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SUMMARY The BACTEC radiometric culture method for detection of Mycobacterium paratuberculosis was evaluated on faeces from cattle on a farm in quarantine for Johne's disease. A multiplex polymerase chain reaction (PCR) based on the IS900 sequence specific for M paratuberculosis and a genus specific 16S rRNA region was developed and used to test cultures showing evidence of mycobacterial growth in the BACTEC liquid radiometric culture medium. Using the BACTEC - PCR combination, confirmation of M paratuberculosis from faeces and tissue of clinically affected animals was achieved within 2 to 4 weeks and 1 week, respectively, a substantial improvement on traditional culture and identification methods. The PCR provided rapid exclusion of M paratuberculosis when other Mycobacterium spp were grown. The radiometric culture medium proved to be very sensitive for culturing Mycobacterium spp. 相似文献
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F. Reda A. Raafat M. A. Abdel-Halim M. El-Kadi Z. Yacob 《Journal of Agronomy and Crop Science》1986,157(4):235-244
The effect of natural air temperature on the vegetative growth and alkaloidal yield of H. muticus L. was studied. The highest number of branches, diameter, fresh and dry weights of stem and roots occurred in September sowing at full-flowering and fruiting stages. Relatively higher temperature in Sept. sowing favoured leaf growth (number, area and dry weight). Hyoscyamine yield in different organs of the plants sown in Sept., excessively exceeded all other sowings during flower budding and full-flowering stages. Hyoscine yield was quantitatively very small as compared with that of hyoscyamine and even could not be detected in the leaves of winter sowings during full-flowering stage. 相似文献
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Abdel-Halim Ali EL-DARAWANY Ibrahim Fayez Mahmud MARAI Kamal Mohamed ELWAN Akram Abdel-Sattar EL-TARABANY 《Animal Science Journal》2005,76(6):541-547
In the present study, 48 crossbred does (Nubian × Baladi) were used to investigate physiological (mucus and hormonal) traits as affected by gestation pattern (pre‐, full‐ and post‐term parturitions). The incidence of full‐term (does with a gestation length of 146–152 days) was the highest (54.5%), followed by pre‐term (does with a gestation length of 140–145 days; 36.9%) and post‐term (does with a gestation length of 153 days; 8.9%), respectively. Prediction of gestation pattern by using some body measurements was also studied. Cervical mucus samples were collected during estrus to determine spinnbarkeit (i.e. strength, viscosity or stretchability) and pH values of the mucus. Mucus was also collected pre‐parturition daily from day 140 of gestation until delivery, to evaluate the ferning arborization. Blood samples were collected on the day before mating (0 time), 1 (at mating), 30, 60, 90 and 145 days after mating. The results showed that spinnbarkeit and pH in cervical mucus at estrus, serum estradiol‐17α (E2) at day 5 before delivery, and progesterone (P4) levels differed insignificantly according to gestation pattern. The fern degree between day 140 of gestation and day of delivery was the highest in pre‐term does, while it was the lowest in post‐ and full‐term does. The gestation length was the highest in post‐term does (157.5 days), and the lowest in pre‐term does (141.5 days). During winter, the incidence of pre‐term deliveries was the highest (54.5%), but in spring, full‐term deliveries were the most common (58.3%). Most of the traits studied were insignificantly affected by season of birth. Post‐term delivery can be estimated by doe weight and wither height, while full‐term delivery can be anticipated using by hip height and abdomen girth. Pre‐term does can be detected by cervical mucus fern between day 140 of gestation and day of delivery. Post‐term does can be defined by doe weight and wither height, while full‐term deliveries can be defined by hip height and abdomen girth, during pregnancy. 相似文献
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Decreased proportion of CD4+ and CD8+ T lymphocytes in peripheral blood likely contributed to susceptibility to Pneumocystis carinii in a foal. Cytological evaluation of bronchoalveolar lavage was required for identification of the pathogen and serial flow-cytometric analysis of peripheral blood lymphocytes documented transient low expression of CD4+ and CD8+ T lymphocytes. Although immunodeficiency is uncommon, it must be included in the differential diagnosis for patients suffering from chronic or opportunistic infections and may provide an indication for immunostimulant therapy. 相似文献
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Influence of xylazine on the function of the LiDCO sensor in isoflurane anaesthetized horses 
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下载免费PDF全文 Klaus Hopster Tamas D Ambrisko Jessica Stahl Johannes P Schramel Sabine BR Kästner 《Veterinary anaesthesia and analgesia》2015,42(2):142-149
