Comparison of Different Glycerol and Egg Yolk Concentrations Added to Tris‐based Extender for the Collared Peccaries (Tayassu tajacu) Semen Freezing     

HM Alves  IRS Oliveira  TS Castelo  GL Lima  ALP Souza  MAP Moreira  VV de Paula  AR Silva 《Reproduction in domestic animals》2013,48(3):506-511

This study aimed to evaluate various concentrations of egg yolk (5, 10, or 20%) in combination with different concentrations of glycerol (3% or 6%) added to a Tris‐based extender on the post‐thaw characteristics of sperm obtained from Tayassu tajacu. For this purpose, semen from 10 sexually male mature collared peccaries was collected by electroejaculation and evaluated for sperm motility, vigour, viability, morphology and functional membrane integrity. The ejaculates were initially extended in Tris‐fructose plus egg yolk (5%, 10% or 20%). After cooling, the semen was added to Tris‐egg yolk plus glycerol (6% or 12%), resulting in a final concentration of 3% or 6% glycerol of the extender. Straws were frozen using liquid nitrogen and thawed in a water bath at 37°C for 30 s. The frozen–thawed semen was evaluated as reported for fresh semen. After thawing, a significant decrease was verified for sperm motility and vigour, for all the samples in comparison with fresh semen. However, no differences were evidenced among treatments for any sperm characteristics evaluated (p > 0.05), except for the combination between 10% egg yolk and 6% glycerol, which provided the worst preservation of functional membrane integrity (p < 0.05). The interactions between higher concentrations of egg yolk (20%) and glycerol (6%) and also between lower concentrations of the same substances (5% egg yolk and 3% glycerol) added to the Tris‐based extender negatively affected the preservation of the normal sperm morphology after thawing (p < 0.05). In conclusion, the use of Tris‐based extender added to 10% or 20% egg yolk plus 3% glycerol is recommended for effective sperm cryopreservation in collared peccaries.  相似文献   

The development of multiple cutaneous inverted papilloma following ovariohysterectomy in a dog     

JS Munday  AF French  AR MacNamara 《New Zealand veterinary journal》2013,61(3):168-171

Abstract

CASE HISTORY: Ovariohysterectomy was performed on an adult Cavalier King Charles Spaniel. The skin that had been clipped for surgery was noticed to be erythematous 8 days later.

CLINICAL AND PATHOLOGICAL FINDINGS: Poorly defined patches containing multiple papules were visible bilaterally within the clipped skin. These became larger over the following 2 weeks, and samples were collected for histology. Seven days later, the lesions were multiple raised masses, up to 5 cm in diameter. Histology revealed numerous cup-shaped epidermal proliferations extending into the dermis. The presence of keratinocytes with increased quantities of blue-grey cytoplasm, and koilocytosis suggested papillomavirul infection; Canis familiaris papillomavirus (CfPV-2) DNA was amplified from two separate samples. Complete regression was observed 8 weeks after the lesions had been initially observed.

DIAGNOSIS: Multiple inverted papilloma confined to skin that had been clipped for surgery.

CLINICAL RELEVANCE: This is the first time that the development of canine cutaneous papillomas has been associated with surgery. The nature of the association between surgery and development of the papillomas is uncertain. However, it is possible that damage to superficial skin could promote the formation of papillomas. This is the first identification of CfPV-2 in New Zealand.  相似文献   

Increased Risk of Conception Failure in German Holstein Friesian Cows with Chronic Endometritis     

G Tsousis  AR Sharifi  M Hoedemaker 《Reproduction in domestic animals》2010,45(6):1114-1117

The objective of this retrospective field study was to evaluate the effect of chronic clinical endometritis (CCE) on days open in German Holstein Friesian cows. Two hundred and sixty‐four cows diagnosed with CCE from day 14 to 42 postpartum were included in this study. A Cox’s proportional hazards regression model with stepwise forward selection for days open was used and the variables type of vaginal discharge, ovarian cysts and a marked loss of body condition score proved to be significant with hazard ratios of 1.5, 2.0 and 1.9 respectively. Although not remaining in the model, a weak positive effect of treatment for CCE and a negative effect of high milk production could also be found in the univariate analysis. These results suggest that the suppression of fertility of animals diagnosed with CCE varies according to the different symptoms, which could be used to support decisions regarding treatment. Management factors such as a marked loss in body condition and high milk production prolonged the number of days open of animals with CCE.  相似文献   

菜籽粕可替代含可溶物酒糟饲喂泌乳奶牛     

Mulrooney C N  Schingoethe D J  Kalscheur K F  Hippen A R  张树金 《饲料博览》2010,(5):49-50

