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Adriano do N. Simes Marília C. Ventrella Celso L. Moretti Marcelo A.G. Carnelossi Rolf Puschmann 《Postharvest Biology and Technology》2010,55(1):45-52
This study characterizes dehydration and white blush processes, structural alterations and synthesis of phenolic compounds (lignin and suberin), in relation to development of white blush on baby carrot surfaces. Carrots were minimally processed as baby carrots and kept on polypropylene trays with or without polyvinyl chloride (PVC) film at 5 ± 2 °C, 90 ± 5% RH. During storage, baby carrots that were not wrapped with PVC film were rehydrated 1, 1.5, 15 and 17 h after minimal processing. Fresh-cut baby carrots were evaluated for white blush index, sensory analysis (visual scores), fresh matter loss, phenylalanine ammonia-lyase (PAL) activity and structural and histochemical changes. Increases in white blush index and subjective visual scores on the carrot surface occurred in the first hours, when the material was kept on trays without PVC film and after 3 and 6 d, when stored on trays covered with PVC film. Visual assessment of white blush resulted in a more accurate assessment than instrumental evaluation because it allowed the perception of minor differences between distinct white blush levels, especially at the tips. Hydrating baby carrot surfaces for 1 and 1.5 h after processing allowed partial absorption of water by tissues and the orange color was reestablished on the surface. Fifteen hours after processing, even after rehydration, the color did not return to the original orange standard. The rapid increase in PAL activity in the secondary phloem of baby carrots, compared to that of whole carrots, suggested a physiological response as a result of abrasion. Dehydration and structural alterations of the more superficial cell layers were the main causes of white blush in baby carrots that was not related to lignin accumulation, but rather to synthesis of non-structural phenolic compounds. 相似文献
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M. Láday Á. Juhász G. Mulè A. Moretti Á. Szécsi A. Logrieco 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(5-6):545-550
Restriction fragment length polymorphisms (RFLPs) were used to assess genetic diversity of mitochondrial DNA (mtDNA) among standard isolates of seven lineages of Fusarium graminearum. The mtDNA patterns within each lineage were very similar (>89%), whereas significant differences were observed between the isolates belonging to different lineages, with the exception of lineages 1 and 4 where strong similarity was found between the RFLPs. Analysis of different band patterns resulted in characteristic HhaI and HaeIII bands that were suitable for identification of members of lineages 7, 6, 5, 3 and 2. Investigation of lineage distribution of 144 European isolates revealed that 142 belong to lineage 7. These data, therefore, confirmed the hypothesis that members of lineage 7 are predominant in Europe. Further analysis of isolates belonging to lineage 7 resulted in five haplotypes. These haplotypes have arisen as different combinations of three RFLP patterns for both HaeIII and HhaI restriction enzymes. Two isolates from Hungary, however, shared the same mtDNA RFLP profiles with a standard isolate of lineage 3, indicating that members of lineage 3, at a lower frequency, may also occur in Europe. 相似文献
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V do Lago WL Vianna RD Gama A de Campos Rosseto ME Pinese A de Sant''Anna Moretti 《Reproduction in domestic animals》2005,40(2):141-144
The use of exogenous gonadotrophins in puberty inducement and ovulation synchronization is a technique that has a positive influence on the management of swine. The purpose of this study was to verify the effects of a second gonadotrophin treatment [equine chorionic gonadotrophin (eCG) and luteinizing hormone (LH), intramuscularly (i.m.)] upon the second oestrus synchronization and fertility in gilts. Seventy-one NAIMA (Pen Ar Lan) gilts had their first oestrus (puberty inducement) induced by a hormonal treatment (eCG and LH). Then, they were randomly distributed into two treatments, with (T1) and without (C) gonadotrophin treatment at the second oestrus. The animals were fed with a single ration (16% of crude protein and 3286.73 kcal ME/kg), and timed artificial insemination performed at the second oestrus. Gilts were slaughtered for embryo recovery and ovary examination about 5 days after insemination. There was no evidence of a difference in the percentage of the second oestrus expression (T1 - 90.90% and C - 86.84%), the duration of the oestrus cycle (T1 - 19.62 +/- 0.82 days and C - 19.67 +/- 4.14 days), the percentage of follicular cysts (T1 - 15.15% and C - 18.42%) and number of ovulations (T1 - 14.60 +/- 5.7 and C - 13.23 +/- 4.8) between treatments (p > 0.05). However, the hormonal treatment (T1) showed minor oestrus dispersion and embryo viability (T1 - 8.4 +/- 5.6 and C - 11.2 +/- 4.6) (p < 0.05). These results indicate that the better synchronization and expression of the second oestrus when using gonadotrophins (eCG and LH) is followed by a lower embryo viability, which is probably the consequence of the heterogeneous follicle recruitment during the injection of eCG. 相似文献
