α-Melanocyte-stimulating hormone directly increases the plasma calcitonin level and involves calcium metabolism in goldfish     

Ikari  Takahiro  Kobayashi  Yuki  Kitani  Yoichiro  Sekiguchi  Toshio  Endo  Masato  Kambegawa  Akira  Asahina  Kiyoshi  Hattori  Atsuhiko  Tabuchi  Yoshiaki  Amornsakun  Thumronk  Mizusawa  Kanta  Takahashi  Akiyoshi  Suzuki  Nobuo 《International Aquatic Research》2018,10(3):283-292

International Aquatic Research - The effects of α-melanocyte-stimulating hormone (α-MSH) on calcium metabolism were examined with goldfish. The scales on the left side of goldfish bodies...  相似文献   

Relationships among the chalkiness,kernel size and endosperm cell morphology of rice kernels at different spikelet positions within a panicle     

Akira Miyazaki  Akiko Ikeda  Junichi Yonemaru  Satoshi Morita  Yoshinori Yamamoto 《Plant Production Science》2018,21(3):225-232

The occurrence of chalky kernels in rice is causally related to kernel size and endosperm morphology. This study aimed to investigate the occurrence of chalky kernels by analyzing kernel size, and the number and area of endosperm cells. Spikelets were sampled from upper and middle primary branches and lower secondary branches in a panicle, and divided into four categories: upper, middle, and lower perfect (PF) kernels and lower milky-white (MW) kernels. On the lower secondary branches, there was a higher percentage of chalky kernels, with smaller kernel lengths, widths and thicknesses, than the kernels on the upper and middle primary branches. MW kernels were smaller in size than PF kernels even on the same lower secondary branches. Regardless of grain appearance quality traits, the total areas of endosperm cross sections in lower kernels were significantly smaller than in upper kernels owing to the decreased cell area, and there was a significant negative correlation between the number of cells and average cell area. When the numbers and the areas of cells were analyzed using angular 30° intervals from the line connecting the center point and the dorsal vascular bundle, the MW kernels had significantly less cells than PF kernels near the ventral side at 120–180°. Thus, the decrease in the number of cells near the ventral side was a main causal factor in the decrease in MW kernel widths compared with PF kernel widths, and this suggested that cell division in MW kernels was inhibited at the early grain-filling stage.  相似文献   

Drought-induced root plasticity of two upland NERICA varieties under conditions with contrasting soil depth characteristics     

Daniel Makori Menge  Emi Kameoka  Mana Kano-Nakata  Akira Yamauchi  Shuichi Asanuma  Hidetoshi Asai 《Plant Production Science》2016,19(3):389-400

To identify differences in root plasticity patterns of two upland New Rice for Africa (NERICA) varieties, NERICA 1 and 4, in response to drought under conditions with contrasting soil profile characteristics, soil moisture gradients were imposed using a sloping bed system with depths ranging 30–65 cm and a line-source sprinkler system with a uniformly shallow soil layer of 20 cm depth. Varietal differences in shoot and root growths were identified only under moderate drought conditions, 11–18% v/v soil moisture content. Further, under moderate drought soil conditions where roots could penetrate into the deep soil layer, deep root development was greater in NERICA 4 than in NERICA 1, which contributed to maintaining dry matter production. However, under soil conditions with underground impediment to deep root development, higher shoot dry weight was noted for NERICA 1 than for NERICA 4 at 11–18% v/v soil moisture content, which was attributed to increased lateral root development in the shallow soil layer in NERICA 1. Enhanced lateral root development in the 0–20-cm soil layer was identified in NERICA 1 even under soil conditions without an impediment to deep root development; however, this did not contribute to maintaining dry matter production in upland rice. Thus, we show different root developmental traits associated with drought avoidance in the two NERICA varieties, and that desirable root traits for upland rice cultivation vary depending on the target soil environment, such as the distribution of soil moisture and root penetration resistance.  相似文献   

Biological control of potato scab and antibiosis by antagonistic Streptomyces sp. WoRs-501     

Yuki O. Kobayashi  Akira Kobayashi  Masayuki Maeda  Nobutaka Someya  Shigehito Takenaka 《Journal of General Plant Pathology》2015,81(6):439-448

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Biological control agent Agrobacterium vitis strain ARK-1 suppresses expression of the virD2 and virE2 genes in tumorigenic A. vitis     

Akira Kawaguchi 《European journal of plant pathology / European Foundation for Plant Pathology》2015,143(4):789-799

