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101.
Molecular typing of Japanese strains of Ralstonia solanacearum in relation to the ability to induce a hypersensitive reaction in tobacco 总被引：1，自引：0，他引：1
Yingqin Liu Ayami Kanda Kazutaka Yano Akinori Kiba Yasufumi Hikichi Masataka Aino Akira Kawaguchi Sentaro Mizoguchi Kazuhiro Nakaho Hiroshi Shiomi Yuichi Takikawa Kouhei Ohnishi 《Journal of General Plant Pathology》2009,75(5):369-380
The genetic diversity of 120 Ralstonia solanacearum strains isolated from a variety of host plants across Japan was assessed on the basis of hypersensitive response (HR) in tobacco leaves and phylogenetic analyses of endoglucanase gene egl, hrpB, and gyrB. Phylogenetic analysis of egl revealed that only three strains belonged to phylotype IV, and 117 strains belonged to phylotype I. Partial sequences of HrpB were identical among phylotype I strains except for one strain. Analyses using the partial nucleotide sequences of the gyrB and egl gene fragments grouped phylotype I strains into 11 gyrB and 8 egl types, respectively, whereas analyses using the partial amino acid sequences of GyrB and Egl grouped phylotype I strains into 4 GyrB and 5 Egl types, respectively. Using multilocus sequence typing of GyrB and Egl, we identified 10 unique sequence types within the Japanese phylotype I strains. Strains belonging to the GyrB42 or GyrB66 type caused wilt in tobacco, and strains belonging to GyrB2 or GyrB9 type elicited HR, demonstrating that HR induction in tobacco is genetically differentiated in the Japanese strains of R. solanacearum. 相似文献
102.
When floating vegetation grows on the water surface in a closed water body, the vegetation counteracts the magnitude of the
wind acting on the water surface, and affects the fluid flow and the water quality variation in it. In this study, in order
to investigate the counteraction effect of the luxuriant growth of floating vegetation on the fluid flow and water quality
in the closed water body, we conducted hydraulic experiments on wind-induced flow with density stratification using a wind
tunnel test tank and imitated vegetation. We used imitated vegetation made from polystyrene foam plates instead of using real
floating vegetation. And we experimentally considered the effect of the vegetation using the concept of the wind-induced entrainment
phenomenon. In particular, we focused the effect of the occupation rate and the swaying motion of vegetation. The results
indicated that the swaying motion of imitated floating vegetation had an effect on the scale of entrainment. In addition the
results had the same tendency as the previous study on the wind-induced entrainment phenomenon. 相似文献
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Makara M Shimada A Kawamura N Murase T Morita T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(3):325-327
Neonatal weakness of calves is one of the common reproductive-related problems in captive cetaceans; clinical signs can be observed in the first few hours after delivery. Three 3-day-old bottlenose dolphins died with history of weakness since birth. Pathological study demonstrated purulent bronchopneumonia associated with prominent bacterial colonies and foreign substances in alveoli, suggesting aspiration pneumonia as a cause of neonatal weakness and resultant death of the three calves. 相似文献
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Hirotsune M Haratake A Komiya A Sugita J Tachihara T Komai T Hizume K Ozeki K Ikemoto T 《Journal of agricultural and food chemistry》2005,53(4):948-952
Daily topical applications of the concentrate of sake (CS) have been shown to reduce epidermal barrier disruption in murine skin caused by ultraviolet B (UVB) radiation, while one of the components of sake, ethyl alpha-D-glucoside (alpha-EG), also reduces barrier disruption. We confirmed the effect of oral ingestion of various doses of CS on epidermal barrier disruption caused by UVB irradiation in hairless mice. Then, to identify the effective components, we quantitatively analyzed alpha-EG, organic acids, and glycerol, the main components of CS, and examined the effect of various concentration of each on barrier disruption. alpha-EG and organic acids showed comparable results to CS itself, and transepidermal water loss levels in murine skin were significantly decreased as compared with the control. Furthermore, an investigation of the dose dependency of these agents was performed and the results showed the significant effectiveness of alpha-EG. In addition, red wine concentrate (WC) and beer concentrate (BC) were examined in order to confirm the unique effects of CS. Similar effects were not found with WC and BC. 相似文献