Assessment of Progesterone Concentration Using Enzymeimmunoassay, for Early Pregnancy Diagnosis in Sheep and Goats     

CM Boscos  FC Samartzi  AG Lymberopoulos  A Stefanakis  S Belibasaki 《Reproduction in domestic animals》2003,38(3):170-174

The objective of this study was to determine a value of serum progesterone (P₄) concentration, assessed using an enzymeimmunoassay (EIA), for the early distinction between pregnant and non‐pregnant ewes and goats. Adult, non‐lactating ewes of Chios (n=53), Berrichon (n=30) and Sfakia (n=45) breeds were synchronized during the breeding season with progestagens and gonadotrophins and mated to fertile rams (Experiment I). Adult, lactating goats of Swiss breeds (Alpine and Saanen, n=104) and indigenous Greek breed (n=45) were synchronized during the transitional season with progestagens, PGF₂α and gonadotrophins. Cervical artificial insemination (AI) with fresh semen was applied once, 42–44 h after sponge removal (Experiment II). Jugular blood samples were collected on day 19 after sponge removal (ewes) or on day 21 after AI (goats) and serum P₄ concentration was determined by EIA. Progesterone concentrations ≥1.0, ≥1.5, ≥2.5 and ≥4.0 ng/ml were tested as indicative of pregnancy. Pregnancy diagnosis was verified on birth. In the case of sheep, using a discriminatory level of 2.5 ng/ml, overall accuracy of pregnancy diagnosis was 91.4% and predictive value of negative and positive diagnoses were 98.3 and 85.3%, respectively. In the case of goats, predictive value of negative diagnosis was 95.8 and 94.0% and predictive value of positive diagnosis 71.3 and 71.7%, for 1.5 and 2.5 ng/ml, respectively; overall accuracy was 79.2% using either level. The other discriminatory levels tested did not improve these results. A significant positive correlation was observed between P₄ concentration and the number of lambs or kids born, and further analysis indicated that this relationship is not a simple linear function. Based on the results of this study, P₄ concentrations of 2.5 ng/ml in the case of ewes and 1.5–2.5 ng/ml in the case of goats, determined with EIA, are proposed as discriminatory levels between pregnant and non‐pregnant animals, at an interval of one oestrous cycle after service.  相似文献   

Evaluation of Toenail Blood Samples for Blood Gas Analysis in the Dog     

JANE E. QUANDT DVM    MARC R. RAFFE DVM  MS  Dipiomate ACVA  ACVECC    DAVID POLZIN DVM  PhD  Dipiomate ACVIM    ELAINE P. ROBINSON BVetMed  MVSc  Dipiomate ACVA  RONALD E. MANDSAGER DVM 《Veterinary surgery : VS》1991,20(5):357-361

Blood gas values were compared in blood collected from cut toenails and femoral arteries in 50 healthy crossbred dogs that were sedated and allowed to breathe room air spontaneously. Blood samples from cut toenails were collected by microcapillary technique with Natelson tubes. Femoral artery samples were collected by arterial puncture. Blood values for PO2, PCO2, pH, and HCO3 were compared. There was good correlation for pH, PCO2, and bicarbonate, but not for PO2. Microcapillary samples should be collected in 10 seconds or less for the most accurate results. A metal mixing "flea" was unnecessary. When properly handled, the Natelson tube technique provides an alternative method for collection of blood gas samples.  相似文献   

Disseminated Mortierella wolfii infection in a neonatal calf     

JS Munday  AG Wolfe  KE Lawrence  SK Pandey 《New Zealand veterinary journal》2013,61(1):62-63

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Butorphanol Does Not Reduce the Minimum Alveolar Concentration of Halothane in Dogs     

JANE E. QUANDT DVM    MARC R. RAFFE DVM. MS  Diplomate ACVA  ACVECC  ELAINE P. ROBINSON BVetMed  MVSc  Diplomate ACVA 《Veterinary surgery : VS》1994,23(2):156-159

This study evaluated the effect of butorphanol tartrate, a synthetic opioid agonist-antagonist, on halothane minimum alveolar concentration (MAC) in dogs. Baseline halothane MAC was determined in each of six dogs. Butorphanol was administered and halothane MAC was redeter-mined. Each dog received butorphanol at 0.2, 0.4, and 0.8 mg/kg intravenously at 1 week intervals. Heart rate and arterial blood pressure decreased after butorphanol administration, but returned to baseline by 50 minutes. There was little effect on respiratory parameters. A halothane-sparing effect was not noted with any butorphanol dose.  相似文献   

