全文获取类型
收费全文 | 109篇 |
免费 | 4篇 |
专业分类
林业 | 3篇 |
农学 | 2篇 |
15篇 | |
综合类 | 3篇 |
农作物 | 6篇 |
水产渔业 | 14篇 |
畜牧兽医 | 36篇 |
植物保护 | 34篇 |
出版年
2023年 | 1篇 |
2022年 | 1篇 |
2021年 | 1篇 |
2020年 | 1篇 |
2019年 | 4篇 |
2018年 | 2篇 |
2016年 | 3篇 |
2015年 | 3篇 |
2014年 | 8篇 |
2013年 | 8篇 |
2012年 | 4篇 |
2011年 | 5篇 |
2010年 | 1篇 |
2009年 | 6篇 |
2008年 | 13篇 |
2007年 | 9篇 |
2006年 | 7篇 |
2005年 | 12篇 |
2004年 | 5篇 |
2003年 | 4篇 |
2002年 | 5篇 |
2000年 | 1篇 |
1999年 | 3篇 |
1998年 | 2篇 |
1995年 | 1篇 |
1992年 | 2篇 |
1983年 | 1篇 |
排序方式: 共有113条查询结果,搜索用时 15 毫秒
21.
Michiru Fukasawa Tokushi Komatsu Yumi Higashiyama Akinori Oshibe 《Animal Science Journal》2018,89(2):488-493
Sleep is one of the essential behaviors for mammals. The aims of this study were to validate the use of accelerometer for measuring sleeping posture of cattle. Duration of sleeping posture of seven Japanese Black cows from 19.00 to 07.00 hours was measured by both accelerometer and video, and a total of 67 accelerometer and video measurement sets were collected. We calculated Cohen's κ coefficient between accelerometer and video measurements and 91.5% of the κ‐values were >0.80. Intra‐ and inter‐observer coefficient of variance showed that specific acceleration waveform patterns of sleeping posture could be easily and accurately detected by independent observers. There were no significant differences in the frequency of sleeping posture occurrences between accelerometer and video measurements. We compared averaged sleeping posture bout, and the total sleeping posture time between accelerometer and video measurements using regression. In each trait, the slope was close to 1 and the intercept was not different from 0, which showed a strong agreement between accelerometer and video measurements. This shows that an accelerometer could accurately detect sleeping postures of cattle. We conclude that adequate measurements of sleeping postures can be made using an accelerometer. 相似文献
22.
Masayasu Saruta Yoshitake Takada Akio Kikuchi Tetsusya Yamada Kunihiko Komatsu Takashi Sayama Masao Ishimoto Akinori Okabe 《Breeding Science》2012,61(5):625-630
The peanut stunt virus (PSV) causes yield losses in soybean and reduced seed quality due to seed mottling. The objectives of this study were to determine the phenotypic reactions of soybean germplasms to inoculation with two PSV isolates (PSV-K, PSV-T), the inheritance of PSV resistance in soybean cultivars, and the locus of the PSV resistance gene. We investigated the PSV resistance of 132 soybean cultivars to both PSV isolates; of these, 73 cultivars exhibited resistance to both PSV isolates. Three resistant cultivars (Harosoy, Tsurunotamago 1 and Hyuga) were crossed with the susceptible cultivar Enrei. The crosses were evaluated in the F1, F2 and F2:3 generations for their reactions to inoculation with the two PSV isolates. In an allelism test, we crossed Harosoy and Tsurunotamago 1 with the resistant cultivar Hyuga. The results revealed that PSV resistance in these cultivars is controlled by a single dominant gene at the same locus. We have proposed Rpsv1, as the name of the resistance gene in Hyuga. We also constructed a linkage map using recombinant inbred lines between Hyuga × Enrei using 176 SSR markers. We mapped Rpsv1 near the Satt435 locus on soybean chromosome 7. 相似文献
23.
