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为了研究中药复方药液对猪高致病性蓝耳病的疗效,试验采用蒸馏、水煮醇沉等工艺制备中药药液,使生药含量为1.5 g/mL,并测定其对试验猪的最大无毒浓度;采用预防给药、治疗给药和同时给药三种方式观察中药复方药液对人工感染高致病性蓝耳病病毒试验猪的疗效,并在此基础上对江苏4个县区部分发生高致病性蓝耳病猪场的病猪进行治疗,观察该中药复方药液对临床发病猪的治疗效果。结果表明:该中药复方药液最大无毒浓度为1.5 g/mL。人工感染试验猪以预防性给药效果最好,治愈率为85.71%,淋巴结、肺脏、脾脏出血率均为14.28%,肾脏出血率为0,增重率为51.68%。临床发病猪平均治愈率为84.38%。说明该中药复方药液可用于治疗高致病性蓝耳病,以预防性给药效果较好。 相似文献
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为了探究三种不同干奶方法对干奶期奶牛乳房炎发病率的影响,试验选择江苏省某规模化奶牛场体重(582±41)kg、2~4胎、体况良好,乳房、乳汁均正常,即将进入回乳期,泌乳量为(15±2)kg的妊娠后期中国荷斯坦奶牛60头,随机分成A、B、C 3个组,每组20头,分别采用骤然干奶法、快速干奶法和逐渐干奶法进行干奶。在干奶后密切观察乳房情况,并记录奶牛发生乳房炎的头数。结果表明:A组、B组、C组奶牛乳房炎发病率分别为35%、25%和15%。说明逐渐干奶法最优,比较适合广泛应用。 相似文献
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指纹法研究花岗岩区典型崩岗小流域悬浮泥沙来源 总被引：4，自引：2，他引：2
为研究花岗岩区典型崩岗小流域内悬浮泥沙来源,以安溪县龙门镇崩岗侵蚀小流域为研究对象,在4种土地利用类型(侵蚀林地、茶园、耕地和崩岗侵蚀区)中共采集85个泥沙源地土样,同时在河道布设采样器收集降雨后侵蚀悬浮泥沙。通过分析样品中的34种指纹因子,运用复合指纹法筛选出最佳指纹因子组合,并计算出各泥沙源地的泥沙运移规律。结果表明:不同泥沙源地悬浮泥沙中同种指纹因子存在显著差异,利用Kruskal-Wallis检验和多元判别筛选出Ca、Li、Sn、K和Ba为最佳指纹因子组合(累积贡献率大于90%);同时利用多元混合模型得出茶园的悬浮泥沙相对贡献百分比为33%,耕地为27%,侵蚀林地和崩岗侵蚀区均为20%,且混合模型优度拟合检验值为0.89。进一步分析表明,降雨对崩岗侵蚀区的影响最大,茶园和崩岗侵蚀区单位面积产生悬浮泥沙量明显高于其他两个源地,说明崩岗侵蚀区和茶园是花岗岩区崩岗小流域中需要采取防治措施的重点。 相似文献
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冷冻保存前平衡温度对猪精子结构的影响 总被引：1，自引：0，他引：1
研究冷冻保存前平衡温度对精子结构的影响,为提高猪精子冷冻效果提供参考。新鲜猪精液(Ⅰ组)稀释后于17℃过夜保存(Ⅱ组),然后于冷冻Ⅰ液中4℃平衡1.5h(Ⅲ组),再加入预冷的冷冻Ⅱ液于4℃平衡45min(Ⅳ组)。通过精子形态正常率及活力检测,吖啶橙(AO)染色检测精子DNA完整性,碘化丙啶(PI)染色检测精子头部质膜完整性,低渗肿胀试验(HOS)检测精子尾部质膜完整性,异硫氰荧光素标记的花生凝集素(FITC-PNA)染色检测精子顶体完整性,并用扫描电镜(SEM)观察精子结构,评价冷冻保存前平衡温度对猪精子结构的影响。结果表明,与新鲜精液相比,Ⅱ组精液的精子DNA完整性、质膜完整性、顶体完整性等精子结构检测指标所受影响无统计学意义(P0.05);Ⅲ组和Ⅳ组的精子结构检测指标明显受到影响(P0.05),但Ⅲ组和Ⅳ组间无显著差异(P0.05)。扫描电镜检测结果表明,17℃过夜保存精子未见异常,头、颈、尾结构完整,顶体完整、光滑;Ⅲ组精子头部质膜及头颈结合部有轻微损伤;Ⅳ组精子尾部和头颈结合部有不同程度的断裂。冷冻前从17℃降低至4℃及4℃平衡对猪精子结构有一定程度的损伤。 相似文献
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文章旨在探究日粮添加复合甘草素对肉鸡生长性能和免疫功能的影响。试验将磷酸化甘草多糖和甘草提取物混合制成复合物，为复合甘草素（FGS）。试验选用180只1日龄未免疫健康白羽肉鸡，随机分为4组，每组45只，常规免疫新城疫疫苗。其中，3个试验组分别在基础饲粮中添加低、中、高3种剂量的复合甘草素，下文记为FGSL、FGSM、FGSH组，对照组采用常规日粮饲养。试验过程于第7、14、21、28、35天采样，检测体重、血清抗体效价、血清细胞因子含量、sIgA和IL-17含量的变化。结果表明，体重方面：在D7、D21，FGSM组的体重显著高于对照组（P＜0.05），在D35，FGSL和FGSM组体重高于对照组，但无显著差异（P＞0.05）|血清抗体效价变化方面：在各时间段，试验组效价最高，D7各组差异不明显（P＞0.05），但D21、D28、D35，试验组抗体效价均高于对照组（P＜0.05）|在血清细胞因子含量变化方面：在D14、D21、D28，FGSL、FGSM和FGSH组血清IFN-γ含量显著高于对照组（P＜0.05），在D7、D21，FGSL、FGSM和FGSH组的血清IL-2含量高于对照组，但无显著差异（P＞0.05），在D28、D35，FGSL、FGSM和FGSH组的血清IL-2含量显著高于对照组（P＜0.05）|在十二指肠洗液sIgA和IL-17含量变化方面：在D7、D14、D28和D35，FGSL、FGSM和FGSH组血清sIgA含量显著高于对照组（P＜0.05），在D28、D35，FGSL、FGSM和FGSH组的血清IL-17含量显著高于对照组（P＜0.05）。结论：日粮添加复合甘草素能有效增强肉鸡生长性能和免疫功能。
[关键词]复合甘草素|肉鸡|生长性能|免疫功能 相似文献
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本研究旨在研究高蛋白饲料添加虎杖对雏鹅生长性能、肾功能、血清炎症因子和盲肠菌群结构的影响。试验将70只1日龄扬州雏鹅随机分为高蛋白组和高蛋白+3%虎杖组，每组5个重复，每个重复7只，高蛋白组饲喂粗蛋白质为24%的高蛋白饲料，高蛋白+3%虎杖组饲喂含3%虎杖的高蛋白饲料。试验为期15?d。结果显示：与高蛋白组比较，高蛋白+3%虎杖组平均体重、平均日增重显著升高，料重比显著降低（P＜0.05）|血清尿酸、尿素氮和肌酐水平及黄嘌呤氧化酶活性显著降低（P＜0.05）|血清脂多糖（LPS）、IL-6、TNF-α和IL-1β水平均显著降低（P＜0.05）。与高蛋白组比较，高蛋白+3%虎杖组盲肠菌群OTU数、Chao1指数和Shannon指数升高，在门水平，拟杆菌门和变形菌门相对丰度显著降低（P＜0.05），放线菌门相对丰度升高，在属水平，苏黎世杆菌属、乳酸杆菌属和罗斯氏菌属相对丰度升高，拟杆菌属和梭菌属相对丰度降低（P＜0.05）。综上，高蛋白饲料中添加虎杖可以提高雏鹅生长性能，降低血清尿酸和炎症因子水平，改善肾功能。
[关键词]虎杖|雏鹅|高蛋白饲料|肾功能|炎症因子|盲肠菌群 相似文献
