The effect of feeding the leucine metabolite β-hydroxy-β-methylbutyrate (HMB) on cell-mediated immunity and protection against Yersinia ruckeri in pikeperch (Sander lucioperca)     

Andrzej K Siwicki  Zdzis&#;aw Zak&#;&#;  John C Fuller Jr    Steven Nissen  Sylwia Trapkowska  Edward G&#;&#;bski  Krzysztof Kazu&#;  Agata Kowalska  & El&#;bieta Terech-Majewska 《Aquaculture Research》2005,36(1):16-21

The present study examined the influence of leucine metabolite β‐hydroxy‐β‐methylbutyrate (HMB), a natural product HMB, on nonspecific cell‐mediated defence mechanisms and protection against enteric redmouth disease (ERM) in pikeperch (Sander lucioperca). β‐hydroxy‐β‐methylbutyrate was fed in a pelletted ration at 50 mg kg^?1 of the feed for 8 weeks. The phagocytic ability and potential killing activity of blood and pronephric phagocytes were examined in HMB‐ and control‐fed fish before and after 8 weeks of feeding HMB. Simultaneously, the proliferative response of blood and pronephric lymphocytes stimulated by mitogens concanavaline A and lipopolisaccharide were examined in experimental and control groups. Following 8 weeks of HMB feeding, a challenge test was performed by injecting the fish with live pathogenic bacteria Yersinia ruckeri. β‐hydroxy‐β‐methylbutyrate applied in the diet for 8 weeks prompted a statistically significant (P<0.05) increase in phagocytic ability and potential killing activity of the blood and pronephric phagocytes and the proliferative response of blood and pronephric lymphocytes. The changes in these mechanisms correlated with protection against Y. ruckeri, the ERM pathogen. The results showed that feeding HMB increased the nonspecific cell‐mediated defence mechanisms and protection against ERM by reducing cumulative mortality (30%) following the challenge with pathogenic bacteria. Future studies will include determination of optimal doses and protocols of oral application of HMB to maximize the immunomodulatory effects and protection against viral diseases in intensive pikeperch culture.  相似文献   

Assessment of the usefulness of particle size distribution measured by laser diffraction for soil water retention modelling          下载免费PDF全文

Krzysztof Lamorski  Andrzej Bieganowski  Magdalena Ryżak  Agata Sochan  Cezary Sławiński  Wioleta Stelmach 《植物养料与土壤学杂志》2014,177(5):803-813

Particle size distribution (PSD) is a major soil characteristic, which is essential and commonly used for the development of pedotransfer functions (PTFs) to estimate the water retention of soils. The laser diffraction method (LDM) became a popular alternative to the standard sieve‐hydrometer method (SHM) of PSD measurement. Unfortunately, PSDs determined with LDM and SHM methods differ sometimes substantially. Moreover, it is claimed that the laser diffraction method underestimates finer fractions in favor of coarser fractions. Several authors have tried to elaborate on methods to recalculate LDM PSD into its SHM counterparts, but no universal methodology has been developed to this date. In this paper, we use PSD determined by LDM directly for PTF development and compare it with the classical PTF approach based on PSD measured by SHM. Four different PTF models based on LDM particle size distribution data were developed, with different PSD characteristics taken as the models' input variables. The possibility of using alternative PSD characteristics, such as deciles, area moment mean and volume moment mean, for PTF development was examined. The accuracy of PTF models constructed on the basis of LDM‐measured PSD was comparable with that of the developed models using texture data obtained from SHM, giving approximately the same RMSE and R² values. Our study shows that LDM‐measured particle size distribution may be directly used for PTF developments without any recalculations to their sieve‐hydrometer counterparts.  相似文献   

Emerging cultivation of oregano in Sicily: Sensory evaluation of plants and chemical composition of essential oils     

Carmela BonfantiRoberto Iannì  Agata MazzagliaCarmela M. Lanza  Edoardo M. NapoliGiuseppe Ruberto 《Industrial Crops and Products》2012,35(1):160-165

