Natural Besnoitia sp. infection in domestic rabbits from Argentina     

Venturini L  Petruccelli M  Píscopo M  Unzaga JM  Venturini MC  Bacigalupe D  Basso W  Dubey JP 《Veterinary parasitology》2002,107(4):273-278

Besnoitia sp. are apicomplexan coccidian parasites affecting several species of mammals and cold-blooded animals in several countries. Besnoitia sp. tissue cysts were seen in several tissues of five rabbits from a rabbit breeder in La Plata, Argentina. Bradyzoites released from macroscopic tissue cysts were inoculated onto bovine monocytes, and into interferon gamma gene knockout (KO) mice. Besnoitia sp. tachyzoites were seen in the peritoneal exudate of KO mice on day 10 pi and these tachyzoites were infective to other KO mice. Tachyzoites grown in cell culture were infective to gerbils (Meriones unguiculatus). This is the first report of Besnoitia sp. infection in any host in Argentina.  相似文献   

Effects of Gonadotrophin Releasing Hormone Administration on the Pituitary-Ovarian Axis in Anoestrous vs Ovariectomized Bitches     

JJCWM Buijtels  NJ Beijerink  HS Kooistra  SJ Dieleman  AC Okkens 《Reproduction in domestic animals》2006,41(6):555-561

The aim of this study was to determine the effects of gonadotrophin releasing hormone (GnRH) administration on the plasma concentrations of reproductive hormones in intact and ovariectomized (OVX) bitches. Therefore, blood samples were collected at multiple times before and after the administration of 10 microg/kg GnRH (Fertagyl)) for the determination of the plasma concentrations of luteinizing hormone (LH), oestradiol, progesterone and testosterone in six anoestrus and in six OVX bitches. The mean plasma LH concentrations before and 60 min after GnRH administration were significantly lower in the anoestrous bitches than in the OVX bitches. In both groups GnRH administration resulted in a significant increase in the plasma LH concentration. The highest plasma LH concentrations were found at 10 min after GnRH administration and these values did not differ significantly between the two groups. Only in the anoestrous bitches a significant increase in plasma oestradiol concentrations was found after GnRH administration and these values were significantly higher than those in the OVX bitches. The plasma concentrations of progesterone and testosterone were low (close to or below the limit of quantitation) both before and after GnRH administration and the differences between anoestrous and OVX bitches were not significant. It can be concluded that (i) basal plasma LH concentration is significantly higher in OVX bitches than in anoestrous bitches, (ii) plasma LH concentration increases after GnRH administration in both anoestrous and OVX bitches, (iii) GnRH administration causes a significant rise in plasma oestradiol concentration only if ovarian tissue is present and (iv) measurement of plasma progesterone and testosterone concentrations before and after GnRH administration does not aid in distinguishing between anoestrous and OVX bitches. The results of this study may provide a basis for the diagnosis of remnant ovarian tissue and verification of neuter status in the bitch.  相似文献   

Pregnancy Rates to Fixed Embryo Transfer of Vitrified IVP Bos indicus,Bos taurus or Bos indicus × Bos taurus Embryos          下载免费PDF全文

LSR Marinho  BV Sanches  CO Rosa  JH Tannura  AG Rigo  AC Basso  JHF Pontes  MM Seneda 《Reproduction in domestic animals》2015,50(5):807-811

The pregnancy rates obtained after the transfer of cryopreserved in vitro‐produced (IVP) embryos are usually low and/or inconsistent. The objective of this study was to evaluate the pregnancy rates of Holstein, Gyr and Holstein × Gyr cattle after the transfer of vitrified IVP embryos produced with X‐sorted sperm. Seventy‐two Gyr and 703 Holstein females were subjected to ovum pickup (OPU) sessions, followed by in vitro embryo production using semen from sires of the same breeds. Embryos (1636 Holstein, 241 Gyr and 1515 Holstein × Gyr) were exposed to forskolin for 48 h prior to vitrification. The pregnancy rate achieved with Gyr dam and sire was 46.1%, which was similar (p = 0.11) to that of Holstein dam and Gyr sire (40.3%). Crossing Gyr dams with Holstein sires resulted in a pregnancy rate of 38.9% and did not differ (p = 0.58) from the pregnancy rate obtained with the cross between Holstein dams and Gyr sires. The rate obtained with Holstein dam and sire was 32.5%. The average pregnancy rate was 36.6%, and no difference was found in the proportion of female foetuses (88.8%, in average) among breeds (p > 0.05). In conclusion, transfer of cryopreserved X‐sorted embryos represents an interesting choice for dairy cattle. Despite the small differences between pregnancy rates, we highlight the efficiency of this strategy for all of the racial groups studied.  相似文献   

