Production of Ecteinascidia turbinata (Ascidiacea: Perophoridae) for Obtaining Anticancer Compounds     

José Luis  Carballo Santiago  Naranjo  Bulent  Kukurtzü  Fernando  de La Calle Aida  Hernández-Zanuy 《Journal of the World Aquaculture Society》2000,31(4):481-490

Two methods have been tested in order to produce large quantities of Ecteinascidia turbinata for the extraction of anti-tumoral substances. With the first method, the capacity of generating biomass from settlement of larvae in a natural environment was estimated. The second method consisted of fragmenting colonies into small pieces, which were transplanted onto plastic screen where partial periodic collections of the colony could be carried out. For the first method, two different artificial substrates for settlement of larvae were studied: lines composed of hanging strips of wood, and plastic cords. The average production per unit of surface was significantly different between the two artificial substrates ( P < 0.005) (4.25 kg/M² on the wooden strips after 75 days versus 1.39 kg/m² on the plastic cords after 80 d). The transplant experiment showed a 93% survival of the colony 26 d after the colonies were transplanted and settled, and 96.48 kg of average biomass were collected, indicating a 235% increase in biomass. This system permits stolonial regrowth of the biomass fixed on the surface, without new transplants or settlements being needed, so that a continuous production can be maintained. The fragmentation of the colonies stimulate genet growth rates through the production of small daughter colonies which individually have higher relative growth rates than large colonies and, therefore, collectively have higher absolute growth rates than a large colony of similar biomass. The production of E. turbinata using a combination of the two methods is an example of successful production of metabolites from marine organisms while protecting natural populations.  相似文献   

Time-course response of plasma testosterone and estradiol-17β to salmon gonadotropin treatment in the Japanese eel during ovarian development     

Narumi Sato  Ichiro Kawazoe  Yuzuru Suzuki and  Katsumi Aida 《Fisheries Science》2000,66(4):792-794

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Changes in Histidine and Anserine Levels in Hatchery-Reared Honmasu Salmon Parr after Release in a River   总被引：1，自引：0，他引：1  

Arimune  Munakata Katsumi  Aida Masafumi  Amano Kazumasa  Ikuta Shoji  Kitamura Hiroshi Y.  Ogata 《Journal of the World Aquaculture Society》2000,31(2):274-278

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Changes in heart rate and heart rate variability in Thoroughbreds during prolonged road transportation     

Ohmura H  Hiraga A  Aida H  Kuwahara M  Tsubone H  Jones JH 《American journal of veterinary research》2006,67(3):455-462

OBJECTIVE: To determine whether evaluation of heart rate (HR) and HR variability (HRV) during prolonged road transportation in horses provides a sensitive index of autonomic stimulation. ANIMALS: Five 2-year-old Thoroughbreds. PROCEDURE: ECGs were recorded as horses were transported for 21 hours in a 9-horse van. Heart rate, high-frequency (HF) power, low-frequency (LF) power, and LF-to-HF ratio from Fourier spectral analyses of ECGs were calculated and compared with values recorded during a 24-hour period of stall rest preceding transportation. RESULTS: HR, HF power, and LF power had diurnal rhythms during stall rest but not during road transportation. Heart rate was higher and HF power and LF power lower during road transportation than stall rest, and HR, HF power, LF power, and LF-to-HF ratio all decreased with time during road transportation. Heart rate during stall rest was weakly and inversely associated with LF power, but during road transportation was strongly associated with LF power, HF power, and LF-to-HF ratio. Neither LF power nor HF power was correlated with LF-to-HF ratio during stall rest, but LF power was strongly and HF power weakly correlated with LF-to-HF ratio during road transportation. High-frequency power and LF power were significantly correlated with each other during stall rest and road transportation. Heart rate was significantly influenced by LF power and LF-to-HF ratio during stall rest (R(2) = 0.40) and by HF power and LF-to-HF ratio during road transportation (R(2) = 0.86). CONCLUSIONS AND CLINICAL RELEVANCE: HR is influenced by different sympathovagal mechanisms during stall rest, compared with during road transportation; HRV may be a sensitive indicator of stress in transported horses.  相似文献   

Influence of olive maturity stage and geographical origin on some minor components in virgin olive oil of the chemlali variety     

Lazzez A  Perri E  Caravita MA  Khlif M  Cossentini M 《Journal of agricultural and food chemistry》2008,56(3):982-988

Minor compounds such as sterols, aliphatic alcohols, tocopherols, and chlorophylls in virgin olive oil from Chemlali cultivar were analyzed during the maturity process. The study concerns oils from the following three different olive growing areas of Tunisia: Sfax, Sidi Bouzid, and Enfidha. Analytical results showed that both the maturity process and the olive provenances influence the evolution of the content of these compounds.  相似文献   

Association between preoperative characteristics and risk of anaesthesia-related death in dogs in small-animal referral hospitals in Japan     

Takaharu Itami  Hiroko Aida  Makoto Asakawa  Yoko Fujii  Tomoya Iizuka  Ayako Imai  Toshie Iseri  Tomohito Ishizuka  Kei Kakishima  Masatoshi Kamata  Takako Miyabe-Nishiwaki  Shotaro Nagahama  Kiyokazu Naganobu  Ryohei Nishimura  Shozo Okano  Tadashi Sano  Kazuto Yamashita  Yoshiki Yamaya  Masashi Yanagawa 《Veterinary anaesthesia and analgesia》2017,44(3):461-472

Objective

To explore the major risk factors linking preoperative characteristics and anaesthesia-related death in dogs in referral hospitals in Japan.

Study design

Observational cohort study.

