Study of the genetic variability of porcine circovirus type 2 detected in Serbia and Slovenia     

Toplak I  Lazić S  Lupulović D  Prodanov-Radulović J  Becskei Z  Došen R  Petrović T 《Acta veterinaria Hungarica》2012,60(3):409-420

Recent variants of porcine circovirus type 2 (PCV2) were obtained from tissues of domestic pigs with porcine circovirus associated disease and from randomly selected wild boar samples from Serbia and Slovenia. A 450-base-pair nucleotide sequence was obtained by PCR from the ORF2. The derived nucleotide and amino acid sequences were aligned and compared to the corresponding region of closely related PCV2 sequences determined in previous years and retrieved from the GenBank. The 30 Serbian and 17 Slovenian PCV2 sequences clustered into three previously determined genotypes (PCV2a: 7), (PCV2b: 38) and (PCV2d: 2). Three major variable regions, concerning 29 amino acid position substitutions within the ORF2, were observed, which further supports the segregation of the detected strains into three separate genotypes. This study indicates that PCV2b is the predominant genotype in Serbia and Slovenia and the detected PCV2 strains are closely related to those previously described in Europe and in other parts of the world.  相似文献   

Human as a source of tuberculosis for cattle. First evidence of transmission in Poland     

Krajewska M  Kozińska M  Zwolska Z  Lipiec M  Augustynowicz-Kopeć E  Szulowski K 《Veterinary microbiology》2012,159(1-2):269-271

  相似文献   

Gap disturbances and regeneration patterns in a Bosnian old-growth forest: a multispectral remote sensing and ground-based approach   总被引：1，自引：0，他引：1  

Matteo Garbarino  Enrico Borgogno Mondino  Emanuele Lingua  Thomas A. Nagel  Vojislav Duki?  Zoran Govedar  Renzo Motta 《Annals of Forest Science》2012,69(5):617-625

Objectives

We examined canopy gap structure and regeneration patterns at the landscape scale using a combination of remote sensing and field-based surveys.

Methods

The study was carried out in the forest reserve of Lom, an old-growth Fagus-Abies-Picea forest located within the Dinaric Alps in the north-western part of Bosnia and Herzegovina. A high-resolution (1-m panchromatic and 4-m multispectral) Kompsat-2 satellite image was orthorectified and classified through an unsupervised pixel-based classification using an artificial neural network method.

Results

This approach allowed the identification of 650 canopy gaps, ranging in size from 32 to 1,776?m². Only 20 intermediate to large gaps (>250?m²) were identified, and they were mainly present near the perimeter of the reserve. The origin of these large openings was associated with past human-caused disturbances or topographic conditions. The species composition of regeneration within large, human-caused gaps differed markedly from small gaps and non-gap sites in the core area of the reserve. Shade-intolerant species dominated the seedling and sapling layers in large openings. The landscape approach employed in this study confirmed the hypothesis that small gaps predominate at Lom, especially within the core area of the reserve.  相似文献   

Changes in the content of free sulphydryl groups during postharvest wheat and flour maturation and their influence on technological quality     

Jelena Tomić  Milica PojićAleksandra Torbica  Slađana RakitaDragan Živančev  Elizabet Janić HajnalTamara Dapčević Hadnađev  Miroslav Hadnađev 《Journal of Cereal Science》2013

The main aim of this study was to determine the changes in free sulphydryl content during postharvest wheat and flour maturation. The content of free sulphydryl groups was determined from wet gluten over 50 days of wheat postharvest maturation and over 14 days of flour maturation by varying incubation temperatures (30 and 37 °C) and incubation times (0, 45, 90 and 135 min). The amount of free sulphydryl groups increased during postharvest wheat and flour maturation as well as with the increase in temperature and gluten incubation time. The additional aim of this study was to find the interrelation between the content of free sulphydryl groups and selected parameters of technological quality by means of Principal Component Analysis. During wheat/flour maturation, the strengthening of protein structure was observed manifested by the increase in gluten index and Mixolab protein network weakening (C2), and changes in dough resistance as well. The incubation temperature of 37 °C affected the weakening of protein structure manifested by the decrease in gluten index and resistance, and increase in extensibility. Strong differentiation of examined varieties in terms of selected quality indicators was observed upon pre-defined maturation period, probably due to the expression of their intrinsic properties.  相似文献   