Evaluation of 25%, 50%, and 67% Nitrous Oxide with Halothane-Oxygen for General Anesthesia in Horses     

MARCO TESTA DVM  MS    MARC R. RAFFE DVM  MS  DiplomateACVA  ELAINE P. ROBINSON BVetMed  MVSc  DiplomateACVA 《Veterinary surgery : VS》1990,19(4):308-312

Twenty-five percent, 50%, and 67% nitrous oxide was administered to 12 horses anesthetized with halothane and oxygen. Compared to halothane-oxygen alone, there was no significant difference in heart rate, systolic, diastolic, or mean blood pressure values, arterial pH, PaCO2, or plasma bicarbonate values when nitrous oxide was included. A significant linear reduction in PaO2 values could be correlated with N2O:O2 concentrations. The halothane level required to maintain surgical anesthesia was reduced when nitrous oxide was administered, but it was not affected by changing the nitrous oxide concentrations. Nitrous oxide concentrations greater than 25% provide no additional reduction in halothane requirement and may be accompanied by PaO2 values that pose risk to the horse.  相似文献   

A Prosthetic Implant for the Equine Carpal Joint: Surgical Technique and Results     

BRIAN S. GROSSMAN D.V.M.  M.S.  BARRIE D. GRANT D.V.M.  M.S.  RONALD D. SANDE D.V.M.  PH.D.  †  MARC H. RATZLAFF D.V.M.  PH.D.  ‡  ANTHONY M. GALLINA D.V.M.  PH.D.  §  GEORGE BAGBY M.D.  M.S.  | STEVE PYRITZ B.S.M.E.H  ¶ 《Veterinary surgery : VS》1980,9(3):93-102

A prosthesis designed to partially replace the articular surfaces of the radial and third carpal bones was implanted bilaterally in 6 normal horses. The prosthesis consisted of a convex metal alloy component, and a concave ultra-high molecular weight polyethylene component, for implantation into the radial and third carpal bones, respectively. One horse underwent the soft tissue approach only to serve as a control. Effects on joint motion and pathologic changes were monitored over a 6-month period using clinical examination, synovial fluid analysis, electrogoniometry, high-speed cinematography, radiography, and gross and microscopic pathologic examination. Six months following implantation, all horses that received implants would trot, with slight to moderate lameness. Electrogoniometric and cinematographic evaluation of joint motion demonstrated a reduction in amplitude of the carpus in 4 horses receiving implants; however, in 2 horses, the reduction was less than, or equivalent to, that of the control horse. One horse that received the prosthesis was rideable, and was able to run with minimal lameness. Radiography, synovial fluid analysis, and gross and microscopic pathologic examination revealed varying degrees of synovitis and osteoarthritis. Biomechanical alterations of the middle carpal joint and release of wear particles from the prosthesis were considered to be major causes of these secondary changes. Changes in design of the prosthesis, intended to more accurately maintain normal joint biomechanics are proposed.  相似文献   

FAT‐SUPPRESSED SPOILED GRADIENT‐RECALLED IMAGING OF EQUINE METACARPOPHALANGEAL ARTICULAR CARTILAGE     

JULIEN OLIVE  MARC‐ANDRÉ D'ANJOU  CHRISTIANE GIRARD  SHEILA LAVERTY  CHRISTINE THEORET 《Veterinary radiology & ultrasound》2010,51(2):107-115

The purpose was to evaluate the capacity of 1.5 T magnetic resonance (MR) imaging to assess articular cartilage in racehorses with naturally occurring metacarpophalangeal joint osteoarthritis. A sagittal, three‐dimensional spoiled gradient‐recalled echo (SPGR) with fat saturation (FS) sequence was acquired ex vivo on 20 joints. Following joint dissection, specific areas on the third metacarpal condyle were designated for subsequent sampling for histologic cartilage thickness measurement and modified Mankin scoring. Cartilage thickness was measured and cartilage signal intensity was also graded (0–3) on MR images at these selected metacarpal sites. Cartilage structure was graded (0–3) macroscopically and on MR images by two examiners in defined subregions of the proximal phalanx, third metacarpal, and proximal sesamoid bones. There was good precision (mean error 0.11 mm) and moderate correlation (r=0.44; P<0.0001) of cartilage thickness measurements between MR images (0.90±0.17 mm) and histology (0.79±0.16 mm). There was moderate correlation between modified Mankin histologic score and signal intensity of cartilage (r=0.36; P<0.01) or MR cartilage structure assessment (r=0.49, P>0.001) on SPGR‐FS. The sensitivity to detect full‐thickness cartilage erosion on MR was only moderate (0.56), and these lesions were often underestimated, particularly when linear in nature. However, the specificity to detect such lesions on MR was high (0.92). While few limitations were identified, the use of a clinically applicable SPGR‐FS sequence allows a reasonably accurate method to assess structural changes affecting the articular cartilage of the equine metacarpophalangeal joint.  相似文献   

