全文获取类型
收费全文 | 153篇 |
免费 | 21篇 |
专业分类
林业 | 8篇 |
农学 | 8篇 |
18篇 | |
综合类 | 15篇 |
农作物 | 9篇 |
水产渔业 | 25篇 |
畜牧兽医 | 67篇 |
园艺 | 6篇 |
植物保护 | 18篇 |
出版年
2023年 | 2篇 |
2022年 | 7篇 |
2021年 | 1篇 |
2020年 | 6篇 |
2019年 | 8篇 |
2018年 | 13篇 |
2017年 | 13篇 |
2016年 | 7篇 |
2015年 | 5篇 |
2014年 | 6篇 |
2013年 | 18篇 |
2012年 | 7篇 |
2011年 | 13篇 |
2010年 | 9篇 |
2009年 | 6篇 |
2008年 | 4篇 |
2007年 | 3篇 |
2006年 | 1篇 |
2005年 | 7篇 |
2004年 | 1篇 |
2003年 | 6篇 |
2002年 | 3篇 |
2001年 | 2篇 |
1999年 | 1篇 |
1998年 | 3篇 |
1997年 | 4篇 |
1996年 | 3篇 |
1995年 | 2篇 |
1994年 | 3篇 |
1993年 | 3篇 |
1992年 | 1篇 |
1991年 | 3篇 |
1988年 | 1篇 |
1985年 | 1篇 |
1982年 | 1篇 |
排序方式: 共有174条查询结果,搜索用时 15 毫秒
121.
JR Strickland MA Custis AK Ashley LL Smith JL Klotz CR Krehbiel 《New Zealand veterinary journal》2013,61(5):288-292
AIM: To validate the use of para-aminohippuric acid (PAH) as a marker for measuring blood flow in wethers consuming a mixed diet of locoweed and blue grama hay. METHODS: Fourteen sheep, stratified by bodyweight (BW), were assigned to one of three treatments: 0.8 mg swainsonine (SW)/kg BW (HI), 0.2 mg SW/kg BW (LO), and no SW (Control). Sheep were fed various ratios of locoweed and blue grama hay to deliver SW treatments, for 28 days prior to infusion of PAH. Concentrations of SW and activities of alkaline phosphatase (Alk-P) and aspartate aminotransferase (AST) in serum were measured to confirm exposure to SW and subclinical intoxication. A single 20-ml injection of 5% PAH was delivered into the jugular vein after subclinical intoxication had been achieved. Blood samples were collected and serum analysed for PAH immediately prior to injection, then every 5 min from 5–30 min, and every 10 min from 30–60 min, following injection of PAH. RESULTS: Effective delivery of SW was evident from the greater concentrations of SW measured in the serum of HI compared with LO animals (p<0.05). No significant differences were detected in the rate of elimination (range 0.097–0.108 L/min), elimination half-life (range 6.62–7.24 min), apparent volume of distribution for the central compartment (range 7.14–9.72 L), and clearance (range 0.73–0.92 L/min) of PAH, between treatments. CONCLUSIONS: Subclinical intoxication with SW did not affect the pharmacokinetics of PAH. Thus, use of downstream dilution of PAH is a valid method to determine the rate of blood flow in nutrient flux experiments that involve consumption of locoweed. 相似文献
122.
Chaw Jiang Lim Mahiran Basri Dzolkhifli Omar Mohd Basyaruddin Abdul Rahman Abu Bakar Salleh Raja Noor Zaliha Raja Abdul Rahman 《Pest management science》2013,69(1):104-111
BACKGROUND: Pesticides are developed with carriers to improve their physicochemical properties and, accordingly, the bioefficacy of the applied formulation. For foliar‐applied herbicide, generally less than 0.1% of the active ingredient reaching the target site could reduce pesticide performance. Recently, a carrier of nanoemulsion consisting of oil, surfactant and water, with a particle size of less than 200 nm, has been shown to enhance drug permeability for skin penetration in pharmaceutical delivery systems. In the present work, the aim was to formulate a water‐soluble herbicide, glyphosate isopropylamine (IPA), using a green nanoemulsion system for a biological activity study against the weeds creeping foxglove, slender button weed and buffalo grass. RESULTS: The nanoemulsion formulations displayed a significantly lower spray deposition on creeping foxglove (2.9–3.5 ng cm?2), slender button weed (2.6–2.9 ng cm?2) and buffalo grass (1.8–2.4 ng cm?2) than Roundup® (3.7–5.1 ng cm?2). The visible injury rates of weeds treated with the nanoemulsion formulations were statistically equivalent to those relating to Roundup® at 14 days after treatment, with a control range of 86.67–96.67%. CONCLUSION: It was hypothesised that the significant difference in spray deposition with equal injury rates can be attributed to enhanced bioactivity of the nanoemulsion formulations. This initial discovery could be the platform for developing better penetration of agrochemical formulations in the future. Copyright © 2012 Society of Chemical Industry 相似文献
123.
