Field comparison of the interferon-gamma assay and the intradermal tuberculin test for the diagnosis of bovine tuberculosis   总被引：13，自引：0，他引：13  

PR WOOD  LA CORNER  JS ROTHEL  C. BALDOCK†  SL JONES†  DB COUSINS§  BS McCORMICK¶  BR FRANCIS§  J. CREEPER¶  NE TWEDDLE 《Australian veterinary journal》1991,68(9):286-290

An extensive field comparison of the gamma interferon (IFN-gamma) assay and the single intradermal tuberculin test for the diagnosis of bovine tuberculosis was conducted in Australia. The specificity of the IFN-gamma assay was determined by testing more than 6000 cattle from tuberculosis-free herds and varied from 96.2% to 98.1%, depending on the cut-off point chosen to define a positive reactor. For the sensitivity trial, cattle from herds being de-populated because of bovine tuberculosis were examined with both assays. The sensitivity of the IFN-gamma assay was shown to be significantly higher than the single intradermal tuberculin test and varied from 76.8% to 93.6% depending on the method of interpretation. A maximum overall sensitivity of 95.2% was obtained by testing with the IFN-gamma and the tuberculin test in parallel. The superior sensitivity of the IFN-gamma assay and the ability to adjust the sensitivity of the system depending on the task involved, will provide the Australian Tuberculosis Eradication Campaign with a valuable additional test to enable it to accomplish its goals.  相似文献   

Transdifferentiation of porcine satellite cells to adipoblasts with ciglitizone     

Singh NK  Chae HS  Hwang IH  Yoo YM  Ahn CN  Lee SH  Lee HJ  Park HJ  Chung HY 《Journal of animal science》2007,85(5):1126-1135

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Serological evidence of Neospora caninum in alpacas from eastern Australia          下载免费PDF全文

JS King  JL Vaughan  PA Windsor 《Australian veterinary journal》2015,93(7):259-261

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Isolation of Nocardia mexicana from focal proliferative tenosynovitis and arthritis in a steer          下载免费PDF全文

H Owen  K Buckle  J Olm  M Leitner  S Pandey  JB Gaughan  ML Sullivan  AM Lees  JS Gibson 《Australian veterinary journal》2015,93(5):170-173

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How do heavy metal ATPases contribute to hyperaccumulation?          下载免费PDF全文

Won Park  Sung‐Ju Ahn 《植物养料与土壤学杂志》2014,177(2):121-127

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Wnt5a control of cell polarity and directional movement by polarized redistribution of adhesion receptors     

Witze ES  Litman ES  Argast GM  Moon RT  Ahn NG 《Science (New York, N.Y.)》2008,320(5874):365-369

Mechanisms by which Wnt pathways integrate the organization of receptors, organelles, and cytoskeletal proteins to confer cell polarity and directional cell movement are incompletely understood. We show that acute responses to Wnt5a involve recruitment of actin, myosin IIB, Frizzled 3, and melanoma cell adhesion molecule into an intracellular structure in a melanoma cell line. In the presence of a chemokine gradient, this Wnt-mediated receptor-actin-myosin polarity (W-RAMP) structure accumulates asymmetrically at the cell periphery, where it triggers membrane contractility and nuclear movement in the direction of membrane retraction. The process requires endosome trafficking, is associated with multivesicular bodies, and is regulated by Wnt5a through the small guanosine triphosphatases Rab4 and RhoB. Thus, cell-autonomous mechanisms allow Wnt5a to control cell orientation, polarity, and directional movement in response to positional cues from chemokine gradients.  相似文献   