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Cacciò SM Antunovic B Moretti A Mangili V Marinculic A Baric RR Slemenda SB Pieniazek NJ 《Veterinary parasitology》2002,106(4):285-292
The morphologically small Babesia species isolated from naturally infected dogs in Europe, Japan, and US are described as Babesia gibsoni despite the fact that molecular techniques show that they should be assigned to two or three separate taxons. The morphologically large Babesia isolated from dogs in Europe, Africa, and US were generally classified as B. canis until it was proposed to distinguish three related, albeit genetically distinct subspecies of this genus, namely B. canis canis, B. canis rossi, and B. canis vogeli. The insight into the molecular taxonomy of canine piroplasms is, however, limited because only partial small subunit ribosomal RNA (ssrRNA) sequence data exist for two species from the B. canis group. In this work, we molecularly characterised natural Babesia infections in 11 dogs from Croatia, France, Italy, and Poland. These infections were diagnosed as caused by B. canis canis and B. canis vogeli based on the analysis of the complete sequence of the ssrRNA genes. Phylogenetic analysis confirmed that the large Babesia species of dogs belong the to the Babesia sensu stricto clade, which includes species characterised by transovarial transmission in the tick vectors and by exclusive development inside the mammalian host erythrocytes. The new data facilitate the reliable molecular diagnosis of the subspecies of B. canis. 相似文献
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A. Moretti A. Belisario A. Tafuri A. Ritieni L. Corazza A. Logrieco 《European journal of plant pathology / European Foundation for Plant Pathology》2002,108(7):661-666
Fourty-four strains of Fusarium oxysporum were isolated from plants of melon with Fusarium wilt symptoms. Among these strains, thirty-nine were characterized for their pathogenicity on melon. Thirty-seven strains belonged to known races of F. oxysporum f. sp. melonis, while two strains were non-pathogenic. Four strains belonged to race 0, seven to race 1, four to race 2, and twenty-two to race 1,2. Beauvericin was produced by thirty-six strains in a range from 1 to 310gg–1. Eight isolates of race 1,2 did not produce the toxin. In addition, of the two non-pathogenic strains, only one strain produced the toxin (290gg–1). The production of enniatin A1, enniatin B1, and enniatin B was also investigated. Enniatin B was the only enniatin detected, being produced by eleven strains belonging to all the races, with a range of production from traces to 60gg–1. Finally, melon fruits belonging to two different cultivars (Cantalupo and Amarillo) were artificially inoculated with one strain of F. oxysporum f. sp. melonis (ITEM 3464). Beauvericin was detected in the fruit tissues of both cultivars at a level of 11.2 and 73.8gg–1, respectively. These data suggest that the production of both the toxins is not related to the pathogenicity of F. oxysporum f. sp. melonis, nor to the differential specificity of the races. The results confirm that beauvericin is a common metabolite of phytopathogenic Fusarium species. 相似文献
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X. -M. Xu D. W. Parry P. Nicholson M. A. Thomsett D. Simpson S. G. Edwards B. M. Cooke F. M. Doohan J. M. Brennan A. Moretti G. Tocco G. Mule L. Hornok G. Giczey J. Tatnell 《European journal of plant pathology / European Foundation for Plant Pathology》2005,112(2):143-154
Two years of field sampling aimed to establish the predominance and association among the fungal pathogens causing Fusarium ear blight (FEB) in four European countries (Hungary, Ireland, Italy and the UK). A PCR-based method was used to detect four Fusarium species and two varieties of Microdochium nivale present in the samples. The prevalence of FEB pathogens differed significantly between countries. Overall, all pathogens were commonly detected in Ireland and to a lesser extent in the UK. In contrast, only two species, F. graminearum and F. poae, were regularly detected in Italy and Hungary. Fusarium culmorum was rarely detected except in Ireland. Log-linear models were used to determine whether there is the independence of the six FEB pathogens at each sampling site. Significant two-pathogen interactions were frequently observed, particularly in harvest samples; all these significant two-pathogen interactions were of the synergistic type, except between F. poae and F. culmorum, and were generally consistent over the 2 years and four countries. Fusarium graminearum and F. poae were least frequently involved in two pathogen interactions but were involved in most of the nine significant three-pathogen interactions. However, only the interaction between F. graminearum, F. avenaceum and F. poae was significant in both years. Potential implications of the present results in FEB management are discussed. 相似文献