Failure to detect transmission of Escherichia coli O157:H7 from orally dosed nannies to sucking kids at foot     

La Ragione RM  Ahmed NM  Best A  Clifford D  Weyer U  Woodward MJ 《The Veterinary record》2005,157(21):659-661

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Effects of oxygen tension in the gas atmosphere during in vitro maturation, in vitro fertilization and in vitro culture on the efficiency of in vitro production of mouse embryos   总被引：2，自引：0，他引：2  

Adam AA  Takahashi Y  Katagiri S  Nagano M 《The Japanese journal of veterinary research》2004,52(2):77-84

Effects of oxygen (O2) tension in the gas atmosphere during in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro culture (IVC) on the efficiency of in vitro production of mouse embryos were examined. Mouse oocytes recovered from large antral follicles were subjected to IVM in Waymouth medium for 15, 16 and 17 hr under 5 or 20% O2 and then subjected to IVF and IVC under 5 or 20% O2 tension. Lowering the O2 tension in the gas atmosphere for IVM from 20 to 5% improved the cleavage rate after IVF when the oocytes were subjected to IVM for 15 hr; however, no improvement in the cleavage rate was observed when the culture period for IVM was extended to 16 and 17 hr. Lowering the O2 tension to 5% for IVM and IVC improved the development of the cleaved oocytes to the blastocyst stage, regardless of the culture period for IVM. However, the O2 tension for IVF had no remarkable effect on the subsequent embryonic development. These results demonstrate that 5% O2 is superior to 20% O2 for IVM and IVC, and suggest that 20% O2 for IVM may delay oocyte maturation and/or the acquisition of fertilizability and impair the developmental competence of oocytes.  相似文献   

Fixation to the canine bone of artificial implant with new surface structure     

Seno T  Izumisawa Y  Nishimura I  Maehara S  Kushiro T  Umar MA  Wakaiki S  Yamashita K  Kotani T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(3):315-318

Screw and laser (SL) column by making screw threads and forming small holes using laser irradiation on the base metal and conventional beads coating (BC) columns were embedded into the shaft of canine femurs, and compared the implant fixation to the host bone. The interfacial strength in SL columns was almost equivalent as BC columns, and bone-column contact rate was higher than BC columns significantly at twelve weeks after implantation. The newly devised SL surface had almost equivalent bone fixation strength comparable to the conventional BC surface. Also, this surface should provide a useful porous surface for use in artificial joints since there is no risk of surface structure detachment.  相似文献   

Bovine hepatocyte growth factor and its receptor c-Met: cDNA cloning and expression analysis in the mammary gland     

Yamaji D  Kimura K  Watanabe A  Kon Y  Iwanaga T  Soliman MM  Ahmed MM  Saito M 《Domestic animal endocrinology》2006,30(3):239-246

Hepatocyte growth factor/scatter factor (HGF/SF) is a pleiotropic cytokine that plays a crucial role in the embryonic and postnatal development of various organs including the mammary gland. We cloned bovine HGF and its c-Met receptor cDNAs, and examined their expression during mammary gland development in dairy cows. The 2.5-kbp HGF cDNA clone contained a 2190 bp open reading frame coding a 730 amino acid protein, while the 4.8-kbp c-Met cDNA clone contained a 4152 bp open reading frame coding a 1384 amino acid protein. The bovine HGF and c-Met sequences exhibited more than 87% identity with those of other mammals. RT-PCR analysis revealed ubiquitous expression of both HGF and c-Met mRNAs in various bovine tissues tested. HGF mRNA was detected only in the inactive stage of bovine mammary gland development and not in the developing, lactating, and involuting stages, while c-Met mRNA was detected in the inactive and involuting stages. Immunohistochemical analysis demonstrated that the c-Met protein was found on mammary epithelial cells in the inactive, developing, and involuting stages, and on myoepithelial cells in all stages. These results suggest pivotal roles of HGF and c-Met in the development of bovine mammary gland.  相似文献   

Introduction     

Ahmed J 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2006,53(Z1):1

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Unusual manifestation of a concurrent demodectic and sarcoptic mange in a Zebu-Friesian cross-bred heifer     

Hamid ME  Alla KM  Ahmed SS  El Shiekh AE  Ibrahim KE 《Journal of the South African Veterinary Association》2006,77(2):90-91

We describe a rare case of a concurrent demodectic and sarcoptic mange in a 2-year-old heifer in Khartoum, Sudan. The lesions were massive lumps of granulomatous tumour-like dermatitis with thick, nodular folds mainly covering the head, neck and shoulders. Histopathological examination of the lesions revealed the presence of both Demodex bovis and Sarcoptes scabiei. The animal died regardless of the anti-parasitic treatment it received.  相似文献   

