Genetic Polymorphisms in LTF/EcoRI and TLR4/AluI loci as candidates for milk and reproductive performance assessment in Holstein cattle     

Wael B. El‐Domany  Hend A. Radwan  Ahmed I. Ateya  Hazem H. Ramadan  Basma H. Marghani  Sherif M. Nasr 《Reproduction in domestic animals》2019,54(4):678-686

The aim of this study was to explore the genetic polymorphisms in LTF/EcoRI and TLR4/AluI loci and their association with milk and reproductive performance in Holstein cattle. A randomly selected 800 Holstein dairy cows from two dairy farms (400 animals each) in Egypt were used. Based on the two farm records, association between LTF/EcoRI genotypes and milk performance traits (order of lactation, daily milk yield, days in milk, corrected milk at 305 day and dry period) was carried out. Meanwhile, exploring of TLR4/AluI genotypes effect was done on data for reproductive performance (age at first freshening, calving interval, number of services per conception, ovarian rebound and days open). DNA was extracted from blood samples collected from Holstein dairy cows of the both farms and restriction analysis of 301‐bp PCR products of LTF gene revealed two genotypes: AA genotype (301 bp) and AB genotype (301, 201 and 100 bp). Meanwhile, restriction analysis of 382‐bp PCR products of TLR4 gene digested with AluI yielded two alleles (A and B) and three genotypes (AA, AB and BB). The A allele was indicated by two bands at 300 and 82 bp, and the B allele resulted in three fragments of 160, 140 and 82 bp. There was a significant association (p ≤ 0.05) between LTF genotypes and milk performance traits except for days in milk. The TLR4 genotypes had significant effects (p ≤ 0.05) on age at first freshening, calving interval, number of services per conception, ovarian rebound and days open. Ordinal logistic regression statistical model also revealed that it is possible to calculate high reproductive performance traits and to predict favourable dairy cows based on LTF and TLR4 genotypes. This research reveals the effectiveness of LTF/EcoRI and TLR4/AluI loci as candidates for reproductive performance assessment in Holstein cattle.  相似文献   

Leptin inhibits mitogen-induced proliferation of peripheral T lymphocytes from Holstein cows     

Ahmed M  Kimura K  Soliman M  Yamaji D  Okamatsu-Ogura Y  Ishioka K  Makondo K  Hagiwara K  Saito M 《Veterinary journal (London, England : 1997)》2008,176(3):361-368

This study examined the mitogenic response of bovine peripheral T lymphocytes to leptin, a pleiotropic hormone regulating food intake and energy expenditure. Leptin alone slightly suppressed proliferation of T lymphocytes in the presence of concanavalin A (ConA). Leptin also inhibited proliferation of T lymphocytes induced by anti-CD3 antibody. ConA treatment activated some protein kinases, including p44/p42(MAPK) and Akt/PKB, while anti-CD3 antibody treatment increased mRNA expression of suppressor of cytokine signalling (SOCS) 3, interferon (IFN)gamma, interleukin (IL) 2 and IL4 in T lymphocytes. Leptin alone increased only SOCS3 mRNA expression. Simultaneous treatment with mitogens and leptin enhanced IFNgamma mRNA expression but decreased IL2 mRNA expression, without any synergistic effect on phosphorylation of protein kinases or mRNA expression of SOCS3 and IL4. These results suggest that leptin modulates bovine T lymphocyte functions.  相似文献   

Performance of New Zealand White and Californian male weaned rabbits in the subtropical environment of Egypt     

Ibrahim F. M. MARAI  Al-Sa-ied A. M. HABEEB  Ahmed E. GAD 《Animal Science Journal》2008,79(4):472-480