Morita T Takahashi M Takeuchi T Hikasa Y Ikeda S Sawada M Sato K Shibahara T Shimada A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(11):1119-1126
Intracerebral microdialysis combined with electroencephalographic recordings was performed on 4 dogs of a familial idiopathic epileptic Shetland sheepdog colony to identify the kinds of neurotransmitters responsible for seizure activity. Immunohistochemistry using glutamate (Glu), glutamate transporter (GLT-1 and GLAST), and glutamine synthetase (GS) antibodies was also carried out on the cerebrum of four familial dogs that died of status epilepticus (SE). High values for extracellular levels of Glu and aspartate (ASP) were detected in association with an increased number of spikes and sharp waves during hyperventilation in 3 of 4 the familial epileptic dogs. The values of other amino acids analyzed were not altered in any of the familial epileptic dogs. Immunohistochemically, Glu-positive granules were occasionally found in the perineuronal spaces of the cerebral cortex in 3 of the familial epileptic dogs that died of SE. Immunostains for GLT-1 antibody predominantly decreased in the cerebral cortex and lateral nucleus of the thalamus in all the dogs that died of SE, whereas there were no differences detected in immunolabellings for GLAST and GS antibodies between familial epileptic dogs and controls. These results suggest that an extracellular release of both Glu and Asp may play an important role in the occurrence of seizure activity in this epileptic colony, and that a decreased expression of astrocytic GLT-1 may be related to development of SE. 相似文献
24.
25.
Akinori Kiba Takako Ohgawara Kazuhiro Toyoda Miho Inoue-Ozaki Tadahiro Takeda Uppalapati Srinivasa Rao Toshiaki Kato Yuki Ichinose Tomonori Shiraishi 《Journal of General Plant Pathology》2006,72(4):228-237
In the plant cell wall of Pisum sativum seedlings, we found an NTPase (E.C. 3.6.1.5.) with ATP-hydrolyzing activity that was regulated by an elicitor and suppressors
of defense from pea pathogen Mycosphaerella pinodes. The ATPase-rich fraction was purified from pea cell walls by NaCl solubilization, ammonium sulfate precipitation, and chromatography
with an ATP-conjugated agarose column and an anion-exchange column. The specific activity of the final ATPase-rich fraction
increased 600-fold over that of the initial NaCl-solubilized fraction. The purified ATPase-rich fraction also had peroxidase
activity and generated superoxide, both of which were regulated by the M. pinodes elicitor and suppressor (supprescins). Active staining and Western blot analysis also showed that the ATPase was copurified
along with peroxidases. In this fraction, a biotinylated elicitor and the supprescins were bound primarily and specifically
to ca. 55-kDa protein (CWP-55) with an N-terminal amino acid sequence of QEEISSYAVVFDA. The cDNA clone of CWP-55 contained five ACR domains, which are conserved in
the apyrases (NTPases), and the protein is identical to a pea NTPase cDNA (GenBank accession AB071369). Based on these results, we discuss a role for the plant cell wall in recognizing exogenous
signal molecules. 相似文献
26.
Sugiyama A Morita T Shimada A Tsuka T Okamoto Y Takeuchi T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(7):739-742
A 10-year-old Golden Retriever dog had a solitary tumor mass arising from the greater omentum. Histologically, the tumor showed varying cellularity and patterns of cellular arrangement. In dense cellular areas, spindle-shaped cells were arranged in interlacing bundles. The sparse cellular area was characterized by loosely arranged fusiform cells. The neoplastic cells frequently contained PAS-positive eosinophilic globules in the cytoplasm, and mitotic figures were frequently observed. The tumor cells were positive to vimentin, S-100 protein, glial fibrillary acidic protein, myelin basic protein, neuron-specific enolase and myoglobin. The present tumor was diagnosed as a malignant peripheral nerve sheath tumor (MPNST) with eosinophilic cytoplasmic globules arising from the greater omentum. To our knowledge, this may be the first case of primary omental MPNST in domestic animals. 相似文献
27.
