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81.
82.
A previous study showed that a gastric intramural radiolucent band seen on radiographs and a gastric intramural hypoattenuating layer seen on computed tomography were due to the presence of gastric submucosal fat in a cat. There is no known prevalence and distribution pattern of this gastric submucosal fat in cats. This study was carried out to determine the prevalence and distribution pattern of submucosal fat in cat stomachs. Histologic examination of 41 cat stomachs was performed after scheduled killing of cats, obtained from animal shelters. The results revealed that submucosal fat was present in all stomachs. However, thickness and distribution pattern of the submucosal fat varied.  相似文献   
83.
A Japanese Black cow was evaluated for prolonged post‐partum anestrus and enlargement of the right ovary. Transrectal ultrasonography revealed that the right ovary was markedly enlarged and had a solid appearance, while the left ovary was small and inactive. The presumptive diagnosis was directed towards granulosa‐theca cell tumour (GTCT) which was supported by markedly elevated plasma anti‐Müllerian hormone (AMH; 332.0 ng/ml), oestradiol (E2; 103.3 pg/ml) and immunoreactive inhibin (ir‐INH; 2.1 ng/ml) in comparison with the diagnostic cut‐off points for bovine GTCTs. Since the cow had been infertile and had swelling of the udder, slaughter was chosen. Histopathological examination revealed that the tumour was an ovarian sex cord–stromal tumour (SCST) with a Sertoli cell pattern. These findings suggest that plasma AMH, ir‐INH and E2 could be possible biomarkers for bovine ovarian SCST with a Sertoli cell pattern, whereas this case could not be distinguished from GTCTs based on endocrinological profile.  相似文献   
84.
The current report aimed to characterize plasma anti‐Müllerian hormone (AMH) in bovine male pseudohermaphroditism. The blood AMH concentration in a Japanese Black male pseudohermaphrodite calf was compared with pre‐ and post‐pubertal male and female calves and castrated calves. The concentration in the case was higher than in post‐pubertal males, castrated males, and pre‐ and post‐pubertal female calves (p < .05), but similar to that in pre‐pubertal male calves. After extraction of the testes, the concentration in the case dropped to a certain extent. The extracted testes expressed AMH, as detected by immunohistochemistry. This study is the first to show the characterization of AMH in a male pseudohermaphrodite calf. AMH levels in peripheral blood might be useful to diagnose male pseudohermaphroditism in cattle.  相似文献   
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Beer induced the response of the ionotropic gamma-aminobutyric acid receptors (GABA(A) receptors) expressed in Xenopus oocytes, indicating the presence of gamma-aminobutyric acid (GABA)-like activity. Furthermore, the pentane extract of the beer, hop (Humulus lupulus L.) oil, and myrcenol potentiated the GABA(A) receptor response elicited by GABA. The GABA(A) receptor responses were also potentiated by the addition of aliphatic esters, most of which are reported to be present in beer flavor. Aliphatic esters showed the tendency to decrease in the potentiation of the GABA(A) receptor response with an increase in their carbon chain length. When myrcenol was injected to mice prior to intraperitoneal administration of pentobarbital, the pentobarbital-induced sleeping time of mice increased additionally. Therefore, the beer contained not only GABA-like activity but also the modulator(s) of the GABA(A) receptor response.  相似文献   
88.
BACKGROUND: Evidence supports the involvement of nitric oxide (NO) in a variety of male reproductive processes such as spermatogenesis, spermiogenesis, sperm motion, sperm metabolism and sperm capacitation. However, low concentration of NO is essential in biology and physiology of spermatozoa, but high amounts of NO is toxic and has negative effects on sperm functions. On the other hand, it is established that high amounts of NO have detrimental effects on DNA. The integrity of sperm DNA is an important factor in successful fertility and embryo development. It is hypothesized that supra physiological concentrations of NO in seminal plasma cause sperm DNA damage. The aim of this study was to determine sperm DNA damage by comet assay and its correlation with NO level in seminal plasma of fertile and infertile men. METHODS: Semen samples were collected from 45 patients and 70 healthy donors. The stable metabolites of NO (nitrite and nitrate) in seminal plasma were measured by Griess assay and DNA damage was determined using single cell gel electrophoresis (comet) assay method. RESULTS: The NO concentration in the seminal plasma of infertile males was significantly higher than fertile males (5.74+/-1.01 microM/L vs. 3.88+/-0.53 microM/L). There was a significant positive correlation between the NO concentration and sperm DNA comet value in infertile males (P<0.01, R=0.598). CONCLUSION: These results indicate that the overproduction of NO in genital tract of infertile males has a potential pathogenetic role in the reduction of sperm DNA integrity.  相似文献   
89.
BACKGROUND: Phelebotumus papatsi is considered to be the main vector of zoonotic cutaneous leishmaniasis as well as sand fly fever in Iran. There are several measures for vector control, with emphasis on insecticides. The objective of this study was to determine the baseline susceptibility of this vector to the commonly used insecticides in an endemic focus of the disease in central Iran. P. papatasi collected from the field were used for susceptibility status. Its baseline susceptibility to DDT and pyrethroids was assessed on about 6866 specimens collected from Badrood rural district, Esfahan Province, Iran, during the summer of 2010. The LT50 and LT90 values were measured according to the World Health Organisation (WHO) test using probit analysis and regression lines. RESULTS: Results of tests against female P. papatasi revealed LT50 values to DDT 4%, permethrin 0.75%, deltamethrin 0.1%, cyfluthrin 0.15% and lambdacyhalothrin 0.05% of 1104.97, 182.35, 26.79, 15.42 and 1.48 s respectively. The figures for male P. papatasi were 973.51, 59.5, 4.4, 2.65 and 1.5. CONCLUSION: The results of this study provide a guideline for implementation of different vector control measures. Furthermore, guidelines are needed for monitoring and evaluation of insecticide susceptibility tests against sand flies. Copyright © 2012 Society of Chemical Industry  相似文献   
90.
From 1975 to 2000, the water buffalo population in the Brazilian Amazon increased at nearly 13% per year, making it one of the fastest growing herds in the world. On the floodplains of the Amazon River buffalo are managed in a similar manner to cattle, but often earn superior production figures. This production advantage, however, is tempered by the role buffalo play in conflicts between landowners; buffalo are prone to altering the floodplain environment and interfering with production activities such as fishing and farming. In this research, we show that buffalo are kept on the floodplains 24% longer than cattle throughout the year, and 37% longer than cattle during periods when landowner conflicts are most likely to occur. We also show that buffalo productivity is greater than cattle in this system, which gives an opportunity to design management regimes for buffalo that may increase production costs, but that will lower the environmental and social problems that involve buffalo. Specifically, when the waters begin to rise, buffalo should be removed from the floodplains at the same time as cattle. Although this will not lessen the damage done while the buffalo is on the floodplain, it will place buffalo on the floodplain during the dry season when the erosion potential is at its lowest, and reduce the time that buffalo may interfere with other production activities such as fishing. The additional production costs incurred by early removal will not dissipate the production advantage over cattle. Without specific management that addresses the socio-economic and environmental problems caused by buffalo, the continued high growth rate for the buffalo population on the Amazon floodplains may not be sustainable and conflicts will become commonplace.  相似文献   
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