Histochemical Distribution of Digestive Enzymes in the Intestine of the Common Two‐Banded SeaBream,Diplodus vulgaris,Geoffroy St‐Hilaire 1817     

I. Tlak Gajger  S. Nejedli  Z. Kozarić 《Anatomia, histologia, embryologia》2013,42(3):161-167

  相似文献   

Distribution and Genotypic Characterization of Campylobacter jejuni Isolated from Poultry in Split and Dalmatia County,Croatia     

A. Kovačić  I. Listeš  C. Vučica  L. Kozačinski  I. Tripković  K. Šiško‐Kraljević 《Zoonoses and public health》2013,60(4):269-276

Consumption of poultry contaminated with Campylobacter jejuni has been recognized worldwide as the leading cause of campylobacteriosis. Therefore, the aim of our study was to investigate the prevalence and genotype diversity of Campylobacter jejuni in poultry meat intended for consumption in Split and Dalmatia County, which is the second biggest County in Croatia. Furthermore, we also wanted to discover possibly stable clones of C. jejuni appearing in different samples and periods of time, which would indicate their ability to persist in or adapt to poultry. In the period from March 2008 until June 2010, 834 samples of poultry from various sources were examined using a surface swab technique. Isolation of C. jejuni was performed by Preston broth and Karmali agar. Identification of the isolates was carried out using biochemical tests. C. jejuni was found in 84 of 574 chicken samples (14.6%) and in nine of 260 samples of turkey (3.5%). Pulse‐field gel electrophoresis (PFGE) was used to analyse 61 obtained isolates using SmaI and KpnI. Of 22 different macrorestriction profiles (MRP) that were found, five were detected in poultry from both different locations and periods of time. Samples from 11 locations were found to be contaminated with more than two different genotypes of C. jejuni. Interestingly, the same MRP were found both in poultry declared to be of domestic origin and in the poultry imported from abroad. The prevalence of C. jejuni in poultry samples was in accordance with previously reported results. Genotypic analysis indicated that the population of C. jejuni in Split and Dalmatia County was diverse and that multiple strains of C. jejuni could be found in the same poultry samples. Furthermore, the same genotypes were identified from the samples obtained from different locations and periods of time, which could support the theory of a global existence of certain MRP that are able to persist in or adapt to poultry.  相似文献   

Assessment of the retardation of selected herbicides onto Danube sediment based on small column tests     

Maćerak  Anita Leovac  Ivančev-Tumbas  Ivana  Börnick  Hilmar  Ilić  Gordana  Isakovski  Marijana Kragulj  Maletić  Snežana 《Journal of Soils and Sediments》2019,19(2):964-972

Journal of Soils and Sediments - This study utilizes column tests to investigate the retardation of certain herbicides with different hydrophobicities (atrazine, alachlor and trifluralin) during...  相似文献   

Transformation of Petroleum Saturated Hydrocarbons during Soil Bioremediation Experiments     

Branimir Jovančićević  Mališa Antić  Ivona Pavlović  Miroslav Vrvić  Vladimir Beškoski  Alexander Kronimus  Jan Schwarzbauer 《Water, air, and soil pollution》2008,190(1-4):299-307

