全文获取类型
收费全文 | 153篇 |
免费 | 21篇 |
专业分类
林业 | 8篇 |
农学 | 8篇 |
18篇 | |
综合类 | 15篇 |
农作物 | 9篇 |
水产渔业 | 25篇 |
畜牧兽医 | 67篇 |
园艺 | 6篇 |
植物保护 | 18篇 |
出版年
2023年 | 2篇 |
2022年 | 7篇 |
2021年 | 1篇 |
2020年 | 6篇 |
2019年 | 8篇 |
2018年 | 13篇 |
2017年 | 13篇 |
2016年 | 7篇 |
2015年 | 5篇 |
2014年 | 6篇 |
2013年 | 18篇 |
2012年 | 7篇 |
2011年 | 13篇 |
2010年 | 9篇 |
2009年 | 6篇 |
2008年 | 4篇 |
2007年 | 3篇 |
2006年 | 1篇 |
2005年 | 7篇 |
2004年 | 1篇 |
2003年 | 6篇 |
2002年 | 3篇 |
2001年 | 2篇 |
1999年 | 1篇 |
1998年 | 3篇 |
1997年 | 4篇 |
1996年 | 3篇 |
1995年 | 2篇 |
1994年 | 3篇 |
1993年 | 3篇 |
1992年 | 1篇 |
1991年 | 3篇 |
1988年 | 1篇 |
1985年 | 1篇 |
1982年 | 1篇 |
排序方式: 共有174条查询结果,搜索用时 15 毫秒
131.
Sahu Amiya Ranjan V. Jeichitra R. Rajendran A. Raja 《Tropical animal health and production》2019,51(7):1817-1822
Tropical Animal Health and Production - The variability in breeding program leads to rapid loss of genetic potential for which National Bureau of Animal Genetic Resources is emphasized to conserve... 相似文献
132.
Comparison of in vitro and in vivo fertilizing potential of buffalo bull semen frozen in egg yolk‐, soya bean lecithin‐ and liposome‐based extenders
下载免费PDF全文
![点击此处可从《Reproduction in domestic animals》网站下载免费的PDF全文](/ch/ext_images/free.gif)
AK Singh A Kumar M Honparkhe S Kaur H Kaur SPS Ghuman PS Brar 《Reproduction in domestic animals》2018,53(1):195-202
The objective of this study was to compare different extenders for post‐thaw in vitro sperm function and in vivo fertility of buffalo semen. Accordingly, sperm of 30 ejaculates extended in egg yolk (TRIS with 20% egg yolk; EY), two soya lecithin‐based (SL‐1; AndroMed® and SL‐2; Bioxcell®) and a liposome‐based extender (LS; OptiXcell®) were tested. The post‐thaw semen was evaluated for computer‐assisted sperm analysis (CASA), sperm viability, membrane and acrosome integrity, DNA integrity and acrosome reaction and first service pregnancy rate (FSPR) in a fixed‐time artificial insemination programme. Total motility and VCL were the only CASA‐based parameters that exhibited significantly higher (p < .05) percentage in LS among these extenders. Post‐thaw percentage of acrosome integrity (55.9 ± 1.4, 58.1 ± 2.0, 55.8 ± 2.0, 56.6 ± 2.3) and DNA integrity (68.8 ± 2.0, 69.2 ± 2.3, 71.3 ± 2.1, 69.1 ± 2.1) did not differ (p > .05) in EY, SL‐1, SL‐2 and LS extender, respectively. However, a variable response in terms of efficacy of different extenders for sperm viability and plasma membrane integrity was observed. Assessment of inducibility of acrosome reaction showed significant differences between extenders (51.9 ± 2.1, 44.3 ± 2.4, 46.1 ± 2.3 and 58.1 ± 3.1%, respectively, for EY, SL‐1, SL‐2 and LS). Furthermore, field trials revealed significantly higher (p < .05) FSPR of LS‐extended semen as compared to that for EY, SL‐1 and SL‐2 extender (46.3%, 41.2%, 31.2% and 29.7%, respectively). It is concluded that the liposome‐based extender is more effective than egg yolk‐ and soya lecithin‐based extenders and may be used for cryopreservation of buffalo semen in the future. 相似文献
133.
R Singh GS Sengar U Singh R Deb V Junghare S Hazra S Kumar S Tyagi AK Das TV Raja A Kumar 《Reproduction in domestic animals》2018,53(3):588-608
Male infertility is one of the prime concerns of dairy cattle production. The study was designed to find out differentially expressed proteins in categorized crossbred (Holstein Friesian × Sahiwal) bull semen to serve as potential biomarkers for male infertility. Frozen crossbred bull semen with satisfactory phenotypic records were defined as “good” and “poor” based on their fertility rates. A total of 1,547 proteins were detected in bull spermatozoa using liquid chromatography–mass spectrometer (LC‐MS/MS) analysis. Results revealed that 558 (36.1%) and 653 (42.2%) proteins were expressed to good and poor quality bull spermatozoa, respectively. A total of 336 proteins (21.7%) were reported to be unique for both good and poor quality bull semen, and among the common proteins, 224 (66.7%) and 112 (33.3%) were up‐ and downregulated in good and poor quality categorized bull semen, respectively. Gene Ontology analysis of global proteomes identified different signalling pathways, and most of them were related to cellular motility, immune systems as well as cellular metabolisms. The distinctive presence of some of the proteins may provide an insight into the molecular mechanistic role played by these proteins in crossbred bull infertility. 相似文献
134.
