The influences of land-use changes on hydrology and riparian environment in a northern Japanese landscape   总被引：10，自引：1，他引：9  

Nagasaka  Akiko  Nakamura  Futoshi 《Landscape Ecology》1999,14(6):543-556

Temporal changes in a hydrological system and riparian ecosystem were examined with reference to land-use conversion in order to clarify the linkages between these two systems. First, the hydrological system of the Toikanbetsu River basin was divided into three components that measure water retention, inundation and conveyance. Variation in the hydrological system was expressed as a basis of delineating the three components and estimating their functions. The rainfall-runoff system was also examined using a model which can predict responses of surface-, subsurface- and base flows on rainfall intensity. Second, areas and fragmentation of the riparian forests, maximum stream temperature in summer and amount of coarse woody debris (CWD) were selected as parameters indicating the condition of the riparian ecosystem. Temporal changes in stream temperature and amount of CWD were estimated using multiple regression analysis and analysis of variance, respectively. The results indicated that the hydrological system has been altered since the 1970s, increasing flood peaks by 1.5–2.5 times and shortening peak appearance by 7 hours. Riparian forests have been disappearing since the 1960s due to extensive development of agricultural lands and river channelization. The summer maximum stream temperature increased from 22 °C in 1947 to 28 °C at present. The amount of CWD should substantially decrease with river channelization and associated forest cutting. Fish favoring cool water, such as masu salmon, could survive in 1947 although they are forced to migrate to cooler forested upstream tributaries now. The ecological systems were closely related to and distinctly altered by land-use. Finally, we propose a new perspective for understanding the two interrelated systems. Riparian ecosystems can be restored by restoring water retention and inundation functions, which also reduce the flood hazard generated by elevated flood peaks.  相似文献   

IL‐12p40 gene expression in lung and hilar lymph nodes of MPS‐resistant pigs     

Akiko Sakuma  Shizuka Sugawara  Hikaru Hidaka  Mitsuru Nakajo  Yoshihito Suda  Tomoyuki Shimazu  Michael T. Rose  Megumi Urakawa  Tao Zhuang  Guoqi Zhao  Kouichi Watanabe  Tomonori Nochi  Haruki Kitazawa  Kazuo Katoh  Keiichi Suzuki  Hisashi Aso 《Animal Science Journal》2020,91(1)

Mycoplasma pneumonia of swine (MPS) is caused by Mycoplasma hyopneumoniae (M.hp) and is a common chronic respiratory disease of pigs. Recently, a genetically selected variant of the Landrace pig (Miyagino L2) has a lower incidence of pulmonary MPS lesions. We investigated the pathological and immunological characteristics of MPS resistance in these pigs (n = 24) by comparing with the normal landrace pig (control: n = 24). The pathological MPS lung lesion score in MPS‐selected landrace pigs was significantly lower than in the control. The gene expression of interleukin (IL)‐12p40, which acts as a chemoattractant and a component of the bioactive cytokines IL‐12 and IL‐23, was significantly higher at the hilar lymph nodes, lung, and spleen in MPS‐selected landrace pigs than in control landrace pigs, and these were negatively correlated with the macroscopic MPS lung lesion score. In summary, we demonstrate that resistance against MPS in Miyagino L2 pigs is associated with IL‐12p40 up‐regulation, in comparison with normal landrace pigs without the MPS vaccine. In addition, a comparative study of macroscopic MPS lung lesions and IL‐12p40 gene expression in lung and hilar lymph nodes may lead to beneficial selection traits for the genetic selection for MPS resistance in pigs.  相似文献   

Allele frequency distribution of the canine dopamine receptor D4 gene exon III and I in 23 breeds     

Ito H  Nara H  Inoue-Murayama M  Shimada MK  Koshimura A  Ueda Y  Kitagawa H  Takeuchi Y  Mori Y  Murayama Y  Morita M  Iwasaki T  Ota K  Tanabe Y  Ito S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(7):815-820

Various canine breeds are remarkably different from each other not only in their sizes and shapes but also in behavioral traits, suggesting that some of them are under genetic control. Although dopaminergic neurotransmission system is considered to affect animal behavior, little is known about related genes in canine. Relations between specific alleles in polymorphic regions of the dopamine receptor D4 gene (DRD4) and personality or psychiatric disorders have been reported in humans, and we first found polymorphism in exon III region of the gene in 4 canine breeds. In this study we surveyed allele frequency distribution in 23 breeds including a total of 1,535 unrelated individuals. In exon III, 8 alleles including a novel allele were identified. A group of breeds in which the alleles 447b, 498 and 549 were frequent tended toward high scores in aggression-related behavioral traits than that with frequent alleles 435 and 447a. Moreover, a polymorphism based on 24 bp insertion/deletion was found in exon I region for the first time in dogs. This information may be of use for candidate gene studies of behavioral variation in dogs.  相似文献   

