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51.
Doi Y Tamano S Kawabe M Sano M Imai N Nakashima H Furukawa F Hagiwara A Otsuka M Shirai T 《Journal of toxicologic pathology》2011,24(4):207-213
This study was conducted to determine the concordance of results for a pair of structural
isomers, 2-nitropropane (2-NP) and 1-nitropropane (1-NP), using the rat medium-term liver
carcinogenesis bioassay (Ito test) and previously published long-term carcinogenicity
tests. Male F344 rats were given a single intraperitoneal injection of DEN (200 mg/kg
b.w.) to initiate hepatocarcinogenesis. After 2 weeks, they received per os 0, 0.8, 4 or
20 mg/kg/day of 2-NP or 1-NP six times a week and were subjected to two-thirds partial
hepatectomy at week 3. Non-initiated groups receiving 0 or 20 mg/kg/day were also
included. The animals were sacrificed for quantitative analysis of GST-P-positive foci at
week 8. With the highest dose of 2-NP, significantly increased numbers and areas of
GST-P-positive foci were demonstrated as compared with the respective control but were not
noted with 1-NP. In the non-DEN-initiated groups, many small GST-P-positive foci of less
than 0.2 mm in diameter were also induced in the rats treated with 2-NP at 20 mg/kg/day
but were lacking with 1-NP. These results strongly support that 2-NP is a complete
hepatocarcinogen with a potent initiation activity, whereas 1-NP is not. 相似文献
52.
53.
Akira MATSUDA Ikki MITSUI Yuki SHIMIZU Teppei KANDA Akihiro OHNISHI Masahiro MIYABE Yoshiki ITOH 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2020,82(11):1577
Little is known about the pathological roles of sebaceous glands in canine skin diseases, as most examinations have been conducted with cultured human sebaceous epithelial cell lines. To our knowledge, there is no available canine sebaceous epithelial cell line. The purpose of this study was to establish a canine sebaceous epithelial cell line and characterize it. An eyelid mass in a dog was surgically resected for treatment, and it was histologically diagnosed as sebaceous epithelioma. Collected tissue was conducted for culture, and the growing epithelial-like cells were passaged. The cells showed continuous proliferation for over 6 months. After 40 passages, the cells were named CMG-1. Lipid droplets in the cytoplasm of CMG-1 cells were confirmed by Oil Red O staining. As reported in studies with human sebaceous epithelial cell lines, lipogenesis in CMG-1 cells was promoted by linoleic acid, whereas transforming growth factor-β (TGF-β) suppressed it. Additionally, real-time PCR revealed that the expression levels of chemokines and cytokines, including CC chemokine ligand (CCL)-2, CCL-20, CXCL-10, Tumor necrosis factor-α (TNF-α), Interleukin (IL)-1α, IL-1β, and IL-8, were significantly increased in CMG-1 cells following treatment with lipopolysaccharide. In conclusion, we successfully established a new canine sebaceous epithelial cell line. Our data indicated that lipogenesis and inflammatory responses were quantitatively evaluable in this cell line. CMG-1 cells could be useful for the pathological analysis of sebaceous gland diseases in dogs. 相似文献
54.
Mio KOBAYASHI Toshinori YOSHIDA Risako YAMASHITA Ryo ICHIKAWA Junta NAKAHARA Kazuki NAKAMURA Hiromu OKANO Yasunori TAKAHASHI Nanao ITO Makoto SHIBUTANI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(6):994
We encountered a case of cutaneous squamous cell carcinoma (SCC) in a 17-year-old female koala at a zoo. A fragile, papillary, elevated mass was found on the third digit of the right hind limb. SCC was identified histopathologically: squamous cell-like polygonal tumor cells showed a nest-like growth pattern with epidermal down growth, central keratinization and necrotic foci, and invaded dermal connective tissues. Metastatic lesions were observed in various organs, including the lung and axillary lymph node: in the lung, multiple metastatic foci similar to the primary lesion, and in the axillary lymph node, individual polygonal tumor cells infiltrated the sinusoids. Immunohistochemistry revealed that the tumor cells were positive for proliferating cell nuclear antigen, which exhibited 32–33% of labeling indices in the tumor cells. To our knowledge, this is the first report of a case of SCC in a digit of a koala. 相似文献
55.
Invertase Activities in Sheath-blight Infected Rice Selection Lines and Fungus Culture Filtrates 总被引:2,自引:0,他引:2
Infection of rice plants with Rhizoctonia solani, the sheath blight fungus, at the flowering stage resulted in an increase in acid invertase activity. Activity of the invertase(s)
with optimum pH at 3.5 and 4.5 was higher in the susceptible plants compared to resistant plants. Healthy control plants had
no change in invertase activity. An invertase of fungal origin produced in sheath blight fungus culture filtrates had an optimum
acidic pH of 3.5. The increase in invertase activity observed in the later stages of infection in the susceptible plants compared
to the resistant plants demonstrated a relation to fungal growth. These results suggested that the fungus produced an invertase
for the hydrolysis of sucrose that resulted in alterations of source-sink relationships in the colonized cells. Thus, the
increased invertase activity in the susceptible plants regulated the ratio of hexose to sucrose. This observation is further
supported by the lack of a significant invertase increase in the resistant plants.
