Comparison of heat production and plasma lipid metabolites between meat- and egg-types of Nagoya breed chicken during embryonic development     

Momoka SATO  Kenji NODA  Katsutoshi KINO  Akihiro NAKAMURA  Mitsuhiro FURUSE 《Animal Science Journal》2007,78(6):613-618

We compared heat production (HP) and plasma lipid metabolites between meat‐ and egg‐types of Nagoya breed chickens during embryonic development. To investigate HP and respiratory quotient, oxygen consumption and carbon dioxide production were measured using an open‐circuit calorimeter system. HP was significantly lower in meat‐ than in egg‐type chickens during embryonic development, and HP gradually decreased with developmental stage in both types. The respiratory quotient was constant at approximately 0.68 at every embryonic stage investigated, and the value was similar in both types. Plasma concentrations of triacylglycerol, nonesterified fatty acid, glycerol and D‐3‐hydroxybutyrate, which are associated with lipid metabolism, were similar in both types during embryonic development. These results demonstrate that chicken embryos selected for rapid growth of Nagoya breed have characteristic lower HP, and that when selecting chickens for rapid growth, HP is an important parameter during embryonic stages.  相似文献   

Immunohistochemistry and molecular epidemiology of avian paramyxovirus 1 from formalin-fixed and paraffin-embedded sections of Japanese doves (Columba livia) affected with neurological signs     

Kikuyasu NAKAMURA  Hideo FUJIMORI  Akiko KOYAMA  Trinh Quang DAI  Kunitoshi IMAI  Mitsutaka IKEZAWA  Yu YAMAMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):837-841

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Computed tomography and magnetic resonance imaging of thoracic chordoma in a Bengal tiger (Panthera tigris tigris)     

Toshie ISERI  Junichiro SHIMIZU  Hideo AKIYOSHI  Kayo KUSUDA  Akiyoshi HAYASHI  Keiichiro MIE  Takeshi IZAWA  Mitsuru KUWAMURA  Jyoji YAMATE  Yuka FUJIMOTO  Fumihito OHASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):857-860

A Bengal tiger was presented for evaluation of weakness, ataxia and inappetance. Computed tomography (CT) and magnetic resonance imaging (MRI) revealed a mass extending from the T7-8 vertebral body to the left rib and compressing the spinal cord. On CT, the bone destruction and sequestrum were shown. On MRI, the multilobulated mass appeared hypo- to isointense in T1-weighted and hyperintense in T2-weighted images. The tiger died after imaging, most likely from renal failure. Chordoma without metastasis was diagnosed on necropsy. The imaging characteristics were similar to those found in chordoma in humans. This report describes the use of CT and MRI in an exotic species.  相似文献   

Analysis of a pair of END+ and END? viruses derived from the same bovine viral diarrhea virus stock reveals the amino acid determinants in Npro responsible for inhibition of type I interferon production     

Takashi KOZASA  Yuri ABE  Kazuya MITSUHASHI  Tomokazu TAMURA  Hiroshi AOKI  Masatoshi ISHIMARU  Shigeyuki NAKAMURA  Masatoshi OKAMATSU  Hiroshi KIDA  Yoshihiro SAKODA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(5):511-518

The Exaltation of Newcastle disease virus (END) phenomenon is induced by the inhibition of type I interferon in pestivirus-infected cells in vitro, via proteasomal degradation of cellular interferon regulatory factor (IRF)-3 with the property of the viral autoprotease protein N^pro. Reportedly, the amino acid residues in the zinc-binding TRASH motif of N^pro determine the difference in characteristics between END-phenomenon-positive (END⁺) and END-phenomenon-negative (END⁻) classical swine fever viruses (CSFVs). However, the basic mechanism underlying this function in bovine viral diarrhea virus (BVDV) has not been elucidated from the genomic differences between END⁺ and END⁻ viruses using reverse genetics till date. In the present study, comparison of complete genome sequences of a pair of END⁺ and END⁻ viruses isolated from the same virus stock revealed that there were only four amino acid substitutions (D136G, I2623V, D3148G and D3502Y) between two viruses. Based on these differences, viruses with and without mutations at these positions were generated using reverse genetics. The END assay, measurements of induced type I interferon and IRF-3 detection in cells infected with these viruses revealed that the aspartic acid at position 136 in the zinc-binding TRASH motif of N^pro was required to inhibit the production of type I interferon via the degradation of cellular IRF-3, consistently with CSFV.  相似文献   