Secondary erythrocytosis associated with schwannoma in a dog     

Yamauchi A  Ohta T  Okada T  Mochizuki M  Nishimura R  Matsunaga S  Nakayama H  Doi K  Sasaki N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(12):1605-1608

An 11-year-old, spayed female mixed-breed dog showed clinical signs of right forelimb lameness and pain by palpation around the neck. Radiography and computed tomography (CT) revealed an extradural mass at the 6th and 7th cervical vertebrae, which compressed the spinal cord. The mass was surgically removed and histopathologically diagnosed as schwannoma. The dog recovered her normal gait after hemilaminectomy and removal of the mass. Ten months after the surgery, the tumor recurred with absolute erythrocytosis and was surgically removed again. This removal temporarily resolved the erythrocytosis with a decrease in plasma erythropoietin (EPO) concentration. EPO protein was detected immunohistochemically in the tumor cells. Erythrocytosis in this dog may be caused by ectopic EPO produced in the schwannoma tissues.  相似文献   

Effects of nuclear transfer procedures on ES cell cloning efficiency in the mouse     

Yabuuchi A  Yasuda Y  Kato Y  Tsunoda Y 《The Journal of reproduction and development》2004,50(2):263-268

Enucleated oocytes receiving mouse embryonic stem (ES) cells develop into fertile young. The developmental potential to young is low, however, and the rate of postnatal death is high. We examined the effect of various nuclear transfer procedures on the in vitro and in vivo developmental potential of nuclear-transferred oocytes. The potential of oocytes receiving ES cells at M phase to develop into blastocysts after fusion by Sendai virus was high compared with that after direct injection (67% vs. 30%). The developmental potential of oocytes receiving ES cells at the M phase is higher than that of oocytes receiving ES cells at the G(1) phase (30-67% vs. 2-5%). Developmental ability to live young was low in all groups (0-4%). Different activation protocols affected the potential to develop into blastocysts to a different extent (27-62%), but did not affect the potential to develop into live young (0-3%). The present study demonstrated that the various conditions examined did not affect the potential of nuclear-transferred oocytes receiving ES cells to develop into live young or the incidence of postnatal death.  相似文献   

Genomic organization of the chicken T-cell receptor beta chain D-J-C region     

Shigeta A  Sato M  Kawashima T  Horiuchi H  Matsuda H  Furusawa S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(12):1509-1515

We examined overlapping genomic clones containing the chicken T cell receptor (TCR) Dbeta-Jbeta-Cbeta complex, which contains a single diversity segment, four joining segments and four exons that encode the constant region. This sequence comprised 18.3 kb. All four Jbeta sequences possessed typical recombination signal sequences (RSS) with intervening 12-bp spacers at their 5'-ends and splice sites at their 3'-ends. No Jbeta-pseudogenes were identified. TGTG sequences in the RSS heptamer sequences were well conserved, as is the case in mammals. A chicken repeat 1-like sequence was found in the intron region between Jbeta-1336 and Cbeta, and several small repeat sequences were identified in intron regions throughout this cloned genome. As germline sequences revealed complete Jbeta sequences, the CDR3 (complementarity-determining region) sequences of TCRbeta from non-immunized splenocytes were analyzed. Non-coding (N) and palindromic (P) nucleotides were frequently observed at the Dbeta-Jbeta recombination sites. There were differences in length of deletion at the 5'-end of each Jbeta. Deletion of the 5'-end of Jbeta-1280 was particularly short when compared with that of Jbeta-1336, but there were no changes in the length of the CDR3 using any of the four Jbeta sequences.  相似文献   

Canine mdr1 gene mutation in Japan     

Kawabata A  Momoi Y  Inoue-Murayama M  Iwasaki T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(11):1103-1107

Frequency of the 4-bp deletion mutant in canine mdr1 gene was examined in 193 dogs of eight breeds in Japan. The mutant allele was found in Collies, Australian Shepherds, and Shetland Sheepdogs, where its respective frequencies were 58.3%, 33.3%, and 1.2%. The MDR1 protein was detected on peripheral blood mononuclear cells (PBMC) from a MDR1/MDR1 dog, but not on PBMC from a mdr1-1Delta/mdr1-1Delta Collie. Rhodamine 123 was extruded from MDR1/MDR1 lymphocytes. That excretion was inhibited by a MDR1 inhibitor, verapamil. On the other hand, Rh123 excretion was not observed from lymphocytes derived from a mdr1-1Delta/mdr1-1Delta Collie. These results indicated that the mutant mdr1 allele also existed in Collie-breed dogs in Japan at high rates and that mdr1-1Delta /mdr1-1Delta dogs have no functional MDR1.  相似文献   

