Diagnosis of botulism in cattle using ELISA to detect antibody to botulinum toxins   总被引：1，自引：0，他引：1  

TF JUBB  TM ELLIS  AR GREGORY 《Australian veterinary journal》1993,70(6):226-227

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稻草床栽草菇培养料优质发酵方法研究   总被引：1，自引：0，他引：1  

纪开萍  何明霞  阿红昌  王文兵  张春霞 《食用菌学报》2007,14(1):32-36

简易发酵、一次发酵和二次发酵稻草培养料床栽草菇的试验结果表明,二次发酵法栽培的草菇生物学效率达16.3%,显著高于一次发酵及简易发酵,且能较好地控制病虫害的发生,提高了草菇的产量和质量。  相似文献   

Evaluation of Fertilizing Potential of Frozen-thawed dog Spermatozoa Diluted in ACP-106^® using an In Vitro Sperm–Oocyte Interaction Assay     

RCS Cardoso  AR Silva  LDM Silva  VH Chirinéa  FF Souza  MD Lopes 《Reproduction in domestic animals》2007,42(1):11-16

The aim of present study was to evaluate frozen canine semen with ACP-106 (Powder Coconut Water) using an in vitro sperm--oocyte interaction assay (SOIA). Ten ejaculates from five stud dogs were diluted in ACP-106 containing 20% egg yolk, submitted to cooling in a thermal box for 40 min and in a refrigerator for 30 min. After this period, a second dilution was performed using ACP-106 containing 20% egg yolk and 12% glycerol. Samples were thawed at 38 degrees C for 1 min. Post-thaw motility was evaluated by light microscopy and by using a computer aided semen analysis (CASA). Plasma membrane integrity and sperm morphology/acrosomal status were evaluated by fluorescent probes (C-FDA/PI) and Bengal Rose respectively. Moreover, frozen-thawed semen was analysed by a SOIA. Subjective post-thaw motility was 52.0 +/- 14.8% and it was significant higher than the total motility estimated by CASA (23.0 +/- 14.8%) because this system considered the egg yolk debris as immotile spermatozoa. Although normal sperm rate and acrosomal integrity evaluated by Bengal Rose stain was 89.6 +/- 3.1% and 94.3 +/- 3.1%, respectively, post-thaw percentage of intact plasma membrane was only 35.1 +/- 14.3%. Regarding SOIA, the percentage of interacted oocytes (bound, penetrated and bound and/or penetrated) was 75.3%. Using regression analysis, it was found significant relations between some CASA patterns and data for SOIA. In conclusion, the freezing-thawing procedure using ACP-106 was efficient for maintain the in vitro fertility potential of dog spermatozoa.  相似文献   

Weeds resistant to chlorsulfuron and atrazine from the north-east grain region of Australia     

S. W. ADKINS  D. WILLS  M. BOERSMA  S. R. WALKER  G. ROBINSON  R. J. MCLEOD  J. P. EINAM 《Weed Research》1997,37(5):343-349

The resistance of weeds to triazine and sulfonylurea herbicides has been recorded in several countries. The extent of the problem in the north-east grain region of Australia is uncertain. In an initial study, resistance to chlorsulfuron and atrazine in 15 weed species was investigated. The study showed that at least six of them have evolved resistance to one or other of these herbicides. Two collections of Rapistvum rugosum L., three of Sisymbrium orientale L., five of Sonchus oleraceus L., one of Fallopia convolvulus (L.) A. Love and one of Sisymbrium rhellungii O. Schultz were resistant to the recommended rate of chlorsulfuron (15 g a.i. ha^-1). Resistance to chlorsulfuron was not discovered in three collections of Sisymbrium L., two of Brassica tournefortii Gouan. three of Emex australis Steinh. and 11 of Phalaris paradoxa L. Two collections of Urochtoa panicoides Beauv. were resistant, and three collections possibly resistant, to the recommended rate of atrazine(1.8 kg a.i. ha^-1). Resistance to atrazine was not found in 12 collections of Hibiscus urionum L., nine of Salvia reflexa L., two of Parthenium hysterophorus L., two of Amaranthus viridis L. and 14 of Echinochloa colona (L) Link. The resistance status of four weeds ( R. rugosum. F. convolvulus, S. thellungii and U. panicoides was confirmed using a multiple dose-response screen and is the first report of resistance for these species. Herbicide usage records show that resistance has developed after 3-10 years of selection with chlorsulfuron and 2-15 years of selection with atrazine, with no correlation between the frequency of use and the degree of resistance for any of the species where eight or more collections were made.  相似文献   

SNP g.1007A>G within the porcine DNAL4 gene affects sperm motility traits and percentage of midpiece abnormalities          下载免费PDF全文

I Wiedemann  A Maehlmeyer  S Jansen  AR Sharifi  C Knorr 《Reproduction in domestic animals》2018,53(2):401-413