Effects of FGF10 on Bovine Oocyte Meiosis Progression,Apoptosis, Embryo Development and Relative Abundance of Developmentally Important Genes In Vitro          下载免费PDF全文

RF Pomini Pinto  PK Fontes  B Loureiro  AC Sousa Castilho  J Sousa Ticianelli  E Montanari Razza  RA Satrapa  J Buratini  C Moraes Barros 《Reproduction in domestic animals》2015,50(1):84-90

Fibroblast growth factor (FGF10) acts at the cumulus oocyte complex, increasing the expression of cumulus cell expansion‐related genes and oocyte competency genes. We tested the hypothesis that addition of FGF10 to the maturation medium improves oocyte maturation, decreases the percentage of apoptotic oocytes and increases development to the blastocyst stage while increasing the relative abundance of developmentally important genes (COX2, CDX2 and PLAC8). In all experiments, oocytes were matured for 22 h in TCM‐199 supplemented with 0, 2.5, 10 or 50 ng/ml FGF10. In Experiment 1, after maturation, oocytes were stained with Hoechst to evaluate meiosis progression (metaphase I, intermediary phases and extrusion of the first polar body) and submitted to the TUNEL assay to evaluate apoptosis. In Experiment 2, oocytes were fertilized and cultured to the blastocyst stage. Blastocysts were frozen for analysis of COX2, CDX2 and PLAC8 relative abundance. In Experiment 1, 2.5 ng/ml FGF10 increased (p < 0.05) the percentage of oocytes with extrusion of the first polar body (35%) compared to 0, 10 and 50 ng/ml FGF10 (21, 14 and 12%, respectively) and FGF10 decreased the percentage of oocytes that were TUNEL positive in all doses studied. In Experiment 2, there was no difference in the percentage of oocytes becoming blastocysts between treatments and control. Real‐time RT‐PCR showed a tendency of 50 ng/ml FGF10 to increase the relative abundance of COX2 and PLAC8 and of 10 ng/ml FGF10 to increase CDX2. In conclusion, the addition of FGF10 to the oocyte maturation medium improves oocyte maturation in vitro, decreases the percentage of apoptotic oocytes and tends to increase the relative abundance of developmentally important genes.  相似文献   

Fibrous ingluvial foreign bodies in 33 cockatiels (Nymphicus hollandicus)          下载免费PDF全文

AC Rosenwax  ML Cowan 《Australian veterinary journal》2015,93(10):381-384

The presentation, treatment and outcomes of 33 ingluvial fibrous foreign bodies in cockatiels (Nymphicus hollandicus) are described. Vomiting, lethargy and weight loss were the most common presenting signs. Diagnosis was made on palpation of a mass in the crop (ingluvies). Both surgical and non‐surgical treatment regimens were evaluated. The overall mortality rate was 33%, suggesting fibrous materials are not safe for cockatiels to groom or ingest and should not be offered as cage accessories.  相似文献   

Physiological and pharmacokinetic responses in neotropical Piaractus mesopotamicus to the essential oil from Lippia sidoides (Verbenaceae) as an anesthetic     

Ventura  Arlene Sobrinho  de Castro Silva  Tarcila Sousa  Zanon  Ricardo Basso  Inoue  Luis Antonio Kioshi Aoki  Cardoso  Claudia Andrea Lima 《International Aquatic Research》2019,11(1):1-12

International Aquatic Research - The goal of the study was to evaluate Lippia sidoides essential oil as an anesthetic for the tropical fish pacu Piaractus mesopotamicus. The physiological and...  相似文献   

Steaming effects on selected wood properties of Turkey oak by spectral analysis     

Luigi Todaro  Luigi Zuccaro  Mario Marra  Bruno Basso  Antonio Scopa 《Wood Science and Technology》2012,46(1-3):89-100

