首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   221篇
  免费   36篇
  国内免费   15篇
林业   14篇
农学   10篇
基础科学   12篇
  24篇
综合类   45篇
农作物   4篇
畜牧兽医   155篇
园艺   1篇
植物保护   7篇
  2024年   1篇
  2023年   6篇
  2022年   5篇
  2021年   5篇
  2020年   8篇
  2019年   7篇
  2018年   7篇
  2017年   8篇
  2016年   15篇
  2015年   13篇
  2014年   15篇
  2013年   21篇
  2012年   10篇
  2011年   16篇
  2010年   3篇
  2009年   13篇
  2008年   9篇
  2007年   10篇
  2006年   8篇
  2005年   5篇
  2004年   5篇
  2003年   7篇
  2002年   9篇
  2001年   3篇
  2000年   3篇
  1999年   7篇
  1998年   10篇
  1997年   4篇
  1996年   6篇
  1995年   8篇
  1993年   3篇
  1992年   3篇
  1991年   1篇
  1990年   4篇
  1989年   3篇
  1988年   2篇
  1987年   2篇
  1986年   3篇
  1985年   1篇
  1984年   1篇
  1982年   1篇
  1980年   1篇
排序方式: 共有272条查询结果,搜索用时 15 毫秒
61.
Following a field campaign to determine the species composition, canopy cover, aboveground annual production and leaf area index (LAI) of the semi-arid savanna of north-western Namibia, we present a production model that can be used by graziers to determine the livestock carrying capacity. The model predicts the annual aboveground net primary production (ANPP) from regression equations of canopy cover by annual production fraction for plant functional classes. We tested the output of the model against another fully independent net primary production (NPP) model, namely the MODIS NPP product. The mean MODIS NPP for the 29 sites was 343 ± 22?kg dry matter (DM) ha?1 y?1 as opposed to 285 ± 142?kg DM ha?1 y?1 for the fAP model that used the regression method (p < 0.01). As a proof of concept, these landscape-scale ANPP values are used to calculate a recommended livestock carrying capacity for the Ehirovipuka Communal Conservancy, a 1 980 km2 communal area with both wildlife and livestock populations. In addition, we also provide details of a field method for predicting landscape-scale LAI from line transect data. This approach can be used to ground reference the LAI values generated from the MODIS LAI product.  相似文献   
62.
63.
64.
Wisent, or European bison (Bison bonasus), is listed as “vulnerable” on the IUCN Red List of Threatened Species and is therefore protected by international law. For the first time, a Wisent embryo has been obtained in vitro. This procedure creates a new opportunity to protect and increase Wisent reproductive potential and thereby opens new possibilities for the establishment of a controlled and broad reserve of the gene pool.  相似文献   
65.
 对四川省36个柑桔产区324个柑桔园的调查结果表明,柑桔慢衰线虫病 (Tylenchulus semipenetrans)在四川普遍发生,病情趋势是川东部盆地区发生最重,盆周低、中山区次之,川西南干热河谷较轻.果园平均发病率为87.4%,病情Ι级园占35.6%,Ⅱ级占24.6%,Ⅲ级占20.4%,Ⅳ级占6.7%.每年引起的经济损失达2.3%~45%;对18种柑桔品种田间发病情况调查结果:酒饼勒未见发病,香橼、江南柑、曼桔发病较轻,枳对该病抗性表现不一致,其余均严重感病,香橼、江南柑、曼桔发病较轻,枳对该病抗性表现不一致,其余均严重感病;T.semipenetrans田间群体密度周年消长规律研究结果,土中侵染幼虫群体密度以春、秋季最大,夏季最小;根上侵染幼虫群体密度以8月最小,秋、冬最大;而雌成虫全年在根上的发生量均低,而且变动幅度不大.  相似文献   
66.
马尾松林碳储量成熟问题初探   总被引:1,自引:0,他引:1  
应用国际上森林碳汇项目中普遍采用的碳计量方法,研究了湖南省不同立地条件的马尾松林碳储量变化过程,提出了碳储量成熟的定义,并分析了立地条件对碳储量成熟的影响,最后就碳储量成熟对于开展森林碳汇的作用进行了探讨。  相似文献   
67.
