Isolation in cell cultures and initial characterisation of two novel bocavirus species from swine in Northern Ireland   总被引：1，自引：0，他引：1  

McKillen J  McNeilly F  Duffy C  McMenamy M  McNair I  Hjertner B  Millar A  McKay K  Lagan P  Adair B  Allan G 《Veterinary microbiology》2011,152(1-2):39-45

We report the isolation in cell cultures of two novel bocavirus species in pigs from farms in Northern Ireland with clinical postweaning multisystemic wasting syndrome (PMWS). We have designated the isolates as porcine bocavirus-3 (PBoV3) and porcine bocavirus-4 (PBoV4). To date 5082 and 4125 bps of PBoV3 and PBoV4 have been sequenced, respectively. PBoV3 and PBoV4 show nucleotide homology to other known bocaviruses in swine and other organisms. Open reading frame (ORF) analysis has shown that these viruses have a third small ORF, equivalent to the NP1 ORF that distinguishes the bocaviruses from other parvoviruses. A panel of porcine field sera was screened by indirect immunofluorescence against both viruses. Of the 369 samples analysed, 32 (8.7%) and 35 (9.5%) sera were seropositive for PBoV3 and PBoV4 respectively, thus providing serological evidence of the exposure of swine in the field to bocavirus-like viruses. To date, the clinico-pathological significance of these novel swine bocaviruses, as primary pathogens or as immunosuppresive triggers for other infectious agents, is undetermined.  相似文献   

Pathological pigmentation in cardiac tissues of Atlantic salmon (Salmo salar L.) with cardiomyopathy syndrome     

Hilde AS Fagerland  Lars Austb?  Camilla Fritsvold  Marta Alarcon  Espen Rimstad  Knut Falk  Torunn Taksdal  Erling O Koppang 《Veterinary research》2013,44(1):107

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Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products     

Tom N McNeilly  Mara Rocchi  Yvonne Bartley  Jeremy K Brown  David Frew  Cassandra Longhi  Louise McLean  Jenni McIntyre  Alasdair J Nisbet  Sean Wattegedera  John F Huntley  Jacqueline B Matthews 《Veterinary research》2013,44(1):70

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The effect of Psoroptes ovis infestation on ovine epidermal barrier function     

Miriam R Stoeckli  Tom N McNeilly  David Frew  Edward J Marr  Alasdair J Nisbet  Adri HM van den Broek  Stewart TG Burgess 《Veterinary research》2013,44(1):11

Sheep scab is an intensively pruritic, exudative and allergic dermatitis of sheep caused by the ectoparasitic mite Psoroptes ovis. The purpose of the present study was to investigate the effect of P. ovis infestation on different components of the ovine epidermal barrier within the first 24 hours post-infestation (hpi). To achieve this, the expression of epidermal differentiation complex (EDC) genes and epidermal barrier proteins, the nature and severity of epidermal pathology and transepidermal water loss (TEWL) were evaluated.By 1 hpi a significant dermal polymorphonuclear infiltrate and a significant increase in TEWL with maximal mean TEWL (598.67 g/m²h) were observed. Epidermal pathology involving intra-epidermal pustulation, loss of epidermal architecture and damage to the basement membrane was seen by 3 hpi. Filaggrin and loricrin protein levels in the stratum corneum declined significantly in the first 24 hpi and qPCR validation confirmed the decrease in expression of the key EDC genes involucrin, filaggrin and loricrin observed by microarray analysis, with 5.8-fold, 4.5-fold and 80-fold decreases, respectively by 24 hpi.The present study has demonstrated that early P. ovis infestation disrupts the ovine epidermal barrier causing significant alterations in the expression of critical barrier components, epidermal pathology, and TEWL. Many of these features have also been documented in human and canine atopic dermatitis suggesting that sheep scab may provide a model for the elucidation of events occurring in the early phases of atopic sensitisation.  相似文献   

Long‐term control of olfactory neuroblastoma in a dog treated with surgery and radiation therapy          下载免费PDF全文

E Gumpel  AS Moore  DJ Simpson  KL Hoffmann  DP Taylor 《Australian veterinary journal》2017,95(7):227-231

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Genetic screening of FecB,FecXG and FecXI mutations and their linkage with litter size in Barki and Rahmani sheep breeds          下载免费PDF全文

AS El‐Seedy  NM Hashem  KM El‐Azrak  ANM Nour El‐Din  TA Ramadan  TA Taha  MH Salem 《Reproduction in domestic animals》2017,52(6):1133-1137

Characterization of fecundity genes offers the opportunity to improve production efficiency, and the consequent increase in litter size in livestock industry, through utilizing them in breeding programs. The main objective of this study was to detect the BMPR‐IB, BMP15 and GDF9 gene mutations and to investigate whether these mutations are associated with litter size in Egyptian sheep breeds. To achieve this goal, 73 adult ewes representing Barki (n = 33) and Rahmani (n = 40) breeds were used. Polymerase chain reaction–restriction fragment length polymorphism (PCR‐RFLP) screening approach was used to detect the presence of FecB, FecX^G and FecX^I mutations in the two selected breeds. Results of this study showed that the three different candidate gene mutations, namely FecB, FecX^G and FecX^I are not present among these selected populations of the Egyptian breeds. Further studies regarding other mutations and/or other genes, which may influence ovulation rate, should be carried out to determine the type and mode of inheritance of such genes in Egyptian sheep breeds.  相似文献   

Activation of the immune system is the pivotal event in the production of wasting disease in pigs infected with porcine circovirus-2 (PCV-2)   总被引：39，自引：0，他引：39  

Krakowka S  Ellis JA  McNeilly F  Ringler S  Rings DM  Allan G 《Veterinary pathology》2001,38(1):31-42

