Antigen-specific histamine release in dogs with food hypersensitivity     

Ishida R  Masuda K  Sakaguchi M  Kurata K  Ohno K  Tsujimoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(3):435-438

An in vitro evidence of IgE-mediated hypersensitivity to food allergens was detected by positive results of antigen-specific histamine release in dogs with food hypersensitivity. Eight dogs were diagnosed to have food hypersensitivity based on identification of offending food allergens with food elimination followed by oral food provocation. The percentages of histamine release against the stimulation of offending food allergens in the cases ranged from 2.1% to 70.9%. Six of the 8 cases showed histamine release higher than those of healthy control dogs. Four dogs showed relatively high histamine release at the percentage beyond 10% that was compatible with a positive value of histamine release in humans with food hypersensitivity. These findings would suggest that IgE-mediated hypersensitivity against food allergens could be involved in canine food hypersensitivity.  相似文献   

Association between changes in plasma calcium concentration and plasma tartrate-resistant acid phosphatase activity in periparturient cows     

Sato J  Sato R  Takagi A  Goto T  Okada K  Yasuda J  Naito Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(2):291-293

Eight periparturient Holstein Friesian cows were examined for plasma tartrate-resistant acid phosphatase (TRAP) activity to assess the degree of bone metabolic activity and to evaluate the association between the change in calcium (Ca) concentration and bone metabolism during the periparturient period. Milk fever occurred in 1 of 8 cows just after parturition. Plasma TRAP activities did not markedly change in 5 of 8 cows during the experimental period. The changing rate of Ca between preparturition and just after parturition was under -20% in 3 of 8 cows, and low TRAP activities were observed in 2 of these 3 cows. This study suggests that cows with a low TRAP activity are at risk of developing milk fever in comparison to cows with high TRAP activity. Temporary increases of parathyroid hormone were observed in 7 cows, but not in the cow with milk fever.  相似文献   

Increase in apoptotic polymorphonuclear neutrophils in peripheral blood after intramammary infusion of Escherichia coli lipopolysaccharide     

Yagi Y  Shiono H  Shibahara T  Chikayama Y  Nakamura I  Ohnuma A 《Veterinary immunology and immunopathology》2002,89(3-4):115-125

A transient increase in apoptotic polymorphonuclear neutrophils (PMNs) as revealed by the terminal deoxynucleotidyl, transferase-mediated dUTP nick end labeling (TUNEL) technique in bovine jugular and milk vein blood was observed 4 h after intramammary infusion of Escherichia coli lipopolysaccharide (LPS) (jugular vein; before infusion 10.1%, 4h 58.3%: milk vein; before infusion 13.2%, 4 h 76.6%) decrease in PMA-induced oxidative bursts of PMNs was also observed during the same period and continued until 8 h after the infusion. TUNEL-positive cells showed an intention of a Comet tail as detected by a single-cell gel electrophoresis assay (Comet assay) and the morphological apoptotic future, though DNA fragmentation was not clearly detected. A definite decrease in peripheral PMNs and a marked increase in PMNs in the LPS-infused teat cistern were observed during the same period. The migration of milk vein blood-derived PMN and the expression of adhesion receptors (L-selectin and CD18) on PMN were suppressed, accompanied by an increase in apoptotic cells. TUNEL-positive PMN observed in normal animals showed a reduced migration capacity. The increase in apoptotic PMNs observed in the LPS-infused cattle was thought to be due to the remaining intravenous spontaneous apoptotic cells existing under the normal condition (the aging cell), and this increase appeared to lower the expression of adhesion receptors and the migration capacity. Decreased PMA-induced oxidative burst activity in PMN was thought to be derived from these aging cells and immature band cells appearing in the circulation as a subsequent event of leukopenia and/or severe stress associated with mastitis. The results from the present study indicate the possibility that the function of PMN in the circulation at early stages of bovine mastitis is regulated by the kinetics of PMN aging.  相似文献   

Development of novel clubroot resistant rapeseed lines (Brassica napus L.) effective against Japanese field isolates by marker assisted selection     

Mitsuyo Kawasaki  Takayoshi Ohara  Masahiko Ishida  Yoshihito Takahata  Katsunori Hatakeyama 《Breeding Science》2021,71(5):528

Clubroot is an important disease infectible to cruciferous plants and a major threat to rapeseed production in Japan. However, no clubroot resistant rapeseed cultivars have been released. We surveyed pathotype variation of six isolates collected from rapeseed fields and found they were classified as pathotype groups 2 and 4 using Japanese F₁ Chinese cabbage cultivars. We produced the resynthesized clubroot resistant Brassica napus harboring two resistant loci, Crr1 and Crr2, by interspecific crossing and developed resistant rapeseed lines for southern and northern regions by marker-assisted selection and backcrossing. We improved the DNA marker for erucic acid content to remove linkage drag between Crr1 and high erucic acid content and successfully selected lines with clubroot resistance and zero erucic acid for northern regions. A novel line, ‘Tohoku No. 106’, suitable for southern regions showed stable resistance against all six isolates and high performance in infested fields. We conclude that Crr1 and Crr2 are important genes for CR rapeseed breeding and marker-assisted selection is effective in improving clubroot resistance.  相似文献   

Identification of enzyme genes in the liver of the Bleeker’s squid Loligo bleekeri by expressed sequence tag analysis     

Hidehiro Kondo  Takami Morita  Maki Ikeda  Chihiro Kurosaka  Aiko Shitara  Yuka Honda  Reiko Nozaki  Takashi Aoki  Ikuo Hirono 《Fisheries Science》2010,76(1):161-165