ObjectivePrevious studies showed an influence of xylazine on the LiDCO sensor in vitro and in standing horses, but did not prove that this interaction caused error in LiDCO measurements. Therefore, agreement of cardiac output (CO) measurements by LiDCO and bolus-thermodilution (BTD) was determined in horses receiving xylazine infusions.Study designProspective, experimental study.AnimalsEight Warmblood horses.MethodsAll horses were premedicated with xylazine. Anaesthesia was induced with midazolam and ketamine and was maintained with isoflurane in oxygen. During six hours of anaesthesia CO measurements and blood samples were taken before, during and after a 60 minute period of xylazine infusion. Pairs of LiDCO and bolus thermo-dilution (BTD) measurements of CO were performed. Sensor voltages exposed to blood and saline were measured before, during and after xylazine infusion and compared using Bland-Altman method of agreement with corrections for repeated measures.ResultsThe CO values (mean ± SD) before xylazine were 34.8 ± 7.3 and 36.4 ± 8.1 L minute−1 for BTD and LiDCO, respectively. After starting the xylazine infusion, the CO values for BTD decreased to 27.5 ± 6.1 L minute−1 whereas CO values measured by LiDCO increased to 54.7 ± 18.4 L minute−1. One hour after discontinuing xylazine infusion, CO values were 33 ± 6.7 and 36.5 ±11.9 L minute−1 for BTD and LiDCO, respectively. The difference between saline and blood exposed sensor voltages decreased during xylazine infusion and these differences were positive numbers before but negative during the infusion. There were correlations between xylazine plasma concentrations, CO differences and sensor voltage differences (saline – blood).Conclusions and clinical relevanceThis study proved that xylazine infusion caused concentration dependent bias in LiDCO measurements leading to an overestimation of readings. Sensor voltage differences (saline – blood) may become valuable clinical tool to predict drug-sensor interactions. 相似文献
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ABSTRACT Aims: To compare the outcome, in terms of lameness score or return to athletic function, of horses with acute vs. chronic digital lameness that underwent magnetic resonance imaging (MRI) of the distal limb and to compare the proportion of horses that received intra-articular therapy of the distal interphalangeal (DIP) joint and pattern of diagnostic analgesia in these groups. Methods: This is a retrospective study of horses (n?=?95) with acute (≤12 weeks; n?=?46) or chronic (>12 weeks; n?=?49) digital lameness that underwent MRI of the distal limb from 2009–2016, at two equine referral centres in the USA. Criteria for inclusion in the study were that a majority of lameness localised distal to the fetlock, and that lameness assessments for ≥12 months following MRI could be obtained from the medical record or the owner could be interviewed regarding their horse's athletic function. Outcome was characterised by an improvement score where 2?=?return to work at a previous or higher level or lameness improved by one grade or more, 1?=?return to work at a lower level or lameness improved by less than one grade, and 0?=?did not return to work or lameness grade worsened. Whether horses had received intra-articular therapy of the DIP joint and the pattern of diagnostic analgesia prior to MRI was also obtained from medical records or by interviewing the owner. Results: There was a difference (p?=?0.004) in the proportion of horses assigned to improvement scores of 0, 1 and 2 between horses with acute or chronic lameness. There was no evidence of a difference in the likelihood of having received intra-articular therapy of the DIP joint prior to MRI between horses with chronic or acute lameness (p = 0.085). Similarly, there was no evidence of a difference in the pattern of diagnostic analgesia prior to MRI between the two groups (p = 0.94). Eighty-two percent of owners of horses with acute and 62% of those with horses with chronic lameness had a positive opinion of the utility of MRI as a diagnostic modality. Conclusion: In a population of horses with digital lameness undergoing MRI, a difference in the outcome, in terms of lameness score or return to athletic function was identified between horses with acute lameness compared to those with chronic lameness. Clinical relevance: Horses with digital lameness that undergo MRI when the lameness is acute may have an improved prognosis due to accurate diagnosis and earlier application of appropriate therapy. 相似文献
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Jozsef RáTKY Istvan EGERSZEGI Peter TOTH Soukanh KEONUCHAN Takashi NAGAI Kazuhiro KIKUCHI Noboru MANABE Klaus-Peter BRüSSOW 《The Journal of reproduction and development》2013,59(5):437-441
Worldwide, only a few “fatty” pig breeds exist with different and/or regional
utilization. Using the Hungarian Mangalica, which almost went extinct in Europe and the
Lao Moo Lat pig, which still has a large population in South-East Asia as exemples, we
wanted to demonstrate that indigenous (fatty) pig breeds may represent both national value
and tremendous economic potential. Since these less prolific and less productive breeds
cannot contribute to mass production, new market roles and methods should be established
for them in the premium segment of pork trading. Thus their preservation and propagation
needs the comprehensive collaboration of commercial, governmental actors and researchers.
Briefly summarizing the history, we report the current results of reproductive physiology
research. The commercial renaissance of Mangalica pigs is indebted to the enthusiastic
efforts of basic scientists, pig breeding experts and dedicated Mangalica producers.
Scientific achievements were applied to practical breeding and production of delicious
pork and processed products, which ultimately made the economic success in the Mangalica
sector possible. Both, research on and utilization of endangered (pig) breeds maintain not
only breed diversities, but also may improve the livelihood of farmers worldwide. 相似文献