Porcine circovirus (PCV)-2, a newly described single-stranded circular DNA virus pathogen of swine is the cause of postweaning multisystemic wasting syndrome (PMWS). In gnotobiotic piglets, PCV-2 infection alone produces asymptomatic infection without evidence of overt PMWS. Gnotobiotic piglets infected with PCV-2 were injected with keyhole limpet hemocyanin in incomplete Freund's adjuvant (KLH/ICFA), and the effects on virus production and development of PMWS were determined. In the first experiment, piglets were injected subcutaneously on the left hip and shoulder, and viral burden was assessed in regional lymph nodes draining the injection sites and in contralateral lymph nodes 13-14 days after infection. Immune activation increased the number of virus antigen-positive cells in draining lymph nodes and increased the amount of infectious virus recovered by 1-4 log10. In a second experiment, the effects of injections of KLH/ICFA with or without concurrent stimulation of peritoneal macrophages by intraperitoneal injections of thioglycollate broth on induction of PMWS was assessed. All immunized piglets developed moderate to severe PMWS, whereas none of the piglets infected with PCV-2 alone developed PMWS. In PMWS-affected piglets, extensive replication of PCV-2 was documented by both immunocytochemistry and quantitative viral titrations. Thus, immune activation is a key component of the pathogenesis of PCV-2-associated PMWS in swine.  相似文献   

Association of porcine circovirus 2 with reproductive failure in pigs: a retrospective study, 1995-1998   总被引：7，自引：0，他引：7       下载免费PDF全文

Bogdan J  West K  Clark E  Konoby C  Haines D  Allan G  McNeilly F  Meehan B  Krakowka S  Ellis JA 《The Canadian veterinary journal. La revue veterinaire canadienne》2001,42(7):548-550

In order to determine if vertically transmitted porcine circovirus (PCV) has played a role in reproductive failure in pigs in areas of endemic infection, archival fixed tissues were examined by polymerase chain reaction (PCR) and immunohistochemistry. Tissues tested were from routine cases of abortion or reproductive failure submitted between 1995 and 1998 to the diagnostic laboratory at the Western College of Veterinary Medicine, Saskatoon. They originated from 29 high-health herds in the provinces of Alberta and Saskatchewan and comprised a total of 36 individual submissions. Porcine circovirus type 1 (PCV1) was not detected by PCR in any submitted tissues. Porcine circovirus type 2 (PCV2) was not detected by PCR or immunohistochemistry in any of the submitted tissue. The effect of extended formalin fixation on the detection of PCV2 by PCR was assessed and fixation for up to one week had no gross effect on sensitivity of detection using this PCR technique. Failure to detect porcine circoviruses in cases of reproductive failure prior to 1999 in areas of endemic infections, suggests that reproductive disease may be a new clinical manifestation of PCV2 infection, and that vertical transmission may not have been the primary mechanism of initial dissemination of the virus in the pig population.  相似文献   

Effect of Days Post-Partum, Breed and Ovum Pick-Up Scheme on Bovine Oocyte Recovery and Embryo Development     

AS Lopes  T Martinussen  T Greve  H Callesen 《Reproduction in domestic animals》2006,41(3):196-203

The objective of this study was to investigate (i) the effect of two different ovum pick-up (OPU) schemes (once vs twice weekly aspirations) on oocyte recovery rate, quality and subsequent in vitro embryo development, (ii) the influence of days post-partum on oocyte recovery and (iii) possible differences in OPU results from two different herds. In group A, OPU was performed twice weekly in two Holstein Friesian (HF) and three Danish Red and White (DRW) cows from a private herd. In the research herd, two groups of eight HF cows were investigated: group B (OPU once weekly) and group C (OPU twice weekly). The collected oocytes were subsequently submitted to in vitro embryo production. More oocytes were recovered from the private herd when compared with the research herd. In the research herd, the twice weekly scheme aspirated more oocytes than the once weekly scheme. The quality of the retrieved oocytes was significantly different between groups B and C but not between groups A and C, and HF cows yielded higher quality oocytes than DRW cows (p = 0.029). Oocytes from group C showed higher level of embryonic development than group B oocytes. No differences in blastocyst rates were observed between groups A and C. Session affected the number of retrieved oocytes and subsequent developmental rates, with these being lower in the first compared with the last sessions. Finally, there was no significant effect of days post-partum in the number and quality of the retrieved oocytes, likely because of the small group size and high variation between sessions.  相似文献   

Rapid detection of porcine reproductive and respiratory syndrome viral nucleic acid in blood using a fluorimeter based PCR method   总被引：4，自引：0，他引：4  

Spagnuolo-Weaver M  Walker IW  Campbell ST  McNeilly F  Adair BM  Allan GM 《Veterinary microbiology》2000,76(1):15-23

Porcine reproductive and respiratory syndrome virus (PRRSV) is an Arterivirus recognised world wide as an important cause of reproductive failure and pneumonia in pigs. American and European strains of PRRSV, differentiated antigenically and genomically, have been reported. PRRSV infections are currently diagnosed using serology, virus isolation and/or immunocytochemistry. In order to overcome various drawbacks associated with these techniques, conventional, block-based RT-PCR methods for the detection of PRRSV nucleic acid in clinical samples have been described. These methods require gel electrophoresis for analysis of PCR products and present high risk of DNA carry-over contamination between the samples tested. We describe the detection of PRRSV RNA in serum samples and in blood impregnated filter disks (FDs), obtained from experimentally inoculated pigs, using a closed-tube, fluorimeter-based PCR assay. The assay eliminates the use of gel electrophoresis, and is as sensitive and specific as the conventional block-based PCR assay, detecting positive samples as early as 1 day post-inoculation. We also report a rapid fluorimeter based PCR method for differentiating American and European strains of PRRSV.  相似文献