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Identification of a 4-Deoxy-l-erythro-5-hexoseulose Uronic Acid Reductase,FlRed, in an Alginolytic Bacterium Flavobacterium sp. Strain UMI-01     

Akira Inoue  Ryuji Nishiyama  Shogo Mochizuki  Takao Ojima 《Marine drugs》2015,13(1):493-508

In alginate-assimilating bacteria, alginate is depolymerized to unsaturated monosaccharide by the actions of endolytic and exolytic alginate lyases (EC 4.2.2.3 and EC 4.2.2.11). The monosaccharide is non-enzymatically converted to 4-deoxy-l-erythro-5-hexoseulose uronic acid (DEH), then reduced to 2-keto-3-deoxy-d-gluconate (KDG) by a specific reductase, and metabolized through the Entner–Doudoroff pathway. Recently, the NADPH-dependent reductase A1-R that belongs to short-chain dehydrogenases/reductases (SDR) superfamily was identified as the DEH-reductase in Sphingomonas sp. A1. We have subsequently noticed that an SDR-like enzyme gene, flred, occurred in the genome of an alginolytic bacterium Flavobacterium sp. strain UMI-01. In the present study, we report on the deduced amino-acid sequence of flred and DEH-reducing activity of recombinant FlRed. The deduced amino-acid sequence of flred comprised 254 residues and showed 34% amino-acid identities to that of A1-R from Sphingomonas sp. A1 and 80%–88% to those of SDR-like enzymes from several alginolytic bacteria. Common sequence motifs of SDR-superfamily enzymes, e.g., the catalytic tetrad Asn-Lys-Tyr-Ser and the cofactor-binding sequence Thr-Gly-x-x-x-Gly-x-Gly in Rossmann fold, were completely conserved in FlRed. On the other hand, an Arg residue that determined the NADPH-specificity of Sphingomonas A1-R was replaced by Glu in FlRed. Thus, we investigated cofactor-preference of FlRed using a recombinant enzyme. As a result, the recombinant FlRed (recFlRed) was found to show high specificity to NADH. recFlRed exhibited practically no activity toward variety of aldehyde, ketone, keto ester, keto acid and aldose substrates except for DEH. On the basis of these results, we conclude that FlRed is the NADH-dependent DEH-specific SDR of Flavobacterium sp. strain UMI-01.  相似文献   

Entry Inhibition of Influenza Viruses with High Mannose Binding Lectin ESA-2 from the Red Alga Eucheuma serra through the Recognition of Viral Hemagglutinin     

Yuichiro Sato  Kinjiro Morimoto  Takanori Kubo  Takemasa Sakaguchi  Akira Nishizono  Makoto Hirayama  Kanji Hori 《Marine drugs》2015,13(6):3454-3465

Lectin sensitivity of the recent pandemic influenza A virus (H1N1-2009) was screened for 12 lectins with various carbohydrate specificity by a neutral red dye uptake assay with MDCK cells. Among them, a high mannose (HM)-binding anti-HIV lectin, ESA-2 from the red alga Eucheuma serra, showed the highest inhibition against infection with an EC₅₀ of 12.4 nM. Moreover, ESA-2 exhibited a wide range of antiviral spectrum against various influenza strains with EC₅₀s of pico molar to low nanomolar levels. Besides ESA-2, HM-binding plant lectin ConA, fucose-binding lectins such as fungal AOL from Aspergillus oryzae and AAL from Aleuria aurantia were active against H1N1-2009, but the potency of inhibition was of less magnitude compared with ESA-2. Direct interaction between ESA-2 and a viral envelope glycoprotein, hemagglutinin (HA), was demonstrated by ELISA assay. This interaction was effectively suppressed by glycoproteins bearing HM-glycans, indicating that ESA-2 binds to the HA of influenza virus through HM-glycans. Upon treatment with ESA-2, no viral antigens were detected in the host cells, indicating that ESA-2 inhibited the initial steps of virus entry into the cells. ESA-2 would thus be useful as a novel microbicide to prevent penetration of viruses such as HIV and influenza viruses to the host cells.  相似文献   

A new method for evaluating symplastic cadmium absorption in the roots of Solanum melongena using enriched isotopes ¹¹³Cd and ¹¹⁴Cd     

Shinsuke MORI  Akira KAWASAKI  Satoru ISHIKAWA  Tomohito ARAO 《Soil Science and Plant Nutrition》2009,55(2):294-299