Effect of Ram Age on Structural and Functional Competence of Frozen–Thawed Spermatozoa in Dairy Sheep     

AG Lymberopoulos  IA Tsakmakidis  TAA Khalifa 《Reproduction in domestic animals》2010,45(4):572-578

The objectives of this study were to investigate the influence of ram age on structural and functional competence of frozen–thawed spermatozoa and to test the hypothesis that increasing number of sperm bound to the zona pellucida in vitro was associated with decreasing in vivo fertility of frozen semen. Rams were allocated into two groups. Each group consisted of five rams aged either 1–2 years (young) or 4–5 years (mature). Three successive ejaculates were collected from each ram using an artificial vagina. Only ejaculates of ≥ 2.5 × 10⁹ sperm/ml and 80% sperm progressive motility were pooled per ram, diluted with Bioxcell^® medium and frozen in 0.25 ml straws. The end points of post‐thawing semen evaluation were computer‐assisted cell motility analysis, sperm capacitation (chlortetracycline assay), simultaneous assessment of plasma membrane integrity, mitochondrial membrane potential and condensation status of nucleus, per‐cell analysis of lipid peroxidation using C11‐BODIPY^581/591, sperm‐hemizona binding (HZB) ability and sperm fertility after laparoscopic insemination of ewes (n = 114) in the progestagen‐synchronized oestrus. The results showed that mature rams had significantly lower values of sperm hyperactivated motility and peroxidized sperm, higher percentages of live non‐capacitated sperm and sperm cells with intact plasma membrane, functional mitochondria and condensed chromatin, as well as, greater lambing rate and ewe prolificacy. Sperm HZB binding ability was higher (p < 0.05) for young than for mature rams. Significant correlations were found between number of spermatozoa bound to the zona pellucida and semen fertility (r = ?0.63 to ?0.71). In conclusion, mature rams have better semen quality and in vivo fertility than young rams. Cryocapacitation can be involved in decreasing ram semen fertility as evidenced by the high number of spermatozoa bound to the zona pellucida in vitro.  相似文献   

Heterotopic Transfer of Fresh and Cryopreserved Autogenous Articular Cartilage in the Horse     

MARC R. DESJARDINS DMV  DVSC    MARK B. HURTIG DVM  MVSC  Dipiomate ACVS  NIGEL C. PALMER DVM  PhD 《Veterinary surgery : VS》1991,20(6):434-445

Two 10 mm thick osteochondral grafts were harvested from the lateral aspect of the lateral trochlear ridge of the left talus in each of 10 anesthetized horses. The grafts were frozen in a 7.5% DMSO solution and stored in liquid nitrogen. The horses were anesthetized again on day 14 and the thawed grafts were press-fitted into drill holes in the trochlear ridges of the right stifle. A fresh graft was transferred from the right hock to the left stifle. To control for the effects of surgery, another fresh graft was transferred from the right stifle to the left stifle. The result was two grafts in each femoropatellar joint. Fresh and frozen osteoarticular autografts appeared to maintain a durable weight-bearing surface for 3 months; however, the fresh grafts were clearly superior. Frozen grafts had fewer living chondrocytes, decreased safranin-O staining, and decreased SO435 uptake. Graft stability and articular surface congruency were determining factors in the outcome of all grafts. Since the availability of osteochondral autografts is limited, further work on the use of preserved allogeneic osteochondral tissue is warranted.  相似文献   

Rhodococcus equi infection in goats   总被引：1，自引：0，他引：1  

MJ CARRIGAN  IJ LINKS  AG MORTON 《Australian veterinary journal》1988,65(10):331-332

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