试验旨在确定饲喂含菜籽粕作蛋白质添加物替代全部或部分含可溶物酒糟日粮的反应。试验采用4×4拉丁方设计,12头泌乳荷斯坦奶牛(4头初产、8头经产),试验期4周,在第3和第4周收集数据。配合日粮时,添加菜籽粕100%、66%、33%、0分别替代相应数量的玉米酒糟可溶物(DDGS)。所有日粮含饲草55%(其中玉米青贮50%、苜蓿干草50%)、精料45%,平均含粗蛋白15.1%、乙醚抽提物4.5%。试验结果表明,所有日粮的干物质采食量(25.4kg·d-1)相似。所有日粮的产奶量相似,添加菜籽粕100%、66%、33%、0日粮的产奶量分别为35.2、35.8、34.5、34.3kg·d-1,但菜籽粕含量高的日粮产奶量较高。所有日粮乳蛋白率(3.04%)、乳脂率(3.92%)和乳脂量(1.37kg·d-1)相似,而乳蛋白量则随着日粮中菜籽粕含量的增加而提高,100%、66%、33%、0菜籽粕日粮分别为1.08、1.10、1.05、1.03kg·d-1。所有日粮的饲料转化率相似,每千克干物质采食量含能量校正乳1.46kg。饲喂菜籽粕或DDGS时,赖氨酸是乳蛋白合成的第一限制性氨基酸,而菜籽粕和DDGS同时饲喂时,蛋氨酸为第一限制性氨基酸。所有日粮的瘤胃内容物氨和挥发性脂肪酸浓度相似。试验结果表明,在奶牛日粮中,菜籽粕是DDGS的一种适宜替代物。  相似文献   

Relationship between milk production and somatic cell count in dairy cows in East Gippsland     

AR CAMERON  GA ANDERSON 《Australian veterinary journal》1993,70(1):13-17

SUMMARY The analysis of records for 75 821 herd tests on 23 700 cows in the Macalister Irrigation District of East Gippsland, using a model that also included stage of lactation, herd and cow effects, found a strong relationship between somatic cell count and milk production. Unit increases in the natural logarithm of the somatic cell count were associated with decreases in production of up to 2.6% in the range 54 598 to 403 429 cells per mL. This is equivalent to a decrease of 1.9% for a doubling of cell count from 100 000 to 200 000 cells per mL. Subclinical mastitis was assessed to be a significant source of loss in milk production in the study area.  相似文献   

Pyrrolizidine alkaloid poisoning in yaks. First report and confirmation by identification of sulphurbound pyrrolic metabolites of the alkaloids in preserved liver tissue     

H. WINTER  AA SEAWRIGHT  AR MATTOCKS  R. JUKES  U. TSHEWANG  BJ GURUNG 《Australian veterinary journal》1990,67(11):411-412

  相似文献   

A modified ELISA for the detection of goats infected with Mycobacterium paratuberculosis   总被引：1，自引：0，他引：1  

AR MILNER  WN MACK  KJ COATES 《Australian veterinary journal》1989,66(9):305-307

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Pyrrolizidine alkaloid poisoning of yaks: diagnosis of pyrrolizidine alkaloid exposure by the demonstration of sulphur-conjugated pyrrolic metabolites of the alkaloid in circulating haemoglobin     

H WINTER  AA SEAWRIGHT  J HRDLICKA  AR MATTOCKS  R JUKES  KINZANG WANGDI  KB GURUNG 《Australian veterinary journal》1993,70(8):312-313

  相似文献   

Use of enzyme-linked immunoassays for antibody to types C and D botulinum toxins for investigations of botulism in cattle   总被引：3，自引：0，他引：3  

AR GREGORY  TM ELLIS  TF JUBB†  RJ NICKELS‡  DV COUSINS 《Australian veterinary journal》1996,73(2):55-61

The development of specific enzyme-linked immunosorbent assays (ELISA) for antibody to types C and D Clostridium botulinum toxins for investigation of botulism in cattle is described. Partially purified type C and D toxins were used as antigens to develop these ELISAs. Specificity of the ELISAs was evaluated on sera from 333 adult beef and dairy cattle from areas with no history or evidence of botulism in animals or water birds. The test was also evaluated on sera from 41 herds that included herds vaccinated against botulism, confirmed botulism cases and herds from areas where the disease is considered endemic. The ELISAs detected the presence of antibody to botulinum toxins in samples from vaccinated cattle and both convalescent and clinically normal animals from unvaccinated herds with outbreaks of botulism. Antibody was also found in unvaccinated animals from herds in which there had been no diagnosed botulism cases in areas where botulism was considered endemic. Sera from some unvaccinated cattle with high ELISA reactivity was shown to be protective for mice in botulinum toxin neutralisation tests. The use of these tests in investigations of botulism in cattle is discussed.  相似文献   

An evaluation of calf castration by intra-testicular injection of a lactic acid solution   总被引：1，自引：0，他引：1  

G. FORDYCE  NJ BEAMAN  AR LAING  PB HODGE†  C. CAMPERO†  RK SHEPHERD¶ 《Australian veterinary journal》1989,66(9):272-276

This experiment evaluated intra-testicular injection of a sclerosing drug, lactic acid, for castration of bulls. Its use was compared in 58 Brahman cross calves (50 to 128kg) with the general practice of open surgical castration. Chemical castration appeared to be more painful than surgical castration, though post-operative swelling and pain appeared similar for both methods. Chemical castration took 3 times longer than surgical castration (58 sec v 20 sec; P less than 0.01). Scrotal necrosis occurred in 25% of chemically-castrated calves and appeared due to drug leakage from the testes under the high pressure of injection. Healing time for chemical castrates was approximately twice that for surgical castrates. Five chemically-castrated calves (18%) retained one testis. Though all 5 were rendered sterile, each maintained androgenesis. This led to secondary male behaviour which caused management problems. Castration method did not influence post-operative growth. It is concluded that lactic acid administration is not a suitable alternative to the open surgical technique for castration of Brahman cross calves.  相似文献