Deformed liver with prominent proliferation of bile ducts in a pig     

Sugiyama A  Ozaki K  Narama I 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(5):655-658

A deformed liver characterized by remarkable ductular proliferation was encountered in a 6-month-old pig and examined histopathologically. The most conspicuous histopathologic change was a mild to severe ductular proliferation in the interlobular areas without any degenerative changes of cholangiolar epithelial cells or hepatocytes. Fibrotic changes and reconstruction of the lobule were not found. Morphological evidence of intrahepatic and extrahepatic cholestasis was lacking. Other characteristics were deformity with displacement of the gall bladder, irregular shape and size of lobules, and structural abnormality of large-sized vessels. The severe ductular proliferation was considered to be due to structural malformations of the excretion channel of bile.  相似文献   

Histone H4 gene expression and morphological changes on shoot apices of strawberry (Fragaria × ananassa Duch.) during floral induction     

Takeshi Kurokura  Yukio Inaba  Nobuo Sugiyama 《Scientia Horticulturae》2006

To investigate flower induction in June-bearing strawberry plants, morphological changes in shoot apices and Histone H4 expression in the central zone during flower initiation were observed. Strawberry plants were placed under flower inducible, short-day conditions (23 °C/17 °C, 10 h day length) for differing number of days (8, 16, 20, 24 or 32 days) and then these plants were transferred to non-inducible, long-day conditions (25 °C/20 °C, 14 h day length). The shoot apices of plants placed under short-day conditions for 8 days were flat, similar to shoot apices of plants in the vegetative phase of development, and Histone H4 was not expressed in the central zone during the experimental period. On the other hand, the shoot apices of plants placed under short-day conditions for 16 days remained flat, similar to shoot apices of plants placed under short-day conditions for 8 days, but Histone H4 was expressed in the central zone at the end of the short-day treatment. Morphological changes in the shoot apices of these plants were observed 8 days after the change in day-length. These plants developed differentiated flower organs after they were grown for another 30 days under long-day conditions. These results indicate that changes in the expression pattern of the Histone H4 gene occur before morphological changes during flower induction and that the expression of the gene in the central zone can be used as one of the indicators of the flowering process in strawberries.  相似文献   

A Point Mutation in the Two-Component Histidine Kinase BcOS-1 Gene Confers Dicarboximide Resistance in Field Isolates of Botrytis cinerea     

Oshima M  Fujimura M  Banno S  Hashimoto C  Motoyama T  Ichiishi A  Yamaguchi I 《Phytopathology》2002,92(1):75-80

ABSTRACT Partial DNA fragments of Botrytis cinerea field isolates encoding the putative osmosensor histidine kinase gene (BcOS1) were cloned by polymerase chain reaction amplification and the predicted amino acid sequences were compared between dicarboximide-sensitive and resistant field isolates. The predicted BcOS1p is highly homologous to osmosensor histidine kinase OS1p from Neurospora crassa including the N-terminal six tandem repeats of approximately 90 amino acids. Four dicarboximide-resistant isolates of B. cinerea (Bc-19, Bc-45, Bc-682, and Bc-RKR) contained a single base pair mutation in their BcOS1 gene that resulted in an amino acid substitution in the predicted protein. In these resistant isolates, codon 86 of the second repeat, which encodes an isoleucine residue in sensitive strains, was converted to a codon for serine. The mutation of Botrytis field resistant isolates was located on the second unit of tandem amino acid repeats of BcOS1p, whereas the point mutations of the fifth repeat of OS1p confer resistance to both dicarboximides and phenylpyrroles and also osmotic sensitivity in Neurospora crassa. These results suggest that an amino acid substitution within the second repeat of BcOS1p is responsible for phenotypes of field resistant isolates (resistant to dicarboximides but sensitive to phenylpyrroles, and normal osmotic sensitivity) in B. cinerea.  相似文献   

Primary malignant peripheral nerve sheath tumor with eosinophilic cytoplasmic globules arising from the greater omentum in a dog     

Sugiyama A  Morita T  Shimada A  Tsuka T  Okamoto Y  Takeuchi T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(7):739-742

A 10-year-old Golden Retriever dog had a solitary tumor mass arising from the greater omentum. Histologically, the tumor showed varying cellularity and patterns of cellular arrangement. In dense cellular areas, spindle-shaped cells were arranged in interlacing bundles. The sparse cellular area was characterized by loosely arranged fusiform cells. The neoplastic cells frequently contained PAS-positive eosinophilic globules in the cytoplasm, and mitotic figures were frequently observed. The tumor cells were positive to vimentin, S-100 protein, glial fibrillary acidic protein, myelin basic protein, neuron-specific enolase and myoglobin. The present tumor was diagnosed as a malignant peripheral nerve sheath tumor (MPNST) with eosinophilic cytoplasmic globules arising from the greater omentum. To our knowledge, this may be the first case of primary omental MPNST in domestic animals.  相似文献   

Trans-endothelial and trans-epithelial transfer of lactoferrin into the brain through BBB and BCSFB in adult rats     