The flagellar beating of a spermatozoa's axoneme is caused by the varying activation and inactivation of dynein molecules. Dynein, axonemal, light chain 4 (DNAL 4 ) is a functional candidate gene for sperm motility as it encodes a small subunit of the dyneins. We resequenced the porcine DNAL 4 using three artificial insemination (AI ) boars each with high (>68%) or low (<60%) motility, and detected 23 SNP . These were then genotyped for 82 AI boars. Using spermatological records, significantly negative genetic correlations between ejaculate volume (VOL ) and the further spermatological parameters concentration (CONC ) (r  = ?.43), motility of undiluted semen (MOTUD ) (r  = ?.09), motility after 24 h (MOT 1) (r  = ?.17) and after 48 hr (MOT 2) (r  = ?.23) were estimated. Significantly positive correlations existed between CONC and MOT 1 (r  = .07) as well as MOT 2 (r  = .10), between MOTUD and MOT 1 (r  = .33), between MOTUD and MOT 2 (r  = .36), and finally between MOT 1 and MOT 2 (r  = .70). Significantly negatively correlated were all motility traits with the parameters abnormal acrosome (AA ) (MOTUD r  = ?.06; MOT 1 r  = ?.08, and MOT 2 r  = ?.1) and presence of cytoplasmic droplet (CD ) (MOTUD r  = ?.07; MOT 1 r  = ?.08; MOT 2 r  = ?.07). Association analyses (single marker regression model; SMR ) propose that SNP g.1007A>G, located in the second intron, reduces motility significantly (MOTUD ‐4.59%; MOT 1 ‐10.33%; MOT 2 ‐19.37%). According to the dominant‐recessive model (DRM ), genotype AA is always superior compared to genotypes AG and GG (i.e. MOTUD 67.67%, 64.16% and 53.91%; MOT 1 54.17%, 43.75% and 28.44%; MOT 2 44.12%, 24.91% and 4.97%). The average effect of gene substitution (g.1007A>G) on abnormal midpiece (AM ) was 0.71%, the genotypic values—as expressed by LS means—were 0.1 (AA ) and 0.81 (AG ).  相似文献   

Qualitative and quantitative ultrasound attributes of maternal‐foetal structures in pregnant ewes          下载免费PDF全文

PDA da Silva  RAR Uscategui  VJC Santos  AR Taira  RSG Mariano  MGK Rodrigues  APR Simões  MC Maronezi  ML Avante  WRR Vicente  MAR Feliciano 《Reproduction in domestic animals》2018,53(3):725-732

The aim of this study was to examine foetal organs and placental tissue to establish a correlation between the changes in the composition of these structures associated with their maturation and the ultrasonographic characteristics of the images. Twenty‐four pregnant ewes were included in the study. Ultrasonography assessments were performed in B‐mode, from the ninth gestational week until parturition. The lungs, liver and kidneys of foetuses and placentomes were located in transverse and longitudinal sections to evaluate the echogenicity (hypoechoic, isoechoic, hyperechoic or mixed) and echotexture (homogeneous and heterogeneous) of the tissues of interest. For quantitative evaluation of the ultrasonographic characteristics, it was performed a computerized image analysis using a commercial software (Image ProPlus^®). Mean numerical pixel values (NPVs), pixel heterogeneity (standard deviation of NPVs) and minimum and maximum pixel values were measured by selecting five circular regions of interest in each assessed tissue. All evaluated tissues presented significant variations in the NPVs, except for the liver. Pulmonary NPVmean, NPVmin and NPVmax decreased gradually through gestational weeks. The renal parameters gradually decreased with the advancement of the gestational weeks until the 17th week and later stabilized. The placentome NPVmean, NPVmin and NPVmax decreased gradually over the course of weeks. The hepatic tissue did not show echogenicity and echotexture variations and presented medium echogenicity and homogeneous echotexture throughout the experimental period. It was concluded that pixels numerical evaluation of maternal‐foetal tissues was applicable and allowed the identification of quantitative ultrasonographic characteristics showing changes in echogenicity related to gestational age.  相似文献   

Factors affecting the breeding success of the African Black Oystercatcher (Haematopus moquini): a perspective on protection and food availability     

