Preferential gene transcription of T helper 2 cytokines in peripheral CCR4+CD4+ lymphocytes in dogs     

Aki Iio  Tsutomu Motohashi  Takahiro Kunisada  Yuma Yasuhira  Hiroaki Kamishina  Sadatoshi Maeda 《Veterinary dermatology》2014,25(3):199-e50

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Inhibition of p16 tumor suppressor gene expression via promoter hypermethylation in canine lymphoid tumor cells     

Aki Fujiwara-Igarashi  Yuko Goto-KoshinoHiroyuki Mochizuki  Masahiko SatoYasuhito Fujino  Koichi OhnoHajime Tsujimoto 《Research in veterinary science》2014

To investigate the epigenetic regulation of the p16 gene in canine lymphoid tumor cells, its methylation status was examined in four canine lymphoid tumor cell lines. In three canine lymphoid tumor cell lines (CLBL-1, GL-1, and UL-1) with low-level p16 mRNA expression, 20 CpG sites in the promoter region of p16 gene were consistently methylated although all of the CpG sites were not methylated in another cell line (CL-1) and normal lymph node cells. The expression level of p16 mRNA in these three cell lines was restored after cultivation in the presence of a methylation inhibitor, 5-Aza-2′-deoxycitidine, indicating inactivation of p16 gene via hypermethylation. This study revealed the inactivation of p16 gene through hypermethylation of its CpG island in a fraction of canine lymphoid tumor cells.  相似文献   

Total plant sterols,steryl ferulates and steryl glycosides in milling fractions of wheat and rye     

Laura Nyström  Aki PaasonenAnna-Maija Lampi  Vieno Piironen 《Journal of Cereal Science》2007

The total plant sterol, steryl ferulate and steryl glycoside contents in wheat and rye milling fractions show that there are significant differences in both sterol content and composition between various milling fractions collected from a commercial mill. Total sterols were analysed by gas chromatography after acid and alkaline hydrolyses. The steryl conjugates were first extracted with acetone, fractionated on solid phase extraction (SPE) cartridges and then analysed individually (steryl glycosides by gas chromatography and steryl ferulates by reverse phase-high performance liquid chromatography with UV detection. Differences in sterol contents of the wheat samples were greater than in the rye samples. The highest total sterol content was found in wheat germ, but surprisingly high sterol contents, that were comparable to bran, were found in some flour fractions. Contents of steryl ferulates were high in the bran fractions contributing up to 17% of total sterols. The variation in the content of steryl glycosides in the samples was lower and contributed less than 10% of total sterols. These results show that much of the bioactive components may be lost when certain flour fractions produced in common flour milling procedures are discarded. However, some of these fractions with significantly high sterol contents could possibly be introduced into milling products used in breadmaking and the food industry without greatly compromising consumer acceptability.  相似文献