The New Zealand White (NZW) was superior significantly ( P  < 0.05) to the Californian (Cal) breed in final body weight, final total body solids and preslaughter weight. In contrast, Cal showed greater plasma creatinine ( P  < 0.001) than the NZW. The differences in the other traits were not significant. NZW also showed higher dressing percentage and Cal showed higher mortality percentage. The Temperature-humidity Index (THI) values indicated absence of heat stress in the first period (mild period) and exposure to moderate (very close to severe) heat stress in the second one (hot period). The heat stress conditions caused significant ( P  < 0.001, 0.01 or 0.05) decreases in each of final body weight, daily body gain, daily feed intake, final total body solids, solids daily gain, plasma total protein, albumin, globulin, total lipids, glucose, thyroid hormone T3, sodium, potassium, calcium, magnesium and phosphorus and preslaughter weight. At the same time, significant ( P  < 0.001 or 0.01) increases occurred in each of water intake, water/feed ratio, total body water (mL/100 g body solids), blood urea-N, creatinine, respiration rate and rectal, skin and ear temperatures. The interaction of breed x period effects was significant ( P  < 0.01 or 0.05) on plasma creatinine and hind-limb cut. Estimations of 'adaptability' to the subtropical environment of Egypt were found to be 83.1% for NZW and 82.6% for Cal breeds.  相似文献   

Age‐related histomorphometric and immunohistochemical changes of the moderator band in Egyptian Baladi cattle     

Mahmoud Abdelghaffar Emam  Ahmed I. Abo‐Ahmed 《Anatomia, histologia, embryologia》2019,48(2):149-156

The moderator band (MB) is a common fibromuscular anatomical structure for the right ventricle of most animals. The histomorphometric and immunohistochemical analysis of the MBs of Egyptian Baladi cattle in relation to age was the aim of this study. Eighteen clinically healthy animals of both sexes were used for this study. The animals were divided into three groups depending on age, group I (N = 4, <1 year), group II (N = 8, 1–2 years) and group III (N = 6, 4–8 years). Cross sections of the MBs from all groups were stained with H&E, Masson's trichrome and anti‐connexin43 (Cx43) antibody for histological and immunohistochemical examinations. Also, measurements for the thickness of the endocardium of the MB as well as, the wall of its muscular artery were conducted. Bundles of Purkinje fibres (PFs) were identified peripherally in the endocardial layer and among the myocardial fibres in the core of each MB. The infiltration of endocardial adipocytes was the characteristic for MBs of old animals. All morphometric data showed a significant increase with the advancement of age. Immunohistochemical findings revealed the localization and distribution of Cx43 in the PFs and intercalated discs of all examined MBs. However, variation of Cx43 immunoreactivity was found among the groups depending on the age. On the basis of this study, this conclusion of different histomorphometry and Cx43 expression of the MBs in relation to age was drawn. These interesting findings provide further insight into age‐related physiological and pathological heart conditions.  相似文献   

Intestinal gene expressions in broiler chickens infected with Escherichia coli and dietary supplemented with probiotic,acidifier and synbiotic     

Ateya  Ahmed I.  Arafat  Nagah  Saleh  Rasha M.  Ghanem  Hanaa M.  Naguib  Doaa  Radwan  Hend A.  Elseady  Y. Y. 《Veterinary research communications》2019,43(2):131-142

In this study, we investigated the effects of probiotic, acidifier and synbiotic supplementation on growth performance, mortality rate, intestinal gene expressions, fecal shedding, and organs colonization induced by Escherichia coli in broiler chickens. Six experimental groups were included; negative control group (NC), positive control group (PC), probiotic group (PR), acidifier group (AC), synbiotic group (SY) and colistin sulfate group (CS). Chickens in groups NC and PC were fed a basal diet, while chickens in groups PR, AC, SY, and CS were fed a basal diet containing probiotic, acidifier, synbiotic and colistin sulfate, respectively from the 1st day to the 28th day of age. At 7 days of age, all groups (not NC) were orally challenged with 0.5 ml (1.0?×?10⁹ CFU/ml) E. coli O78. The dietary supplementation of acidifier and synbiotic were sufficient to quell the devastating effects of E. coli infection in broilers. Growth performances represented by body weight gain, feed intake and feed conversion ratio were significantly improved as well as, mortalities were prevented whilst the ileal pro-inflammatory gene expressions (IL-6, IL-8, IL-13, TLR-4, IFN-γ, LITAF, AvBD-2, and AvBD-9) were significantly downregulated and the anti-inflammatory cytokine (IL-10) was significantly increased. In addition, E. coli fecal shedding and organs colonization was significantly diminished. It was concluded that the addition of both acidifier and synbiotic to the diet of broilers infected with E. coli could modulate the intestinal inflammatory responses induced by E. coli infection and minimized the inflammation-induced damage which resulted in improvement in growth performance, prevention of mortalities and reduction of E. coli environmental contamination.