This paper presents transformations of saturated hydrocarbons of petroleum type pollutants during ex situ bioremediation of soil on the pilot heap (halde), during a period of 6 months, within the grounds of Petroleum Refinery Pan?evo (Serbia). Samples for analysis were taken in time intervals of 2 weeks (P₁–P₁₂ samples). Organic substance was extracted by Soxhlet’s method and quantified. Isoprenoid aliphatics, in particular pristane and phytane, and polycyclic aliphatics of sterane and triterpane types in saturated hydrocarbon fractions were analysed by GC-MS (SIM method). Significant amounts of n-alkanes have not been detected. The MS-chromatogram revealed only marginal amounts of pristane and phytane in sample P₁. Pristane and phytane occurred in sample P₈, and in even higher quantities in the final sample P_12. The proceeding bioremediation process was accompanied by the decrease of the relative amounts of pentacyclic terpanes of hopane type, compared to tri- and tetracyclic terpanes. In the initial sample P₁ the distribution of steranes and hopanes follows a pattern, which is characteristic for crude oils. However, their identification by SIM method was not possible in samples P₈ and P₁₂ because of the reduced concentration. The observed changes in the alkane fractions’ compositions may be considered as atypical, referring to the fact that during oil biodegradation under natural conditions, decomposition of isoprenoids occurs much easier and faster than decomposition of polycyclic alkanes of tri-, tetra- and pentacyclic terpane, sterane and diasterane types, after the decomposition of n-alkanes has been almost completed.  相似文献   

Wind deposition of mud aggregates and their role in development of lamellae in the Fair Oaks Dunes,Indiana     

Zoran Kilibarda  Erin ArgyilanJoe Blockland 《CATENA》2008

Three parabolic dunes from the Fair Oaks Dune field in northern Indiana were excavated, in order to study the properties and genesis of lamellae. Reddish lamellae with sharp upper boundaries and diffuse lower boundaries are intercalated with yellowish sand layers within the upper 3–5 m of each dune. The thicknesses of the lamellae decrease from > 2 cm in the east (Winamac dune) to < 0.3 cm in the west (Shelby dune). In deeper parts of the dunes lamellae were absent, but straight or slightly convex, clay rich depositional laminae were present. Thin sections of lamellae reveal that most of the clays are depositional with a lesser clay amount resulting from in situ weathering of feldspar grains. Clays were deposited as sand-size mud (silt/clay) aggregates that winds removed from glacio-fluvial valleys. Lithic fragments, primarily mud aggregates and carbonates, are much more abundant in unweathered depositional laminae than in weathered lamellae. Weathering and depletion of exchangeable Ca⁺ and Mg⁺ led to breakdown of mud aggregates and clay dispersion from clay rich sand laminae through clay-poor sand laminae. The reddish color of lamellae results from oxidation of mafic grains. Sand-size mud aggregates were lighter than quartz or feldspar grains and were carried further downwind and, as a result, the clay in the dunes, and the thickness of the lamellae, increases to the east. At 5.6 m depth in the Winamac dune we found the straight depositional laminae transitioning into wavy lamellae. This is the most direct evidence of depositional lamellae (petrogenic) formation in the literature to date.  相似文献   

Molecular detection of Theileria sp. in ticks and naturally infected sheep     

Duh D  Punda-Polić V  Trilar T  Avsic-Zupanc T 《Veterinary parasitology》2008,151(2-4):327-331

Seven healthy sheep and 10 sheep diagnosed with piroplasmosis based on clinical signs were tested for the presence of babesiae and theileriae. Using the molecular techniques, two species of theileriae were detected and characterized. Theileria ovis was present mostly in healthy sheep and in Rhipicephalus ticks collected from infected sheep. Theileria sp. OT3 parasite was detected mostly in ill animals which represent additional evidence to the possible pathogenic nature of Theileria sp. OT3. The presence of babesiae in sheep or in ticks was not determined. The results of this study showed that ovine piroplasmosis due to Theileria is present in Southern Croatia. It was concluded that clinical diagnosis of ovine piroplasmosis should be confirmed by molecular analysis in order to identify the species of piroplasm, to select the appropriate treatment and to exclude the threat for public health.  相似文献