GC-MS analysis of curcumin dye after selective degradation treatment     

Cheunsoon Ahn  S. Kay Obendorf 《Fibers and Polymers》2007,8(3):278-283

Curcumin dye in methanol solution was treated in 100°C oven (OV) and H₂O₂/UV/O₂ (PER) conditions and the degradation products were examined using gas chromatography mass spectrometry (GC-MS). Curcumin itself was not detected from either the OV or PER degraded samples. From OV degradation samples curcumene1, o-coumaric acid2, vanillin3, 2,4-di-tert-butylphenol4, benzene, 1-(3-cyclopentylpropyl)-2,4-dimethyl-45, 1-(4-hydroxy-benzylidene) acetone6, feruloylmethane7, and 2-propenoic acid, 3-[4-(acetyloxy)-3-methoxy phenyl]-, methyl ester8 were detected. From PER degraded samples new products benzoic acid9 and vanillic acid10 were detected additionally. Under the same GC-MS instrumental condition, it is possible that the burial degraded curcumin dye or curcumin dyed textile will exhibit area peaks around 6.2∼6.5 min and 8.3∼8.4 min retention times, which have the mass spectrum matching closely with products3, 4, 7, 8 or9, 10.  相似文献   

Effect of enzyme treatment and wood pulp variation on physical characteristics and fabric hand of lyocell fabrics     

Cheunsoon Ahn  Hye-Ja Yoo  Hye Ja Lee  Jung Hee Kim  Kyung-Hun Song  Jeon Sook Rhie 《Fibers and Polymers》2005,6(1):28-34

The purpose of the research was to examine the effect of three different levels of enzyme treatment on the physical characteristics and the end-use suitability of the lyocell fabrics made with four different types of commercial wood pulp. The appropriate enzyme concentration for obtaining an optimum as well as consistent physical characteristics and fabric hand trait was 3 g/l for the concentration levels tested in the present investigation. Weight loss was more affected by higher enzyme concentration than other physical properties. H2 was least affected by enzyme treatment for all three physical properties and fabric hand. 5 g/l concentration exhibited little difference from 3 g/l in the physical characteristics, whereas the KES-FB values indicated a significant loss of fabric hand in most PHVs with the 5 g/l concentration level. Among different garment parameters, all four fabric types were relatively inappropriate for the men’s slacks (MS) fabric due to the lower hand value of koshi required by the MS parameter. However, despite the relatively low koshi values, high fukurami values required for men’s dress shirt (MWDS) resulted in the highest THV among the four garment parmeters. The four fabric types, which represent the usage of four different wood pulps, in general seem to exhibit a higher applicability to women’s winter thin dress (WWTD) than women’s winter suit (WWS) garment parameter.  相似文献   

Co-occurrence of autoimmune thyroid disease in a multiple sclerosis cohort     

JS Sloka  Pryse-WEM Phillips  M Stefanelli  C Joyce 《Journal of Autoimmune Diseases》2005,2(1):9

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Up‐Regulation of Insulin‐Like Growth Factor I and Uteroglobin in In Vivo‐Developed Parthenogenetic Embryos     

C Naturil‐Alfonso  JS Vicente  DS Peñaranda  F Marco‐Jiménez 《Reproduction in domestic animals》2013,48(1):126-130

Parthenote embryos are being considered as an alternative source of embryonic stem cells. However, as there is still a dearth of knowledge of this kind of embryos, a better understanding of their biology is needed for their application. In this work, we studied the differences and similarities between parthenotes and normal embryos at the blastocyst stage in vivo developed. We analysed the expression of factor OCT‐4, vascular endothelial growth factor (VEGF), insulin‐like growth factor I (IGF‐I) and uteroglobin (UG) by real‐time PCR. To do so, oocytes were recovered and after activation procedure were transferred by ventral middle laparoscopy to receptive does to undergo completely in vivo development. Does were slaughtered 6 days post‐ovulation induction, and parthenote and normal embryos were recovered for mRNA expression analysis. Our results reported that parthenotes and normal embryos showed similar mRNA expression for OCT‐4 and VEGF. However, IGF‐I and UG showed to be over‐expressed in parthenote embryos. Thus, our study highlights that despite the in vivo development of parthenotes, they still seem to have an altered expression and, therefore, to be different to normal embryos. The altered expression pattern of parthenote embryos suggests that these embryos should be studied carefully before future application.  相似文献