Managing lateral infiltration on wide beds in clay and sandy clay loam using Hydrus 2D     

Ghani Akbar  Steven Raine  Allen David McHugh  Greg Hamilton 《Irrigation Science》2015,33(3):177-190

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Inhibition of P-glycoprotein ATPase and its transport function of Helicoverpa armigera by morin, quercetin and phloroglucinol     

Ravindra M. Aurade  S.MD. Akbar  Hanumanth GoudSenigala K. Jayalakshmi  Kuruba Sreeramulu 《Pesticide biochemistry and physiology》2011,101(3):212-219

Flavonoids (morin, quercetin and phloroglucinol) were tested for their ability to modulate the function of P-glycoprotein ATPase of the insecticide resistant pest Helicoverpa armigera (Ha-Pgp). Flavonoids in the presence of ethylparaoxon or cypermethrin significantly reduced both larval weight as well as survival rate 40-50%. Morin and quercetin inhibited the activity of Ha-Pgp ATPase by 80-90%, whereas phloroglucinol inhibited ATPase activity by 40% at 100 μM concentration. These flavonoids inhibited the verapamil, ethylparaoxon and cypermethrin-stimulated Ha-Pgp ATPase activity. Morin, quercetin and phloroglucinol binding were quantitated by quenching of the intrinsic Trp fluorescence of purified Ha-Pgp ATPase. Drug transport was monitored in proteoliposomes containing Ha-Pgp ATPase using the high affinity fluorescent substrate tetramethylrosamine (TMR) in real time. Addition of the morin and quercetin mediated the collapse of the TMR concentration gradient generated by Ha-Pgp ATPase. The inhibition studies on Ha-Pgp ATPase activity may contribute towards understanding new strategies of the pest to overcome insecticide resistance.  相似文献   

Characterization of <Emphasis Type="Italic">Pectobacterium carotovorum</Emphasis> causing a new soft rot disease on okra in Malaysia     

Eisa Nazerian  Kamaruzaman Sijam  Zainal Abidin Meor Ahmad  Kavous Keshavarz 《Journal of General Plant Pathology》2011,77(5):292-294

Dark brown, necrotic pods with extensive water-soaked lesions caused by plant pathogenic bacteria were found on okra plants in different fields in Malaysia in 2010. PCR amplification of the pectate lyase (pel) gene and amplification of the 16S–23S rRNA (ITS) with G₁ and L₁ primers produced 434-, 535- and 570-bp fragments, respectively. From the similarity between the results of biochemical tests and their equivalency with standard bacteriological sources, PCR-based pel gene, and RFLP analysis of the ITS-PCR products, all isolates were identified as Pectobacterium carotovorum. This is the first report of P. carotovorum in okra from Malaysia.  相似文献   

Subtype analysis of Cryptosporidium parvum and Cryptosporidium hominis isolates from humans and cattle in Iran     

Nazemalhosseini-Mojarad E  Haghighi A  Taghipour N  Keshavarz A  Mohebi SR  Zali MR  Xiao L 《Veterinary parasitology》2011,179(1-3):250-252

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白斑狗鱼StAR基因克隆及其表达分析          下载免费PDF全文

张俊杰  李菁  赵瑞阳  古丽帕日·艾克拜 《南方农业学报》2021,52(11):3093-3101

【目的】克隆白斑狗鱼（Esox lucius）类固醇激素合成急性调节蛋白（StAR）基因（ElStAR）并分析其组织表达差异,为开展StAR基因生物学功能研究及揭示白斑狗鱼性腺发育机制提供基础资料。【方法】根据GenBank已公布的白斑狗鱼基因组测序结果（NC_047581.1）设计特异性引物,采用RT-PCR克隆ElStAR基因cDNA序列,通过ClustalX、ExPASy、TargetP 1.1、TMHMM 2.0和SignalP 5.0等在线软件进行生物信息学分析,并采用实时荧光定量PCR进行ElStAR基因组织表达定量分析。【结果】ElStAR基因cDNA序列长度1485 bp,其开放阅读框（ORF）为864 bp,共编码287个氨基酸残基;ElStAR氨基酸序列与北极红点鲑StAR氨基酸序列的相似性最高（93.33%）,与海鳟、条纹鲈鱼、金头鲷、大菱鲆、斑马鱼、半滑舌鳎的相似性均高于75.00%,而与小家鼠的相似性最低（59.15%）;基于StAR氨基酸序列相似性构建的系统发育进化树也显示白斑狗鱼与北极红点鲑和海鳟等鲑科鱼类处于同一分支。ElStAR蛋白相对分子量为32.23 kD,理论等电点（pI）为8.98,为不稳定的亲水性蛋白;具有START结构域,无信号肽及跨膜结构,符合线粒体靶向肽的基本特征。ElStAR蛋白二级结构由α-螺旋（占40.77%）、无规则卷曲（占35.89%）、延伸链（占18.12%）和β-折叠（占5.23%）组成,其三级结构是由α-螺旋配合多个β-折叠卷曲盘旋而成。ElStAR基因在白斑狗鱼精巢、卵巢、头肾、肝脏、肌肉及脑组织中均有不同程度的表达,且在精巢中的相对表达量极显著高于在卵巢中的相对表达量（P<0.01）,呈明显的性别二态性表达模式。【结论】ElStAR基因编码蛋白结构和功能十分保守,具有典型的START结构域,对胆固醇的运输和调节起重要作用。ElStAR基因在白斑狗鱼精巢及卵巢中的表达存在极显著差异,可能在维持白斑狗鱼精巢的发育形成中发挥重要作用。  相似文献   