The National Board of Veterinary Medical Examiners was interested in the possible effects of word count on the outcomes of the North American Veterinary Licensing Examination. In this study, the authors investigated the effects of increasing word count on the pacing of examinees during each section of the examination and on the performance of examinees on the items. Specifically, the authors analyzed the effect of item word count on the average time spent on each item within a section of the examination, the average number of items omitted at the end of a section, and the average difficulty of items as a function of presentation order. The average word count per item increased from 2001 to 2008. As expected, there was a relationship between word count and time spent on the item. No significant relationship was found between word count and item difficulty, and an analysis of omitted items and pacing patterns showed no indication of overall pacing problems. 相似文献
135.
Chaw Jiang Lim Dzolkhifli OmarMohd. Basyaruddin Abdul Rahman Abu Bakar Salleh Raja Noor Zaliha Raja Abdul Rahman 《Industrial Crops and Products》2012,36(1):607-613
A nano-emulsion system was developed for pesticide formulation. Pseudoternary phase diagrams were constructed consisting emulsion system of long-chain fatty acid methyl esters (LFAMEs)/mixed surfactant/water and a quarternary component, glyphosate isopropylamine (IPA) as a herbicide active. Isotropic (L) regions were formed in the phase diagrams using mixed surfactant long-chain alkylpolyglucosides (LAPG) and ethoxylated 3-(3-hydroxypropyl)-heptamethyltrisiloxane (organosilicone) at the ratios of 9:1, 8:2 and 7:3. Pre-formulation concentrates were chosen from the L regions with less than 20% (w/w) of inerts (LFAMEs + mixed surfactant) and were characterized with regard to particle size, particle aging rate and thermostability study. A pre-formulation concentrate with the lowest aging rate and stable at high temperature (54 °C) was selected for the mechanisms study of the pre-formulation concentrate in conjunction with the development of nano-emulsion formulation. The transmission electron microscopy (TEM) result showed that the pre-formulation concentrate appeared as a polymerized multi-connected network. Upon water dilution of the pre-formulation concentrate with gentle stirring (low-energy emulsification method), well-dispersed nanoparticles were formed with no needle structure being observed. The nano-emulsion particles were incorporated well with the glyphosate IPA thus inferring that this nano-emulsion system could ameliorate the bioactivity and bioavailability of the herbicide. 相似文献
136.
137.
Growth performance,nutrient digestibility and digestive enzyme activity in Asian seabass,Lates calcarifer juveniles fed diets supplemented with cellulolytic and amylolytic gut bacteria isolated from brackishwater fish
下载免费PDF全文
![点击此处可从《Aquaculture Research》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Asian seabass, Lates calcarifer juveniles were fed for 30 days to evaluate the efficacy of cellulolytic (Bacillus sp. DDKRC1.) and amylolytic (Bacillus subtilis DDKRC5.) bacteria supplemented diets. Four experimental groups were maintained in triplicate of which group I was the control group and received no bacterial supplementation. Groups II, III and IV were fed diet supplemented with B. subtilis DDKRC5. (14.25 × 107 CFU/mL), Bacillus sp. DDKRC1. (2.94 × 107 CFU/mL) and mixture of both the microbes (1:1 ratio), respectively, @ 1% (v/w). After 30 days of feeding, fish reared as group IV showed significantly higher (P < 0.01) weight gain (141.42%), survival (91.50%), protein efficiency ratio (1.18) and significantly lower (P < 0.01) FCR (2.15) than that of other groups. Nutrient digestibility parameters were significantly (P < 0.01) higher in group IV followed by groups III, II and I. Digestive cellulase, amylase and protease activities in the gastrointestinal tract were also significantly (P < 0.01) higher in group IV. Amylolytic and cellulolytic microbial count in the gastrointestinal (GI) tract were significantly (P < 0.01) higher in groups II, III and IV as compared with that of control. It can be concluded that feed supplemented with mixture of B. subtilis DDKRC5. and Bacillus sp. DDKRC1. showed better performance in Asian seabass juveniles. 相似文献
138.
Enterocytozoon hepatopenaei (EHP) is a microsporidian parasite that causes hepatopancreatic microsporidiosis (HPM) in penaeid shrimp. HPM was observed in several countries, including Thailand and India; it has become a prominent pathogen in shrimp culture. Based on observations on EHP infection in the wild, the route of transmission has been hypothesized. Identification of artificial EHP infection procedures can facilitate our understanding of EHP transmission. Experimental transmission of EHP was attempted using the immersion and oral infections of infection. In the immersion mode, post‐larvae (PL) were exposed to an EHP tissue homogenate (0.2%) by immersion for 48 hr. Experimental samples were collected at various time points, and infection was confirmed using polymerase chain reaction, haematoxylin and eosin staining, transmission electron microscopy and modified trichrome staining. All test results revealed successful EHP transmission. Similar results were obtained through oral infection (oral infection). Innate immune gene expression patterns during infection were analysed; prophenoloxidase, crustin and superoxide dismutase were upregulated at 6, 6 and 48 hr post‐challenge, respectively. Experimental infection procedures facilitate the development of diagnostic and prevention strategies. This is the first study demonstrating the experimental transmission of EHP in shrimp PL. 相似文献
139.
140.