The characterization of the essential oil profile together with the sensory analysis of five samples of oregano, four of which cultivated in different areas of Sicily and one of commercial origin, have been carried out. The Sicilian cultivated samples belong to Origanum vulgare ssp. hirtum (Link) Béguinot (syn. O. heracleoticum L.), the most typical Sicilian oregano species. The essential oils, obtained by hydrodistillation, have been analysed by GC-FID-MS. On the whole, 53 compounds representing more than 98% of the oils have been fully characterized. The four cultivated samples showed a very similar chemical profile, thymol and γ-terpinene being the main compounds followed by p-cymene, α-terpinene, carvacrol and thymol methyl ethers, myrcene and carvacrol. The commercial sample, instead, showed a different profile, with carvacrol largely being the main component (>77%), followed by p-cymene, γ-terpinene and thymol, in that order.According to the chemical composition, the sensory profile of the Sicilian oregano samples proved fairly homogenous, whereas different results were obtained for the commercial sample. Indeed, the intensity of sensory attributes “typical oregano flavour” and off-flavour are similar for the four cultivated samples; instead the commercial sample has less freshness and a lower typical oregano flavour, associated with a higher level of off-flavour.  相似文献   

Dietary resveratrol improves immunity but reduces reproduction of broodstock medaka <Emphasis Type="Italic">Oryzias latipes</Emphasis> (Temminck &amp; Schlegel)     

Agata Kowalska  Andrzej K. Siwicki  Radosław K. Kowalski 《Fish physiology and biochemistry》2017,43(1):27-37

Here, we investigated the effect of dietary resveratrol (20, 40, and 80 µg/g BW/day) on cell-mediated immunity (activity of spleen phagocytes and proliferative response of lymphocytes) and reproductive parameters (egg and sperm quality, i.e. fecundity—total number of eggs produced by individual fish, fertility, embryo survival, and hatching rate) in medaka. Fish fed feed with resveratrol at 40 and 80 µg/g BW/day had significantly higher metabolic activity and intracellular phagocyte killing activity than control. The proliferative lymphocyte activity of the fish from R80 group was greater by more than 20 % in comparison with the control group (P < 0.05). The percentage of macrophages (MO) and their mean fluorescence intensities (MFI) in R40 and R80 groups were significantly higher compared to C and R20 groups (P < 0.05). The differences in MO and MFI values ranged from 52.5 % (±1.5; R0 group) to 65.8 % (±1.6; R80 group) and from 23.2 (±1.4; R0 group) to 38.2 (±2.4; R80 group), respectively. Moreover, resveratrol at 80 µg/g BW/day decreased liver COX activity, i.e. 5.4 in R80 group and 7.9 in R0 group (P < 0.05). The motility parameters of the sperm obtained from the males fed feed supplemented with resveratrol at 80 µg/g BW/day exhibited the highest values except the linearity, which was lower as compared to the control (P < 0.05). The results indicate that diet supplemented with resveratrol at a dosage of 40 µg/g BW/day improves phagocyte killing ability and lymphocyte proliferation in broodstock and accelerates offspring hatch. Also, the results suggest that COX activity influences sperm and oocyte quality in fish; the presence of a COX inhibitor in the dose of 40 µg/g BW/day decreased the embryo survival.  相似文献   

The effect of preservatives on water balance in cut clematis flowers     

Julita Rabiza-Świder  Ewa Skutnik  Agata Jędrzejuk 《The Journal of Horticultural Science and Biotechnology》2017,92(3):270-278