Effect of anti-ganglioside antibody in experimental Trypanosoma brucei infection in mice     

Tsujimura Y  Watarai S  Uemura A  Ohnishi Y  Kodama H 《Research in veterinary science》2005,78(3):245-247

The effect of antibody against ganglioside antigen on Trypanosoma brucei parasites was examined in vitro and in vivo using anti-ganglioside GM1 (AGM-1) monoclonal antibody. The antibody showed complement-dependent cytotoxicity against T. brucei with mouse complement. Furthermore, mice given AGM-1 were challenged intraperitoneally with T. brucei. Although all non-treated control mice died within six days after infection, all of AGM-1-injected mice had survived by six days post-infection. These data suggest that antibody against ganglioside antigen on T. brucei has potential in protection against T. brucei infection.  相似文献   

Detection of cone dysfunction induced by digoxin in dogs by multicolor electroretinography     

Maehara S  Osawa A  Itoh N  Wakaiki S  Tsuzuki K  Seno T  Kushiro T  Yamashita K  Izumisawa Y  Kotani T 《Veterinary ophthalmology》2005,8(6):407-413

It is difficult to detect discrete cone function with the present conventional electroretinography (ERG) examination. In this study, we developed contact electrodes with a built-in color (red (644 nm), green (525 nm), or blue (470 nm)) light source (color LED-electrode), and evaluated an experimental model of digoxin in the dog. First, 17 normal Beagle dogs were used to determine which electrode works well for color ERG measurement on dogs. Then, color ERG was performed on seven normal Beagle dogs at various points during a 14-day period of digoxin administration. A single daily dose of 0.0125 mg/kg/day, which is within the recommended oral maintenance dosage range for dogs, was administered orally for 2 weeks. Ophthalmic examination, measurement of plasma concentration of digoxin, and color ERG examination were performed. On first examination, amplitudes of all responses were significantly (P < 0.01) lower with the red, than with the blue and green electrodes during ERG recording. In ERG using the red electrode, the standard deviation was large. According to these preliminary results, the red electrode was not used in the experimental dog model with digoxin. In the digoxin administrated animals, no significant change was observed in the ophthalmic examination findings. The digoxin level increased steadily throughout the dosing period but was always within the therapeutic range for dogs. In rod ERG, no abnormalities were detected with any electrode. In standard combined ERG, decreased amplitude of the a-wave was detected with every electrode. In single flash cone ERG, prolongation of implicit time was detected by color ERG with the blue and green electrodes. In 30-Hz flicker ERG, decreased amplitude was detected only by color ERG with the blue electrode. The decreased amplitude and prolonged implicit time recovered after termination of digoxin administration. Cone dysfunction induced by digoxin in the dog was revealed by multicolor ERG using blue and green LED-electrodes. Multi-color ERG was useful for detecting cone type-specific dysfunction in the dog.  相似文献   

C-reactive protein concentration in dogs with various diseases   总被引：1，自引：0，他引：1  

Nakamura M  Takahashi M  Ohno K  Koshino A  Nakashima K  Setoguchi A  Fujino Y  Tsujimoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(2):127-131

To investigate the clinical utility of C-reactive protein (CRP) determination in dogs, its plasma concentration was measured by a laser nephelometric method in 928 dogs with various diseases, and was compared with other inflammatory parameters. CRP concentration was elevated in various inflammatory diseases, this was most frequently observed in cases with neoplastic and immune-mediated diseases. All cases of pyometra, panniculitis, acute pancreatitis, polyarthritis, and hemangiosarcoma showed significantly elevated CRP concentrations. On the other hand, the CRP concentration was elevated only in few cases of neurological diseases such as epilepsy, meningoencephalitis, and hydrocephalus and endocrine diseases such as hypothyroidism, hyperadrenocorticism, and diabetes mellitus. Only a weak correlation was observed between the CRP concentration and white blood cell (WBC) counts (r=0.44) but no correlation with band neutrophil counts. There was no correlation between the CRP and albumin concentrations, but a weak negative correlation (r=-0.40) when excluding chronic intestinal diseases and nephrotic syndrome, which can cause protein loss. Thus, CRP can be useful to detect inflammations that cannot be detected by WBC and, or band neutrophil counts, suggesting that the examination of CRP concentration is essential as routine diagnostic test.  相似文献