Radioisotope techniques are well known as methods for evaluating symplastic ion absorption in roots. In the present study, a new method for evaluating symplastic cadmium (Cd) absorption in plant roots was developed using the enriched isotopes ¹¹³Cd and ¹¹⁴Cd. Seedlings of Solanum melongena were exposed to an enriched isotope solution of ¹¹³Cd at 25°C for 30 min. The roots were excised from each seedling and were then immersed in a cold buffer solution without Cd at 2°C for 120 min to suppress the metabolic activity of the roots. Finally, the roots were treated with a cold buffer solution containing enriched stable isotope ¹¹⁴Cd at 2°C for 120 min, whereby the apoplastically bound ¹¹³Cd was desorbed. We tested the validity of our method for evaluating symplastic Cd in roots compared with the conventional method based on differences in the amount of Cd absorbed at 2°C and 25°C using unlabeled Cd. There was no difference in the symplastic Cd content of the roots between the two methods. These results indicate that it is possible to evaluate the symplastic Cd content in roots using the enriched isotopes ¹¹³Cd and ¹¹⁴Cd.  相似文献   

海岸湿地针叶林-阔叶林突变临界维持机制研究     

Shiro Tsuyuzaki Akira Haraguchi 《林业研究》2009,20(2)

日本北海道东部有两块发展成熟的湿地森林,分别是具有氮固定作用的日本桤木（Alnusjaponica Steud）组成的阔叶林和库页云杉（Piceaglehnii Masters）构成的针叶林,而且两块林地间具有突变的边界。为了阐明突变边界的维持机制,本文研究了森林剖面、水位、地下水、降雨化学、与微环境有关的幼苗建成格局及种子迁移。研究表明,地下水位剖面并不能充分地解释突转临界的形成,但是,两种林地的地下水化学成分存在显著差异,具体表现为库页云杉林地下水中EC,Na^＋,心,Mg^2＋,Ca^2＋和Cl^-含量明显要高于日本桤木林的,而地下水pH值又较低。库页云杉林降雨中富含Na^＋,Ca^2＋和Cl^-,表明降雨是导致两种林分土壤表层水化学成分差异的主要原因。六月末,库页云杉林的太阳辐射少于2．2MJ·m^-2．d^-1;而日本桤木林太阳辐射强度为1．0—3．7MJ·m^-2.d^-1。土壤表层苔藓主要是泥炭藓,在库页云杉林内覆盖率为60％,而日本桤木林的仅为10％：这一差异与土壤表层水pH值有关。有70％株高在1．3米以下的库页云杉苗木建立在苔藓层上。播种造林实验表明,在库页云杉林2个树种的种子萌发率和幼苗存活率都较高。因此,库页云杉在日本桤木林内繁殖能力较弱,主要是缺少适宜的微环境和较低的苗木建成能力。日本桤木仅靠再萌发繁殖,在两种林分内幼苗较少;此外,日本桤木种子向库页云杉林内的迁移也受到较大的限制,加之库页云杉林内太阳辐射强度较低,导致日本桤木幼苗存活率较低。基于以上结果,我们认为日本桤木与库页云杉形成了各自特有的生长环境,而对彼此的生长环境不适应,抑制了自身种苗向对方林分的扩张和自然再造过程。图9表1参32。  相似文献   

Species diversity of the marine diatom genus Skeletonema in Japanese brackish water areas     

Machiko Yamada  Mayuko Otsubo  Yuki Tsutsumi  Chiaki Mizota  Naoki Iida  Kazuma Okamura  Masashi Kodama  Akira Umehara 《Fisheries Science》2013,79(6):923-934

The genus Skeletonema includes phytoplankton species that are important primary producers in marine food chains. Brackish waters have been reported to be one of the important habitats of some species of Skeletonema. To elucidate the species diversity of Skeletonema in brackish waters, we investigated three Japanese brackish bodies of water: the coastal waters of Toyama Bay, a tidal area of the Chikugo River, and a constructed reservoir in Isahaya Bay. We used molecular analysis based on large subunit rDNA and fine morphological structure to identify species. Skeletonema costatum s.s. (sensu stricto) was isolated at salinities as low as 0.6, but Skeletonema dohrnii, Skeletonema subsalsum, and Skeletonema tropicum were not found at salinities below 11.0. S. costatum s.s. could survive transfer from a medium with a salinity of 15 to a salinity of 2, but S. dohrnii did not survive in the same experiment. Only S. costatum s.s. germinated from the sediment of a reservoir in which the salinity was 0.1–1.4; incubation conditions included temperatures of 10, 15, 20, 25, and 30 °C and salinities of 5 and 30. Skeletonema costatum s.s. was identified as the species most adaptable to low-level salinity variations throughout its lifecycle.  相似文献