A positive water balance is crucial for longevity of cut shoots. When water loss by transpiration exceeds water uptake by cut flowers, wilting symptoms appear. Clematis is a very attractive plant which can be used as a cut flower provided that suitable cultivars are chosen. The vase life of four cultivars tested ranged between 6.7 days and 9.0 days and the standard preservative – 200 mg·dm^?3 8-hydroxyquinoline citrate (8HQC) plus 2% sucrose – prolonged it significantly in two cultivars, while 8HQC alone was efficient in only one. The preservatives delayed reduction in fresh weight. An enhancement of water uptake by the preservatives resulted in prolonged vase life of flowers in two cultivars. However, the effect of preservatives on transpiration was not related to longevity. In addition, longevity did not depend on the xylem vessel size: the cultivar with the longest vase life had the lowest average xylem vessel diameter while the cultivar with the largest vessels had an intermediate vase life. Preservatives did not always affect the number of blockages in xylem appearing during vase life. Generally, the effects of preservatives on the elements of water balance in cut shoots of clematis were not directly related to the postharvest longevity.  相似文献   

Microstructure of the small intestine in broiler chickens fed a diet with probiotic or synbiotic supplementation     

Joanna Bogucka  David Miguel Ribeiro  Monika Bogus&#x;awska‐Tryk  Agata Dankowiakowska  Rosrio Plcido Roberto da Costa  Marek Bednarczyk 《Journal of animal physiology and animal nutrition》2019,103(6):1785-1791

The aim of the study was to determine the effect of dietary supplementation of a probiotic and a synbiotic on the morphometric parameters of the small intestine of broiler chickens. The experiment was conducted on three hundred sixty, one‐day‐old female Ross 308 chicks, which were randomly selected from 20,000 birds and divided into three treatment groups (n = 120) with ten replicates per treatment. The control group (C) was fed a commercial diet, the probiotic group (PRO) was fed the same diet with an added 1% of the probiotic Lavipan® (Lactococcus lactis, Carnobacterium divergens, Lactobacillus casei, Lactobacillus plantarum and Saccharomyces cerevisiae), and the synbiotic group (SYN) was fed the commercial diet with an added synbiotic: 0.8% of the prebiotic RFO (extracted from lupin seeds) and 1% Lavipan®. According to the manufacturer's data, apart from the typical probiotic action,microorganisms contained in the preparation release anti‐bacterial substances (hydrogen peroxide and bacteriocins) and, therefore, are antagonistic towards pathogenic bacteria present in the gut of animals. Supplementation took place in the first seven days of rearing, and all birds had ad libitum access to water and feed during the 42 days of the experiment. On the last day, all birds were slaughtered and samples from three segments of the small intestine were taken. Villi area, height, width and crypt depth ratios were read using Multiscan software. Synbiotic supplementation increased the BWG of broilers from first to tenth day of rearing, compared to the control group. The PRO group had improved villi morphometric parameters of the duodenum. In the jejunum and ileum, both bioactive substances improved villus width and villus surface area. Crypts were deeper in the small intestine of birds supplemented with bioactive substances, which allows greater renewal of the villi. As expected, the intestinal morphometric parameters of broiler chickens benefited from bioactive substance supplementation.  相似文献   

Chilling stress tolerance of two soya bean cultivars: Phenotypic and molecular responses     

Jakub Kuczyński  Tomasz Twardowski  Jerzy Nawracała  Joanna Gracz-Bernaciak  Agata Tyczewska 《Journal of Agronomy and Crop Science》2020,206(6):759-772

Chilling stress is a major factor limiting the yield of soya bean [Glycine max (L.) Merr.] on a global scale. However, the regulatory network that controls the chilling response of soya bean remains unclear. In the present study, phenotyping and quantitative analyses of miRNAs in soya bean under chilling stress were carried out to determine the impact of environmental constraints on soya bean productivity. Measurements done during soya bean growth in chilling along with the results of field trials indicated that the cultivars Augusta and Fiskeby V responded differently to low temperatures. Although chilling affected the reproductive development of both cultivars, the final seed output remained unchanged. The differential expression of miR169, miR319, miR397 and miR398 under cold stress was detected using ddPCR. Upon chilling in the reproductive stage, we found that these miRNAs had contrasting expression profiles in Augusta and Fiskeby V. A set of candidate target genes was predicted based on degradome sequencing data. A negative correlation was found between the expression of miR169, miR319 and miR398 and their targets in the roots of both cultivars. Our work elucidates the impact of chilling stress on the productivity of two soya bean cultivars and reveals the importance of miRNA involvement in the low temperature response.  相似文献   