Kamemori N  Takeuchi T  Sugiyama A  Miyabayashi M  Kitagawa H  Shimizu H  Ando K  Harada E 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(3):313-315

The transportation of intravenously administered bovine lactoferrin (bLF) into the cerebrospinal fluid (CSF) was immunohistochemically investigated in adult rats. Administered bLF was detected in the vesicular membranes of endothelial cells in cerebral blood vessels 10 min after the infusion. Numerous immunoreactive small vesicles were also detected at the ependymal cells in the choroid plexus. Moreover, the bLF concentration in the CSF was significantly increased at 1-2 hr after the intravenous infusion of bLF (10 or 30 mg/kg). These findings clearly demonstrate that LF is possibly transported into the brain matter even in adult animals.  相似文献   

Review of population dynamics and management of small pelagic fishes around the Japanese Archipelago     

Yatsu  Akihiko 《Fisheries Science》2019,85(4):611-639

Fisheries Science - Population dynamics of small pelagic fishes (SPF) and their management in Japan were reviewed for Japanese sardine Sardinops melanostictus, Japanese anchovy Engraulis japonicus,...  相似文献   

Monthly changes in the serum levels of two choriogenins and vitellogenin in male masu salmon <Emphasis Type="Italic">Oncorhynchus masou</Emphasis>     

Toshiaki Fujita  Haruhisa Fukada  Munetaka Shimizu  Naoshi Hiramatsu  Akihiko Hara 《Fisheries Science》2008,74(5):1198-1200

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Changes in the population of lymphocytes expressing CD4 and CD8 during the process of atresia of white follicles in hens     

Yukinori YOSHIMURA  Akihiko KITAMURA 《Animal Science Journal》2002,73(6):457-463

The aim of this study was to determine whether the population of lymphocytes expressing CD4 and CD8 molecules changed in the white follicles during atresia in chickens. Frozen sections of healthy, early atretic, advanced atretic and late atretic follicles were immunostained for CD4 and CD8, and the populations of positive cells were analyzed under a light microscope. In the healthy, early atretic and advanced atretic follicles both CD4+ and CD8+ cells were localized in the theca layer, but not in the granulosa layer. However, an influx of CD4+ and CD8+ cells was observed not only in the theca but also in the follicular cavity that was formed by disintegration of the oocyte in late atretic follicles. The frequency of CD4+ T cells in the theca layer did not differ among healthy, early atretic and advanced atretic follicles, but was significantly increased in the late atretic follicles (P < 0.05). The frequency of CD8+ cells showed a pattern of change that resembled that of CD4+ T cells, with a significantly greater population in late atretic follicles than the other follicles (P < 0.05). These results suggest that CD4+ and CD8+ cells are increased in the late atretic follicles, probably to promote the tissue regression.  相似文献   

Experimental study of materials for patch graft on right ventricular outflow tract under extracorporeal circulation in dogs--comparison between Denacol EX-313-treated bovine jugular vein graft and expanded polytetrafluoroethylene (EPTFE) graft     

Matsumoto H  Sugiyama S  Shibazaki A  Tanaka R  Takashima K  Noishiki Y  Yamane Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(9):961-965

A comparison between a bovine jugular vein treated with a hydrophilic polyepoxy compound cross-linker (Denacol), and expanded polytetrafluoroethylene (EPTFE), an artificial material, as a patch graft for the reconstruction of the right ventricular outflow tract under extracorporeal circulation in dogs, as if they had pulmonic stenosis, was made. Hemodynamic and histological examinations were conducted two weeks after the transplantation. Hemodynamic problems were not observed in either the Denacol or EPTFE groups. Macroscopically, organization of new tissue on the graft surface was more marked in the EPTFE group than in the Denacol group, and newly-formed tissue was seen surrounding the border of the graft and burying it in the EPTFE group. In the Denacol group, microscopic findings revealed the presence of inflammatory cells and fibroblasts, and an invasion of the graft by collagen fibers and elastic fibers. In the EPTFE group, there was minimal cellular infiltration of the graft and a thick layer consisting of collagen fibers and fibroblasts was observed around the graft. These results indicated that two weeks after transplantation the graft was better assimilated and organized with blood vasculature in the patch graft in the Denacol group than in the EPTFE group.  相似文献   

Attachment and infection to MA104 cells of avian rotaviruses require the presence of sialic acid on the cell surface     

Sugiyama M  Goto K  Uemukai H  Mori Y  Ito N  Minamoto N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(4):461-463

To determine the characters of receptors on target cells for avian rotaviruses, the receptors on MA104 cells for the pigeon rotavirus PO-13, the turkey rotaviruses Ty-1 and Ty-3, and the chicken rotavirus Ch-1 were analyzed. Pretreatment of MA104 cells with neuraminidase greatly reduced the infection by all of the four avian rotavirus strains. Binding of the cell-attachment protein, purified VP8 expressed in bacteria, of strain PO-13 to MA104 cells was also inhibited by pretreatment of cells with neuraminidase. These findings suggest that avian rotaviruses primarily utilize sialic acid-containing molecules as receptors on MA 104 cells.  相似文献