Analysis of the equine ovarian structure during the first twelve months of life by three-dimensional internal structure microscopy     

Mamiko ONO  Hiroki AKUZAWA  Yasuo NAMBO  Yuuko HIRANO  Junpei KIMURA  Satoko TAKEMOTO  Sakiko NAKAMURA  Hideo YOKOTA  Ryutaro HIMENO  Tohru HIGUCHI  Tadatoshi OHTAKI  Shigehisa TSUMAGARI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1599-1603

A three-dimensional internal structure microscopy (3D-ISM) can clarify the anatomical arrangement of internal structures of equine ovaries. In this study, morphological changes of the equine ovary over the first 12 months of life were investigated by 3D-ISM in 59 fillies and by histological analysis in 2 fillies. The weight and volume of the paired ovaries initially decreased from 0 to 1 months to 2 to 3 months of age and then significantly increased at 8 to 12 months of age. The ovulation fossa was first observed around the 3rd month and became evident after the 6th month. The number of follicles with a diameter of ≥10 mm and the diameter of the largest follicle increased gradually after 6 months of age. On a volume basis, the medulla accounted for nearly 90% of the whole ovary at 0 to 1 months of age, but significantly decreased from 2 to 3 months of age. The volume of the cortex increased progressively after birth and reached approximately 60% of the total volume at 8 to 12 months of age. This significant development of the cortex coincided with the increased number and size of large follicles observed from 6 months of age. These results suggest that the development of the cortex plays a role in the maturation of the follicles and the equine ovary undergoes substantial morphological changes postnatally until puberty.  相似文献   

Factors affecting the seed germination and seedling emergence of redflower ragleaf (Crassocephalum crepidioides)     

ICHIRO NAKAMURA  MOHAMMAD AMZAD HOSSAIN 《Weed Biology and Management》2009,9(4):315-322

Redflower ragleaf (Crassocephalum crepidioides) is a weed, as well as a minor vegetable, in tropical and subtropical regions of the world. The influence of environmental factors and seed conditions on the germination and emergence of redflower ragleaf have been evaluated in order to help understand its distribution and to develop effective management strategies. The seeds germinated at a constant temperature in the range of 10–30°C and reached a maximum at 15–20°C. The highest germination rate was recorded at an alternating temperature of 20/15°C (day/night). The seeds germinated over a wide pH range (2–12), with the highest germination rate at between 4 and 10. Germination under saturated and flooded conditions was also high. The germination of seeds from opened (mature) capitula was significantly higher than from partially opened or unopened capitula. The germination of seeds without a pappus was significantly higher than for seeds with a pappus. The germination rate of 1 year old seeds decreased drastically when compared to that of freshly harvested seeds. The seedling emergence rate was ～63% for those seeds placed on the soil surface, but no seedling emerged from a depth of ≥1 cm. These results indicate that redflower ragleaf seeds can germinate in various environmental conditions, but that the percentage that germinates will be different in different environments. Regeneration could be effectively prevented by at least a 1 cm soil covering or by destroying the plant before the capitula open. In contrast, freshly harvested seeds from opened capitula should be sown on the soil surface when redflower ragleaf is to be cultivated as a vegetable.  相似文献   

Interaction of orexin/hypocretin-like immunoreactive neurons with melanin-concentrating hormone and α-melanocyte-stimulating hormone neurons in brain of a pleuronectiform fish, barfin flounder   总被引：1，自引：0，他引：1  

Noriko Amiya  Masafumi Amano  Masayuki Iigo  Takeshi Yamanome  Akiyoshi Takahashi  Kunio Yamamori 《Fisheries Science》2008,74(5):1040-1046

