Immunolocalisation of desmoglein-1 in equine muzzle skin     

Miragliotta V  Donadio E  Felicioli A  Podestà A  Ricciardi MP  Ceccardi S  Abramo F 《Equine veterinary journal》2006,38(5):485-487

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Differentiation between field and vaccine strain of bluetongue virus serotype 16     

Monaco F  Cammà C  Serini S  Savini G 《Veterinary microbiology》2006,116(1-3):45-52

In August 2000, bluetongue virus (BTV) appeared for the first time in Sardinia and, since then, the infection spread across Sicily and into the mainland of Italy involving at the beginning serotypes 2 and 9 and then, from 2002, 4 and 16. To reduce direct losses due to disease and indirect losses due to new serotype circulation, the 2004 Italian vaccination campaign included the modified-live vaccines against BTV-4 and 16 produced by Onderstepoort Biological Product (OBP), South Africa. Few months after the end of the campaign, BTV-16 was reported broadly in the country and the need of differentiating field from the BTV-16 vaccine isolate became crucial. In this study, the gene segments 2, 5, 6 and 10 of both the Italian and vaccine BTV-16 strains were sequenced and their molecular relationship determined. As sequences of segment 5 were those showing the highest differences (17.3%), it was possible to develop a new diagnostic tool able to distinguish the Italian BTV-16 NS1 gene from that of the homologous vaccine strain. The procedure based on the use of a RT-PCR and the subsequent sequencing of the amplified product showed a high degree of sensitivity and specificity when samples from either BTV-16 vaccinated or infected sheep were tested.  相似文献   

LC-MS study to reduce ion suppression and to identify N-lactoylguanosine 5'-monophosphate in bonito: a new umami molecule?     

de Rijke E  Ruisch B  Bakker J  Visser J  Leenen J  Haiber S  de Klerk A  Winkel C  König T 《Journal of agricultural and food chemistry》2007,55(16):6417-6423

In this study a specific taste modulating flavor ingredient, N-lactoylguanosine 5'-monophosphate (N-lactoyl GMP), was determined in bonito (Japanese, Katsuobushi, dried fermented skipjack) and in powdered bonito using liquid chromatography-electrospray ionization (+) mass spectrometry-mass spectrometry (LC-ESI(+)-MS/MS) with a methanol/ammonium acetate or formate gradient. Furthermore, the influence of ion suppression due to sample matrix effect was investigated and was found to substantially influence the total MS response of N-lactoyl GMP; by adjusting the LC conditions the response could be approximately 5-fold-enhanced. The N-lactoyl GMP concentrations in different types of bonito products were between 0.2 and 2.4 microg/g.  相似文献   

Extraction and formation dynamic of oak-related volatile compounds from different volume barrels to wine and their behavior during bottle storage   总被引：1，自引：0，他引：1  

Pérez-Prieto LJ  López-Roca JM  Martínez-Cutillas A  Pardo-Mínguez F  Gómez-Plaza E 《Journal of agricultural and food chemistry》2003,51(18):5444-5449

The extraction rate of furfuryl aldehydes, guaiacol, and 4-methylguaiacol, cis- and trans-oak lactone, and vanillin and the formation rate of furfuryl alcohol and the volatile phenols 4-ethylguaiacol and 4-ethylphenol have been studied in wines matured in different capacity oak barrels (220, 500, and 1000 L). Also, the behavior of these compounds during 1 year of wine bottle storage was followed. The lactones were extracted at a linear rate with large differences that depended on barrel volume. Those compounds related to oak toasting (guaiacol, 4-methylguaiacol, furfuryl aldehydes, and vanillin) seemed to be extracted faster during the first days of oak maturation except for vanillin, which required at least 3 months to accumulate in the wine. The volatile phenols, 4-ethylphenol and 4-ethylguaiacol, were formed in large quantities after the first 90 days of oak maturation, coinciding with the end of spring and beginning of summer. Wines matured in 1000-L oak barrels resulted in the lowest levels of volatile compound accumulation. During bottle storage, some compounds decreased in their concentration (5-methylfurfural, vanillin), others experienced increases in their levels (lactones, furfural, 4-ethylguaiacol, 4-ethylphenol), and the concentration of other compounds hardly changed (guaiacol, furfuryl alcohol).  相似文献   

Foam aptitude of trepat and monastrell red varieties in cava elaboration. 1. Base wine characteristics     

Girbau-Solà T  López-Tamames E  Buján J  Buxaderas S 《Journal of agricultural and food chemistry》2002,50(20):5596-5599

The foam properties of base wines made from red autochthonous varieties (Trepat and Monastrell) were studied. Four wines of each variety were elaborated (fermented off skins at industrial scale in two consecutive harvests) and blended at different proportions with the white traditional variety (Macabeo, Xarel.lo, and Parellada) wines to elaborate Cava (closed-bottle-fermented sparkling wine). When crescent amounts of Trepat were added to the traditional white blend, the foamability and the color intensity (CI) of the wine increased polinomically. The increase of the CI depended on the year of harvest. Thus, oenologists could decide the blend proportion most suitable to elaborate either a "blanc de noirs" sparkling wine or a new type of Cava.  相似文献   