Expressed sequence tag (EST) analyses were performed with the aim of identifying enzyme genes in the liver of the Bleeker’s squid Loligo bleekeri. Of the 768 ESTs identified and sequenced, 669 were grouped into 324 clusters. Of these clusters, 123 comprising 245 ESTs were found to be homologous to genes reported to date. Among these, 43 clusters were annotated as enzymes according to the Enzyme Commission (EC) numbering system. Two EC groups, oxidoreductases and hydrolases, possessed a large number of ESTs. A cluster homologous to the glutathione peroxidase, an enzyme in the oxidoreductase group, contained 16 ESTs, which accounted for 2.4% of the total ESTs sequenced. There are three serine proteases, three cathepsins, two triacylgricerol lipases, and two chitinases among the clusters homologous to the enzymes in the hydrolase group. Since the squid liver functions in the digestive process, these enzymes would be involved in food digestion. Our data provide information on the various types of enzymes expressed in the squid liver and may provide a useful basis for further characterization of these enzymes.  相似文献   

Transverse shape analysis of xylem ground tissues by Fourier transform image analysis I: trial for statistical expression of cell arrangements with fluctuation     

Yoko Midorikawa  Yoji Ishida  Minoru Fujita 《Journal of Wood Science》2005,51(3):201-208

Periodic cell arrangements of tracheids in Cryptomeria japonica and Agathis sp., and that of wood fibers in Magnolia obovata were examined by Fourier transform image analysis (FTIA). The angular distribution functions on power spectral patterns (PSPs) transformed from the dot maps were traced. As well as this conventional method, we developed the line convolution method and domain transformation for more correct analysis of radial and tangential arrangements. Cell arrangements became clear in Cryptomeria and Agathis. Fluctuations were expressed by the standard deviation (σ) or the relative standard deviation (σ_r) so that we could compare the characteristics of both woods. In Cryptomeria, the most provable distance between tracheids along radial files was 36.7 µm with 17% fluctuation, whereas the tangential interval was 28.3 µm with σ_r of 24%. In Agathis, the radial diameter was 35.1 µm with σ_r of 23% and the tangential interval was 41.5 µm with σ_r of 23%. In Magnolia, the maximal periodicity of wood fibers was 15.8 µm and 20.5 µm along and between radial files, respectively, although the fluctuations could not be estimated because of the remarkable interruptions by numerous vessels.  相似文献   

Vertical movement of a chum salmon Oncorhynchus keta in the western North Pacific Ocean as determined by a depth-recording archival tag     

Yukimasa Ishida  Akira Yano  Masatoshi Ban  Miki Ogura 《Fisheries Science》2001,67(6):1030-1035

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A comparison of the concentrations of C-reactive protein and alpha1-acid glycoprotein in the serum of young and adult dogs with acute inflammation     

Hayashi S  Jinbo T  Iguchi K  Shimizu M  Shimada T  Nomura M  Ishida Y  Yamamoto S 《Veterinary research communications》2001,25(2):117-126

The concentrations of C-reactive protein (CRP) and alpha1-acid glycoprotein (AAG) were evaluated in 1-, 3- and 18-month-old dogs (four of each age) that had been inoculated with turpentine oil. The CRP and AAG in 3-month-old and younger dogs subjected to surgery or inoculated with either Staphylococcus aureus or a viral vaccine were also evaluated. The average CRP concentration in the sera peaked 2 days after inoculation of turpentine oil. The peak CRP concentrations in 3- and 18-month-old dogs were significantly (p < 0.05) greater than those in 1-month-old dogs. The average AAG concentration in the sera peaked 4 days after inoculation of turpentine oil. No significant difference was found in AAG concentrations between any of the age groups. When experimentally inoculated with S. aureus or subjected to oophorohysterectomy, the CRP and AAG concentrations increased in 3-month-old dogs, but they increased little in 1-month-old dogs. The CRP and AAG in dogs inoculated with the viral vaccine did not increase. In dogs with fractures or subjected to percutaneous gastrostomy, the CRP and AAG concentrations correlated with the condition of dogs.  相似文献   

Efficacy of Malarone? in Dogs Naturally Infected with Babesia gibsoni     

Aiko IGUCHI  Nobuyuki SHIRANAGA  Aya MATSUU  Yoshiaki HIKASA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(9):1291-1295

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Itokawa dust particles: a direct link between S-type asteroids and ordinary chondrites     

Nakamura T  Noguchi T  Tanaka M  Zolensky ME  Kimura M  Tsuchiyama A  Nakato A  Ogami T  Ishida H  Uesugi M  Yada T  Shirai K  Fujimura A  Okazaki R  Sandford SA  Ishibashi Y  Abe M  Okada T  Ueno M  Mukai T  Yoshikawa M  Kawaguchi J 《Science (New York, N.Y.)》2011,333(6046):1113-1116

The Hayabusa spacecraft successfully recovered dust particles from the surface of near-Earth asteroid 25143 Itokawa. Synchrotron-radiation x-ray diffraction and transmission and scanning electron microscope analyses indicate that the mineralogy and mineral chemistry of the Itokawa dust particles are identical to those of thermally metamorphosed LL chondrites, consistent with spectroscopic observations made from Earth and by the Hayabusa spacecraft. Our results directly demonstrate that ordinary chondrites, the most abundant meteorites found on Earth, come from S-type asteroids. Mineral chemistry indicates that the majority of regolith surface particles suffered long-term thermal annealing and subsequent impact shock, suggesting that Itokawa is an asteroid made of reassembled pieces of the interior portions of a once larger asteroid.  相似文献