Ulloa Ullo CM Yoshizawa M Komoriya E Mitsui A Nagai T Kikuchi K 《The Journal of reproduction and development》2008,54(1):22-29
The present study was conducted to determine the relationship between embryonic development speed at different stages (the cleaved stage at 52 h and the blastocyst stage at 6 days post insemination) and incidences of chromosome abnormalities in in vitro produced porcine embryos. Porcine oocytes were collected from 3-6-mm ovarian follicles obtained at a slaughterhouse and matured in modified NCSU-37 medium for 44-46 h. Following in vitro fertilization with a final concentration of 1 x 10(5) sperm/ml for 3 h, all oocytes were cultured in vitro for 52 h. Day-2 (52 h after insemination) embryos were classified according to their cleaved stages into 2-cell, 3- to 4-cell, 5- to 8-cell, and >8-cell stages; these were cultured separately for additional 4 days (Day 6). The resultant Day-6 blastocysts were classified according to the morphological diameter into 3 grades: Grade A, expanded blastocysts; Grade B, expanding blastocysts; and Grade C, early blastocysts. They were then analyzed chromosomally. The 3- to 4-cell and 5- to 8-cell embryos had significantly high blastocyst development rates (46.1 and 36.9%, respectively), and these blastocysts contained significantly more cells (40.2 and 42.4 cells, respectively) than those derived from 2-cell embryos and >8-cell embryos (28.6 and 26.5 cells, respectively). The incidence of chromosomal abnormalities was significantly higher in the blastocysts derived from 2-cell and >8-cell stage embryos than in the blastocysts derived from the other stage embryos. Furthermore, the grade A blastocysts had the lowest incidence of chromosomal abnormalities (35.3%) and contained the most cells (48.7 cells). Porcine in vitro production (IVP) yielded a high blastocyst rate and an excellent embryo quality when 3- to 4-cell and 5- to 8-cell stage embryos were selected on Day 2 after insemination. The same criteria yielded a higher quality of expanded blastocysts based on the stage of embryo development and morphology. 相似文献
28.
29.
Kenji Shinohara Akinori Nagao Shiro Okuda Kaoru Niiyama Toyonobu Sugawa 《Journal of Forest Research》1998,3(1):55-60
The effects of temperature on the growth characteristics of a Japanese willow (Salix gilgiana Seemen) were studied with respect to dry matter production, wood anatomy and the net photosynthetic rates of leaves. Clonal
materials were grown in natural-light rooms (phytotrons) at 30°C/25°C, at 25°C/20°C or at 20°C/15°C (day/night: 12 h/12 h),
respectively. The elongation rate of shoots from the beginning of June to the end of July was approximately 22 cm per week
under the three different sets of conditions. The shoots reached approximately 3 m in height after 4 months of growth. Total
dry matter production and dry weights of stems did not differ very much among the three sets of temperature conditions after
9 months of growth. The dry weight of leaves increased at high temperatures while that of roots decreased. The number of vessels
increased significantly at 30°C/25°C and 25°C/20°C, whereas the diameters of vessels and fibers, and the double wall thickness
of fibers did not vary markedly under all three temperature regimes. The net photosynthetic rate and the respiration rate
in mature leaves were highest at 30°C/25°C, and both activities increased with increases in growth temperature. The increase
in the photosynthetic rate was much smaller than that in the respiration rate. Individual plants had been completely adapted
to the respective growth temperatures, as judged from the decrease in the photosynthetic rate after the transfer to different
temperatures.
Part of this work was reported at the 102nd Annual Meeting of the Japanese Forestry Society (Nagoya, Japan, 1991).
This work was supported by Grants-in-Aid (Integrated Research Program for Effective Use of Biological Activities to Create
New Demand) from the Ministry of Agriculture, forestry and Fisheries of Japan (BRP 97-IV-B-10). 相似文献
30.
Understanding of density‐dependent effects is key to achieving sustainable management of self‐regulating biological resources such as fish stocks. Traditionally, density‐dependent effects on population abundance in fish have been considered to occur from hatching to recruitment, based on the paradigm of proportionality between spawning stock biomass and total egg production. Here, we demonstrate how the existence of intraspecific and interspecific density dependence in egg production changes the current understanding of density‐dependent processes in the life history of fish, by disentangling density‐dependent effects on egg production and survival from egg to recruitment, using sardine (Sardinops melanostictus, Clupeidae) and anchovy (Engraulis japonicus, Engraulidae) as model species. For sardine, strong intraspecific density‐dependent effects occurred in egg production, but no density‐dependent effects occurred or if any they were weak enough to be masked by environmental factors from hatching to recruitment. In contrast, for anchovy, interspecific density‐dependent effects occurred in egg production. In the survival after hatching, anchovy experienced stronger intraspecific density‐dependent effects than currently recognized. This analysis could overturn the current understanding of density‐dependent effects in the life history, highlighting contrasts between the effects on individual quality and population abundance and between the model species. We propose to reconsider the basis of fisheries management and recruitment studies based on the revised understanding of density‐dependent effects in the life history of the respective species. 相似文献