124.
Sahu Amiya Ranjan V. Jeichitra R. Rajendran A. Raja 《Tropical animal health and production》2019,51(7):1817-1822
Tropical Animal Health and Production - The variability in breeding program leads to rapid loss of genetic potential for which National Bureau of Animal Genetic Resources is emphasized to conserve... 相似文献
125.
R Singh GS Sengar U Singh R Deb V Junghare S Hazra S Kumar S Tyagi AK Das TV Raja A Kumar 《Reproduction in domestic animals》2018,53(3):588-608
Male infertility is one of the prime concerns of dairy cattle production. The study was designed to find out differentially expressed proteins in categorized crossbred (Holstein Friesian × Sahiwal) bull semen to serve as potential biomarkers for male infertility. Frozen crossbred bull semen with satisfactory phenotypic records were defined as “good” and “poor” based on their fertility rates. A total of 1,547 proteins were detected in bull spermatozoa using liquid chromatography–mass spectrometer (LC‐MS/MS) analysis. Results revealed that 558 (36.1%) and 653 (42.2%) proteins were expressed to good and poor quality bull spermatozoa, respectively. A total of 336 proteins (21.7%) were reported to be unique for both good and poor quality bull semen, and among the common proteins, 224 (66.7%) and 112 (33.3%) were up‐ and downregulated in good and poor quality categorized bull semen, respectively. Gene Ontology analysis of global proteomes identified different signalling pathways, and most of them were related to cellular motility, immune systems as well as cellular metabolisms. The distinctive presence of some of the proteins may provide an insight into the molecular mechanistic role played by these proteins in crossbred bull infertility. 相似文献
126.
Comparison of in vitro and in vivo fertilizing potential of buffalo bull semen frozen in egg yolk‐, soya bean lecithin‐ and liposome‐based extenders
下载免费PDF全文
![点击此处可从《Reproduction in domestic animals》网站下载免费的PDF全文](/ch/ext_images/free.gif)
AK Singh A Kumar M Honparkhe S Kaur H Kaur SPS Ghuman PS Brar 《Reproduction in domestic animals》2018,53(1):195-202
The objective of this study was to compare different extenders for post‐thaw in vitro sperm function and in vivo fertility of buffalo semen. Accordingly, sperm of 30 ejaculates extended in egg yolk (TRIS with 20% egg yolk; EY), two soya lecithin‐based (SL‐1; AndroMed® and SL‐2; Bioxcell®) and a liposome‐based extender (LS; OptiXcell®) were tested. The post‐thaw semen was evaluated for computer‐assisted sperm analysis (CASA), sperm viability, membrane and acrosome integrity, DNA integrity and acrosome reaction and first service pregnancy rate (FSPR) in a fixed‐time artificial insemination programme. Total motility and VCL were the only CASA‐based parameters that exhibited significantly higher (p < .05) percentage in LS among these extenders. Post‐thaw percentage of acrosome integrity (55.9 ± 1.4, 58.1 ± 2.0, 55.8 ± 2.0, 56.6 ± 2.3) and DNA integrity (68.8 ± 2.0, 69.2 ± 2.3, 71.3 ± 2.1, 69.1 ± 2.1) did not differ (p > .05) in EY, SL‐1, SL‐2 and LS extender, respectively. However, a variable response in terms of efficacy of different extenders for sperm viability and plasma membrane integrity was observed. Assessment of inducibility of acrosome reaction showed significant differences between extenders (51.9 ± 2.1, 44.3 ± 2.4, 46.1 ± 2.3 and 58.1 ± 3.1%, respectively, for EY, SL‐1, SL‐2 and LS). Furthermore, field trials revealed significantly higher (p < .05) FSPR of LS‐extended semen as compared to that for EY, SL‐1 and SL‐2 extender (46.3%, 41.2%, 31.2% and 29.7%, respectively). It is concluded that the liposome‐based extender is more effective than egg yolk‐ and soya lecithin‐based extenders and may be used for cryopreservation of buffalo semen in the future. 相似文献
127.