Animals

From April 1, 2010 to March 31, 2011, 4323 dogs anaesthetized in 18 referral hospitals in Japan.

Methods

Questionnaire forms were collated anonymously. Death occurring within 48 hours after extubation was considered as an anaesthesia-related death. Patient outcome (alive or dead) was set as the outcome variable. Preoperative general physical characteristics, complete blood cell counts, serum biochemical examinations and intraoperative complications were set as explanatory variables. The risk factors for anaesthesia-related death were evaluated using chi-square test or Fisher's exact test, followed by multivariable logistic regression analysis of the data. Significance was set at p < 0.05.

Results

Thirteen dogs that died from surgical error or euthanasia were excluded from statistical analysis. The total mortality rate in this study was 0.65% [28/4310 dogs; 95% confidence interval (CI), 0.41–0.89]. Furthermore, 75% (95% CI, 55.1–89.3) of anaesthesia-related deaths occurred in dogs with pre-existing diseases. Most of the deaths occurred postoperatively (23/28; 82.1%; 95% CI, 63.1–93.9). Preoperative serum glucose concentration <77 mg dL^–1 (6/46; 13.0%; 95% CI, 4.9–26.3), disturbance of consciousness (6/50; 12.0%; 95% CI, 4.5–24.3), white cell count >15,200 μL^–1 (16/499; 3.4%; 95% CI, 1.9–5.5) and American Society of Anesthesiologists grade III–V (19/1092; 1.7%; 95% CI, 1.1–2.7) were identified as risk factors for anaesthesia-related death. Intraoperative hypoxaemia (8/34; 23.5%; 95% CI, 10.7–41.2) and tachycardia (4/148; 2.7%; 95% CI, 0.7–6.8) were also risk factors for anaesthesia-related death.

Conclusions and clinical relevance

The results revealed that certain preoperative characteristics were associated with increased odds of anaesthesia-related death, specifically low serum glucose concentration and disturbances of consciousness. Greater attention to correcting preanaesthetic patient abnormalities may reduce the risk of anaesthesia-related death.  相似文献   

Self-assembled hexa-peri-hexabenzocoronene graphitic nanotube     

Hill JP  Jin W  Kosaka A  Fukushima T  Ichihara H  Shimomura T  Ito K  Hashizume T  Ishii N  Aida T 《Science (New York, N.Y.)》2004,304(5676):1481-1483

An amphiphilic hexa-peri-hexabenzocoronene self-assembles to form a pi-electronic, discrete nanotubular object. The object is characterized by an aspect ratio greater than 1000 and has a uniform, 14-nanometer-wide, open-ended hollow space, which is an order of magnitude larger than those of carbon nanotubes. The wall is 3 nanometers thick and consists of helical arrays of the pi-stacked graphene molecule, whose exterior and interior surfaces are covered by hydrophilic triethylene glycol chains. The graphitic nanotube is redox active, and a single piece of the nanotube across 180-nanometer-gap electrodes shows, upon oxidation, an electrical resistance of 2.5 megohms at 285 kelvin [corrected]. This family of molecularly engineered graphite with a one-dimensional tubular shape and a chemically accessible surface constitutes an important step toward molecular electronics.  相似文献   

Influence of quinidine and flecainide on autonomic nervous activity in thoroughbred horses     

Ohmura H  Hiraga A  Aida H  Kuwahara M  Tsubone H 《The Veterinary record》2003,152(4):114-116

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In situ hybridization for the demonstration of bovine leukemia virus transcripts in lymphosarcoma cells using biotinylated probes.     

K Okada  Y Hosokawa  Y Aida  K Ohshima 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1991,38(9):707-713

Bovine leukemia virus (BLV) RNA was demonstrated in peripheral blood lymphocytes treated with concanavalin A or phytohemagglutinin from a BLV-infected ox, and in lymphosarcoma cells cultured without mitogen from an enzootic bovine leukosis cow by in situ hybridization using biotinylated DNA probes.  相似文献   

Isolation of Nonpathogenic Mutants of Fusarium oxysporum f. sp. melonis for Biological Control of Fusarium Wilt in Cucurbits     

Freeman S  Zveibil A  Vintal H  Maymon M 《Phytopathology》2002,92(2):164-168

ABSTRACT Two nonpathogenic mutant strains 4/4 and 15/15 of Fusarium oxysporum f. sp. melonis (race 1,2) were isolated by a continuous dipinoculation technique following UV mutagenesis of the virulent wild-type isolate FOM1.2. No disease symptoms or detrimental effects were observed following inoculation of muskmelon seedlings by strain 4/4. In contrast, strain 15/15 caused mortality of susceptible cultivars although to a lesser extent than the wild-type isolate. Strain 4/4 colonized a variety of muskmelon and watermelon cultivars. In muskmelon cv. Ein Dor, seedlings were dipped in a conidial suspension of strain 4/4 and planted in medium amended with the mutant to achieve 100% colonization of roots and between 30 to 70% of the lower stem tissues 7 days after planting. Similar percent colonization of watermelon seedlings by strain 4/4 was recorded. In cross-protection experiments with muskmelon cultivars, significant reduction in seedling mortality was observed between 4/4-colonized FOM1.2. challenged plants compared with that of wild-type challenged plants alone. Similarly, strain 4/4 was able to significantly reduce mortality of watermelon seedlings caused by F. oxysporum f. sp. niveum race 2. This novel approach of generating nonpathogenic mutants for biological control in Fusarium spp. and other fungal pathogens from virulent wild-type isolates may be beneficial for control, because the mutant strains, lacking only in pathogenicity, may compete more efficiently than other biocontrol organisms against the pathogen of origin.  相似文献