Phenolics of Arbutus unedo L. (Ericaceae) fruits: identification of anthocyanins and gallic acid derivatives     

Pawlowska AM  De Leo M  Braca A 《Journal of agricultural and food chemistry》2006,54(26):10234-10238

Arbutus unedo L., the strawberry tree (Ericaceae family), is an evergreen shrub or small tree, typical of the Mediterranean fringe and climate. The aim of the present study was to evaluate the profile of the phenolic constituents of A. unedo fruits. Seven compounds were purified by Sephadex LH-20 column chromatography of the MeOH extract followed by HPLC and were characterized as arbutin, beta-D-glucogalline, gallic acid 4-O-beta-D-glucopyranoside, 3-O-galloylquinic acid, 5-O-galloylquinic acid, 3-O-galloylshikimic acid, and 5-O-galloylshikimic acid, by means of NMR and ESI-MS analyses. Moreover, LC-PDA-MS analysis of the red pigment of A. unedo fruits revealed the presence of three anthocyanins recognized as cyanidin 3-O-beta-D-galactopyranoside, delphinidin 3-O-beta-D-glucopyranoside, and cyanidin 3-O-beta-D-arabinopyranoside. These pigments were also quantified.  相似文献   

Diversity of Peptides Produced by Nodularia spumigena from Various Geographical Regions     

Hanna Mazur-Marzec  Monika J. Kaczkowska  Agata Blaszczyk  Reyhan Akcaalan  Lisa Spoof  Jussi Meriluoto 《Marine drugs》2013,11(1):1-19

Cyanobacteria produce a great variety of non-ribosomal peptides. Among these compounds, both acute toxins and potential drug candidates have been reported. The profile of the peptides, as a stable and specific feature of an individual strain, can be used to discriminate cyanobacteria at sub-population levels. In our work, liquid chromatography-tandem mass spectrometry was used to elucidate the structures of non-ribosomal peptides produced by Nodularia spumigena from the Baltic Sea, the coastal waters of southern Australia and Lake Iznik in Turkey. In addition to known structures, 9 new congeners of spumigins, 4 aeruginosins and 12 anabaenopeptins (nodulapeptins) were identified. The production of aeruginosins by N. spumigena was revealed in this work for the first time. The isolates from the Baltic Sea appeared to be the richest source of the peptides; they also showed a higher diversity in peptide profiles. The Australian strains were characterized by similar peptide patterns, but distinct from those represented by the Baltic and Lake Iznik isolates. The results obtained with the application of the peptidomic approach were consistent with the published data on the genetic diversity of the Baltic and Australian populations.  相似文献   

Wet meadow restoration in Western Europe: A quantitative assessment of the effectiveness of several techniques     

Agata Klimkowska  Rudy Van Diggelen  Jan P. Bakker  Ab P. Grootjans   《Biological conservation》2007,140(3-4):318-328

Techniques such as rewetting, topsoil removal, diaspore transfer or combinations of these are increasingly applied in fen meadow and flood meadow restoration in Western Europe. In this paper, we present a quantitative assessment of the effectiveness of the commonly used meadow restoration methods. We use the change in ‘saturation index’ to evaluate the degree of success. The index reflects the completeness of restored communities in comparison to regional target communities. Meadow restoration has limited success in most cases, with an average increase in species richness below 10% of the regional species pool. Restoration success was partly determinated by the starting situation. The more species-rich the starting situation, the higher the saturation index after restoration but, at the same time, the smaller the increase in the number of target species due to restoration. Top soil removal and diaspore transfer were found to contribute most to restoration success. A combination of top soil removal and diaspore transfer and a combination of all three techniques appeared to be the most effective measure and resulted in an increase in the saturation index of up to 16%. Rewetting alone had no measurable effect on restoration success.  相似文献