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61.
Frederic Aparicio Salvador Soler José Aramburu Luis Galipienso Fernando Nuez Vicente Pallás Carmelo López 《European journal of plant pathology / European Foundation for Plant Pathology》2009,123(1):117-123
A polyprobe for the simultaneous detection by non-isotopic molecular hybridisation has been developed to detect any of the
following six viruses causing important economic losses in tomato crops: Tomato spotted wilt virus, Tomato mosaic virus, Pepino mosaic virus, Cucumber mosaic virus, Potato Y virus and Parietaria mottle virus. The polyprobe detected all six viruses with similar sensitivity to that obtained using individual riboprobes. In addition,
we evaluated the possible use of the tissue-printing as a sample preparation technique applied to routine diagnosis of tomato
plants with the polyprobe.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
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Encapsulated Stuphylococcus aureus from bovine mastitis 总被引：1，自引：0，他引：1
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Acquired immunity in schistosomiasis with purified Fasciola hepatica cross-reactive antigens 总被引：1，自引：0，他引：1
G V Hillyer M Soler de Galanes M I García Rosa F Montealegre 《Veterinary parasitology》1988,29(2-3):265-280
We have previously demonstrated that a Fasciola hepatica-derived adult worm antigen, which is cross-reactive with Schistosoma mansoni and designated FhSmIII(M), induces resistance to challenge infection with S. mansoni in mice. The current review concerns the methods developed to isolate and partially characterize a major component of FhSmIII(M), a 12-kDa polypeptide, as well as immunity studies involving this antigen. Utilizing conventional gel filtration, followed by diethylaminoethyl (DEAE) Sephadex A-120 and monitoring the fractions by polyacrylamide gel electrophoresis (PAGE) and enzyme-linked immunoelectrotransfer blot techniques (EITB), we were able to isolate the 12-kDa antigenic polypeptide to homogeneity. Conventional gel filtration chromatography was followed by high-pressure, liquid anion, exchange chromatography, when highly purified material was needed, although the effective yields diminished drastically with the latter. Mice, rabbits and calves with a primary infection of F. hepatica developed antibodies (detectable in enzyme linked immunosorbent assay (ELISA) to the F. hepatica 12-kDa polypeptide within 2 weeks of infection. Mice with a primary infection of S. mansoni developed significant, but low, levels of anti-12-kDa antibodies by 7 weeks post-infection. Immunization of mice with microgram amounts of this 12-kDa polypeptide in Freunds' adjuvant resulted in the development of up to 77% less S. mansoni worms than the controls. Treatment with either endoglycosidase H, neuraminidase or dithiothreitol had no effect on the protein's mobility on sodium dodecyl sulfate (SDS)-PAGE or in its recognition by antibodies, suggesting the absence of carbohydrate moieties or disulphide bonds in relation to its antigenic determinants. Degradation by proteinase K further confirmed its polypeptide nature and points to recombinant DNA technology for the large-scale manufacture of this potential vaccine. Further use of this antigen in immunity studies should greatly contribute to the clarification of the mechanisms involved in cross-resistance against schistosomiasis. 相似文献
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The effect on the early inflammatory response in the bovine mammary gland of a factor extracted from the cell wall of Staphylococcus aureus was studied. The insoluble factor was extracted from a 3h-culture, and was mixed with S. aureus from an overnight culture (OF) for experimental infection of a lactating quarter. Other quarters were infected with a 3h or an overnight culture (O). Five cows were used in the experiment. The O-quarters showed more severe clinical signs than seen in the 3h-and OF-quarters. Antitrypsin levels and NAGase concentrations were much reduced in the 3h-and OF-quarters. While the cell wall factor profoundly reduced the early inflammatory response, the effect on the final outcome of the infection was not determined. 相似文献
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