Douglas Loewenthal  Philip AR Hockey 《African Zoology》2016,51(4):193-202

Breeding success (fledglings pair^?1 y^?1) of the Red-listed African Black Oystercatcher (Haematopus moquini) is highly variable, both spatially and temporally. Despite a diversity of natural factors causing this variability, there is evidence that two anthropogenic factors, i.e. disturbance and an introduced mussel (Mytilus galloprovincialis), are having an impact on the local breeding success of this species. Using a data set comprising 87 site-years of nest-monitoring data across most of the species’ breeding range, we analysed the extent and causes of variability in breeding success. Breeding success differed across three population categories defined by varying levels of human disturbance: island populations, protected mainland populations, and unprotected mainland populations. Differences in breeding success between island populations and protected mainland populations were likely due to differing exposure to predators; however, differences between protected and unprotected mainland populations were unlikely caused by this as both experience equivalent predation levels (although from different predators). Protection only improved the breeding success of oystercatchers in very high-quality habitats (with a high biomass of alien mussels), and where populations were ‘released’ from high levels of human disturbance. In unprotected mainland areas, human activity impacted on the breeding success of local populations primarily through predation of small chicks by uncontrolled dogs, and by rising tides drowning chicks that were hiding from human disturbance. The findings of this study note the potential conservation dilemma resulting from an invasive species improving the conservation status of a Red-listed species, and encourage the implementation of restricted sites in high-quality habitats with high breeding pair densities.  相似文献   

Monitoring the reproductive physiology of six‐banded armadillos (Euphractus sexcinctus,Linnaeus, 1758) through different techniques          下载免费PDF全文

LB Campos  GCX Peixoto  GL Lima  TS Castelo  AM Silva  CIA Freitas  AR Silva 《Reproduction in domestic animals》2016,51(5):736-742

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Use of phytotoxic rice crop residues for weed management     

SOPHEA PHENG  MARIA OLOFSDOTTER  GARY JAHN  STEVE ADKINS 《Weed Biology and Management》2010,10(3):176-184

There is a general perception among Cambodian rice (Oryza sativa) farmers that, after harvesting, rice crop residues that are incorporated into the field benefit the growth of the subsequent rice crop. However, the effect of this action upon weed establishment and growth has not yet been considered. A series of pot and field trials were conducted to determine whether such action could inhibit weed establishment and/or growth. The pot studies first evaluated the response of the test plant (rice line ST‐3) and three weed species, barnyardgrass (Echinochloa crus‐galli), small umbrella sedge (Cyperus difformis), and water primrose (Ludwigia octovalves), to the residue of 16 rice lines and the field trials were later conducted to evaluate the response of the same test plants to the residue of seven putatively allelopathic rice lines and one non‐allelopathic rice line. The residue of all the studied rice lines, depending on how long they had been incorporated into the soil, reduced the establishment and growth of all three weed species, as well as the rice crop. However, if the residue's incorporation was delayed by 2 weeks or only a proportion of the residue was incorporated, the rice crop could withstand the growth‐inhibiting effect, while the inhibition of the establishment and growth of the three weed species was retained. These responses of rice and the weeds to rice crop residues might provide a basis for a weed management strategy, particularly in the resource‐poor rice‐production systems of Cambodia.  相似文献   

Epidemic of blindness in kangaroos - evidence of a viral aetiology     

PT HOOPER  RA LUNT  AR GOULD  AD HYATT  GM RUSSELL  JA KATTENBELT  SD BLACKSELL  LA REDDACLIFF  PD KIRKLAND  RJ DAVIS  PJK DURHAM  AL BISHOP  J WADDINGTON 《Australian veterinary journal》1999,77(8):529-536

OBJECTIVE: To determine the cause of an epidemic of blindness in kangaroos. DESIGN AND PROCEDURES: Laboratory examinations were made of eyes and brains of a large number of kangaroos using serological, virological, histopathological, electron microscopical, immunohistochemical methods, and PCR with cDNA sequencing. In addition, potential insect viral vectors identified during the disease outbreak were examined for specific viral genomic sequences. SAMPLE POPULATION: For histopathological analysis, 55 apparently blind and 18 apparently normal wild kangaroos and wallabies were obtained from New South Wales, Victoria, South Australia, and Western Australia. A total of 437 wild kangaroos and wallabies (including 23 animals with apparent blindness) were examined serologically. RESULTS: Orbiviruses of the Wallal and Warrego serogroups were isolated from kangaroos affected with blindness in a major epidemic in south-eastern Australia in 1994 and 1995 and extending to Western Australia in 1995/96. Histopathological examinations showed severe degeneration and inflammation in the eyes, and mild inflammation in the brains. In affected retinas, Wallal virus antigen was detected by immunohistochemical analysis and orbiviruses were seen in electron microscopy. There was serological variation in the newly isolated Wallal virus from archival Wallal virus that had been isolated in northern Australia. There were also variations of up to 20% in genotype sequence from the reference archival virus. Polymerase chain reactions showed that Wallal virus was present during the epidemic in three species of midges, Culicoides austropalpalis, C dycei and C marksi. Wallal virus nucleic acid was also detected by PCR in a paraffin-embedded retina taken from a blind kangaroo in 1975. CONCLUSION: Wallal virus and perhaps also Warrego virus are the cause of the outbreak of blindness in kangaroos. Other viruses may also be involved, but the evidence in this paper indicates a variant of Wallal virus, an orbivirus transmitted by midges, has the strongest aetiological association, and immunohistochemical analysis implicates it as the most damaging factor in the affected eyes.  相似文献