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浙江金华地区PCV2分子流行病学调查及遗传变异分析     

吴明臻  江云波  敖超杰  于学祥  库旭钢  陈芳洲  孙琪  吴昊  何启盖  赵青 《中国兽医学报》2019,(10):1890-1896

猪圆环病毒2型(porcine circovirus type 2,PCV2)主要引起猪的多系统功能障碍性疾病,同时,还可侵害猪的免疫系统,造成猪免疫抑制,容易继发细菌及其他病毒感染,大大增加猪群的死亡率。为了解PCV2在浙江金华猪群中的分布与流行状况以及PCV2变异规律,对从浙江省金华猪场收集的3 433份血清进行PCR检测,结果显示,2012-2018年PCV2阳性率分别为10.16%,11.95%,8.50%,9.94%,6.19%,5.94%,8.28%,母猪阳性率最高为13.49%。本研究发现在2012年浙江金华地区PCV2优势毒株已经由PCV2b变为PCV2d,且全部为PCV2d-2。将得到的ORF2序列同已知的疫苗序列对比后,发现SH株与浙江金华目前PCV2主流毒株序列一致性最高,但抗原位点比较仍有5处差异,这可能会对疫苗效果产生影响,需要进一步验证。另外只有PCV2d在免疫显性诱饵表位上发生了突变,这可能是PCV2d成为主流的原因。  相似文献   

Skeletal muscle and liver gene expression profiles in finishing steers supplemented with Amaize          下载免费PDF全文

Ahmed A. Elolimy  Sonia J. Moisá  Kristen M. Brennan  Allison C. Smith  Daniel Graugnard  Daniel W. Shike  Juan J. Loor 《Animal Science Journal》2018,89(8):1107-1119

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Risk analysis and seroprevalence of bovine ephemeral fever virus in cattle in the Kingdom of Saudi Arabia     

Ahmed Zaghawa  Fadhel Mohamed Taher Housawi  Abdulmohsen Al-Naeem  Hassan Al-Nakhly  Ahmed Kamr  Ramiro Toribio 《Tropical animal health and production》2016,48(3):487-492

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Fertility,mortality, milk output,and body thermoregulation of growing Hy-Plus rabbits fed on diets supplemented with multi-enzymes preparation     

Hany M. Gado  Ahmed E. Kholif  Abdelfattah Z. M. Salem  Mona M. M. Elghandour  Oluwarotimi A. Olafadehan  Maricela A. Martinez  Ahmed Q. Al-Momani 《Tropical animal health and production》2016,48(7):1375-1380

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Pathological and biochemical studies on experimental hypothyroidism in growing lambs     

Sokkar SM  Soror AH  Ahmed YF  Ezzo OH  Hamouda MA 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2000,47(9):641-652

Secondary iodine deficiency was experimentally produced in growing male lambs by oral administration of 50 mg/kg bodyweight of Thiourea daily for 3.5 months. At the end of the experiment the animals became weak, emaciated, anaemic, significantly reduced in body weight with facial oedema and alopecia at thigh, legs and abdomen. The clinical analysis showed significant reduction in erythrocyte and leucocyte numbers and in levels of triiodothyronine and testosterone at the end of the experiment. The histopathological picture of the thyroid gland revealed hyperplasia of the follicle-lining epithelial cells which project into the lumen. The lumens of the follicles are devoid of colloid. The testes showed ill-developed small, empty seminiferous tubulcs. In the liver, the hepatocytes showed degeneration and vacuolation with proliferation of Kupffer cells, which contain haemosiderin pigment. The kidney showed glomerular lipidosis with accumulation of haemosiderin pigment in the cytoplasm of the renal tubules. Hyperkeratosis of the epidermis associated with excessive keratin formation within the hair follicles was detected. In conclusion, deficiency of iodine causes hypothyroidism which leads to retardation of growth, reduced wool production and interferes with sexual maturity of growing male lambs.  相似文献