为评估不同种养密度和投喂频率对鱼菜共生系统中鲫鱼生长及品质的影响,建立温室基质栽培式鱼菜共生系统(鲫鱼+生菜),设置种植密度分别为28(A1)、42(A2)、56(A3)株·m-2;养殖密度分别为8(B1)、10(B2)、12(B3)kg·m-3;饲料投喂频率分别为1(C1)、2(C2)、3(C3)次·d-1,以明确鱼...  相似文献   
68.
The purpose of our work was to establish an efficient-oriented enucleation method to produce transgenic embryos with handmade cloning (HMC). After 41–42 h oocytes maturation, the oocytes were further cultured with or without 0.4 μg/ml demecolcine for 45 min [chemically assisted handmade enucleation (CAHE) group vs polar body (PB) oriented handmade enucleation (OHE) group respectively]. After removal of the cumulus cells and partial digestion of the zona pellucida, oocytes with visible extrusion cones and/or polar bodies attached to the surface were subjected to oriented bisection. Putative cytoplasts without extrusion cones or PB were selected as recipients. Two cytoplasts were electrofused with one transgenic fibroblasts expressing green fluorescent protein (GFP), while non-transgenic fibroblasts were used as controls. Reconstructed embryos were cultured in Well of Wells (WOWs) with porcine zygote medium 3 (PZM-3) after activation. Cleavage and blastocyst rates were registered on day 2 and day 7 of in vitro culture respectively. Meanwhile, the total blastocyst cell number was counted on day 7. We found that the difference was only observed between blastocyst rates (38.6 ± 2% vs 48.1 ± 3%) of cloned embryos with GFP transgenic fibroblast cells after CAHE vs OHE. With adjusted time-lapse for zonae-free cloned embryos cultured in WOWs with PZM-3, it was obvious that in vitro developmental competence after CAHE was compromised when compared with the OHE method. OHE enucleation method seems to be a potential superior alternative method used for somatic cell nuclear transfer (SCNT) with transgenic fibroblast cells.  相似文献   
69.
Oestrogen receptor alpha (ERalpha), the main subtype in the uterus, is involved in the regulation of uterine growth/proliferation. A relationship between ERalpha and proliferative activity has been shown in the cyclic sow uterus, but to our knowledge, no study has been carried out on early pregnant sows. Therefore, by means of immunohistochemistry and use of mouse monoclonal antibodies to ERalpha and a proliferative marker, Ki-67, the localization of these proteins was investigated in the sow uterus during early pregnancy. Eighteen crossbred multiparous sows were artificially inseminated once at 20-15 h before expected ovulation. After artificial insemination (AI), they were slaughtered at five different times: at oestrus, 5-6 h after AI (n = 4), 20-25 h after ovulation (n =4), 70 h after ovulation (n = 4), on day 11 (the first day of standing oestrus = day 1, n = 3) and on day 19 (n = 3). Immediately after slaughter, uterine samples were collected at the mesometrial side of the uteri, fixed in 10% formaldehyde and embedded in paraffin. Immunohistochemistry was performed by using mouse monoclonal antibodies to ERalpha (C-311) and Ki-67 (MM1). All sows slaughtered after ovulation were pregnant. In general, positive immunostaining for ERalpha and Ki-67 was found in the nuclei. Variations in staining intensity and proportion of positive nuclei were observed in different uterine compartments and stages of early pregnancy. The highest level of ERalpha presence in the surface epithelium and myometrium was found at oestrus (5-6 h after AI), and low levels of ERalpha in these compartments were observed as early as 20-25 h after ovulation. In the glandular epithelia, presence of ERalpha was highest at 70 h after ovulation. The largest number of ERalpha-positive cells in the stroma was observed at oestrus and early after ovulation. Low proliferation was observed, and with no significant difference in tissue compartments except in the glandular epithelium. High proliferative activity in the glandular epithelium at 70 h after ovulation indicated involvement in preparation for secretory activity and growth during pregnancy establishment. Significant positive correlation was found between the number of ERalpha-positive cells in the stroma and Ki-67-positive cells in the surface epithelium. In conclusion, the present study showed differences in immunolocalization of ERalpha and the proliferative marker Ki-67 in different tissue compartments of the sow uterus at oestrus and early pregnancy. In some uterine compartments, the patterns of ERalpha and Ki-67 immunostaining seemed to be influenced by insemination and the presence of embryos, in addition to the effects of steroid hormones.  相似文献   
70.
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号