Immunohistochemical localization of orexin/hypocretin in the brain of a pleuronectiform fish, the barfin flounder Verasper moseri was examined as the first step in unraveling the possible function of the hormone in the brain. Orexin-A-like immunoreactive (ir) cell bodies were found to be located in the nucleus posterioris periventricularis (NPPv) of the hypothalamus, and orexin-A-like-ir fibers were detected not only in the hypothalamus but also extensively throughout the brain. The orexin-A-like-ir cell bodies did not project their fibers to the pituitary gland. Since melaninconcentrating hormone (MCH) and α-melanocyte-stimulating hormone (α-MSH) are suggested to regulate food intake in addition to orexin/hypocretin in the teleost fish, it was examined whether neural connections exist between orexin neurons and the MCH and α-MSH neurons in the barfin flounder brain by using double-staining immunohistochemistry. Some orexin-A-like-ir fibers were in close contact with the MCH-ir and α-MSH-ir cell bodies in the hypothalamus. Moreover, a few MCH-ir and α-MSH-ir fibers were in close contact with the orexin-A-like-ir cell bodies in the hypothalamus. These results suggest that reciprocal connections exist between the orexin and MCH neurons and between the orexin and α-MSH neurons in the brain of the barfin flounder.  相似文献   

The urinary lipid profile in cats with idiopathic cystitis     

Shinya TAKENOUCHI  Yui KOBAYASHI  Tatsuya SHINOZAKI  Koji KOBAYASHI  Tatsuro NAKAMURA  Tomohiro YONEZAWA  Takahisa MURATA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(5):689

Although feline idiopathic cystitis (FIC) distresses of many cats, its pathogenesis is unknown and the diagnosis is challenging. Polyunsaturated fatty acids (PUFAs) are metabolized into various lipid mediators. Lipid mediators such as prostaglandins (PGs) modulate inflammation and many of them are excreted into the urine. Thus, the investigation of the urinary lipid profile may reveal pathogenesis and help diagnosis of FIC. We collected urine samples from five FIC cats by spontaneous urination and analyzed 158 types of lipid mediators in urines using liquid chromatography-mass spectrometry. The urinary levels of PUFAs were higher in FIC compared to those of the healthy group. The excretions of a major inflammatory mediator, PGD₂, were less in FIC. Other well-known inflammatory mediators such as PGE₂, PGI₂, and their metabolites did not show a difference. In contrast, the levels of PGF_2α and its 2 metabolites and PGF_3α were higher in FIC. These results may provide new insights into the future management of cat FIC.  相似文献   

Expression patterns of the slit subfamily mRNA in canine malignant mammary tumors     

Tanno T  Tanaka Y  Sugiura T  Akiyoshi H  Takenaka S  Kuwamura M  Yamate J  Ohashi F  Kubo K  Tsuyama S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(11):1173-1177

Slit, a secreted protein, functions as a chemorepellent factor in axon guidance and neuronal migration and as an inhibitor in leukocyte chemotaxis. In humans, slit2 protein attracts endothelial cells and promotes tube formation in the tumor angiogenic mechanism. In this study, we cloned a part of the canine slit subfamily and examined the expression of slit subfamily mRNAs in 3 normal canine mammary glands and 11 mammary tumor samples by RT-PCR. The cloned part of the slit gene sequences showed high similarity to those of the human, mouse, and rat. The mRNAs were expressed at low levels in the normal mammary gland. The expression levels of slit1 mRNA were low in both the normal and tumor tissues. In contrast, the expression of slit2 mRNA increased in most of the malignant mammary tumors, and an increase in slit3 mRNA expression was observed in 2 of the malignant mixed tumors. These results suggest that the expression of slit2 plays an important role in tumor angiogenesis in canine mammary gland tumors and that slit2 can be a putative marker for malignancy diagnosis of these tumors.  相似文献   

Spinal cord trauma in a cat caused by ingestion of a splinter     

Tanaka T  Akiyoshi H  Shimazaki H  Sato A  Inada M  Kiyomiya K  Shimizu J  Cao Z  Li Y  Hayashi A  Sone K  Kitai T  Ohashi F 《Journal of Feline Medicine and Surgery》2012,14(2):155-157

A 5-month-old male Abyssinian presented with right hemiplegia. Cervical radiographs, haematology and serum biochemistry were normal. A foreign body was identified on computed tomography (CT) and magnetic resonance imaging (MRI). Removal of the foreign body was carried out via a dorsal approach between the occipital bone and the C1 vertebral body using intra-operative CT. No complications were encountered. Thirty days after surgery, the cat was ambulatory.  相似文献