Received 14 July 1999/ Accepted in revised form 9 December 1999 相似文献
56.
Qi Li Makoto Osada Masaru Kashihara Ken Hirohashi and Akihiro Kijima 《Fisheries Science》2000,66(4):701-707
57.
Komei HOTTA Takayuki WATANABE Chiho KISHIDA Yukio NAKAMURA Nobuyuki OHKUBO Takahiro MATSUBARA Shinji ADACHI Kohei YAMAUCHI 《Fisheries Science》2003,69(3):555-562
ABSTRACT: Because blood vitellogenin (Vg) has been considered a biomarker for environmental estrogens, the basal levels of Vg and 17β-estradiol (E2 ) were determined in male Japanese whiting reared under natural conditions. Serum levels of Vg and E2 were measured and gonadal development was assessed by gonadosomatic index (GSI) and histological observation in 8–10 male fish at monthly intervals throughout the annual reproductive cycle. Serum E2 was <60 pg/mL throughout the study period. In contrast, serum Vg exhibited seasonal changes: serum levels of Vg gradually increased from April to May (mean 63 ± 13 ng/mL and 124 ± 48 ng/mL in April and May, respectively), and then reached a peak value (mean 352 ± 68 ng/mL) in June. Thereafter, serum Vg gradually decreased, reaching undetectable levels (<50 ng/mL) in October. Serum levels of Vg tended to increase in the male fish in which the GSI was >1%. Histological observation revealed that testes in such male fish were in active spermatogenesis and then all of the testes of male fish in which serum Vg decreased to ND levels were regressed. These results suggest that Vg productive potency (sensitivity to estrogens) may increase in the spermatogenic stage, resulting in production of Vg in response to very low levels of natural or xenobiotic estrogens. 相似文献
58.
59.
Minami Goto Keishi Owaki Akihiro Hirata Tokuma Yanai Hiroki Sakai 《Veterinary and comparative oncology》2019,17(3):285-297
Tumour necrosis factor‐related apoptosis‐inducing ligand (TRAIL) is an apoptosis‐inducing cytokine that shows potential therapeutic value for human neoplasms, and is effective in some canine tumours; however, its potential for killing canine hemangiosarcoma (HSA) cells is unknown. Thus, we evaluated the proapoptotic effect of TRAIL in nine canine HSA cell lines. Cells (JuA1, JuB2, JuB2‐1, JuB4, Re11, Re12, Re21, Ud2 and Ud6) were cultured with three recombinant human TRAILs (rhTRAILs): TRAIL‐TEC derived from Escherichia coli, TRAIL‐TL derived from mammalian cells and isoleucine zipper recombinant human TRAIL (izTRAIL) containing an isoleucine‐zippered structure that facilitates trimerization. TRAIL‐TEC did not decrease the cell viability in any of the cell lines tested, whereas the other two rhTRAILs effectively decreased the viability of all cell lines as assessed by the WST‐1 assay. In canine HSA cells, izTRAIL induced apoptosis more effectively than TRAIL‐TL. In JuB4, Re12, and Ud6 cells, izTRAIL increased the activation of caspase‐3 and caspase‐8 and caused poly (ADP‐ribose) polymerase degradation. Moreover, izTRAIL treatment increased the proportion of Annexin V+/ Propidium iodide (PI)? apoptotic cells and nuclear fragmentation in izTRAIL‐sensitive cells. These results show that rhTRAIL can induce apoptosis in canine HSA cells, but the sensitivity of TRAIL was different depending on the cell lines. Therefore, TRAIL could be an effective therapeutic agent against canine HSA, but the specific mechanism of resistance should be determined to clarify under what conditions this treatment would be most effective. 相似文献
60.
Characterization of myofibrillar adenosine triphosphatase activity and liberation of actin from myofibrils upon heating chicken breast meat 下载免费PDF全文
Masanori Matsuishi Yoshitaka Eda Emi Saito Shohei Yamamoto Kenji Kanamori Yuto Goto Yutaro Kobayashi Akihiro Okitani 《Animal Science Journal》2018,89(3):597-605
Denaturation of actin and myosin in myofibrils induced by heating at 50°C was investigated to reveal the mechanism of irreversible liberation of actin from myofibrils on heating at lower temperatures than conventional cooking. Denaturation of these proteins was determined by Mg2+‐ATPase (adenosine triphosphatase) and Ca2+‐ATPase activities. When minced meat was heated for 20 min, actin was liberated accompanying denaturation of 80% of actin and 50% of myosin. Heating of the myofibrillar fraction (MFF) isolated from meat homogenate induced much slower denaturation of actin than myosin. When MFF was heated with sarcoplasmic fractions, denaturation of actin was facilitated, suggesting that sarcoplasmic fractions contain factors to facilitate actin denaturation. Inosine‐5′‐monophosphate, a component of sarcoplasmic fractions, was shown to have no effect on actin and myosin denaturation. These results suggest that heating meat at 50°C dissociates binding (‘Bond A’) between actin and myosin participating in ATPase activities, resulting in denaturation of both proteins under influence of sarcoplasmic components. Although denaturation of actin and myosin disrupted Bond A, actin was not liberated simultaneously, suggesting the presence of another bond (‘Bond B’, more heat‐stable than Bond A) between both proteins and necessity of disruption of Bond B for actin release from myofibrils. 相似文献