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The aim of this study was to evaluate the effects of MCC950 on growth performance, uric acid metabolism, serum inflammatory factor contents and kidney injury of goslings a fed high protein diet, and explore the potential mechanism of nucleotide binding oligomerization domain-like receptor 3 (NLRP3) / cysteine aspartase-1 (Caspase-1) signaling pathway in gosling gout. A total of 120 Yangzhou goslings of 1-day-old were randomly divided into 3 groups, which were control group, high protein group (HP group) and high protein+ MCC950 group (HP+MCC950 group), with 5 replicates in each group and 8 goslings in each replicate. The control group was fed a normal protein diet with 16.5% crude protein, and the HP group and HP+MCC950 group were fed a high protein diet with 24.0% crude protein. The HP+MCC950 group was intraperitoneally injected with MCC950 every 3 days at a dose of 10 mg / kg BW, and the other two groups were injected with the same amount of normal saline. The experiment lasted for 15 days. The results showed as follows: 1) compared with the control group, the average body weight of goslings at 15 days of age in the HP group was significantly decreased (P<0.05); the serum contents of uric acid (UA), creatinine (Cr), urea nitrogen (UN), C-reactive protein (CRP), interleukin-1β (IL-1β), interleukin 18 (IL-18), interleukin 6 (IL-6), tumor necrosis factor α (TNF-α) and transforming growth factor β1 (TGF-β1) in the HP group were significantly increased (P<0.05); the mRNA relative expression levels of NLRP3, Caspase-1, IL-1β and IL-18 and the protein relative expression levels of NLRP3, Caspase-1 and IL-1β in kidney tissues in the HP group were significantly increased (P<0.05). 2) Compared with the HP group, the average body weight of goslings at 10 and 15 days of age in the HP+MCC950 group was significantly increased (P<0.05); the serum contents of UA, Cr, UN, CRP, IL-1β, IL-18, IL-6, TNF-α and TGF-β1 were significantly decreased (P<0.05), while the mRNA relative expression levels of NLRP3, Caspase-1, IL-1β and IL-18 and the protein relative expression levels of NLRP3, Caspase-1 and IL-1β in kidney tissues in the HP+MCC950 group were significantly decreased (P< 0.05). 3) Histological findings showed that the renal tubules of goslings were dilated, and vacuolar degeneration and necrosis occurred in the HP group, while the structure of renal tubules was relatively normal in the HP+MCC950 group. The urate deposition area in kidney tissues of goslings in the HP group was significantly larger than that in the control group (P<0.05), and which in the HP+MCC950 group was significantly smaller than that in the HP group (P<0.05). Moreover, the urate deposition area in kidney tissues of goslings was significantly positively correlated with the protein relative expression level of NLRP3 (r = 0.681, P<0.05). In conclusion, MCC950 can promote the growth of goslings fed a high protein diet and reduce the serum uric acid content, which may be related to the reduction of inflammatory response and kidney injury through its inhibition of NLRP3 / Caspase-1 signaling pathway. © 2023 Authors. All rights reserved. 相似文献