Synthesis of the sednet work package 3 outcomes     

Damià Barceló  Mira PetroYic  Eric de Deckere  Joop F. Bakker  Mark Scrimshaw  Julia Stegemann  Grazyna Kowalewska  Peter Heininger  Carlos Vale  Peter Diedrieh Hansen  María Jesús Belzunce Segarra  Andrew Parker  Véronique Poulsen  Werner Manz  Juliän Blasco  Ángel DelValls Casillas 《Journal of Soils and Sediments》2004,4(4):223-224

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Milk let-down in goats can be influenced by the type of stimuli applied or to which goats are exposed during suckling and hand milking     

Teodulo González-Quirino  José Alberto Delgadillo  José Alfredo Flores  Jesús Vielma  Gerardo Duarte  Manuel de Jesús Flores  Angélica Terrazas  Luis Angel Zarazaga  Alexis Adrián Vargas-Cruz  Ricardo Avilés  Horacio Hernández 《Animal Science Journal》2021,92(1):e13646

The present experiments on goats were conducted with the aim of verifying (a) whether the identity of the goat kids can affect the characteristics of milk let-down during suckling (Study 1) and (b) whether the presence of the goat kids may improve the milk let-down during hand milking (Study 2). In Study 1, 16 lactating goats with twins and 12 with single kids were used. Dams with single goat kids ejected more milk when suckling their kids at Days 5 and 20 of lactation, than when suckling aliens. Dams with twins ejected more milk when suckling their own kids than aliens only at Day 5 of lactation. Taking into account all the dams, they ejected more milk with more fat, protein, and lactose during suckling their own litter than when suckling aliens. In Study 2, when dams (n = 13) were hand milked in the presence of their own litter or in its absence, they ejected more milk with more fat, protein, and lactose than when milked in the presence of alien kids. We conclude that milk let-down in goats can be influenced by the type of stimuli applied or to which they are exposed to during suckling and milking.  相似文献   

Butterfly biodiversity in the city is driven by the interaction of the urban landscape and species traits: a call for contextualised management     

Pla-Narbona  Cl&#;udia  Stefanescu  Constant&#;  Pino  Joan  Cabrero-Sa&#;udo  Francisco J.  Garc&#;a-Barros  Enrique  Munguira  Miguel L.  Melero  Yolanda 《Landscape Ecology》2022,37(1):81-92

Landscape Ecology - Urbanisation is an environmental filter for many species that leads to community homogenisation, with a few species inhabiting isolated patches (e.g. public and private gardens...  相似文献   

Team assembly mechanisms determine collaboration network structure and team performance   总被引：1，自引：0，他引：1  

Guimerà R  Uzzi B  Spiro J  Amaral LA 《Science (New York, N.Y.)》2005,308(5722):697-702

Agents in creative enterprises are embedded in networks that inspire, support, and evaluate their work. Here, we investigate how the mechanisms by which creative teams self-assemble determine the structure of these collaboration networks. We propose a model for the self-assembly of creative teams that has its basis in three parameters: team size, the fraction of newcomers in new productions, and the tendency of incumbents to repeat previous collaborations. The model suggests that the emergence of a large connected community of practitioners can be described as a phase transition. We find that team assembly mechanisms determine both the structure of the collaboration network and team performance for teams derived from both artistic and scientific fields.  相似文献   

Red foxes (Vulpes vulpes) are a natural intermediate host of Neospora caninum     

Almería S  Ferrer D  Pabón M  Castellà J  Mañas S 《Veterinary parasitology》2002,107(4):287-294

The present study was undertaken to determine if red foxes are natural intermediate and/or definitive host for Neospora caninum and to study the importance of infection of N. caninum in this species in North-eastern Spain. Faecal samples and brain tissues were obtained from 122 foxes from 21 rural areas of Catalonia. Faeces collected were examined for parasite eggs and coccidian oocysts using sucrose flotation. For PCR-based diagnosis of N. caninum in brain tissues, the specific genomic Nc5 region was selected as the target sequence for DNA amplification. To control for PCR failure and facilitate identification of truly negative samples, the competitor pNc5C molecule was added to all negative samples in a second round of PCR reactions. Of the 122 foxes analysed, 13 (10.7%) were positive by PCR for N. caninum. Signal intensities of all positive samples were relatively weak with the exception of one sample from a 3-month male animal, that also showed the highest repeatability. No differences were observed by sex, age or area of sampling analysis. Detection of stages of N. caninum in brain from naturally infected red foxes demonstrated that red foxes are a natural intermediate host for N. caninum. Faecal samples were analysed for the presence of N. caninum oocysts, however, no oocysts compatible with N. caninum were found. A widespread latent infection of red foxes in North-eastern Spain found in the present study indicates that red foxes could have a very important role in the epidemiology of neosporosis in our area.  相似文献