Loss of virulence of the phytopathogen Ralstonia solanacearum through infection by φRSM filamentous phages     

Addy HS  Askora A  Kawasaki T  Fujie M  Yamada T 《Phytopathology》2012,102(5):469-477

φRSM1 and φRSM3 (φRSM phages) are filamentous phages (inoviruses) that infect Ralstonia solanacearum, the causative agent of bacterial wilt. Infection by φRSM phages causes several cultural and physiological changes to host cells, especially loss of virulence. In this study, we characterized changes related to the virulence in φRSM3-infected cells, including (i) reduced twitching motility and reduced amounts of type IV pili (Tfp), (ii) lower levels of β-1,4-endoglucanase (Egl) activity and extracellular polysaccharides (EPS) production, and (iii) reduced expression of certain genes (egl, pehC, phcA, phcB, pilT, and hrpB). The significantly lower levels of phcA and phcB expression in φRSM3-infected cells suggested that functional PhcA was insufficient to activate many virulence genes. Tomato plants injected with φRSM3-infected cells of different R. solanacearum strains did not show wilting symptoms. The virulence and virulence factors were restored when φRSM3-encoded orf15, the gene for a putative repressor-like protein, was disrupted. Expression levels of phcA as well as other virulence-related genes in φRSM3-ΔORF15-infected cells were comparable with those in wild-type cells, suggesting that orf15 of φRSM3 may repress phcA and, consequently, result in loss of virulence.  相似文献   

Effect of antioxidant supplementation on free radical scavenging system and immune response in lindane treated scabies patients     

Mythily SubramaneyaanSachin Rustagi  Sambit N. BhattacharyaAsok K. Tripathi  Basu D. BanerjeeRafat S. Ahmed 《Pesticide biochemistry and physiology》2012,102(1):91-94

The scabicide, lindane induces oxidative stress and immunological alterations. The present study was undertaken to assess the ameliorative effects of antioxidant supplementation in lindane treated scabies patients. Scabies patients were treated with either 1% lindane or 1% lindane along with antioxidant (Lycored or Vitamin-E). Oxidative stress and immunological parameters were evaluated in blood samples and compared with healthy controls. Lindane caused a significant increase in malonedialdehyde (MDA) levels and decrease in reduced glutathione (GSH) and ferric reducing ability of plasma (FRAP), which was attenuated by anti-oxidant therapy. The IL-1α levels were significantly enhanced in scabies patients per se and remained unaffected after lindane/anti-oxidant treatment. The TNF-α and nitroblue tetrazolium (NBT) reduction levels were not significantly different in all the groups. Topical application of lindane induces significant free radical generation and may cause immunological alterations which can be reversed by antioxidant therapy.  相似文献   

Assessment of the impact of resistant and susceptible canola on Plasmodiophora brassicae inoculum potential     

S. F. Hwang  H. U. Ahmed  Q. Zhou  S. E. Strelkov  B. D. Gossen  G. Peng  G. D. Turnbull 《Plant pathology》2012,61(5):945-952

The impact on clubroot severity of growing susceptible canola or mixtures of resistant and susceptible canola genotypes was examined. Bioassays revealed greater clubroot severity and incidence, and reduced plant height, where 100% of a susceptible cultivar had been grown. A higher proportion of susceptible plants within a resistant canola crop increased root hair and secondary infections. Regression analysis of root hair infection and the amount of Plasmodiophora brassicae DNA (as determined by quantitative PCR) revealed strong linear relationships between the two parameters. The linear relationships between root hair infection and P. brassicae DNA were stronger for the resistant cultivar than for the susceptible cultivar when regression analysis was conducted by cultivar over the sampling dates. In conclusion, the cropping of a resistant cultivar reduced clubroot severity, while the presence of susceptible volunteer canola increased inoculum potential. Quantitative PCR was a reliable tool for the quantification of root hair infection.  相似文献   

中国扶贫瞄准机制的创新与实践——以广东省连南县为例     

于敏  张晓颖  Salehuddin Ahmed 《农业现代化研究》2012,33(2):199-202

本文以广东省连南县为例,介绍了"规划到户、责任到人"(简称"双到")的扶贫瞄准机制,即根据贫困户的发展意愿和特点,制定发展规划,明确贫困户对应的帮扶责任人和考评标准。通过此项目的实施,广东省有效地提高了贫困户收入,降低了贫困发生率和多维贫困;提高了农民自我可持续发展能力,活跃了农村经济。"双到"扶贫项目是在经济较发达地区缩小贫富差距的一个案例,为实现低保和扶贫的衔接、城乡资源双向流动、动态贫困瞄准、扶贫任务的"官员问责制"提供了借鉴。  相似文献