A novel eco-friendly colorant and dyeing method for poly(ethylene terephthalate) substrate     

Gorkem Gedik  Ozan Avinc  Arzu Yavas  Akbar Khoddami 《Fibers and Polymers》2014,15(2):261-272

Although madder (Rubia tinctorum) has been used as a well-known natural textile dye source for dyeing of natural fibers such as wool, silk and cotton, 100 % polyester dyeing with madder is not common. In this study, polyester samples were dyed with madder at different dyeing temperatures, from 60 °C to 130 °C, in company with 7 chemical and 5 natural mordants. Color properties and rub, light and wash fastness performances were investigated. Different shades of orange, brown, pink and reddish green colors were obtained. Dyeing at 130 °C exhibited the highest color yield, the highest chroma and the lowest lightness values. Overall, chemical mordants exhibited higher color yields than natural mordants. High wash fatness, moderate light and rub fastness levels were observed. The potassium bitartrate and gallnut, a natural mordant, exhibited the best results. The madder dyeing of polyester should be carried out at hot temperature conditions, 130 °C, in order to obtain the highest achievable color yield and chroma with the highest light and wash fastness properties.  相似文献   

Optimal isotherm model and explanatory characteristics associated with metal ion adsorption on humic acids isolated from forest soils     

Fazeli Sangani  Mahmood  Forghani  Akbar  Boguta  Patrycja  Anoosha  Marjan  Owens  Gary 《Journal of Soils and Sediments》2022,22(9):2392-2405

Journal of Soils and Sediments - Understanding the mechanisms of interaction between humic acids (HAs) and metal ions in soil media is essential for integrated environmental and agricultural...  相似文献   

Shift work as an oxidative stressor     

Akbar?SharifianEmail author  Saeed?Farahani  Parvin?Pasalar  Marjan?Gharavi  Omid?Aminian 《Journal of circadian rhythms》2005,3(1):15

Background  

Some medical disorders have higher prevalence in shift workers than others. This study was designed to evaluate the effect of night-shift-working on total plasma antioxidant capacity, with respect to the causative role of oxidative stress in induction of some of these disorders.  相似文献   

Long-term land-use change effects on phosphorus fractionation in Zrêbar Lake margin soils     

Mohsen Sheklabadi  Hamid Mahmoudzadeh  Ali Akbar Mahboubi  Bahram Gharabaghi  Beau Ahrens 《Archives of Agronomy and Soil Science》2013,59(6):737-749

The aim of this study was to survey long-term changes in phosphorus (P) fractions in soils in different land uses. Sequential extraction had been used to determine soil P fractionation on the basis of land-use change, i.e. native forest to vineyard and wetland to both alfalfa and wheat at the end of 30 and 20 years, respectively. The highest values of labile-P (L-P) fractions in surface soil layers were observed in the cultivated land. The calcium-bound P was the most affected fraction by land-use change with the lowest amount in the vineyard and the highest amount in the alfalfa land. Conversion of forest to vineyards causes P in the soils to be more vulnerable to transmission; thus, the least amount of total P (T-P) in vineyard may be attributed to the removal of sediment and sediment bond P from runoff in response to land-use changes. Average L-P in alfalfa land was two times more than that in vineyard. Results showed that 44.5% of T-P in native land was stored in surface layer; besides, the portion of the arable surface horizon in T-P sequestration was 33%.  相似文献   

A model to predict the dry matter and yield of rapeseed under salinity and deficit irrigation     

Ali Shabani  Ali Reza Sepaskhah  Ali Akbar Kamgar-Haghighi 《Archives of Agronomy and Soil Science》2013,59(4):525-542

In this investigation, a model was developed to predict dry matter, seed yield and other crop parameters of rapeseed under deficit irrigation and salinity by using soil water and salt budget and other simple plant physiological relationships. Two-year experimental data were used. In calibration and validation of the presented model, results indicated that the model was able to estimate evapotranspiration, soil water content, leaf area index, evaporation, crop transpiration, dry matter and seed yield of rapeseed properly. The advantage of this model is its simplicity and easy calibration in other areas and climate conditions and it can be used to estimate yield and other crop parameters with common measurable data in the field. Prediction of crop yield by this model can be used for better management of agronomic systems to reduce administrative costs and in different environmental conditions. Finally, under scarce data, arid and semi-arid environments, this model is proposed to be used by irrigation managers and agricultural advisors.  相似文献