Metabolism of the herbicide thiazopyr [methyl 2-(difluoromethyl)-5-(4, 5-dihydroO-lhiazo!vt)-4-(2-methylpropy!)-6-(trinuorornethyl)-3-pyridinecarboxy-late] was examined in young seedlings of redroot pigweed, grain sorghum, sunflower, corn and soybean. As previously observed with rat liver microsomes plants predominantly metabolized thiazopyr via oxidation reactions. Sulfur and carbon atoms in the thiazoline ring were the primary sites of plant oxygenases. De-esterification was also identified as an important pathway of transformations in plants. Although similar pathways of thiazopyr metabolism were observed among plants, our data indicated species differences in rates of thiazopyr degradation. Among species examined, pigweed (Amaranthus retroflexus L.) showed the fastest metabolism. Thiazopyr metabolism in pigweed was significantly inhibited by several cytochrome P450 monooxygenase inhibitors, among which tetcyclacis (TET) and piperonyl butoxide (PBO) were the most inhibitory. Thiazopyr metabolism in pigweed was not inhibited by organophosphates, known inhibitors of esterases. The results suggest that thiazopyr metabolism in plants is predominantly mediated via plant mono-oxygenases. 相似文献
128.
Chaw Jiang Lim Dzolkhifli OmarMohd. Basyaruddin Abdul Rahman Abu Bakar Salleh Raja Noor Zaliha Raja Abdul Rahman 《Industrial Crops and Products》2012,36(1):607-613
A nano-emulsion system was developed for pesticide formulation. Pseudoternary phase diagrams were constructed consisting emulsion system of long-chain fatty acid methyl esters (LFAMEs)/mixed surfactant/water and a quarternary component, glyphosate isopropylamine (IPA) as a herbicide active. Isotropic (L) regions were formed in the phase diagrams using mixed surfactant long-chain alkylpolyglucosides (LAPG) and ethoxylated 3-(3-hydroxypropyl)-heptamethyltrisiloxane (organosilicone) at the ratios of 9:1, 8:2 and 7:3. Pre-formulation concentrates were chosen from the L regions with less than 20% (w/w) of inerts (LFAMEs + mixed surfactant) and were characterized with regard to particle size, particle aging rate and thermostability study. A pre-formulation concentrate with the lowest aging rate and stable at high temperature (54 °C) was selected for the mechanisms study of the pre-formulation concentrate in conjunction with the development of nano-emulsion formulation. The transmission electron microscopy (TEM) result showed that the pre-formulation concentrate appeared as a polymerized multi-connected network. Upon water dilution of the pre-formulation concentrate with gentle stirring (low-energy emulsification method), well-dispersed nanoparticles were formed with no needle structure being observed. The nano-emulsion particles were incorporated well with the glyphosate IPA thus inferring that this nano-emulsion system could ameliorate the bioactivity and bioavailability of the herbicide. 相似文献
129.
Growth performance,nutrient digestibility and digestive enzyme activity in Asian seabass,Lates calcarifer juveniles fed diets supplemented with cellulolytic and amylolytic gut bacteria isolated from brackishwater fish
下载免费PDF全文
![点击此处可从《Aquaculture Research》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Asian seabass, Lates calcarifer juveniles were fed for 30 days to evaluate the efficacy of cellulolytic (Bacillus sp. DDKRC1.) and amylolytic (Bacillus subtilis DDKRC5.) bacteria supplemented diets. Four experimental groups were maintained in triplicate of which group I was the control group and received no bacterial supplementation. Groups II, III and IV were fed diet supplemented with B. subtilis DDKRC5. (14.25 × 107 CFU/mL), Bacillus sp. DDKRC1. (2.94 × 107 CFU/mL) and mixture of both the microbes (1:1 ratio), respectively, @ 1% (v/w). After 30 days of feeding, fish reared as group IV showed significantly higher (P < 0.01) weight gain (141.42%), survival (91.50%), protein efficiency ratio (1.18) and significantly lower (P < 0.01) FCR (2.15) than that of other groups. Nutrient digestibility parameters were significantly (P < 0.01) higher in group IV followed by groups III, II and I. Digestive cellulase, amylase and protease activities in the gastrointestinal tract were also significantly (P < 0.01) higher in group IV. Amylolytic and cellulolytic microbial count in the gastrointestinal (GI) tract were significantly (P < 0.01) higher in groups II, III and IV as compared with that of control. It can be concluded that feed supplemented with mixture of B. subtilis DDKRC5. and Bacillus sp. DDKRC1. showed better performance in Asian seabass juveniles. 相似文献
130.
The National Board of Veterinary Medical Examiners was interested in the possible effects of word count on the outcomes of the North American Veterinary Licensing Examination. In this study, the authors investigated the effects of increasing word count on the pacing of examinees during each section of the examination and on the performance of examinees on the items. Specifically, the authors analyzed the effect of item word count on the average time spent on each item within a section of the examination, the average number of items omitted at the end of a section, and the average difficulty of items as a function of presentation order. The average word count per item increased from 2001 to 2008. As expected, there was a relationship between word count and time spent on the item. No significant relationship was found between word count and item difficulty, and an analysis of omitted items and pacing patterns showed no indication of overall pacing problems. 相似文献