Turkey oak (Quercus cerris L.) is characterized by some technological and aesthetical factors limiting its market value from its great potential. In this study, the effect of direct and indirect steaming on reduction in equilibrium moisture content (EMC) and colour variations was evaluated using a hyperspectral radiometer. Steaming treatments were carried out at 80°C for 48?h, and 120°C for 18 and 24?h, showing a reduction in EMC in the order of 8.1, 28.5 and 13.5, respectively, as well as very significant lightness (L^*) and hue (h°) modifications in comparison with untreated specimens. The spectral signature analysis confirmed that hydrothermal treatments modify wood sensibility to the light source in the entire spectrum range. The study supports the validity of hydrothermal treatments for improving technological and aesthetical properties of Turkey oak.  相似文献   

Toxoplasma gondii infection in sentinel and free-range chickens from Argentina     

Moré G  Maksimov P  Pardini L  Herrmann DC  Bacigalupe D  Maksimov A  Basso W  Conraths FJ  Schares G  Venturini MC 《Veterinary parasitology》2012,184(2-4):116-121

This study aimed at isolating and genotyping Toxoplasma gondii from serologically positive free-range chickens from Argentina, and to evaluate the use of sentinel animals during a short time period of exposure to determine environmental contamination with T. gondii oocysts. Two groups of chickens on six farms were compared in this study: (i) young, 2-3 month-old broiler-type chickens reared as sentinel animals on the farms and (ii) adult chickens reared on the same farms for more than one year. Seroconversion rates of 7.0% or 5.7% were observed in sentinel broiler chickens reared for a period of 74 days (January-April 2010) or 88 days (August-November 2010) respectively, as shown by a T. gondii specific immunofluorescent antibody test. Fifty-three percent (17 of 32) of adult chickens were positive and showed higher titres than sentinel animals. Isolation of T. gondii from tissues (brain and heart) of serologically positive chickens was achieved from six of seven free-range adult birds with IFAT titres of 200 and higher. The isolated parasites were analysed by multi-locus polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The isolated T. gondii showed three different genotypes: two genotypes consisted in atypical allele combinations, and the remaining genotype had exclusively clonal type II alleles. All isolates obtained at a single farm, corresponded to the same genotype. The T. gondii genotypes observed are identical to those described in cats, dogs, chickens and capybaras elsewhere in South America. Two isolates, which showed different allele combinations in PCR-RFLP, were characterized in a mouse virulence assay. While one isolate showed a low virulence a second isolate was of intermediate virulence to mice.  相似文献   

Dietary Levamisole as Immunostimulant for Striped Surubim,Pseudoplatystoma reticulatum     

Ricardo Basso Zanon  Brunno da Silva Cerozi  Jos Eurico Possebon Cyrino  Tarcila Souza de Castro Silva 《Journal of the World Aquaculture Society》2014,45(6):672-680

Immunostimulants are better suited for disease control than using drugs and chemicals as remedies in animal production. This study evaluates effects of dietary levamisole on growth and immunological parameters response of Pseudoplatystoma reticulatum. A trial was set up in a completely randomized design with six treatments – 0.0, 50, 100, 200, 300, and 500 mg levamisole per kg of feed (n = 3) – fed until apparent satiation, twice a day, for 60 d to striped surubim (77.68 ± 2.5 g) randomly stocked in 18 plastic tanks (300 L; 10 fish per tank). Dietary levamisole affected lysozyme activity and concentration, the best effects were recorded for fish fed 247 mg levamisole per kg of feed, that is, levamisole positively influenced innate immune system of striped surubim. However, dietary levamisole did not affect serum protein, albumin and globulins, as well as leukocyte respiratory burst activity and leukocyte proliferation. No differences in growth parameters were recorded. Dietary levamisole improved immunological responses of striped surubim.  相似文献   

Effects of clinical mastitis and puerperal diseases on reproductive efficiency of dairy cows     

Campos  Carla Cristian  do Prado  Francisco Luiz  dos Reis  João Paulo Justo  Carneiro  Luisa Cunha  Silva  Paula Regina Basso  de Moraes  Giovanna Faria  dos Santos  Ricarda Maria 《Tropical animal health and production》2020,52(6):3061-3068

Tropical Animal Health and Production - The objective of this study was to evaluate the effects of clinical mastitis (CM) occurring before or after the first AI postpartum, and puerperal diseases...  相似文献