly Rs throughout the year as it could not account for the diurnal hysteresis effect. 相似文献
45.
Twelve American Staffordshire terriers, gestational day 60, and 10 dogs de Bordeaux, gestational day 57 were examined in respect of the morphology and morphometry of their intraorbital muscles. The location of the retractor bulbi, recti and oblique muscles was described and the length of the muscles, the length and breadth of their tendons as well as the distance of the distal insertions of the muscle tendons from the corneal limbus were measured. Similarly, the shape of the line of distal insertions was investigated. The measurements were taken with an electronic caliper to the nearest 0.1 mm. The distance insertion-corneal limbus was measured by means of the Hifny and Misk method (1982). The following differences between the breeds in the morphometry of the intraorbital muscles and their tendons were found out. The distal insertions of the tendons of the dorsal, ventral and lateral recti muscles are further from the corneal limbus in American Staffordshire terriers than in dogs de Bordeaux. The muscular funiculi of the retractor bulbi muscle are further from the corneal limbus in dogs de Bordeaux, except the dorsolateral funiculus (1.96/1.94 mm). In addition, there are differences in the morphometry of the intraorbital muscles and their tendons. No differences, however, were found in the morphology of the intraorbital muscle tendons (their insertion line) and their location. The study can be applied to clinical sciences (surgery) and veterinary ophthalmology, in particular. 相似文献
46.
Fan S Baets R Petrov A Yu Z Joannopoulos JD Freude W Melloni A Popović M Vanwolleghem M Jalas D Eich M Krause M Renner H Brinkmeyer E Doerr CR 《Science (New York, N.Y.)》2012,335(6064):38; author reply 38
We show that the structure demonstrated by Feng et al. (Reports, 5 August 2011, p. 729) cannot enable optical isolation because it possesses a symmetric scattering matrix. Moreover, one cannot construct an optical isolator by incorporating this structure into any system as long as the system is linear and time-independent and is described by materials with a scalar dielectric function. 相似文献
47.
Sperm quality is often evaluated through computer-assisted semen analysis (CASA) and is an indicator of boar fertility. The aim of this research was to study the relationship between CASA motility parameters and fertility results in pigs. Insemination records and semen parameters from a total of 45,532 ejaculates collected over a 3-yr period were used. The statistical model for analysis of fertility data from these inseminations included factors related to sow productivity. The boar- and semen-related variance (direct boar effect) were corrected for the effects of individual boar, genetic line of the boar, age of the boar, days between ejaculations, number of sperm cells in an ejaculate, number of sperm cells in an insemination dose, and AI station. The remaining variance was analyzed if semen motility parameters had a significant effect. This analysis revealed significant (P < 0.05) effects of progressive motility, velocity curvilinear, and beat cross frequency on farrowing rate (FR). Total motility, velocity average path, velocity straight line, and amplitude of lateral head displacement affected (P < 0.05) total number of piglets born (TNB). Boar- and semen-related parameters explained 5.3% of the variation in FR and 5.9% of the variation in TNB. Motility parameters, measured by CASA, explained 9% of the boar- and semen-related variation in FR and 10% of the boar- and semen-related variation in TNB. Individual boar and genetic line of the boar affected (P < 0.0001) the variation in FR and TNB. No differences (P > 0.05) were observed between effects of AI stations on fertility outcome, underscoring the objectivity of the CASA system used. Motility parameters can be measured with CASA to assess sperm motility in an objective manner. On the basis of the motility pattern, CASA enables one to discriminate between the fertilizing capacity of ejaculates, although this depends on the genetic line of the boar used in AI stations. 相似文献
48.
Hohšteter M Smolec O Gudan Kurilj A Šoštarić-Zuckermann IC Bata I Grabarevi Ž 《The Journal of small animal practice》2012,53(1):63-66
A domestic ferret was submitted for sterilisation because of right testis enlargement. Oestradiol and cortisol concentrations were within normal physiological ranges, but testosterone was below and progesterone above normal. Microscopically, the right testis, with the exception of a small part of the epididymis, was replaced with neoplastic tissue. The tumour was composed of streams and bundles of closely packed spindle to ovoid cells forming whorls around collagen and capillaries, and separated by a collagenous matrix. In some areas, cells were loosely arranged and separated by a pale myxomatous matrix. The left testis showed atrophy. The majority of neoplastic cells expressed vimentin and S-100 protein, while expression of collagen IV was moderate and there was no expression of glial fibrillary acid protein. On the basis of macroscopical and histopathological findings, and supported by immunohistochemical reactivity, the diagnosis of benign peripheral nerve sheath tumour was made. This is the first report of benign peripheral nerve sheath tumour in ferret testis. 相似文献
49.
Belić M Kušec V Svetina A Grizelj J Robić M Vrbanac Z Benić M Turk R 《Research in veterinary science》2012,93(2):918-920
Biochemical markers of bone turnover have been shown to be useful as inexpensive and noninvasive tools for monitoring skeletal health. The reference range for bone markers in dogs has been set by different age groups. However, other sources of biological variations were not fully investigated in dogs. To explore whether sex influences the interpretation of bone marker data we examined serum bone markers in 33 male and 25 female dogs. The bone markers selected for this study were: bone alkaline phosphatase (BALP) and osteocalcin (OC) as indicators of bone formation, and C-terminal telopeptide (CTx) of type I collagen as marker of bone resorption. All concentrations of bone markers were lower, but still within the reference range reported for dogs. We found statistically significant differences of the median OC and CTx serum concentrations between males and females. The results of this study suggest that there are sex differences in biochemical markers of bone turnover in dogs which should be considered in interpretation of bone marker data. 相似文献
50.
Studies of clinical uses of brain natriuretic peptide (BNP) represent one of the most important advances in cardiology since the introduction of echocardiography as a clinical diagnostic procedure. Defining the clinical potential of BNP in canine cardiology has not been completed yet. The aim of this study is to measure BNP concentrations in healthy German Shepherd dogs of different ages as a baseline in resting and when conventional protocol of the dobutamine stress test (DST) is applied to dogs. Concentrations of BNP were measured in blood serum by the radioimmunoassay method. The values of BNP concentrations were compared to cardiac parameters obtained by standard cardiac diagnostic procedures (radiology, electrocardiography and echocardiography). No significant differences in serum BNP concentrations existed in dogs of different ages. A statistically significant increase in BNP concentrations was registered after DST. These changes in BNP concentrations were related to ST/T electrocardiographic changes, and correlated to changes in the left ventricular internal diameter in systole (LVESD). These data suggest that BNP is not increased in aged dogs with normal cardiac systolic function and renal function, and that myocardial ischemia leads to a significant increase in BNP concentrations even in dogs with normal left ventricular function. 相似文献